17 results about "Ergostanol" patented technology

The scheme provides a kind of engineered exosome double drug delivery system loaded with ergosterol and its preparation method and application, belongs to the field of nanomaterials and nanobiomedicine, based on the innovative ROS-responsive nanomaterial CHPG design, load the active ingredient ergosterol to play the role of treatment and repair, which realizes the blood-brain barrier penetration and precise drug release in lesion area through targeted functional modification;It is wrapped in the nasal mucosa-derived stem cell exosome to form a drug delivery system Exo@Erg-CHPG-M, which combines the natural biological characteristics of exosome and the precise targeting advantage of nanomicelle, significantly improves the drug delivery efficiency, in vitro and animal model verification show that, the system can improve the neurological impairment and brain tissue injury after ischemic stroke by synergistically regulating inflammation and neural repair pathways, the scheme provides a new technical scheme for the precise treatment of central nervous system diseases such as cerebral stroke.

The invention discloses a method for detecting the content of various components in a puffball sample and application of the method, and the method comprises the following steps: taking puffball sample powder, adding an extraction solvent for extraction, and preparing to obtain a test solution; taking alpha-linolenic acid, linoleic acid, palmitic acid, oleic acid, stearic acid and ergosterol reference substances, and adding a solvent to prepare a mixed reference substance solution; respectively injecting the mixed reference substance solution and the test solution into an ultra-high performance liquid chromatograph for detection, and comparing the maps of the reference substance solution and the test solution; and making a regression equation by using the sample size Ln value and the peak area Ln value of each component, and calculating the content of the components in the puffball sample. According to the quality detection method for the puffball sample, the contents of the six components can be simultaneously determined, the operation is simple, the accuracy is high, the recovery rate is reliable, the detection efficiency is high, and a basis is provided for standardizing the market of puffball medicinal materials and preparations and reasonably developing the puffball medicinal materials and preparations.

The invention provides application of ergosterol in preparation of a medicine for treating pulmonary arterial hypertension. The preparation method of the ergosterol comprises the following steps: cleaning, crushing and drying silkworm chrysalis cordyceps hainanensis sporocarp to obtain cordyceps militaris powder; the method comprises the following steps: mixing cholic acid, deoxycholic acid, methoxypolyethylene glycol and absolute ethyl alcohol to obtain ethanol mother liquor of a supramolecular solvent; carrying out gradient synergistic extraction on the cordyceps militaris extract; carrying out reduced pressure distillation and drying to obtain an extract, and carrying out chromatographic separation and drying; and recrystallizing, cleaning and drying to obtain the product. According to the invention, CO2 expanded ethanol liquid is adopted as a solvent, a cholic acid-deoxycholic acid-methoxypolyethylene glycol supramolecular solvent system technology and homologous adaptability of steroid nucleus structures of etocholic acid and deoxycholic acid and ergosterol are utilized to construct a multi-stage cavity recognition network, and C5-C6 double bonds and C22-C23 double bonds of ergosterol are specifically combined, so that the detection sensitivity of ergosterol is improved, and the detection sensitivity of ergosterol is improved. Meanwhile, the pore diameter of the cavity is regulated and controlled through methoxypolyethylene glycol, and the content of ergosterol in the extract is increased.

The application provides application of ergosterol in preparation of a medicine for treating pulmonary arterial hypertension. A preparation method of the ergosterol is as follows: Cordyceps militaris Haining strain fruiting bodies are taken, washed, crushed, and dried to obtain Cordyceps militaris powder; cholic acid, deoxycholic acid, polyethylene glycol monomethyl ether and anhydrous ethanol are mixed to obtain an ethanol mother liquor of a supramolecular solvent; Cordyceps militaris extraction liquid is gradiently and cooperatively extracted; vacuum distillation, drying, chromatographic separation, drying, recrystallization, washing and drying are sequentially performed, and the ergosterol is obtained. In the application, CO2 expanded ethanol liquid is used as a solvent, and a cholic acid-deoxycholic acid-polyethylene glycol monomethyl ether supramolecular solvent system technology is used. Relying on the homologous adaptability of the steroid nucleus structure of cholic acid and deoxycholic acid and ergosterol, a multi-level cavity recognition network is constructed, the C5-C6 double bond and the C22-C23 double bond sites of ergosterol are specifically combined, and the content of ergosterol in the extract is improved by regulating the cavity aperture through the polyethylene glycol monomethyl ether.

