888 results about "Influenzavirus" patented technology

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas-based kit for isothermal rapid visual detection of H1N1 influenza virus and mycoplasma pneumoniae and a detection method thereof. The kit and the detection method provided by the invention can be used for timely, sensitively and specifically detecting a nucleic acid target in a sample. The invention provides a lateral flow analysis (LFA) detection platform of a three-stage amplification system integrating recombinase polymerase amplification (RPA), CRISPR / Cas12a and catalytic hairpin assembly (CHA) circulation, the detection platform is used for sensitively and accurately detecting mycoplasma pneumoniae DNA (MP DNA) and influenza A virus subtype H1N1RNA (H1N1RNA), and compared with other researches based on an LFA-CRISPR / Cas12a system, the detection platform has the advantages that the detection efficiency is greatly improved, and the detection cost is reduced. According to the kit and the detection method provided by the invention, nucleic acids of two pathogens can be visually detected on a single LFA at the same time, and the sensitivity is high.

The present disclosure relates generally to multisubunit nucleic acids comprising a plurality of polynucleotide sequences, wherein each polynucleotide sequence of the plurality comprises a target sequence, a linker sequence, and a self-assembling sequence, or a linker sequence, a target sequence, a linker sequence, and a self-assembling sequence, or a combination thereof, wherein each polynucleotide sequence of the plurality is connected to an adjacent polynucleotide sequence of the plurality by a cleavage sequence, and wherein the multisubunit nucleic acid further comprises a signal sequence upstream of one or more of the polynucleotide sequences of the plurality, wherein the target sequence is obtained or derived from a respiratory syncytial virus, a metapneumovirus, a human parainfluenza virus, or a combination thereof. The multisubunit nucleic acid encodes a multisubunit peptide.

The invention provides an influenza A / B virus sandwich method detection reagent. The influenza A / B virus sandwich method detection reagent comprises a pair of influenza A virus antibodies and a pair of influenza B virus antibodies, the pair of influenza A virus antibodies comprises a labeled antibody 19C7 and a coated antibody 27H4, the heavy chain amino acid sequence of the 19C7 antibody is SEQ ID NO: 1, and the light chain amino acid sequence of the 19C7 antibody is SEQ ID NO: 2; the heavy chain amino acid sequence of the 27H4 antibody is SEQ ID NO: 9, and the light chain amino acid sequence of the 27H4 antibody is SEQ ID NO: 10; the pair of influenza B virus antibodies comprises a labeled antibody 10G11 and a coated antibody 23E2, the heavy chain amino acid sequence of the 10G11 antibody is SEQ ID NO: 17, and the light chain amino acid sequence of the 10G11 antibody is SEQ ID NO: 18; the heavy chain amino acid sequence of the 23E2 antibody is SEQ ID NO: 25, and the light chain amino acid sequence of the 23E2 antibody is SEQ ID NO: 26. The sandwich method detection reagent has extremely high specificity and sensitivity.

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting canine parainfluenza virus NP protein and application thereof. The combination is composed of monoclonal antibodies 5H10 and 1H7, 5H10 is used as a coating antibody, and 1H7 is used for colloidal gold labeling, so that efficient and specific sandwich detection of CPIV / NP antigen can be realized. The variable region CDR sequences of the heavy chains and light chains of the two antibodies are clear (SEQ ID NO. 1-12). The application also provides a rapid detection test strip based on the antibody combination, which can be used for on-site screening, early diagnosis and epidemiological monitoring of canine parainfluenza virus, solves the problem that existing detection technologies lack high-specificity recognition tools, and has a good application prospect.

The invention belongs to the technical field of biological pharmacy, and particularly relates to influenza virus-like particles for chimeric expression of new coronavirus RBD protein as well as a preparation method and application of the influenza virus-like particles. The virus-like particle is prepared by embedding fusion protein on the surface of influenza virus M1, and the fusion protein comprises an HA2 segment of HA protein of influenza virus, an extracellular functional region of M2e matrix protein, a conserved region short peptide of nucleoprotein, and RBD tandem protein with mutated five sites of 417, 452, 478, 484 and 501 of new coronavirus; the virus-like particles can be used for preventing specific influenza and new crowns; the preparation method of the virus-like particles is simple and low in cost, the virus-like particles are good in immunogenicity and cross protectiveness, the preparation requirements of large-scale influenza vaccines can be met, the biological safety is high, and the immunity of an organism to various influenza viruses and new coronal viruses can be stimulated.

