3 results about "Morpholino Oligonucleotides" patented technology

The utility model relates to a continuous flow preparation device of morpholino oligonucleotide, and belongs to the field of morpholino oligonucleotide preparation devices. A continuous flow preparation device of morpholino oligonucleotide comprises an automatic synthesizer, a heating device and a constant temperature device, a synthesis column is arranged in the automatic synthesizer, a liquid inlet pipe is connected between an inlet of the synthesis column and the automatic synthesizer, and a liquid outlet pipe is connected between an outlet of the synthesis column and the automatic synthesizer; the heating device covers the surface of the composite column; and the constant temperature device covers the outer wall of the liquid outlet pipe. During continuous flow preparation of morpholino oligonucleotides, the coupling reaction efficiency can be effectively improved, and the impurity content can be controlled.

The application discloses a synthesis method of phosphorodiamidate morpholino oligonucleotide, belongs to the technical field of solid-phase synthesis of phosphorodiamidate morpholino oligonucleotide, and specifically relates to the following steps: adding 9-fluorenylmethyloxycarbonyl-piperazine-succinic acid into Wang resin to mix and react to synthesize 9-fluorenylmethyloxycarbonyl-piperazine-succinic acid-Wang resin, then adding phosphoramidite monomers to perform coupling reaction after deprotection treatment, performing cap treatment after the coupling is completed, then repeatedly performing deprotection treatment, coupling reaction and cap treatment until the coupling of all phosphoramidite monomers is completed, then removing the protecting group of the last phosphoramidite monomer, and then performing cleavage treatment and deprotection treatment of a base protecting group to obtain a phosphorodiamidate morpholino oligonucleotide (PMO) reaction solution. The application provides a synthesis method of phosphorodiamidate morpholino oligonucleotide with high coupling efficiency, low impurity level, high yield and high purity.

Use of antisense thiomorpholino oligonucleotides to treat, prevent, or ameliorate the progression of inflammatory skin diseases or conditions such as psoriasis. Specifically, thiomorpholino¬ containing ASOs that target pre-mRNAs associated with inflammatory skin diseases or conditions such as psoriasis.