Method of detecting content of preservative in cosmetic

A technology for preservatives and cosmetics, applied in the fields of analytical chemistry and daily chemical safety, can solve problems such as toxicity, and achieve the effects of low quantitative concentration, sensitive and accurate detection, and wide linear range.

Inactive Publication Date: 2015-11-11
云南省食品药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical preservatives are the most commonly used in cosmetics. Usually a small amount of preservatives will not irritate the skin, but most types of preservatives are more or less toxic to a certain extent. Some manufacturers use controversial preservatives or add preservatives in excess to extend product shelf life

Method used

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  • Method of detecting content of preservative in cosmetic
  • Method of detecting content of preservative in cosmetic
  • Method of detecting content of preservative in cosmetic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 adopts method of the present invention to detect preservative content in cosmetics

[0036] Instruments: Agilent1260InfinityHPLC high-performance liquid chromatography system with PDA detector (Agilent); electronic balance (MettlerToledoNewClassicMF); ultrasonic cleaner (ElmasonicD-7700); centrifuge (Sigma3k15).

[0037] Standard products: methylisothiazolinone (content 99.0%), 2-bromo-2-nitropropane-1,3-diol (content 99.0%), methylchloroisothiazolinone (content 99.0%), Phenoxyethanol (content 99.5%), methyl 4-hydroxybenzoate (content 99.5%), benzoic acid (content 99.5%), ethyl 4-hydroxybenzoate (content 99.5%), isopropyl 4-hydroxybenzoate Esters (99.8%), Propyl 4-Hydroxybenzoate (99.5%), Isobutyl 4-Hydroxybenzoate (99.7%), Butyl 4-Hydroxybenzoate (99.0%), a total of 11 types , Manufacturers are: Dr. Ehrenstorfer GmbH.

[0038] Cosmetics to be tested: lotion of a certain brand

[0039] Reagents: acetonitrile and methanol are chromatographically pure (DIM...

Embodiment 2

[0057] Day-to-day reproducibility test in embodiment 2

[0058] The method of Example 1 was used for 3 consecutive days and 6 repetitions per day to detect the preservative content in a certain brand of cosmetic emulsion. The intra-day and inter-day reproducibility results are shown in Table 6. The RSD values ​​of intra-day and day-to-day test results did not exceed 5.0%, indicating good intra-day and day-to-day reproducibility.

[0059] Table 6 Intra-day and inter-day reproducibility test results

[0060]

Embodiment 3

[0061] Example 3 sample recovery experiment

[0062] Accurately weigh 1.00g of the cosmetic to be tested and place it in a 10mL stoppered colorimetric tube, add methanol to dissolve, add 500μL of standard series b (Table 2) to each sample, then dilute to the mark with methanol, ultrasonic power 2kW, Extract for 15 minutes, centrifuge at 6000 r / min for 5 minutes, and filter with a 0.45 μm filter membrane to obtain the test solution. Draw 10.0 μ L of the test solution, and measure according to the chromatographic conditions of Example 1, the average recovery range is 80.18% ~ 114.59%, and the RSD value range is 0.69% ~ 4.18% ( n =6) (see Table 7). This result shows: the method of the present invention has better recovery rate.

[0063] The sample recovery test result of table 711 kinds of standards

[0064]

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Abstract

The invention discloses a method of detecting the content of a preservative in a cosmetic, and the method comprises the following steps of (1) treating a sample to be detected; (2) preparing a preservative standard product stock solution and a standard series; (3) detecting by using a high performance liquid chromatography; (4) drawing a standard curve of a preservative standard mixed solution series, performing linear regression on the peak area of a preservative and the corresponding mass concentration, and calculating the content of the preservative in the sample to be detected according to an obtained equation of linear regression.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, further belongs to the safety field of daily chemicals, and specifically relates to a method capable of sensitively and accurately detecting the contents of various preservatives in cosmetics. Background technique [0002] The purpose of using preservatives in cosmetics is to protect products from microbial contamination, extend product shelf life, ensure product safety, and prevent consumers from possible infections caused by using microbially contaminated products. Preservatives can be broadly divided into chemical preservatives, safe non-toxic preservatives and natural organic preservatives. At present, chemical preservatives are the most commonly used in cosmetics. Usually a small amount of preservatives will not irritate the skin, but most types of preservatives are more or less toxic to a certain extent. Some manufacturers use controversial preservatives or add preservatives i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 朱丹孙文通陈彬何广新张红宇李海山侯英
Owner 云南省食品药品检验所
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