The application provides a virus combined detection immunochromatography test paper, a detection pen and a detection product, and relates to the technical field of immunochromatography test paper. The virus combined detection immunochromatography test paper can detect the novel coronavirus, the influenza A virus and the influenza B virus in a three-in-one manner. The sample binding pad of the immunochromatography test paper is coated with labeled antibodies, including a first influenza A virus antibody, a first influenza B virus antibody, a first novel coronavirus antibody and a first human internal reference protein antibody, and each labeled antibody is labeled with a different color of label. The immunochromatography test paper adopts a double-antibody sandwich principle, and the second influenza A virus antibody, the second influenza B virus antibody and the second novel coronavirus antibody are coated on different detection lines in the detection area. The immunochromatography test paper and the detection pen solve the problems of complex operation and difficult interpretation of the three-in-one detection product of the novel coronavirus, the influenza A virus and the influenza B virus in the prior art.

The invention discloses a multi-subtype influenza A virus nucleic acid detection kit and a detection method, and relates to the technical field of medical detection.The kit comprises a group of reverse transcription RT-RPA amplification primer pairs used for amplifying influenza A virus M gene target sequences, the reverse transcription RT-RPA amplification primer pairs are designed for specific areas of subtypes H1N1, H2N2, H3N2, H5N1 and H7N9, and the reverse transcription RT-RPA amplification primer pairs are designed for specific areas of subtypes H1N1, H2N2, H3N2, H5N1 and H7N9; the crRNA library comprises a plurality of crRNA molecules which are respectively complementary with different conserved regions in the M gene target sequence and is used for guiding Cas12a protein to carry out specific recognition; a Cas12a protein reagent; the reporter molecule can be subjected to non-specific cleavage by the Cas12a protein; the enzyme preparation, the buffer solution and the nucleotide substrate are required by RT-RPA reaction. The invention aims to realize synchronous, rapid, high-sensitivity and high-specificity detection of various subtype influenza A viruses.

The invention provides a bovine parainfluenza virus type 3 QL3 strain, and the preservation number of the bovine parainfluenza virus type 3 QL3 strain is CGMCC No.46469. The invention also provides a bovine parainfluenza inactivated vaccine containing the bovine parainfluenza virus type 3 QL3 strain and purified F protein and HN protein of the QL3 strain. The novel bovine parainfluenza virus type 3 inactivated vaccine is obtained by optimizing the antigen components of the bovine parainfluenza virus type 3 inactivated vaccine, and high-level neutralizing antibodies can be generated after immunizing cattle; the safety of the vaccine is improved through process optimization. Safety and potency test results show that after the inactivated vaccine is used for immunizing cattle, no adverse reaction exists, and high-level neutralizing antibodies are generated. Results show that the immune effect of the bovine parainfluenza virus type 3 inactivated vaccine can be improved.

The invention belongs to the field of biological medicine industry, and discloses a blood marker for assisting in diagnosing influenza. The inventor verifies that the LRG1 is a peripheral blood marker of influenza for the first time and can be used for auxiliary diagnosis of influenza and increase of the detection rate of influenza virus by being matched with multiple times of detection of influenza virus. A test result shows that the peripheral blood LRG1 content 5ng / ml is a CUTOFF value, 46 patients and 4 false negative patients are successfully detected for 50 influenza patients, and the sensitivity is 92%; the number of normal persons is 60, the number of the normal persons is 12, and the specificity is 80%. After influenza treatment, LRG1 and Plt are obviously reduced compared with those before influenza treatment; 0.001, which is equivalent to the level of normal people, Pgt; and 0.05%.

The present application provides chimeric proteins comprising an antibody moiety that specifically binds to a component of an influenza virus or a variant thereof, and a positively charged mucoadhesive peptide fragment. Compositions comprising the chimeric proteins described herein are useful for preventing or treating an infection caused by an influenza virus or a variant thereof in an individual.

