37 results about "Virus gene expression" patented technology

The present invention provides siRNA molecules that target influenza virus gene expression and methods of using such siRNA molecules to silence influenza virus gene expression. The present invention also provides nucleic acid-lipid particles that target influenza virus gene expression comprising an siRNA that silences influenza virus gene expression, a cationic lipid, and a non-cationic lipid.

The invention relates to RNA (Ribonucleic Acid) interference carriers of RSV (Rice Stripe Virus) and RBSDV (Rice Black-Streaked Dwarf Virus) as well as a construction method and application thereof, belonging to the technical field of biology. The invention establishes RNA interference carriers pMCG161+ / -D and pCMBIA1301+ / -D of the RSV by using sequences shown as SEQ ID NO1. The invention provides an interference virus gene expression carrier for plant disease-resistant gene engineering as well as a construction method and application thereof and provides a new concept for the application of a transgenic technology and RNA interference in biological resistant breeding. By transplanting the carrier to rice, a virus-resistant plant is successfully acquired, the expected target of obtaining a RSV and RBSDV-resistant rice new material according to the principle of RNA interference is realized, a successful example is provided for plant resistant breeding and gene engineering of RNA interference mediums and the blank of researches on the RSV and RBSDV resistance of the RNA interference mediums in the field is made up.

This invention pertains to polynucleotides, such as small interfering RNA (siRNA), useful for reducing the expression of respiratory syncytial virus (RSV) genes within a subject; and methods for treating a patient suffering from, or at risk of developing, an RSV infection by administering such polynucleotides to the subject.

The invention provides siRNA compositions that interfere with viral replication in respiratory viral infections, including respiratory syncytial virus and avian influenza A, including the H5N1 strain. The invention further provides uses of the siRNA compositions to inhibit expression of viral genes in respiratory virus-infected cells, and to uses in the treatment of respiratory virus infections in a subject. Generally the invention provides polynucleotide that includes a first nucleotide sequence of 15 to 30 bases that targets the genome of a respiratory syncytial virus or an influenza A virus, a complement thereof, a double stranded polynucleotide or a hairpin polynucleotide. Additionally the invention provides vectors, cells and pharmaceutical compositions containing siRNA sequences.

The present invention provides novel expression vectors which permit tight regulation of gene expression in eucaryotic cells. More specifically, the invention provides DNA vectors comprising nucleotide sequences that are transcribed to form RNA molecules which are then replicated by a temperature-sensitive replicase to form additional RNA molecules. The RNA molecules produced by replication contain a nucleotide sequence which may be translated to produce a protein of interest or which encode one or more untranslated RNA molecules. Also provided are methods for producing heterologous proteins and untranslated RNA molecules. Further provided are methods for administering heterologous proteins and untranslated RNA molecules to individuals. In addition, pharmaceutical compositions are provided comprising the DNA and RNA molecules of the invention and a pharmaceutically acceptable carrier.

The present invention provides virus vectors of the family Paramyxoviridae in which the transcription start (S) sequence has been modified so as to modify the expression of genes located downstream thereof, a method for producing the vectors, and uses thereof. By measuring the transcription initiation efficiency of the S sequence of each gene carried by Sendai viruses (SeV), it was clarified that the S sequence of F gene has a significantly lower ability to promote transcription than the other three S sequences. When the S sequence of the F gene of wild type Sendai virus was substituted by the S sequence of the P / M / HN gene-type showing a high transcription initiation efficiency, the F gene of the resultant Sendai virus mutant and genes located downstream thereof show elevated expression levels. It was also revealed that this mutant proliferates more quickly than the wild type. The vectors of this invention are useful in elevating the expression of foreign genes and producing pharmaceutical compositions and vaccines. Furthermore, by lowering virus gene expression from virus vectors, it is possible to suppress transcription and / or replication and reduce cytotoxicity of the vector genome.

We disclose a polypeptide capable of binding to a nucleic acid comprising a viral nucleotide sequence. Preferably, the viral nucleotide sequence comprises a viral promoter sequence, for example, an HIV promoter or a herpesvirus promoter sequence.

The invention relates to 'an expression vector of interfering viruses of barley yellow dwart viruses, a constructing method, and the application' and belongs to the technical field of biological engineering. The invention provides the expressing vector of interfering viruses of barley yellow dwart viruses which is characterized in that a skeleton carrier which is adopted is pMCG161, a sense strand of a coat protein gene of the barley yellow dwart viruses is inserted between two restriction sites of AscI and AvrII which are on the upstream portion of the pMCG161, and an antisense strand of the coat protein gene of the barley yellow dwart viruses is inserted between two restriction sites of SpeI and SgfI which are on the downstream portion of the pMCG161. The expression vector of interfering viruses of barley yellow dwart viruses GAV which is constructed by the invention is transferred into wheat through particle bombardment to obtain a transgene wheat variety which has resistance to the barley yellow dwart viruses GAV. The invention provides a breeding way with high efficiency and a new strategy.

