34 results about "Ergostanol" patented technology

The scheme provides a kind of engineered exosome double drug delivery system loaded with ergosterol and its preparation method and application, belongs to the field of nanomaterials and nanobiomedicine, based on the innovative ROS-responsive nanomaterial CHPG design, load the active ingredient ergosterol to play the role of treatment and repair, which realizes the blood-brain barrier penetration and precise drug release in lesion area through targeted functional modification;It is wrapped in the nasal mucosa-derived stem cell exosome to form a drug delivery system Exo@Erg-CHPG-M, which combines the natural biological characteristics of exosome and the precise targeting advantage of nanomicelle, significantly improves the drug delivery efficiency, in vitro and animal model verification show that, the system can improve the neurological impairment and brain tissue injury after ischemic stroke by synergistically regulating inflammation and neural repair pathways, the scheme provides a new technical scheme for the precise treatment of central nervous system diseases such as cerebral stroke.

The invention discloses a method for increasing the content of ergosterol in tremella aurantialba mycelia by using adenosine, and relates to the technical field of biological ferment.The method comprises the following steps that a sterilized liquid culture medium is inoculated with tremella aurantialba strains, and liquid culture is conducted to obtain a liquid culture solution; centrifuging the liquid culture solution to obtain mycelia; the content of ergosterol in the obtained mycelium is greater than 34.6 mg / L; a liquid fermentation culture medium is composed of 200 g / L of potatoes, 20 g / L of glucose, 3 g / L of monopotassium phosphate, 1.5 g / L of magnesium sulfate and 12.0 mg / L of vitamin B1, water is added to make the volume constant to 1000 mL, and the pH value is natural; adding adenosine into the liquid culture medium until the concentration of adenosine is greater than 1.0 g / L; the classification of the inoculated tremella auramtialba strain is named as Naematia auramtialba, and the classification is named as Naematia auramtialba; the preservation number of the strain is CGMCC (China General Microbiological Culture Collection According to the method, the content of ergosterol in the obtained tremella aurantialba mycelia can be increased to 45.8 mg / L and the biomass to 12.5 g / L under the condition that the biomass of the mycelia of the tremella aurantialba No. 2 ZJJE002 (the preservation number is CGMCC NO.40124) in the tremella aurantialba strain is not influenced, and the content of ergosterol is 1.55 times that of a control group.

The invention provides an ergosterol-loaded engineered exosome double-drug-loading system as well as a preparation method and application thereof, belongs to the field of nano materials and nano biological medicines, and is based on innovative ROS response type nano material CHPG design, a therapeutic active component ergosterol is loaded to play a role in treatment and repair, and the curative effect of the ergosterol is improved. Blood-brain barrier penetration and accurate drug release in a focus area are realized through targeted function modification; the Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG-M / Erg-CHPG- the nerve function defect and brain tissue damage after ischemic stroke are improved, and a new technical scheme is provided for precise treatment of central nervous system diseases such as cerebral stroke.

The invention discloses a bactericidal composition containing meconazole and captan, and relates to the technical field of pesticide preparation, the bactericidal composition is prepared by mixing a meconazole microcapsule suspending agent and a captan suspending agent, and the weight ratio of the meconazole microcapsule suspending agent to the captan suspending agent is 1: 20-20: 1. A double-target synergistic effect is formed through compounding of the meconazole and the captan, the meconazole serves as a C14-demethylation inhibitor containing an isopropanol group, the cell membrane function of thalli is destroyed by preventing ergosterol biosynthesis, the unique molecular structure of the meconazole can freely rotate to be combined with a target, pathogenic bacteria mutation is reduced, resistance development is delayed, and the anti-bacterial effect is achieved. And a new tool for efficiently preventing and treating diseases caused by resistant strains is provided for planters.