The present invention uses ex vivo human airway cultures to assess the human transmissibility and replication competence of influenza and coronavirus strains. By comparing pandemic influenza A subtype H1N1 and highly pathogenic avian influenza H5N1 as reference strains, the transmissibility risk of various viruses was evaluated and categorized. Additionally, an in vitro model evaluated virus-induced impairment of alveolar fluid clearance (AFC) as an indicator of disease severity. The study revealed correlations between bronchus viral replication, human transmission, AFC impairment, and clinical disease severity across different influenza and coronavirus strains.

Provided are an anti-neuraminidase human monoclonal antibody obtained by means of multiple rounds of in-vitro screening and recombinant modification, and the use thereof in the preparation of a product for preventing, detecting, or treating influenza viruses. The provided monoclonal antibody exhibits significant neuraminidase inhibitory activity and broad-spectrum binding capacity to influenza virus antigens, shows excellent preventive and therapeutic effects against multiple seasonal influenza viruses, provides a new technical option for resisting influenza infection, and has great application value.

The invention discloses macromolecular antibacterial artificial leather and a preparation method thereof, and relates to the technical field of artificial leather preparation. The preparation method comprises the following steps: preparing zinc-loaded silicon dioxide with amino grafted on the surface and quaternized polyether polyurethane containing an epoxy group, mixing the zinc-loaded silicon dioxide and the quaternized polyether polyurethane to prepare antibacterial protection slurry, and bonding the zinc-loaded silicon dioxide and the quaternized polyether polyurethane through ring-opening reaction of the amino and the epoxy group during curing to form a cross-linked network, so as to obtain the antibacterial protection layer with antibacterial property, flexibility and wear resistance; zinc ions have an obvious inhibiting effect on bacteria such as gram-positive bacteria and gram-negative bacteria and fungi such as candida albicans, quaternary ammonium salt has an obvious inhibiting effect on influenza viruses, and the zinc ions and the quaternary ammonium salt have a synergistic effect, so that the antibacterial range of the artificial leather is wider, the inhibiting force on germs is stronger, and the antibacterial property of the artificial leather is further improved.

The present invention provides engineered soluble F proteins of paramyxoviruses, such as respiratory syncytial virus (RSV), human metapneumovirus (hMPV), and human parainfluenza virus (hPIV). These engineered proteins are stabilized by specific modifications in the wild-type soluble F sequence, such as substitutions in the 023 chain and / or the introduction of engineered disulfide bonds in the 0 hairpin in the F1 subunit. The present invention also provides a nanoparticle vaccine comprising an engineered soluble F immunogen displayed on a self-assembled nanoparticle. The invention also provides methods of using such vaccine compositions in a variety of therapeutic applications, e.g., methods for preventing or treating viral infections, such as RSV, MPV, and PIV infections.

The invention provides an influenza vaccine adjuvant based on lentinan as well as a preparation method and application thereof. The active ingredient of the vaccine adjuvant is lentinan, the lentinan is extracted from fresh lentinus edodes through a water extraction and alcohol precipitation method, no additional chemical modification is needed, the vaccine adjuvant can be used for preparing the influenza virus vaccine, and the influenza virus vaccine at least contains one of three inactivated influenza virus antigens, namely H1N1 A, H3N2 A and Victoria B. The invention also provides a vaccine preparation and a preparation method thereof, the vaccine preparation comprises the vaccine adjuvant and an influenza virus vaccine, the content of lentinan in the vaccine preparation is 4 mg / mL, and the vaccine preparation has an immunological enhancement effect.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention discloses application of a broad-spectrum influenza virus polypeptide vaccine in preparation of a medicine for preventing influenza virus infection and controlling influenza virus reinfection, and belongs to the technical field of biological medicine. The vaccine is designed on the basis of artificially synthesized polypeptide, a polypeptide sequence which is highly conservative and has immunocompetence is screened and optimized aiming at various subtype influenza virus proteins, a broad-spectrum polypeptide vaccine P125-H is constructed, and an Ii-Key technology and an MF59 adjuvant are applied to enhance immune response. The vaccine can effectively induce immune protection against H1N1 and H7N9 influenza A viruses, significantly reduce the virus load, reduce pathological injury to the lung and improve the survival rate. The P125-H vaccine is composed of three optimized polypeptides, has a cross protection effect on various influenza virus subtypes, has the potential of being developed into a broad-spectrum influenza vaccine, and provides a new technical scheme for coping with influenza virus variation and improving vaccine efficacy.

