Method of detecting nucleotides of Bacillus cereus, primers for detecting and probes

A technology for detecting primers for Bacillus cereus, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve high sensitivity, high accuracy, and good primer specificity
CN103205485BActive Publication Date: 2014-12-03SOUTH CHINA UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA UNIV OF TECH
Publication Date
2014-12-03

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Abstract

The invention discloses a method of detecting nucleotides of Bacillus cereus, primers for detecting and probes, belonging to the technical field of biological detection. According to the invention, the primers designed according to genome sequence of the Bacillus cereus are good in singularity, and high in sensitivity if used for fluorescence PCR detection. The detection method provided by the invention is high in accuracy and sensitivity, can rapidly and simply determine whether samples contain the Bacillus cereus, and provides scientific basis for etiological diagnosis and differential diagnosis of the Bacillus cereus in various dairy products.
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Description

technical field

[0001] The invention belongs to the technical field of biological detection, in particular to a method for detecting nucleotides of bacillus cereus, primers and probes for detection. Background technique

[0002] Bacillus cereus is an aerobic, mesophilic, spore-forming, Gram-positive bacillus widely distributed in soil, water and air dust. There are many types of strains, including probiotic strains, low-toxicity strains and a few high-virulence strains. High-virulence strains often lead to food poisoning outbreaks and are common food-contaminating bacteria. Bacterial cells are rod-shaped, with square ends, in short or long chains, 1.0-1.2ร—3.0-5.0 microns. Produce spores, spores are round or cylindrical, mesozoic or near mesozoic, and cysts have no obvious swelling. Acapsular, motile. The colonies are large, rough, flat, and irregular. Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis form the group of Bacillus cereus. Their morphological cha...

Claims

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