The invention discloses a method for detecting the content of various components in a puffball sample and application of the method, and the method comprises the following steps: taking puffball sample powder, adding an extraction solvent for extraction, and preparing to obtain a test solution; taking alpha-linolenic acid, linoleic acid, palmitic acid, oleic acid, stearic acid and ergosterol reference substances, and adding a solvent to prepare a mixed reference substance solution; respectively injecting the mixed reference substance solution and the test solution into an ultra-high performance liquid chromatograph for detection, and comparing the maps of the reference substance solution and the test solution; and making a regression equation by using the sample size Ln value and the peak area Ln value of each component, and calculating the content of the components in the puffball sample. According to the quality detection method for the puffball sample, the contents of the six components can be simultaneously determined, the operation is simple, the accuracy is high, the recovery rate is reliable, the detection efficiency is high, and a basis is provided for standardizing the market of puffball medicinal materials and preparations and reasonably developing the puffball medicinal materials and preparations.

The invention provides application of ergosterol in preparation of a medicine for treating pulmonary arterial hypertension. The preparation method of the ergosterol comprises the following steps: cleaning, crushing and drying silkworm chrysalis cordyceps hainanensis sporocarp to obtain cordyceps militaris powder; the method comprises the following steps: mixing cholic acid, deoxycholic acid, methoxypolyethylene glycol and absolute ethyl alcohol to obtain ethanol mother liquor of a supramolecular solvent; carrying out gradient synergistic extraction on the cordyceps militaris extract; carrying out reduced pressure distillation and drying to obtain an extract, and carrying out chromatographic separation and drying; and recrystallizing, cleaning and drying to obtain the product. According to the invention, CO2 expanded ethanol liquid is adopted as a solvent, a cholic acid-deoxycholic acid-methoxypolyethylene glycol supramolecular solvent system technology and homologous adaptability of steroid nucleus structures of etocholic acid and deoxycholic acid and ergosterol are utilized to construct a multi-stage cavity recognition network, and C5-C6 double bonds and C22-C23 double bonds of ergosterol are specifically combined, so that the detection sensitivity of ergosterol is improved, and the detection sensitivity of ergosterol is improved. Meanwhile, the pore diameter of the cavity is regulated and controlled through methoxypolyethylene glycol, and the content of ergosterol in the extract is increased.

The invention provides a 5alpha, 8alpha-peroxy ergosterol-quaternary ammonium salt derivative as well as a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. The preparation method comprises the following steps: firstly, synthesizing a peroxy-ergosterol bromate derivative by taking 5alpha, 8alpha-peroxy-ergosterol as a raw material; then, based on the molecular hybridization theory, a series of side chains with different amino substituent groups are introduced to the peroxy ergosterol bromate derivative, and a quaternary ammonium salt structure is constructed. A series of 5 alpha, 8 alpha-peroxy ergosterol-quaternary ammonium salt derivatives with clinical anti-tumor application potential are designed and synthesized. An anti-tumor activity test shows that the obtained derivative has different degrees of inhibiting effects on tumor cells of human lung cancer, liver cancer, breast cancer and the like and shows a remarkable inhibiting effect on human breast cancer MDA-MB-231 cells, and the anti-tumor activity of the derivative with the most excellent activity is improved by 69 times compared with that of a lead compound 5alpha, 8alpha-peroxy ergosterol.

The application provides application of ergosterol in preparation of a medicine for treating pulmonary arterial hypertension. A preparation method of the ergosterol is as follows: Cordyceps militaris Haining strain fruiting bodies are taken, washed, crushed, and dried to obtain Cordyceps militaris powder; cholic acid, deoxycholic acid, polyethylene glycol monomethyl ether and anhydrous ethanol are mixed to obtain an ethanol mother liquor of a supramolecular solvent; Cordyceps militaris extraction liquid is gradiently and cooperatively extracted; vacuum distillation, drying, chromatographic separation, drying, recrystallization, washing and drying are sequentially performed, and the ergosterol is obtained. In the application, CO2 expanded ethanol liquid is used as a solvent, and a cholic acid-deoxycholic acid-polyethylene glycol monomethyl ether supramolecular solvent system technology is used. Relying on the homologous adaptability of the steroid nucleus structure of cholic acid and deoxycholic acid and ergosterol, a multi-level cavity recognition network is constructed, the C5-C6 double bond and the C22-C23 double bond sites of ergosterol are specifically combined, and the content of ergosterol in the extract is improved by regulating the cavity aperture through the polyethylene glycol monomethyl ether.