The invention discloses a primer probe combination and a kit for on-site detection of H5, H7 and H9 subtype avian influenza viruses, and relates to the technical field of gene detection. The invention provides a primer probe combination for detecting H5, H7 and H9 subtype avian influenza viruses on site. The primer probe combination comprises a primer pair and a probe for detecting the H5 subtype avian influenza viruses, the primer pair and the probe are used for detecting the H7 subtype avian influenza virus; the invention discloses a primer pair and a probe for detecting an H9 subtype avian influenza virus. The method for detecting the H5, H7 and H9 subtype avian influenza viruses by adopting the primer probe combination and the kit disclosed by the invention integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has better sensitivity and accuracy, is simple, convenient, rapid and time-saving to operate, and plays an important role in on-site rapid detection of research and safety management of the H5, H7 and H9 subtype avian influenza viruses.

The invention relates to the technical field of biology, and discloses sgRNA for knocking out a TNIP2 gene, the nucleotide sequence is shown as SEQ ID NO: 1, blocking of the sgRNA to TNIP2 expression is verified through sanger sequencing and Western Blotting experiments, a TNIP2 knockout cell line and a control cell have no obvious morphological and growth difference, and the sgRNA can be used for knockout of the TNIP2 gene. The sgRNA can inhibit the replication ability of the H1 subtype influenza virus by regulating and controlling the expression of a host gene TNIP2, in addition, the invention further discloses application of the sgRNA, a cell strain with the TNIP2 gene knocked out and a construction method of the cell strain.

This application relates to the field of virus detection technology, specifically disclosing a real-time fluorescent PCR detection kit and method for H10N7 avian influenza virus. The kit includes sample lysis extract, sample washing solution I, sample washing solution II, and reaction premix for detecting H10N7 subtype avian influenza virus; the detection method is an integrated detection method. This application achieves simultaneous detection of H10 and N7 subtype AIVs by designing specific primers and probes. The kit prepared using these primers and probes not only improves detection efficiency but also significantly enhances sensitivity and specificity. The combined use of MAOPA technology and real-time quantitative PCR technology further improves the accuracy of the detection results, making this kit of this application of significant application value in avian influenza prevention and control.

The invention discloses a primer and probe combination and method for detecting the content of vRNA, cRNA and mRNA of influenza viruses H7N9 in vivo and application of the primer and probe combination and method, and belongs to the technical field of biology. In order to solve the technical problem that the prior art can only detect vRNA, cRNA and mRNA of influenza viruses on in-vitro cell lines and cannot adapt to animal in-vivo infection experiments with complex template quantities, the invention designs a primer and probe combination for detecting in-vivo H7N9 influenza virus PB2 or NA gene vRNA, cRNA and mRNA. The primer and probe combination comprises a reverse transcription primer, a fluorescent quantitative PCR primer and a probe, the obtained primer and probe combination and a fluorescent quantitative PCR technology are utilized to measure the vRNA content, the cRNA content and the mRNA content of PB2 and NA genes of the H7N9 virus in a mouse body, and an experimental scheme is provided for researching the real copying condition of the influenza virus H7N9 in the body.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting canine parainfluenza virus NP protein and application of the monoclonal antibody combination. The combination is composed of monoclonal antibodies 5H10 and 1H7, the 5H10 serves as a coating antibody, the 1H7 is used for colloidal gold labeling, and efficient and specific sandwich detection of CPIV / NP antigens can be achieved. The heavy chain and light chain variable region CDR sequences of the two antibodies are clear (SEQ ID NO. 1-12). The invention further provides a rapid detection test strip based on the antibody combination, the rapid detection test strip can be used for on-site screening, early diagnosis and epidemiological monitoring of the canine parainfluenza virus, the problem that an existing detection technology is lack of a high-specificity recognition tool is solved, and the rapid detection test strip has a good application prospect.

This article provides a combination of compounds that can inhibit influenza virus replication, reduce influenza virus load, and / or treat influenza.

