175results about How to "Low limit of quantitation" patented technology

This invention provides a method of nucleic acid analysis that enables highly accurate and sensitive quantitation by counting the number of molecules among a plurality of types of genes without amplifying specific genes and that enable reduction of quantitation limits. This method comprises steps of: allowing a polynucleotide comprising a first region having a sequence complementary to the target gene at the 3′ end, a second region having a sequence complementary to the target gene at the 5′ end, and a third region corresponding to a detection probe to hybridize to the target gene; allowing the 3′ end of the first region hybridized to the target gene to ligate to the 5′ end of the second region so as to obtain a circularized polynucleotide; with the use of the circularized polynucleotide as a template, performing a primer extension reaction using a primer having a sequence complementary to part of the circularized polynucleotide and a strand-displacement DNA polymerase; allowing a detection probe containing a sequence identical to the third region to hybridize to a sequence complementary to the third region that iteratively appears in a single-stranded portion of the extension product; and optically detecting the quantity of the detection probe hybridized to the extension product to thereby quantitate the target gene.

The invention relates to the field of analysis and testing and particularly relates to a method for analyzing residual veterinary drugs in mutton. The method at least comprises sample pretreatment, enzymatic hydrolysis, extraction, purification and on-machine testing. Compared with the existing detection method, the method provided by the invention has higher accuracy, extracts, purifies and testsa variety of veterinary drugs, and is fast and efficient. The method utilizes a multi-extraction method using three different solvents, fully extracts the veterinary drugs in mutton as many as possible, improves the detection accuracy and reduces the influence caused by the base on the veterinary drugs. In gradient elution, three mobile phases are used so that it is solved that the original two mobile phases with solubility under action of an adsorbent in a chromatographic column are layered so that the target drugs are respectively dissolved in two solvents, the characteristic peak detectedby the detector splits into a primary characteristic peak and a secondary characteristic peak, the drug content capable of being detected is reduced and the determination of other drugs is influenced.

The invention relates to a method for detection of 25-hydroxyvitamin D in serum. The method comprises sample pretreatment: adding an acetonitrile solution containing a 25-hydroxyvitamin D internal standard substance into a serum sample, carrying out protein precipitation, carrying out centrifugation, taking the supernatant and diluting the supernatant through acetonitrile to obtain a sample to be detected, wherein a volume ratio of the serum sample to the acetonitrile solution is 1: 1-4 and a volume ratio of the supernatant to acetonitrile is 1: 1-4, and enrichment, separation and detection: carrying out enrichment, separation and detection on the sample to be detected through a two-dimensional liquid chromatography-tandem quadrupole mass spectrometer. The method is simple, greatly shortens the detection time, improves the detection flux of the sample and has a high detection precision, a low cost, specificity and strong matrix interference resistance.

The invention relates to a method for detecting characteristic component of usneaceae plant in a tobacco sample. The method comprises the following steps: a) extracting the tobacco sample by an internal standard substance-containing extraction solvent to obtain a sample solution; b) using characteristic component of the usneaceae plant, an internal standard substance and the extraction solvent to prepare the standard solution with a series of concentration; c) using a gas chromatography-mass-spectrography method for determining the characteristic component of the usneaceae plant in the standard solution with a series of concentration, drafting a standard work curve; d) using the gas chromatography-mass-spectrography method to determine the content of the characteristic component of the usneaceae plant in the sample solution, employing an internal standard curve method for quantification; and e) calculating the content of the characteristic of the usneaceae plant in the tobacco sample. The method has the advantages of good repeatability and high analysis detection sensitivity.

The invention discloses a magnetic covalent organic framework compound solid phase extraction adsorbent and a preparation method and belongs to the technical field of analytical chemistry and food safety inspection. The invention provides the magnetic covalent organic framework compound solid phase extraction adsorbent and the preparation method. By coordinating Fe ions and N atoms in TpBD and taking a COF-(TpBD) / Fe3O4 mixture obtained by a coprecipitation method as an adsorbent, the adsorbent is used for extracting and purifying aromatic compounds in a food matrix, in particular a phthalate target. The method simplifies a process of pre-treatment of a sample, and the magnetic adsorbent is synthesized by a coprecipitation method. The adsorbent is simple and quick and easily available, andthe extraction workload is reduced. The detection limit of a phthalate compound is greatly reduced.