The application provides a brain-targeting nano preparation loaded with ergosterol and a preparation method and application thereof, relates to the fields of nanometer materials and nanometer biological medicine, and the active ingredient ergosterol of the nano preparation is encapsulated in DSPE-PEG-pinacol boronate nanometer material, the nanometer material has ROS response characteristics, the nano preparation loaded with ergosterol is regular spherical, has uniform particle size, has good dispersibility and stability, has good ability of targeted treatment of ischemic stroke diseases, effectively solves the problems of poor targeting of ordinary drugs, low bioavailability and the like, enhances the treatment effect of ergosterol on ischemic stroke, can effectively improve brain damage caused by ischemic stroke, has good treatment effect, meanwhile, the active ingredient ergosterol plays a role in treating ischemic stroke by activating the PI3K / AKT / mTOR pathway, and provides a new direction for the treatment of ischemic stroke.

The present invention provides a yeast cell wall and a use thereof in the field of alcohol fermentation. The yeast cell wall is a yeast cell wall of Saccharomyces cerevisiae ER18 with the accession number of CCTCC NO: M 2020118. The yeast cell wall provided by the present invention is used as an encapsulation carrier of an alcohol fermentation promoter, and the encapsulation efficiency is improved by more than 30%. Moreover, the yeast cell wall is rich in ergosterol and other sterols and a plurality of unsaturated fatty acids, has a high nutritional value, and can provide a nutritional raw material for alcohol fermentation, thereby improving the yeast fermentation performance and improving the alcohol yield of alcohol fermentation.

The invention relates to the technical field of medicine synthesis, in particular to ergosterol amino alcohol derivatives with antibacterial activity and a preparation method and application thereof. The structure is as shown in general formula (I). The ergosterol amino alcohol derivative disclosed by the invention is high in gram-positive bacterium and gram-negative bacterium resisting activity, and the antibacterial activity of the derivative with the optimal activity on staphylococcus aureus and escherichia coli is improved by 64 times and 32 times respectively, so that the ergosterol amino alcohol derivative is obviously superior to that of lead compound ergosterol and compounds reported in literatures. The IC50 values of the human body normal cells HK-2 (near-end renal tubular epithelial cells) and HEK-293 (human embryo renal epithelial cells) are obviously higher, and when MIC < lt > is less than MIC < lt >, MIC < lt > is less than MIC < lt >; the ergosterol amine alcohol derivative has the selectivity index SI of more than or equal to 6.0 when the molecular weight of the ergosterol amine alcohol derivative is 32 [mu] g mL <-1 >, has the characteristics of high efficiency and low toxicity, and has potential application value in the aspect of anti-infection drug development.

The application of ceramide in the control of rice blast fungus falls within the field of biological control. This invention relates to the application of ceramide in the control of rice blast fungus: a combination of ceramide and lipopeptides is used for the control of rice blast fungus. Exogenous addition of ceramide significantly enhances the stress effect of lipopeptides on rice blast fungus. It also enhances the abnormal accumulation of glycogen and lipid droplets in conidia, promotes the bursting of ROS, and increases intracellular ceramide and calcium... 2+ The invention increases the content of malondialdehyde (MDA) in mycelium, enhances membrane lipid peroxidation, and decreases ergosterol content. Furthermore, it inhibits the expression of ergosterol synthesis genes, UPR target genes, and sphingolipid metabolism-related genes. This invention develops a highly efficient natural agent against rice blast by combining exogenous Cer with lipopeptides. This agent effectively reduces the pathogenicity of rice blast fungus and significantly inhibits its infection of rice, laying the foundation for the development of novel biological control agents.