The present disclosure is directed to peptide antigens derived from a previously undefined epitope on influenza A virus hemagglutinin and methods for use thereof.

The invention discloses a primer composition for respiratory tract pathogen detection, a related product and application, and belongs to the field of pathogen detection. The primer composition comprises a primer pair aiming at influenza A virus, a primer pair aiming at influenza B virus, a primer pair aiming at bordetella pertussis, a primer pair aiming at adenovirus, a primer pair aiming at respiratory syncytial virus and a primer pair aiming at mycoplasma pneumoniae. The primer composition and related products can be used for simultaneously detecting influenza A viruses (seasonal H1N1, novel influenza A H1N1 viruses (2009), H3N2, H5N1 and H7N9), influenza B viruses (Victoria series and Yamagata series), adenoviruses (group B, group C and group E), respiratory syncytial viruses (subtype A and subtype B), mycoplasma pneumoniae and bordetella pertussis, so that pathogenic pathogens are quickly determined, and the primer composition and related products have important significance on clinical detection.

The invention discloses an influenza virus rapid preliminary screening detection method, and relates to the technical field of biological detection, and the method comprises the following steps: collecting fluorescence signal data of a detected sample, constructing a fluorescence signal time curve, dividing the fluorescence signal time curve into a baseline segment, an amplification segment and a platform segment, and adopting a weighted sliding difference curvature method to detect the influenza virus. Calculating a local curvature change rate of each center time point of an amplification section, constructing a dynamic curvature judgment reference based on noise characteristic parameters, further obtaining an amplification trend judgment result Boolean value, and generating a final preliminary screening conclusion by calculating a fluorescence fluctuation proportion and a fluorescence slope mean value of fluorescence signal data and judging an amplification termination behavior state. According to the method, the dynamic curvature judgment reference is constructed based on the baseline segment signal variance value and the maximum jump amplitude value, so that the curvature judgment threshold value can be flexibly adjusted along with the background noise level, misjudgment caused by fixed parameters is avoided, and stable amplification trend judgment under various noise conditions is realized.

The application discloses a method for producing pectin oligosaccharides by microbial degradation of pomace and application thereof. The method comprises the following steps: adding microbacterium A5 to a culture medium containing pomace to perform fermentation to obtain a fermentation liquor, performing fractional pressing filtration and filter sterilization on the fermentation liquor, performing ethanol precipitation to extract a pectin crude product, removing protein, performing ion exchange chromatography, dialysis desalting, freeze drying and the like to purify two pectin oligosaccharides POS-1 and POS-2 with a purity greater than 95%; the pectin oligosaccharides POS-1 and POS-2 have strong scavenging capacity on hydroxyl radicals and DPPH free radicals and the capacity of inhibiting the activity of respiratory RNA viruses such as influenza virus H1N1 and respiratory syncytial virus RSV, and can be applied to medicines or health foods.

The present invention pertains to the use of a vaccine based on an alphavirus RNA replicon particle (αRP) vector encoding an antigen of an IAV-S for the passive vaccination of piglets against a pathogenic infection with swine influenza virus.

The invention relates to the field of ultraviolet sterilization systems, in particular to an integrated ultraviolet sterilization system utilizing a UVC deep ultraviolet chip, which comprises a UVC deep ultraviolet chip lamp bead module serving as a core light source to emit deep ultraviolet light for sterilization; the self-adaptive heat dissipation module is attached to the UVC deep ultraviolet chip lamp bead module through heat conduction silicone grease and used for dynamically guiding out heat of the UVC deep ultraviolet chip lamp bead module; and the optical power closed-loop driving module is electrically connected with the UVC deep ultraviolet chip lamp bead module and is used for outputting stable current and performing feedback regulation according to optical power. According to the integrated ultraviolet sterilization system using the UVC deep ultraviolet chip, the high efficiency is remarkably improved: the NPSS + superlattice insertion layer enables the electro-optical conversion efficiency to be improved to more than 8%, and the deep ultraviolet light of 265-275 nm is 1t; the inactivation rate of escherichia coli and influenza viruses within 1 minute reaches 99.99%, and the disinfection and killing efficiency is improved by 30% compared with that of existing equipment.