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Fluorescent quantitative PCR primer and kit for detecting potato leaf roll virus

A leaf-rolling virus, fluorescence quantitative technology, applied in biochemical equipment and methods, microorganisms, microorganism-based methods, etc., can solve the problems of large consumption of detection materials, quantitative detection of viruses, low sensitivity, etc., and achieve good accuracy. , Good primer specificity, sensitive and accurate detection

Inactive Publication Date: 2018-12-28
HUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with real-time fluorescent quantitative PCR technology, other methods have some shortcomings, such as relatively long time-consuming, large consumption of detection materials, low sensitivity, and inability to quantitatively detect viruses, etc.

Method used

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  • Fluorescent quantitative PCR primer and kit for detecting potato leaf roll virus
  • Fluorescent quantitative PCR primer and kit for detecting potato leaf roll virus
  • Fluorescent quantitative PCR primer and kit for detecting potato leaf roll virus

Examples

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Embodiment Construction

[0022] For the convenience of those skilled in the art to understand, below in conjunction with embodiment and appended Figure 1-6 To further illustrate the present invention, the content mentioned in the embodiment is not to limit the present invention.

[0023] Specific experimental conditions and methods are not indicated in the following examples, usually according to conventional conditions such as: editors such as J. Sambrook, Science Press, 1992, Molecular Cloning Experiment Guide (Second Edition): D.L. Spector, etc., Science Publishing House, 2001, Cell Experiment Guide: Lu Hongsheng, Science Press, 1982, or according to the conditions suggested by the manufacturer.

[0024] Potato leafroll virus-infected plants of the present invention are sampled at the Jiuhua Potato Base in Tiechong Town, Huidong County, Guangdong Province. Symptom observations are carried out on the collected potato virus-infected plants, and whether the potato samples are preliminarily identified...

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Abstract

The invention relates to the technical field of biological detection, in particular to fluorescent quantitative PCR primers and kit for detecting a potato leaf virus. The fluorescent quantitative PCRprimers include primers Actin-F and Actin-R for amplifying reference genes and primers PLRV2-F and PLRV2-R for amplifying PLRV genes. The kit comprises the above primers. The primers and the kit can detect at the molecular level that the potato leaf virus exists in potato plants, the virus gene expression situation can be quantitatively analyzed, and the fluorescent quantitative PCR primers have the advantages of high efficiency, strong specificity, good accuracy and repeatability, high sensitivity, high detection speed, low material consumption, low cost, quantitative detection and the like.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a fluorescent quantitative PCR primer and a kit for detecting potato leafroll virus. Background technique [0002] Potato leafroll virus (PLRV) is classified as Luteovirus group (Luteovirus), is a member of the genus PLRV, and is one of the most important viruses in potato virus diseases that endanger potato production. The PLRV virion is spherical, equiaxed, and 24nm in diameter. It is a positive-strand RNA virus without poly A at the 3' end and a strictly conserved sequence of 5-20 bp at the 5' end. The virus is transmitted in seed potatoes, and is transmitted by aphids in a persistent manner in the field. It cannot be transmitted mechanically. The vascular bundle of the host plant is its main distribution site. The content of the virus in the host body is low, and it is difficult to purify in large quantities, so that people cannot conduct thorough molecular biol...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11C12R1/94
CPCC12Q1/6851C12Q1/701C12Q2600/166C12Q2531/113C12Q2545/101
Inventor 陈兆贵叶新友侯红因邢澍祺毛露甜黄雁
Owner HUIZHOU UNIV
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