The invention discloses a method of detecting the content of a preservative in a cosmetic, and the method comprises the following steps of (1) treating a sample to be detected; (2) preparing a preservative standard product stock solution and a standard series; (3) detecting by using a high performance liquid chromatography; (4) drawing a standard curve of a preservative standard mixed solution series, performing linear regression on the peak area of a preservative and the corresponding mass concentration, and calculating the content of the preservative in the sample to be detected according to an obtained equation of linear regression.

The invention relates to a second-order mass spectrometric detection method for bisphenol substances in a water environment, and belongs to the field of bisphenol substance detection. The problems that an existing analysis method for bisphenol pollutants is low in accuracy and precision due to interference of a natural water body matrix are solved. According to the second-order mass spectrometric detection method for the bisphenol substances in the water environment, an HLB column is adopted for solid-phase extraction, an amino column is purified, a full scanning mode is selected and used for qualitative and quantitative detection, all target objects used in a sample can be detected out at the same time by means of the mode, the mode is used for analyzing trace substances in a complex matrix, and for each substance, only one or two specific pairs of parent ions and daughter ions are selected to be detected. Matrix interference can be effectively removed, and sensitivity of detection and accuracy of qualitation and quantitation are improved.

The invention discloses an analytic method for related substances in raw material and a preparation of trelagliptin succinate. A high performance liquid chromatography is adopted, and chromatographic conditions include that a chromatographic column is octadecyl silane bonded silica gel chromatographic column, the mixed solution with the pH of 3.3-3.7 and the volume ratio of phosphate buffer aqueous solution to acetonitrile of (88-92):(8-12) is taken as a mobile phase A, the mixed solution with the pH of 3.3-3.7 and the volume ratio of phosphate buffer aqueous solution to acetonitrile of (18-22):(78-82) is taken as a mobile phase B, detection wavelength is 218-222nm and isocratic elution is carried out. The analytic method disclosed by the invention can detect many impurities and can rapidly, effectively and accurately monitor the related substances in trelagliptin succinate.

The invention discloses a real-time fluorescence quantitative PCR (polymerase chain reaction) detection method for a porcine circovirus type 2, which includes the steps: 1 designing and synthesizing specific primers and probes, 2 obtaining target fragments by preparing a PCR reaction system and reacting according to PCR reaction procedures, 3 preparing ligation reaction liquid, 4 converting and extracting plasmid templates, 5 performing qualitative PCR detection, and 6 performing quantitative PCR detection. The detection method is simple, rapid, high in sensitiveness, high in specialty, fine in repeatability and extremely low in quantitative detection limit and detection limit, and the porcine circovirus type 2 can be detected quantitatively and accurately by the aid of the high sensitiveness, so that epidemic of the porcine circovirus type 2 can be prevented and controlled.

The invention provides a method of measuring multiple preservative, sweetener and colorant in food at the same time. The method comprises the following steps of 1, weighing and taking 5-10 g of a sample and placing the sample into a color comparison tube of 50 ml, adding water and dissolving the sample, implementing ultrasonic extraction for 30 minutes, if the protein content is high, needing to add a protein precipitant to regulate the pH value to be neutral, adding water to implement constant volume, mixing evenly, and flowing through a microporous membrane of 0.45 micrometer for use; 2, taking a sample solution to implement liquid chromatographic analysis, and calculating and obtaining preservative, sweetener and colorant contents in the sample to be measured against a standard curve. According to the method of measuring multiple preservative, sweetener and colorant in food at the same time, multiple preservatives, sweeteners and colorants in food can be measured at the same time, the accuracy rate and recovery rate of measured samples are guaranteed, it is ensured that the relative standard deviation is in an allowable range, the applicable range of the method is also ensured, the experiment efficiency is improved, and the laboratory cost is saved.