A method for quantifying infectious particles of a virus in a sample comprises providing a layer of host cells of the virus, contacting the layer of host cells with a preparation of the sample, and culturing the cells under conditions wherein the cells are submerged in a thin layer of liquid culture medium, and wherein the virus infects host cells and releases its progeny from said infected host cells, imposing a flow of the liquid medium, wherein the spread of the viral progeny to uninfected host cells is enhanced, culturing the cells under conditions to allow further virus infection and viral gene expression, wherein infected host cells develop an observable indication of viral gene expression, and determining the number of infected host cells, whereby the number of infectious particles of the virus in the sample is quantified. The method may be used for measuring viral growth rate or for screening for antiviral compounds. Also provided are microfluidic devices suitable for the inventive method.

A method for enhancing the serum stability and lowering the immunostimulatory property of a small interfering ribonucleic acid (siRNA) which mediates RNA interference (RNAi) against a viral gene expression of hepatitis B virus (HBV) or hepatitis C virus (HCV) is provided.

The invention provides a method allowing the detection and the quantitative real-time measurement, at the single living cell level, of viral replication using a bioluminescent reporter gene and a digital light detection device sensitive to detect single photons with high efficiency and assign them a lateral x,y coordinate and precise temporal incidence (i.e. a “time point”), wherein the spatial and temporal characteristics of bioluminescence is indicative at the single living cell level of viral replication.

The invention provides a method for obtaining polyhedron by recombining baculovirus and utilizing the obtained polyhedron to feed, wherein, silkworm baculovirus polyhedron genes are transposed onto chromosomes of silkworm cells; recombined silkworm baculoviruses are infected the silkworm cells after modification; the polyhedron genes positioned on the chromosomes of the silkworm cells express along with stimulus of expression of virogenes, thereby the recombined baculoviruses can be embedded into the polyhedron; the polyhedron is then sprayed on mulberry leaves, and silkworm larvae can be infected after eating the mulberry leaves, thereby work efficiency is greatly improved.

The invention provides siRNA compositions that interfere with viral replication in respiratory viral infections, including respiratory syncytial virus and avian influenza A, including the H5N1 strain. The invention further provides uses of the siRNA compositions to inhibit expression of viral genes in respiratory virus-infected cells, and to uses in the treatment of respiratory virus infections in a subject. Generally the invention provides polynucleotide that includes a first nucleotide sequence of 15 to 30 bases that targets the genome of a respiratory syncytial virus or an influenza A virus, a complement thereof, a double stranded polynucleotide or a hairpin polynucleotide. Additionally the invention provides vectors, cells and pharmaceutical compositions containing siRNA sequences.

The invention relates to a rice stripe virus RNA interference vector, a constructing method and application thereof, belonging to the technical field of biology. The method establishes the rice stripe virus RNA interference vector pMCG161+ / -R and pCMBIA1301+ / -R by using a sequence shown in SEQ ID NO 3 as sense and antisense strands. The invention provides the vector interfering with virus gene expression and the constructing method and application thereof for genetic engineering of plant disease resistance, and provides a new idea for transgenic technology and application of RNA interference in biological resistance breeding. The vector is transferred into paddy to obtain antivirus plant successfully, the preset target of obtaining the anti RSV paddy new material by using RNA interference principle can be realized, a successful case is provided for RNA interference mediated plant resistance breeding and genetic engineering, and the blank of RNA interference mediated RSV resistance in the research of the field can be filled up.

This invention relates to modified short interfering RNA (siRNA) nucleic acid molecules, particularly siRNA's which have been modified by the addition of a 2 -0- guanidinopropyl (GP) modified nucleoside. In particular the invention relates to modified siRNAs which are capable of silencing target sequences, methods of treating and preventing infection by using the siRNAs, medicaments containing the siRNAs and use of the siRNAs.