The invention discloses a derivative derived from a natural component ergosterol in spartina alterniflora and a preparation method and application thereof. The derivative derived from the natural component ergosterol in the spartina alterniflora has the following molecular structure expression, according to the invention, a chemical derivative with a novel structure is synthesized by taking bio-based ergosterol as a raw material, and the chemical derivative is proved to have a remarkable effect on reducing the blood sugar level in vivo and is expected to be used as a potential medicine for diabetes;

The invention discloses an ergosterol derivative derived from spartina alterniflora and a preparation method and application of the ergosterol derivative. The ergosterol derivative has the following molecular structure expression formula, researches prove that the ergosterol derivative provided by the invention not only can reduce fasting blood glucose, but also has a good control effect on postprandial blood glucose, so that the ergosterol derivative shows an excellent overall blood glucose control effect and has a good clinical application prospect;

The invention provides an ergosterol nano-dispersion and a preparation method and application thereof, the ergosterol nano-dispersion comprises an ergosterol core material and a soybean protein isolate wall material wrapping at least part of the surface of the ergosterol core material, the particle size of the dispersion is 250-400 nm, and the PDI of the dispersion is 0.034-0.106. Due to the special composition and related parameters of the dispersion, the dispersity, uniformity and bioaccessibility of the ergosterol in water are remarkably improved.

The invention provides a preparation method and application of a ganoderma lucidum spore oil composition, and relates to the technical field of health food processing. The wall-broken ganoderma lucidum spore powder and plukenetia volubilis fruit seed powder are mixed and extracted, and the mass ratio of the wall-broken ganoderma lucidum spore powder to the plukenetia volubilis fruit seed powder is controlled to be (1-3): (2-4), so that the contents of ganoderma triterpenes, ergosterol, oleic acid, natural vitamin E, linoleic acid and linolenic acid in the product can be obviously increased; the ganoderma lucidum spore oil composition has obvious effects on regulating blood fat, preventing cardiovascular diseases, maintaining skin, resisting aging, protecting liver and the like, can prevent spores from oleic acid failure, can retain beneficial components in the ganoderma lucidum spore oil to the greatest extent, and obviously improves the stability of the composition.

The invention relates to the field of yeast extraction, in particular to a method for co-producing ergosterol and yeast sterol from yeast. The invention provides a method for co-producing ergosterol and yeast sterol from yeast, 4-phenyl-3H-1, 2, 4-triazoline-3, 5-diketone can selectively react with ergosterol in a sterol mixed solution, and the yeast sterol does not participate in the reaction, so that the purposes of removing the ergosterol and purifying the yeast sterol are achieved. The co-production of the yeast sterol is realized from the yeast for producing the ergosterol, and the economic value is improved while the tailing discharge is reduced. Compared with an existing technology for extracting yeast sterol from yeast, the technology does not need multi-step chromatographic separation, the process is greatly simplified, the selectivity is high, and the purity of yeast sterol is high.

The invention relates to the technical field of anti-inflammatory intestinal health oral liquid, and discloses an anti-inflammatory intestinal health oral liquid formula based on a yellow mushroom extract. Comprising the following raw materials: 3g of yellow mushroom extract (containing ergosterol), 1g of fructo-oligosaccharide, 2g of erythritol, 5g of cane sugar, 2g of xylitol, 0.2 g of pectin, 0.1 g of xanthan gum, 0.2 g of citric acid, 0.1 g of malic acid, 5ml of glycerol, 0.2 g of natural orange essence, 0.05 g of nisin, 0.05 g of vitamin C and 100ml of purified water. According to the anti-inflammatory intestinal health-care oral liquid formula based on the yellow mushroom extract, the oral liquid is orally taken for 9 days in an equivalent dose continuously in DSS induced UC mice, the colon length can be remarkably recovered, the spleen index is reduced, tissue inflammation lesion is relieved, and the anti-inflammatory intestinal health-care oral liquid formula has anti-inflammatory and health-care functions aiming at UC patients and people with sub-healthy intestinal functions, and has a good application prospect. And compared with the prior art, the active ingredients are clearer, and the stability and the taste are better.

Disclosed herein is an improved and efficient process for synthesis of vitamin D3 and its analogue Calcifediol from Ergosterol. Particularly, the present invention discloses the synthesis of key intermediate 3β-tert-Butyldimethylsilyloxy-22-hydroxy-23,24-bisnorchola-5,7-diene (5), and novel intermediate β-tert-Butyldimethylsilyloxy-22-iodo-23,24-bisnorchola-5,7-diene (9) by a simple and cost effective process. The industrially viable processes for preparation of said intermediate(s) results in providing provitamins with various side chains and the desired products in high yield.