The invention belongs to the chemical field of medicines, and particularly relates to a method for measuring the contents of 5-soquinolinesulfonic acid methyl ester and 5-isoquinolinesulfonic acid ethyl ester in fasudil hydrochloride through an LC-MSMS (Liquid Chromatography-Tandem Mass Spectrum) method. Through HPLC (High Performance Liquid Chromatography), the problems that 5-soquinolinesulfonicacid methyl ester and 5-isoquinolinesulfonic acid ethyl ester can not be simultaneously measured on a single liquid phase condition, in addition, a moving phase greatly damages a chromatographic column and the chromatographic column is non-durable to cause poor method reproducibility can be solved. The LC-MS method is high in detection sensitivity, two ingredients are simultaneously detected, themethod is good in durability and high in accuracy, analysis time is shortened, and detection efficiency is improved.

The invention discloses a method for detecting the efficiency for intercepting phenol compounds in flue gas by a filter-tip. The method comprises the following steps: longitudinally cutting a used cigarette filter-tip along an axis of the filter-tip by a blade, placing the cut cigarette filter-tip into an extract liquor, performing ultrasonic extraction, standing the obtained extract liquor, enabling the obtained extract liquor to pass through a filtering membrane, taking the filtering liquid to perform sample introduction, analyzing by adopting an HPLC (High Performance Liquid Chromatography) to measure the content of phenol compounds; calculating the intercepting efficiency of phenol compounds in the filter-tip through measuring the phenols in main stream smoke. The method can quickly, precisely, directly and quantitatively measure the interception amount and intercepting efficiency of phenol compounds by the filter-tip, and is simple to operate, excellent in measuring precision, and low in detection limit and quantitative limit, thereby being used for the measurement of the intercepting efficiency of most of the conventional mainstream filter-tips.

The invention relates to an identification method of fake and shoddy meat floss products. The identification method is used for identifying adulteration of meat floss via detecting pigments in meat floss, and can be used for detecting artificial pigments, including sunset yellow, lemon yellow, ponceau 4r, and basic orange II in meat floss at the same time. It is determined that whether adulteration of meat floss is performed via detecting whether meat floss contains artificial pigments. According to the identification method, the artificial pigments are easily water soluble, and the solubility in most organic solvents is extremely low, so that fat components are removed firstly, salting out is adopted for coagulation sedimentation of proteins so as to avoid protein influences, solid phase extraction is adopted to avoid polysaccharide and natural pigment influences, and concentration of a sample solution is carried out. The identification method is capable of solving a problem that solid phase extraction recovery rate of basic orange II is low; separation effect is excellent; specificity is high; detection limit and quantitation limit are low enough; linearity range is wide; repeatability is high; and the identification method is accurate and reliable, and can used for daily monitoring and detection.

The invention provides a method for detecting drugs in human bodies. The method comprises the following steps of: preparing a series of mixed drug standard solutions with gradient concentrations by using 14 drug standards; respectively performing mass spectrum detection on a sample and the mixed drug standard solutions by adopting a liquid chromatography-tandem mass spectrometry (LC-MS / MS), and determining one or more than one drugs in the mixed drug standard solutions in the sample on the basis of a mass spectrum detection result so as to achieve qualitative analysis; drawing a standard curve, detecting the peak area of a parent ion / child ion pair corresponding to at least one of the 14 drugs in the sample, and obtaining the concentration of the sample according to the standard curve for quantitative analysis, wherein the sample is optionally derived from human saliva, and optionally, the sample is pretreated. The method for detecting drugs in human bodies has the advantages of convenient material obtaining, the small dosage, the low detection limit and quantitative limit, the high accuracy, the high efficiency and the like.

