The invention discloses a
cell hologram automatic focusing and aberration compensation
phase reconstruction method based on
deep learning, and the method comprises the steps: obtaining a to-be-processed off-axis digital hologram of a
cell sample based on an off-axis
digital holographic microscopy system, and carrying out the preprocessing of an original hologram, and enhancing the data size;
processing the holograms in batches by using a
phase reconstruction method considering aberration compensation to generate corresponding phase diagrams, and forming a ''hologram-
phase diagram''
image pair data set for model training; the
image pair data set is used for training a pix2pix
network model, the input of the model is a
cell hologram, and the output of the model is a focusing
phase diagram; according to the invention, automatic focusing and
phase reconstruction can be simultaneously realized for a single hologram, a clear and distortionless in-focus
phase diagram is directly output, the problem that only an object in a specific distance range can be clearly imaged is solved, and the method is suitable for cell observation,
label-free
pathological detection and dynamic analysis of living cells in the field of
biomedicine.