Method of measuring multiple preservative, sweetener and colorant in food at the same time

A technology for preservatives and sweeteners, applied in the field of food chemical composition detection, can solve cumbersome problems and achieve the effects of improving experimental efficiency, ensuring accuracy and recovery rate, and saving costs

Inactive Publication Date: 2017-08-11
安徽省公众检验研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] For various preservatives, sweeteners and colorants in food, the current national standards measure several substances separately, which is cumbersome

Method used

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Embodiment Construction

[0015] In order to better understand the present invention, the following will be further described in conjunction with specific examples.

[0016] A method for simultaneously determining multiple preservatives, sweeteners and colorants in food, comprising the following steps:

[0017] 1) Weigh 5-10g of the sample into a 50ml colorimetric tube, add water to dissolve it, and extract it by ultrasonic wave for 30 minutes. If the protein content is high, add a protein precipitant to adjust the pH to neutral, add water to volume, and mix well. Pass through a 0.45 μm microporous membrane, set aside;

[0018] 2) Take the filtrate and carry out liquid chromatography analysis, and calculate the content of preservatives, sweeteners and colorants in the sample to be tested by comparing with the standard curve.

[0019] The preservative is one or more of sorbic acid, benzoic acid, dehydroacetic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, butyl methy...

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Abstract

The invention provides a method of measuring multiple preservative, sweetener and colorant in food at the same time. The method comprises the following steps of 1, weighing and taking 5-10 g of a sample and placing the sample into a color comparison tube of 50 ml, adding water and dissolving the sample, implementing ultrasonic extraction for 30 minutes, if the protein content is high, needing to add a protein precipitant to regulate the pH value to be neutral, adding water to implement constant volume, mixing evenly, and flowing through a microporous membrane of 0.45 micrometer for use; 2, taking a sample solution to implement liquid chromatographic analysis, and calculating and obtaining preservative, sweetener and colorant contents in the sample to be measured against a standard curve. According to the method of measuring multiple preservative, sweetener and colorant in food at the same time, multiple preservatives, sweeteners and colorants in food can be measured at the same time, the accuracy rate and recovery rate of measured samples are guaranteed, it is ensured that the relative standard deviation is in an allowable range, the applicable range of the method is also ensured, the experiment efficiency is improved, and the laboratory cost is saved.

Description

technical field [0001] The invention relates to the technical field of food chemical component detection. In particular, it relates to a method for simultaneously determining multiple preservatives, sweeteners and colorants in food. Background technique [0002] For various preservatives, sweeteners and colorants in food, the current national standards measure several substances separately, which is relatively cumbersome. Benzoic acid, sorbic acid and sodium saccharin use GB / T 23495-2009 "Determination of Benzoic Acid, Sorbic Acid and Sodium Saccharin in Food by High Performance Liquid Chromatography" and GB / T 5009.29-2003 "Determination of Sorbic Acid and Benzoic Acid in Food". Determination" GB / T5009.28-2003 "Determination of Sodium Saccharin in Food", acesulfame K is used in GB / T 5009.140-2003 "Determination of Acesulfame Potassium in Beverages", dehydroacetic acid GB / T 23377-2009 "Food Determination of dehydroacetic acid in medium-high performance liquid chromatography...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 朱磊磊李维王天雨
Owner 安徽省公众检验研究院有限公司
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