The invention discloses a method for detecting the content of methylene blue in blood plasma. The method comprises the following steps of: (a) adding carboxymethyl-beta-cyclodextrin saturated solution into blank blood plasma, enabling the concentration of the mixed solution to reach 1-4.5mmol / L and maintaining the constant volume to obtain a blood plasma blank sample; (b) adding quantitative methylene blue solution into the blank blood plasma and adding the carboxymethyl-beta-cyclodextrin saturated solution to obtain a standard blood plasma sample containing the methylene blue with the concentration of Cs; and (c) adding the carboxymethyl-beta-cyclodextrin saturated solution in a blood plasma sample containing the methylene blue to obtain a blood plasma to-be-measured sample containing the methylene blue with the concentration of Cs; detecting the strength or the area of a fluorescence peak of the sample; and then calculating according to a reference substance comparison method to obtain the concentration Cs of the methylene blue in the blood plasma to-be-measured sample. According to the method disclosed by the invention, the lowest detectable limit is 0.012mumol / L and the unexpected effect is obtained.

The invention relates to a liquid chromatographic analysis method for detecting acrylamide in fried food. The liquid chromatographic analysis method for detecting acrylamide in fried food comprises the following steps: taking ground and pulverized fried food, adding an acrylamide standard substance solution, adding n-hexane, carrying out eddy vibration, removing a petroleum ether layer, adding deionized water, carrying out eddy vibration and constant-temperature oscillation, carrying out ultrasonic oscillation, adding Carrez reagents Carrez I and Carrez II to remove protein, centrifuging at high speed, enabling supernate to pass through activated SPE small columns, and filtering to obtain a test article solution; and analyzing the test article solution by using a liquid chromatography. The acrylamide is heated and extracted with deionized water and is ultrasonically extracted, the extracting rate is 98.2%, and RSD is equal to 2.83%; the deionized water is safe and non-toxic, and is low in price; and the detection limit and the limit of quantitation are low, the detection limit is 0.002 mu g.mL-1, and the limit of quantitation is 0.005 mu g.mL-1. The liquid chromatographic analysis method is rapid, accurate, efficient and safe, and can accurately detect the content of the acrylamide in the fried food.

The invention provides a high performance liquid chromatography-ultraviolet detection method for simultaneously detecting specific migration amounts of nine anti-oxidants in five food simulants. The method comprises immersing a sample to be measured in food simulants, carrying out cooling to a room temperature, carrying out uniform mixture, metering a volume of the water-based food simulants by a tris(2-carboxyethyl)phosphine hydrochloride-containing tetrahydrofuran mixed solution, carrying out filtration by a hydrophilic Teflon syringe filter, carrying out sample introduction, carrying out rotary distillation nitrogen-blowing condensation on the isooctane food simulants until basic drying, metering the volume by tris(2-carboxyethyl)phosphine hydrochloride-containing methanol, carrying out filtration by the hydrophilic Teflon syringe filter and carrying out sample introduction. C8 column gradient elution separation is used, detection wavelength is 285nm and a sample size is 20 microliters. The method has good chromatographic separation and linear relationship, has high recovery rate and high accuracy, has a quantification limit satisfying limitation requirements on the total migration amount of the nine anti-oxidants in the EU NO 10 / 2011 regulation and has been used for detection of a practical sample.

The invention discloses a method for measuring a volatile organic compound in a tobacco material. The method comprises the steps of using a headspace sampler and ion molecular reaction mass spectrometry (IMR-MS) in a combination way, collecting a volatile constituent in tobacco material paper (including cigarette paper, tipping paper, hard box package paper, soft box package paper, strip package paper, tobacco filter stick and filaments by a headspace sampler, analyzing by the IMR-MS, and achieving rapid and accurate measurement of the content of the volatile organic compound in the tobacco material by drawing a standard curve and by calculation. By the method, relatively-simple clean mass spectrum can be obtained, the qualitative and quantitative analysis of the volatile organic compoundis simplified, 19 types of volatile organic compound common in the tobacco material can be simultaneously, accurately and quantitatively detected, the advantage of rapid analysis is beneficial for achieving detection of a large amount of volatile samples, and the rapid analysis demand of a laboratory is satisfied.