The invention discloses a VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus. A Tiantan strain oncolytic vaccinia virus VGF gene is removed, an EGFP gene is expressed, and the base sequence of the VGF gene is shown as a sequence table 1. A preparation method and applications of the VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus are also disclosed. Through the VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus VTT-VGFE, the toxicity of the Tiantan strain oncolytic vaccinia virus can be reduced, and the tumor selectivity of the Tiantan strain oncolytic vaccinia virus can be enhanced; and the VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus VTT-VGFE has a function of monitoring the distribution of virions in vivo inreal time, provides a good tool for researching and developing live vaccines and tumor biotherapy by taking vaccinia virus as a carrier, and can be suitable for treating lung cancer, liver cancer, melanoma and other various tumors.

The invention provides a duck circovirus tandem repeated sequence and application thereof and belongs to the technical fields of bioengineering and molecular biology. The invention firstly discovers aQTR sequence in which DuCV specifically exists, and the tandem repeated sequence does not appear in other circoviruses. QTR can serve as a gene I type and II type DuCV genetic typing new molecular marker; meanwhile, the invention proves the effect of regulating and controlling mRNA stability by the QTR serving as a DSE component by a dual-luciferase reporter system and a semi-anchored reverse mutation PCR technology, and defines the important effect of regulating and controlling virus gene expression by the QTR. The invention provides important technical and theoretical basis for researchingDuCV genotyping and virus replication regulation and control mechanism.

Genetically modified CCNT1 and XPO1 genes encoding proteins that inhibit virus infection in cells. The genetically modified CCNT1 gene encodes a protein with a C261Y substitution with respect to the human CCNT1 protein. The genetically modified XPO1 gene encodes a protein with P411T, M412V, and / or F414S substitutions with respect to the human XPO1 protein. The genetically modified CCNT1 and XPO1 genes can be introduced in cells. The cells comprising the genetically modified CCNT1 and XPO1 genes can be introduced in a subject with a virus infection to treat the infection.

This invention pertains to polynucleotides, such as small interfering RNA (siRNA), useful for reducing the expression of respiratory syncytial virus (RSV) genes within a subject; and methods for treating a patient suffering from, or at risk of developing, an RSV infection by administering such polynucleotides to the subject.

The invention relates to the technical field of biological detection, in particular to a fluorescent quantitative PCR method for detecting a potato leaf virus. The fluorescent quantitative PCR methodcomprises the following steps that 1, RNA extraction is performed, wherein the total RNA of potatoes is extracted; 2, reverse transcription is performed, the extracted total RNA is reversely transcribed into cDNA; 3, primer design is performed, wherein two pairs of primers for amplification of an internal reference gene and a PLRV gene are respectively designed; 4, fluorescent quantitative PCR isperformed, the reversely transcribed cDNA and the designed primers are prepared into a PCR reaction system, and real-time fluorescent quantitative PCR reaction is performed. By adopting the detectionmethod, it can be detected at the molecular level that the potato leaf virus exists in potato plants, the virus gene expression situation can be quantitatively analyzed, and the fluorescent quantitative PCR method has the advantages of high efficiency, strong specificity, good accuracy and repeatability, high sensitivity, high detection speed, low material consumption, low cost, quantitative detection and the like.

The invention relates to a rice stripe virus RNA interference vector, a constructing method and application thereof, belonging to the technical field of biology. The method establishes the rice stripe virus RNA interference vector pMCG161+ / -R and pCMBIA1301+ / -R by using a sequence shown in SEQ ID NO 3 as sense and antisense strands. The invention provides the vector interfering with virus gene expression and the constructing method and application thereof for genetic engineering of plant disease resistance, and provides a new idea for transgenic technology and application of RNA interference in biological resistance breeding. The vector is transferred into paddy to obtain antivirus plant successfully, the preset target of obtaining the anti RSV paddy new material by using RNA interference principle can be realized, a successful case is provided for RNA interference mediated plant resistance breeding and genetic engineering, and the blank of RNA interference mediated RSV resistance in the research of the field can be filled up.

A method for quantifying infectious particles of a virus in a sample comprises providing a layer of host cells of the virus, contacting the layer of host cells with a preparation of the sample, and culturing the cells under conditions wherein the cells are submerged in a thin layer of liquid culture medium, and wherein the virus infects host cells and releases its progeny from said infected host cells, imposing a flow of the liquid medium, wherein the spread of the viral progeny to uninfected host cells is enhanced, culturing the cells under conditions to allow further virus infection and viral gene expression, wherein infected host cells develop an observable indication of viral gene expression, and determining the number of infected host cells, whereby the number of infectious particles of the virus in the sample is quantified. The method may be used for measuring viral growth rate or for screening for antiviral compounds. Also provided are microfluidic devices suitable for the inventive method.

