12 results about "Viral growth" patented technology

The present application relates to an avian cell line capable of supporting viral growth of Marek's Disease Virus (MDV), including Herpes Virus of Turkeys (HVT), methods of producing such cell lines, and therapeutic uses of the cell lines and resulting vaccines.

The present invention provides a compound exhibiting coronavirus 3CL protease inhibitory activity or a pharmaceutically acceptable salt thereof, and a pharmaceutical composition comprising the same. Provided is a compound represented by Formula (I): wherein Ring A is a ring represented by: wherein X is a single bond or the like, R2 is substituted or unsubstituted aromatic carbocyclyl or the like, R3c is substituted or unsubstituted aromatic carbocyclyl or the like, R3 is substituted or unsubstituted aromatic carbocyclyl or the like, R3a is a hydrogen atom or the like, R3b is a hydrogen atom, R8a is substituted or unsubstituted aromatic carbocyclyl or the like, and R8b is a hydrogen atom or the like, R1 is a substituted or unsubstituted aromatic heterocyclyl, m is 0 or the like, R5a is each independently a hydrogen atom or the like, R5b is each independently a hydrogen atom or the like, R6 is cyano or the like, and R7a and R7b are each independently a hydrogen atom or the like, or a pharmaceutically acceptable salt of the compound.

The application discloses the technical field of biology, and particularly discloses application of moxidectin in preparation of a medicine for resisting akawa virus (AKAV) and a preparation method of the medicine.

The present invention addresses the problem of providing a cultured cell line that has a constant susceptibility to infection by human sapovirus as well as providing measures for establishing said cultured cell line, and of contributing to research on human sapovirus infections and to the development of therapeutic and preventive measures; the inventors of the present invention achieved the present invention after finding that, by performing transformation using a Cas9 gene on Caco-2, which is an easily obtainable commercial cell line derived from human colon cancer, it is possible to acquire, as clones, cells having constant susceptibility to infection by human sapovirus, that a dramatically improved propagation efficiency of human sapovirus is observed in this human sapovirus infection-susceptible cell line, and that propagation by using a subculture is also possible.

According to the negative oxygen antibacterial air pipe for air purification and the preparation method thereof, the inner surface layer of the air pipe is coated with a special modified coating prepared from rare earth, natural mineral powder, metal ions, fluorocarbon resin, a curing agent and a modified rare earth material to form a special modified coating; crystal components in the special modified coating have a piezoelectric effect and a pyroelectric effect under the air pressure condition, potential difference is generated on the surface of the special modified coating material through pressure and temperature changes in an air pipe, so that surrounding air is ionized, ionized electrons act on adjacent water and oxygen molecules and convert the water and oxygen molecules into negative ions, and the negative ions are converted into negative ions. Negative oxygen ions are fed into the indoor environment along with the air inlet system, and human comfort is improved; meanwhile, the generation of potential difference can effectively destroy the growth and reproduction environment of bacteria and viruses, so that the antibacterial and bacteriostatic effects are achieved, and the harm of bacteria, viruses and the like carried in the air to a human body is reduced.

The present invention relates to the use of compositions containing an effective amount of peroxyacid to reduce the viral load in animal feed and water supply, as well as the surfaces and containers in which the feed and water come into contact. Another aspect of the present invention relates to a formaldehyde-free antiviral composition suitable for animal feed, water, and associated storage containers, wherein the composition is capable of controlling the growth of viruses, including but not limited to African Swine Fever, PED, and Avian Influenza. Another aspect of the present invention relates to a kit for treating animal feed to control the growth of viruses comprising a peroxypropionic acid concentrate and a buffered solution.