The invention discloses a method for utilizing matrix solid-phase dispersion extraction to analyze plant organophosphorus ester and relates to a plant organophosphorus ester analyzing method. The method comprises the steps: cutting up a plant sample, grinding the plant sample with an adsorbent, transferring a mixture into an empty solid-phase extracting column, eluting the solid-phased extractioncolumn, collecting and concentrating eluant, utilizing a solvent to dissolve the eluant, then utilizing a gas chromatography-serial triple quadrupole mass spectrum to perform qualitative and quantitative analysis on 13 kinds of organophosphorus ester. In the method disclosed by the invention, the sample is extracted and purified at the same time, an operation process is simple, an organic solventuse amount is small, a sample use amount is smaller, and consumed time is short; by means of the gas chromatography-serial triple quadrupole mass spectrum, selectivity and flexibility of target compound are improved; an adding standard recovery rate of the 13 kinds of organophosphorus ester is 65.1% to 109.1%, relative standard deviation is smaller than 15%, and good accuracy and precision are achieved; a detection limit of the method is 0.05ng / g to 0.33ng / g, a quantitation limit of the method is 0.16ng / g to 1.10ng / g, and the method can be applied to trace analysis.

The invention provides a method for detecting the content of hydroxylamine hydrochloride in azilsartan. According to the method, hydroxylamine is subjected to derivatization treatment by adopting benzaldehyde, octadecylsilane chemically bonded silica is used as a filler of a chromatographic column, a mobile phase A is a 10mmol / L ammonium formate solution (containing 0.1% of formic acid), a mobilephase B is acetonitrile, gradient elution is adopted, a sample does not need to be specially treated, and matrix influence is avoided. According to the detection method, azilsartan is adopted as a test sample, and the system applicability, specificity, detection limit, durability and other items prove that azilsartan meets related requirements.

The invention discloses an analytical method of gonyautoxins detected by combination of molecular imprinting solid phase extraction-hygroplasm, and belongs to the field of front treatment and chemical analysis and detection of a biological sample. The method applies a molecular imprinting polymer to structure a three-dimensional cavity and a specific recognition site which are highly similar as the imprinting molecule, so as to realize the selective recognition of the imprinting molecule, and improve efficiency of gathering target objects and removing interferent; moreover, an analytical method of hygroplasm combination detected target taking cyan column as a chromatograph column is developed; the cyan column has strong polarity in use of reverse phase, thus polarity target objects and impurities in the sample can be effectively separated; then better separating effect and mass spectrometric detection effect of gonyautoxins on the chromatographic column can be obtained according to the optimized hygroplasm condition. The method is featured by small sample demand, high recycle rate and precision, and low detection limit; the matrix effects in detection are reduced; the analytical method is applicable to the extraction and detection of multiple shellfish samples, and has very good universality.

The invention relates to the technical field of veterinary drug residue detection, in particular to a method for analyzing residual antibiotics in beef. The method at least comprises the steps that beef samples are subjected to pretreatment, extraction, purification and monitoring analysis, wherein the monitoring analysis is conducted by means of a chromatogram-mass spectrometry combined technology. In the extraction process, a surfactant is adopted for increasing a dissolution rate of the target antibiotics; in the purification process, a demulsifier is adopted for reducing an emulsificationrate of a system, matrices and other impurities are precipitated and removed, and the precision and sensitivity of the detection process are further improved.

