DNA fluorescence analysis method based on the interaction between hypericin and 1-pyrene butyric acid

A fluorescence analysis and magnetic separation technology, applied in the field of DNA fluorescence analysis, can solve the problems of difficult single nucleotide polymorphism detection, high background noise of electrochemical method, low hybridization efficiency, etc., achieve good linear response, simple method, The effect of short analysis times
CN106680248BInactive Publication Date: 2019-03-05NANJING UNIV OF SCI & TECH
8 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING UNIV OF SCI & TECH
Publication Date
2019-03-05
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a DNA fluorescence analysis method based on pi-pi stacking interaction between hypericin and 1-pyrenebutyric acid. The method comprises the following steps: hybridizing a capture probe morpholino oligonucleotides covalently linked to the surface of a streptavidin magnesphere with a target DNA fragment, connecting 1-pyrenebutyric acid to a DNA skeleton by using the coordination effect of zirconium ions, and introducing hypericin with strong fluorescence by using the pi-pi stacking interaction between hypericin and 1-pyrenebutyric acid. A fluorescence probe is introduced through the coordination effect of transition metal ions and the pi-pi stacking interaction between aromatic rings, so the method is simple, the binding effect of the fluorescence probe is good, and the measured fluorescence intensity is related with the target DNA; and the fluorescence analysis method realizes highly-sensitive and highly-selective detection of the DNA fragment, and can be applied to detection of a DNA sample in the fields of molecular identification, clinic diagnosis, environment monitoring and food safety.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the application field of fluorescence analysis, in particular to a DNA fluorescence analysis method based on π-π stacking between Hyp and PBA. Background technique

[0002] High-sensitivity and high-specificity DNA detection technology has been widely used in disease diagnosis, drug development, food safety, environmental monitoring, etc. In recent years, DNA detection technology tends to be miniaturized and simplified. The improvement of DNA detection technology, the reduction of cost and easier process control are the current development trends. Compared with early DNA detection methods such as colorimetry, gel electrophoresis, and ultraviolet spectroscopy, DNA sensors using fluorescence methods have attracted more and more attention due to their high sensitivity, low cost, and portability.

[0003] Document 1 (Li Hongye, Fan Shuguo, Liu Yule, Chen Gang, Wang Huanyu, DNA detection by improved polyacrylamide gel electrophores...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More