This invention discloses the application of plant extracts in the preparation of drugs against canine coronavirus, belonging to the field of biomedical technology. The invention found that plant extracts—shikimic acid, phlorizin, caffeic acid, tribulus terrestris saponins, berberine hydrochloride, and apple polyphenols—possess anti-canine coronavirus activity, with phlorizin showing the most significant effect. Phlorizin exhibits antiviral activity in three aspects: direct killing, inhibition of viral adsorption, and inhibition of viral growth, with the inhibition of viral growth being the most significant. Phlorizin acts on the EC50 cells of cCoV. 50 The concentration was 0.616 mg / L. The plant extract of this invention is derived from natural plants and, compared to chemical drugs, has the advantages of low toxicity and good biocompatibility. This invention provides technical support for the development of novel, effective, safe, and side-effect-free pharmaceutical formulations for the prevention and treatment of canine coronavirus.

The present application relates to an avian cell line capable of supporting viral growth of Marek's Disease Virus (MDV), including Herpes Virus of Turkeys (HVT), methods of producing such cell lines, and therapeutic uses of the cell lines and resulting vaccines.

The invention belongs to the technical field of vaccine production, and particularly relates to a rabies virus G protein gene specificity real-time fluorescent quantitative PCR detection primer probe set, a detection kit and a detection method. The primer probe group comprises a pair of primers designed by specific sequences of rabies virus G protein and a corresponding probe, and also comprises a qPCR detection method constructed based on the primer probe combination. According to the method, the copy number of the G protein gene in the virus harvesting liquid in the rabies vaccine production process can be quantitatively detected, so that the dynamic state of virus proliferation is accurately monitored, a virus growth curve is clearly reflected, and rapid and accurate detection of the rabies virus is realized. Compared with a traditional ELISA method, the method has the advantages of being low in cost, high in sensitivity, high in specificity, easy and convenient to operate and the like, and the vaccine production efficiency is effectively improved; in addition, the method can provide reliable data support for optimization of key parameters (such as optimal harvesting time of virus liquid) of a vaccine production process.

The present invention provides a method of inhibiting the growth of proliferating cells, viruses or bacteria in living tissue, the method comprising: applying low to mid-level frequency Alternating Current electromagnetic signals to the living tissue with a transducer comprising a magnetically conductive material passing through a conduction ring energized by an electrical signal to create the low to mid-level frequency AC current electromagnetic signals within the living tissue; wherein the low to mid-level frequency AC current electromagnetic signals have a frequency in the range of about 50 kHz to about 300 kHz and are produced with an AC voltage generator; and circulating fluid in the living tissue provide a secondary coil for the transmission of the low to mid-level frequency AC current electromagnetic signals.

The invention discloses an application of an antiviral compound in prevention and treatment of lichen on lithiasis. The invention belongs to the technical field of biology, and particularly relates to application of an antiviral compound to prevention and treatment of lichen on lithiasis. The antiviral compound disclosed by the invention is oseltamivir and can be applied to prevention and treatment of lichenivirus infection; preparing a product for preventing and treating the lichenivirus infection disease; the growth of lichen viruses is inhibited; preparing a product for inhibiting the growth of the lichen viroid; the lichen activity is inhibited. Oseltamivir is sprayed on the lichens, and the use concentration of the lichens for preventing and treating the lichenoid virus infection is 5mu g / 4cm < 2 >.

The invention belongs to the technical field of biology, and particularly discloses application of moxidectin in preparation of drugs for resisting akabane viruses, it is found that the moxidectin is free of cytotoxicity on MDBK cells by determining the cytotoxicity after AKAV infection, it is found that the moxidectin can serve as an AKAV inhibitor through RT-qPCR, WB and IFA, and the application of the moxidectin in preparation of the drugs for resisting the akabane viruses in preparation of the drugs for resisting the akabane viruses in preparation of the drugs for resisting the akabane viruses in preparation of the drugs for resisting the akabane viruses is achieved. According to the present invention, the akabane virus growth can be inhibited and / or the akabane virus GC protein expression quantity can be reduced, the good inhibition effect on the AKAV virus can be provided when the concentration is 1.25-10 [mu] M, and the new candidate drug is provided for the prevention and treatment of the AKAV virus infection.