The invention relates to a method of using a liquid chromatography tandem mass spectrometry for synchronously measuring four kinds of antibiotics in river sediment. The method of using the liquid chromatography tandem mass spectrometry for synchronously measuring the four kinds of antibiotics in the river sediment includes the following steps that 1, an experimental vessel is prepared; 2, the moisture content is measured; 3, vacuum freeze drying is conducted; 4, sequential extraction, grinding and sieving are conducted; 5, ultrasonic extraction and mechanical vibration are used for preparation; 6, double-column tandem solid-phase extraction is carried out, wherein before the solid-phase extraction, a buffer solution is added into an extraction solution subjected to rotary evaporation, then, ultrapure water is adopted to dilute a mixture, and the mixture is shaken to be fully uniform; 7, the extraction solution is purified and subjected to elution; 8, concentration and constant volume are conducted; 9, high-performance liquid chromatography is adopted to carry out synchronous computer measurement. By the adoption of the method of using the the liquid chromatography tandem mass spectrometry for synchronously measuring the four kinds of antibiotics in the river sediment, the purification and enrichment effects are greatly improved, 0.2% methanoic acid-2mmol / L ammonium acetate is used, acetonitrile and a citric acid buffer solution of which the pH value is 3 are used as the extraction solution of a solid sample, and optimal conditions are adopted to be configured and combined,so that a target analyte is better isolated, chromatographic peak area is larger, and the recovery rate is higher.

The invention provides a method for simultaneously separating and quantifying multiple components. The method comprises the steps of sample pretreatment and sample detection. The method provided by the invention has good specificity, and can avoid the interference of various components, especially components (such as polyethylene glycol and povidone) which are easy to interfere with each other, inmeasurement. The invention provides a good method for reverse engineering, component analysis, release detection of auxiliary materials and the like of pharmaceutical preparations and other products(such as cosmetics and foods). The method is simple to operate, good in specificity and accuracy, wide in linear range and high in sensitivity, and the detection capability and the detection efficiency are greatly improved.

The invention discloses a fluorescent quantitative PCR (polymerase chain reaction) detection kit for hepatitis B virus nucleic acid. The quantitative detection kit for the hepatitis B virus nucleic acid comprises primers and probes aiming at an S gene and / or a C gene, wherein the sequences of the primers are as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4 in the specification, the sequences of the probes are as shown in SEQ ID NO.5 to SEQ ID NO.7 in the specification, and the three probes are respectively marked with fluorescence. The high-sensitivity quantitative detection kit for the hepatitis B virus nucleic acid has the advantages that HBV genome DNA molecules are detected, the quantitative range is wide, the sensitivity can reach 0.5 IU / ml of HBV DNA, the quantitative limit is as low as 2 IU / ml of HBV DNA, and the detection limit is as low as 1 IU / ml of HBV DNA; and in addition, a method and the kit are adopted, by means of the multi-functional utilization of theprimers and the probes at the nucleic acid invasion reaction stage, the complexity of the probe design and a detection system can be further reduced, and the economic cost of the probe design and thedetection system can be lowered.

The invention provides a high performance liquid chromatography detection method for anthocyanin in blueberries, and belongs to the technical field of photoelectrode materials. The anthocyanin comprises delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, petunidin-3-O-glucoside, pelargonidin-3-O-glucoside, paeoniflorin 3-O-glucoside and malvidin 3-O-glucoside, wherein the flow rate of the high performance liquid chromatography is 0.8 mL / min, the detection wavelength is 520 nm, the detection temperature is 25 DEG C, the sample injection volume is 10 microliters, the detection time is 60 minutes, mobile phases: an aqueous phase B is a 0.3 wt% phosphoric acid aqueous solution, and an organic phase A is acetonitrile, and gradient elution. According to the present invention, the simultaneous determination of the six anthocyanins in the blueberry sample is achieved, the standard deviation of the peak area and the retention time is small, the detection limit and the quantification limit of the six anthocyanins are low, and the method has characteristics of high sensitivity, good precision, good reproducibility and good stability.

The invention relates to a high performance liquid chromatography-tandem mass spectrometry detection method for vitamin K1 in serum. The method comprises the following steps: sample pretreatment: adding a precipitant into a serum sample to be detected for precipitation; adding an extracting agent for extraction and centrifugation; drying the obtained supernate, redissolving with a redissolving solution, and centrifuging; taking the supernate as a to-be-detected sample solution; the precipitant being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol, an internal standard substance and ethanol; the extraction agent being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol and normal hexane; the reconstitution fluid being prepared from 2, 6-di-tert-butyl-4-methylphenol, estriol and methanol; performing liquid chromatography separation: performing chromatographic separation on the to-be-detected sample solution by using high performance liquid chromatography; and performing mass spectrum detection: carrying out mass spectrum detection on the sample separated by the high performance liquid chromatography. The method provided by the invention has the advantages of simple steps, strong specificity, high sensitivity and good anti-interference capability.

