The invention provides a co-culture
system for increasing the yield of endometrial
stem cell exosomes and a method for increasing the yield of endometrial
stem cell exosomes, firstly, fibroblasts are subjected to
mitomycin C pretreatment, so that the cells are stagnated in G1, S and G2 stages of a
cell cycle, meanwhile,
RNA and
protein synthesis continues to be carried out, and the endometrial
stem cell exosomes are obtained; the fibroblasts are kept in a non-proliferative but metabolic
active state; then, endometrial stem cells extracted from endometrial tissues in the proliferation period and fibroblasts are indirectly co-cultured through a Transwell chamber and are separated through a semi-permeable membrane, and stem
cell differentiation caused by inter-
cell contact reaction and fusion of the stem cells and somatic cells is eliminated; after co-culture, taking out the endometrial stem cells, putting the endometrial stem cells into a serum-free culture medium special for exosomes for separate culture, harvesting cell supernate, and purifying the exosomes; compared with the endometrial stem cells which are independently cultured in the whole process, the co-culture
system disclosed by the invention has the advantages that the amplification speed of the endometrial stem cells and the
exosome yield of unit quantity of cells are remarkably improved.