This invention describes a novel gene regulatory sequence containing the promoter and enhancer sequences of the human cardiac troponin T gene (TNNT2) that selectively induces expression in cardiomyocytes. This novel TNNT2 promoter / enhancer composition can be used to induce adeno-associated virus gene expression, construct cell-type-specific expression vectors, or perform cardiac-specific transgenesis. The use of this novel promoter / enhancer composition is demonstrated by the expression of mAKAP shRNA and a mAKAP-derived anchor-disrupting peptide useful for the treatment of heart failure.

The present disclosure provides a method of inhibiting telomerase activity to reduce human herpesvirus (e.g., HMCV, EBV, HSV-1, and / or HHV-6 / 7 / 8) replication. The method comprises treating cells with a telomerase inhibitor prior to and after HCMV infection. The telomerase inhibitor may be a pharmaceutical inhibitor such as BIBR1532 or MST312, or an siRNA construct targeting the hTERT catalytic subunit of telomerase. Treatment with the telomerase inhibitor results in reduced viral titer, decreased viral gene expression, and diminished viral protein levels across different temporal phases of herpesvirus infection. The method provides a novel approach for inhibiting herpesvirus replication through targeting of host cell telomerase activity.

The invention provides an HPV (human papillomavirus) infected human epidermal keratinocyte model and a construction method and application thereof, and relates to the technical field of molecular biology, an HPV genome is integrated to a KRT5 site of genome DNA (deoxyribonucleic acid) of human epidermal keratinocytes to obtain the HPV infected human keratinocyte model, stable integration and expression of an HPV whole genome are successfully realized, and the HPV infected human keratinocyte model is obtained. The position effect caused by random insertion is avoided, and the genetic stability is ensured; the model has the synergistic expression function of HPV early and late genes, maintains the time sequence of virus gene expression, realizes HPV virus particle assembly, and simulates the complete life cycle of HPV. The technical problem that in the prior art, an infection model cannot simulate the complete life cycle of the HPV is solved.

The invention provides application of an atractylodin alcohol (CZSC) compound in preparation of medicaments for treating animal virus diseases, in particular application in preparation of a medicament for treating porcine reproductive and respiratory syndrome virus (PRRSV) infection and a medicament for treating porcine reproductive and respiratory syndrome (PRRS), or in preparation of a virus gene expression inhibitor and an anti-virus medicament and in preparation of a medicament for inhibiting blue ear disease viruses. The CZSC has remarkable effects of regulating nucleoprotein gene expression of the PRRSV down and inhibiting virus proliferation; and in vitro cellular pharmacological tests shows that the CZSC has definite anti-virus effect. The CZSC has wide application prospect in treatment and control of PRRSV infection.

The invention discloses application of acridine compounds in preparation of anti-human immunodeficiency virus drugs, and belongs to the field of preparation of antiviral drugs. Experiments find that the compound can significantly improve the latent HIV-1 transcriptional activity in the body of a patient when being used for preparing drugs for treating AIDS, so that the compound can be recognized by anti-retrovirus drugs. Taking HIV-1 latent infection cells as an example, the compound enables the expression quantity of HIV-1 virus genes to be remarkably improved, and has the potential of activating a virus repository. Therefore, the compound has the potential of eliminating the virus latent library in the body of an AIDS patient, and provides a new possibility for functional cure of AIDS.

The invention relates to the technical field of biological detection, in particular to fluorescent quantitative PCR primers and kit for detecting a potato leaf virus. The fluorescent quantitative PCRprimers include primers Actin-F and Actin-R for amplifying reference genes and primers PLRV2-F and PLRV2-R for amplifying PLRV genes. The kit comprises the above primers. The primers and the kit can detect at the molecular level that the potato leaf virus exists in potato plants, the virus gene expression situation can be quantitatively analyzed, and the fluorescent quantitative PCR primers have the advantages of high efficiency, strong specificity, good accuracy and repeatability, high sensitivity, high detection speed, low material consumption, low cost, quantitative detection and the like.

Disclosed herein are small interfering RNA (siRNA) molecules and pharmaceutical compositions containing them for the prevention and treatment of Ebola virus disease. The present invention provides siRNA molecules that inhibit Ebola virus gene expression, compositions containing the molecules, and methods of using the molecules and compositions to prevent or treat EVD in a subject, such as a human patient.

The present invention provides compositions comprising siRNA molecules that target Marburg virus (MARV) gene expression, lipid particles comprising one or more (e.g., a combination) of the siRNA molecules, and methods of delivering and / or administering the lipid particles, for the purposes of treating MARV infection.