The invention discloses a multi-component content determination method of Miao medicine Laportea bulbifera. The method comprises the following steps: S100, preparation of mixed reference solution, respectively and accurately weighing right amount of reference substances of Gallocatechin, neochlorogenic acid, Epigallocatechin, catechinic acid, chlorogenic acid, Cyclohexanecarboxylic acid, epicatechin, rutin, isoquercitrin, kaempferol-3-0-rutinoside and quercitrin; and simultaneously determining contents of eleven components including the Gallocatechin, the neochlorogenic acid, the Epigallocatechin, the catechinic acid, the chlorogenic acid, the Cyclohexanecarboxylic acid, the epicatechin, the rutin, the isoquercitrin, the kaempferol-3-0-rutinoside and the quercitrin. The method is simple, efficient, highly sensitive and excellent in specificity, and can provide baisis for quality control of Laportea bulbifera medicinal materials. Compared with the ordinary liquid phase, the method has the advantages of being accurate, reliable and highly sensitive, and having lower specificity detection limit and quantification limit, and is more effective in medicinal material component content analysis through a liquid chromatography-mass spectrometry analysis technology.

The invention belongs to the technical field of pharmaceutical component analysis, and particularly relates to a method for detecting the content of compounds in Huoxiang Zhengqi oral liquid through liquid chromatography-mass spectrometry. The method comprises the following steps: carrying out gradient elution separation by using liquid chromatography, and then carrying out mass spectrometric detection, in the detection process, liquiritin, glycyrrhizic acid, hesperidin, naringin, magnolol, honokiol and pachymic acid A are detected in a negative ion scanning mode; detection in a positive ion scanning mode shows that the corresponding NCE energy of liquiritin is 50 V, the corresponding NCE energy of glycyrrhizic acid is 30 V, the corresponding NCE energy of hesperidin is 50 V, the corresponding NCE energy of naringin is 30 V, the corresponding NCE energy of magnolol is 70 V, the corresponding NCE energy of honokiol is 70 V, the corresponding NCE energy of pachymic acid A is 50 V, the corresponding NCE energy of water and oxidized peucedanin is 50 V, and the corresponding NCE energy of nobiletin is 50 V. The detection method is good in repeatability and precision, and can be used for qualitatively and quantitatively detecting hydrated oxypeucedanin and nobiletin with relatively low contents in the hydrated oxypeucedanin and nobiletin.

The invention discloses an analysis method for detecting impurities in aspartate ornithine and belongs to the field of analytical chemistry. The analysis method comprises the following steps: by usingliquid chromatography, under the conditions that an amino bonded silica gel filling agent, namely Thermo APS-2 of 4.6 mm*250 mm*5 [mu]m, is adopted, 0.1 mol / L monopotassium phosphate buffer solution-acetonitrile (35:65) is used as a mobile phase A, 0.1 mol / L monopotassium phosphate buffer solution-acetonitrile (50: 50) is used as a mobile phase B, a detection wavelength is 200 nm, a flowing speedis 1.0 ml / minute, and a column temperature is 30 DEG C, performing gradient elution, and performing analysis. By adopting the technical scheme, impurities such as 3-amino-2-piperidone, di-poly-l-arginine and an alpha-aspartate biopolymer and an aspartate condensation compound can be rapidly and accurately qualitatively and quantitatively analyzed, a good separation degree can be achieved, a relatively low detection limit and quantitative limit can be achieved, a relatively wide linear range can be achieved, the contents of different impurities can be relatively accurately calculated, and technical support can be provided for further monitoring and studying impurities in the aspartate ornithine.