This invention relates to the field of pharmaceutical testing technology and provides a method for sterility testing of amphotericin B liposomes for injection, comprising the following steps: mixing tryptic soy peptone liquid culture medium with Tween, ergosterol, and ethanol to obtain a culture medium containing a neutralizing agent; mixing 0.1% peptone buffer with Tween, ergosterol, and ethanol to obtain a buffer solution containing a neutralizing agent; reconstituteing the amphotericin B liposomes for injection with sterile water to obtain a liposome reconstituted solution, and diluting and mixing it evenly; filtering the diluted amphotericin B liposomes for injection using a membrane filtration method, rinsing repeatedly with the buffer solution containing the neutralizing agent, and then adding the culture medium containing the neutralizing agent; adding a standard test strain for sterility testing to the culture medium and culturing. This invention uses ergosterol as a neutralizing agent, Tween as a dispersant, and ethanol as a solvent for ergosterol, ensuring the effectiveness of the sterility testing method for amphotericin B liposomes for injection.

The present application relates to the technical field of functional food preparation and development of resources of medicinal and edible origin, and specifically relates to a compound lipid-lowering functional tablet candy of coix seed and red koji and a preparation method thereof. The present application realizes the synergistic promotion of active ingredient enrichment, stable processing characteristics and efficient lipid-lowering effect through precise control of the solid-state fermentation process of coix seed by Monascus purpureus and formula optimization based on the AHP-CRITIC mixed weighting method. The content of lovastatin in the tablet candy of the present application is 1.35 times that of the same type of commercially available red koji product, the content of phytosterols (especially ergosterol) is more than 4 times that of commercially available products, and the product retains the coixol component that is not detected in commercially available products. The raw materials of the present application are all resources of medicinal and edible origin or food-grade auxiliary materials, and have high long-term safety. The tablet candy has high standardization, is easy to take and store stably, and meets the convenience needs of consumers for daily health management.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing ergosterol and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the method, sterol synthesis route genes are screened, the speed limiting step is determined, high-yield ergosterol is preliminarily achieved through combined expression, the flow direction of carbon metabolic flow is further pulled through lipid droplet engineering and cell area chamber engineering, and the yield of ergosterol is increased. On the basis of enzyme modification engineering, the catalytic efficiency of the ERG11 on the lanosterol is improved through substrate channel engineering and proton-dependent catalytic path construction. Through multi-copy integration of the genes in the speed limiting step, the yield of ergosterol in a shake flask of the constructed engineering strain reaches 433.1 mg / L, and the yield of ergosterol in a 5L fermentation tank system reaches 4.58 g / L.

The invention belongs to the technical field of ganoderma lucidum spore oil extraction, and relates to ergosterol-enriched ganoderma lucidum spore oil and a preparation method thereof. According to the method, supercritical CO2 is adopted for extracting ganoderma lucidum spore oil, a dielectrophoresis technology is adopted for removing free fatty acid, the acid value and the peroxide value of the spore oil are reduced, rancidity of the spore oil in the storage process is prevented, a large number of fat-soluble impurities in ergosterol crystals are removed and separated out through recrystallization, and then the ergosterol crystals are enriched into the spore oil through secondary dissolution of supercritical CO2. And finally, removing the plasticizer in the spore oil by adopting a molecularly imprinted polymer, and carrying out reduced pressure distillation to prepare the ganoderma lucidum spore oil with the ergosterol content of 210 mg / 100g or above and the total triterpenoids content of 40 g / 100g or above.

