4results about How to "Sensitive and accurate detection" patented technology

The invention discloses a multi-gating DNA cascade amplifier and application, and relates to the technical field of clinical medicine, the multi-gating DNA cascade amplifier comprises a DNA fluorescent probe and a manganese dioxide carrier, the DNA fluorescent probe is adsorbed on the manganese dioxide carrier to form a DNA nano fluorescent probe, and the DNA fluorescent probe is hairpin type DNA containing H1, H2, H3, H4 and AH5-FAM; the DNA cascade amplifier can accurately and sensitively detect the PLS3 mRNA level in living cells, and provides a valuable tool for early recognition of small metastases of breast cancer, assessment of metastasis risks and enhancement of personalized medical methods in cancer treatment.

This invention relates to the field of analytical detection technology, and discloses a high-throughput rapid detection platform for contaminants in food, its construction method, and its applications. The high-throughput rapid detection platform is constructed by immobilizing universally applicable biorecognition sensing probes targeting contaminants on a 96-well plate to achieve high-throughput rapid detection. The biorecognition sensing probes include a Pt NP-modified signal transduction probe (Bond) and an arm-containing aptamer that specifically recognizes the contaminants. This invention constructs an aptamer sensor by modifying Pt NPs onto a triple-helix molecular switch signal transduction probe. Simultaneously, utilizing its peroxidase mimic activity and fluorescence quenching capability, the activity of Pt NPs is modulated via the triple-helix molecular switch, developing a universal dual-mode aptamer sensing platform. This platform retains the inherent advantages of each signal mode while supporting real-time cross-validation, helping to reduce systematic errors and suppress external interference, effectively reducing false positive and false negative rates.

This invention belongs to the field of environmental and food safety testing technology, and specifically discloses a label-free Cd based on SYBR Green I dye. 2+ A rapid detection fluorescent aptamer sensor and its quantitative detection kit are designed to achieve highly sensitive and specific quantitative detection of cadmium ions in samples such as fruits, vegetables, and food products. The fluorescent aptamer sensor includes the nucleic acid aptamer Cd-(21) and its complementary strand CS. Cd‑(21) And SYBR Green I dye; this fluorescent aptamer sensor is used to detect Cd. 2+ This invention offers advantages such as simple operation, short processing time, high sensitivity, and good selectivity. It also provides a matching reagent kit containing the necessary aptamers, complementary strands, SGI, buffer solutions, and other reagents, facilitating operation and application. The kit is simple in design, suitable for various laboratory and field testing applications, and has broad application prospects. The label-free fluorescent aptamer sensor and its matching reagent kit of this invention are Cd... 2+ This provides a new solution for efficient detection, with good technical and practical application value.

The probe for the computerized flat knitting machine is an elastic probe, and the elastic structure with the automatic reset function and the buffering function is integrally arranged on the probe. The utility model also relates to a broken needle alarm device, which comprises two probes arranged on the left and right of the machine head through insulating bases, an alarm control assembly, the probe is an elastic probe, the elastic structure with the automatic reset function and the buffering function is integrally arranged on the probe, the upper portion of the probe is connected with a weak current lead, and the machine head moves left and right on the needle plate to realize knitting. When the probe lower portion and the broken needle needle foot falling from the needle plate contact, the circuit of the alarm control assembly is connected to realize the broken needle alarm. The elastic probe can effectively reduce the impact force of the needle foot contact and automatically reset after the broken needle alarm is removed. In addition, the probe is arranged at the inner bottom plate of the machine head of the computerized flat knitting machine and located between the left and right sides of the needle lifting cam and the needle returning cam, so that the detection of the broken needle is more timely and accurate, and the knitting product quality is improved.