The invention provides a salicylic acid nano-liposome cold damage resistant preservative and application thereof in prevention and control of fruit and vegetable cold damage, a preparation method of the salicylic acid nano-liposome cold damage resistant preservative comprises the following steps: dissolving salicylic acid, phosphatidylcholine and ergosterol in ethanol to form a uniform mixed solution; carrying out rotary evaporation on the obtained mixed solution to remove the organic solvent to form a uniform lipid film; adding a preheated phosphate buffer solution containing Tween 80 into the obtained lipid film, and hydrating for 10-20 minutes; and carrying out ultrasonic treatment and the like on the obtained product to prepare the salicylic acid nano-liposome with uniform particle size. The salicylic acid nano-liposome disclosed by the invention has a good fresh-keeping effect and slow-release performance, and the bioavailability of salicylic acid can be effectively improved, so that efficient protection on cold injury of fruits and vegetables is realized. The preparation process is simple, the production cost is low, and the application prospect is wide.

The present invention relates to the field of microbial technology, and more particularly to a strain of Saccharomyces cerevisiae and its application in producing ergosterol. The present invention screens and obtains a strain of Saccharomyces cerevisiae (Saccharomyces cerevisiae), and its deposit number is CCTCC M 20241572. The impurities of the ergosterol obtained by fermentation are few and high in purity, wherein the ergosterol content is more than 5% of the yeast dry weight, and the impurity 22,23-dihydroergosterol content is less than 1.5% of the yeast dry weight, which can meet the market demand for high-purity ergosterol products. Compared with traditional strains, the strain of the present invention can produce ergosterol with higher purity in high yield, and subsequent purification process can be simplified. The production process of the present invention is simple, low in cost, and more suitable for industrialized production.

The present application belongs to the field of bioengineering, and particularly relates to a kind of recombinant yeast and its construction method, the supernatant protein factor derived from southern Peromyscus, Homo sapiens and / or Gallus gallus is expressed in the recombinant yeast, squalene monooxygenase, phospholipid synthesis transcription factor, and / or product synthase (especially beta-amyrin synthase, ergosterol synthase and / or lanosterol synthase), the recombinant yeast can efficiently synthesize triterpenoid compounds (especially squalene, beta-amyrin) and / or sterol compounds (especially ergosterol and / or lanosterol).

The present invention relates to the field of pharmaceutical chemistry, and specifically to a method for preparing cholesterol, a derivative thereof, and an analog thereof. The derivative of cholesterol comprises, but is not limited to, 7-dehydrocholesterol, 25-hydroxycholesterol, 25-hydroxy-7-dehydrocholesterol, and ergosterol. In the present invention, a compound represented by formula I can be prepared by means of a microbial transformation using phytosterols as a raw material, followed by preparing cholesterol, the derivative thereof, and the analog thereof.

The invention is applicable to the technical field of drug detection, and provides a sterility test method of amphotericin B liposome for injection, which comprises the following steps: mixing a casein tryptone liquid culture medium with Tween, ergosterol and ethanol to obtain a culture medium containing a neutralizer, mixing a 0.1% peptone buffer solution with Tween, ergosterol and ethanol to obtain a culture medium containing a neutralizer, and carrying out freeze-drying to obtain the amphotericin B liposome for injection. A buffer solution containing the neutralizer is obtained; redissolving the amphotericin B lipidosome for injection by using sterilized water to obtain a lipidosome redissolved solution, diluting and uniformly mixing; filtering the diluted amphotericin B lipidosome for injection by adopting a membrane filtration method, washing the amphotericin B lipidosome for multiple times by using a buffer solution containing a neutralizing agent, and then adding a culture medium containing the neutralizing agent; and adding a standard test strain subjected to sterility test into the culture medium, and culturing. According to the method, the ergosterol is used as a neutralizing agent, the Tween is used as a dispersing agent, and the ethanol is used as a dissolving agent of the ergosterol, so that the effectiveness of the sterility test method of the amphotericin B liposome for injection is guaranteed.

Providing antioxidant compositions. [Solution] The present invention provides an antioxidant composition comprising an ergosterol analog or a koji mold fermentation product containing an ergosterol analog. The ergosterol analog is preferably a compound in an oxidized form of ergosterol. The ergosterol analog is preferably at least one selected from the group consisting of dehydroergosterol, 14-dehydroergosterol, 14,15-dehydroergosterol, and 24(28)-dehydroergosterol.