The present invention provides a yeast cell wall and a use thereof in the field of alcohol fermentation. The yeast cell wall is a yeast cell wall of Saccharomyces cerevisiae ER18 with the accession number of CCTCC NO: M 2020118. The yeast cell wall provided by the present invention is used as an encapsulation carrier of an alcohol fermentation promoter, and the encapsulation efficiency is improved by more than 30%. Moreover, the yeast cell wall is rich in ergosterol and other sterols and a plurality of unsaturated fatty acids, has a high nutritional value, and can provide a nutritional raw material for alcohol fermentation, thereby improving the yeast fermentation performance and improving the alcohol yield of alcohol fermentation.

The invention relates to the technical field of medicine synthesis, in particular to ergosterol amino alcohol derivatives with antibacterial activity and a preparation method and application thereof. The structure is as shown in general formula (I). The ergosterol amino alcohol derivative disclosed by the invention is high in gram-positive bacterium and gram-negative bacterium resisting activity, and the antibacterial activity of the derivative with the optimal activity on staphylococcus aureus and escherichia coli is improved by 64 times and 32 times respectively, so that the ergosterol amino alcohol derivative is obviously superior to that of lead compound ergosterol and compounds reported in literatures. The IC50 values of the human body normal cells HK-2 (near-end renal tubular epithelial cells) and HEK-293 (human embryo renal epithelial cells) are obviously higher, and when MIC < lt > is less than MIC < lt >, MIC < lt > is less than MIC < lt >; the ergosterol amine alcohol derivative has the selectivity index SI of more than or equal to 6.0 when the molecular weight of the ergosterol amine alcohol derivative is 32 [mu] g mL <-1 >, has the characteristics of high efficiency and low toxicity, and has potential application value in the aspect of anti-infection drug development.

The application of ceramide in the control of rice blast fungus falls within the field of biological control. This invention relates to the application of ceramide in the control of rice blast fungus: a combination of ceramide and lipopeptides is used for the control of rice blast fungus. Exogenous addition of ceramide significantly enhances the stress effect of lipopeptides on rice blast fungus. It also enhances the abnormal accumulation of glycogen and lipid droplets in conidia, promotes the bursting of ROS, and increases intracellular ceramide and calcium... 2+ The invention increases the content of malondialdehyde (MDA) in mycelium, enhances membrane lipid peroxidation, and decreases ergosterol content. Furthermore, it inhibits the expression of ergosterol synthesis genes, UPR target genes, and sphingolipid metabolism-related genes. This invention develops a highly efficient natural agent against rice blast by combining exogenous Cer with lipopeptides. This agent effectively reduces the pathogenicity of rice blast fungus and significantly inhibits its infection of rice, laying the foundation for the development of novel biological control agents.

The invention discloses an ergosterol derivative derived from spartina alterniflora and a preparation method and application of the ergosterol derivative. The ergosterol derivative has the following molecular structure expression formula, researches prove that the ergosterol derivative provided by the invention not only can reduce fasting blood glucose, but also has a good control effect on postprandial blood glucose, so that the ergosterol derivative shows an excellent overall blood glucose control effect and has a good clinical application prospect;

The invention relates to the field of yeast extraction, in particular to a method for co-producing ergosterol and yeast sterol from yeast. The invention provides a method for co-producing ergosterol and yeast sterol from yeast, 4-phenyl-3H-1, 2, 4-triazoline-3, 5-diketone can selectively react with ergosterol in a sterol mixed solution, and the yeast sterol does not participate in the reaction, so that the purposes of removing the ergosterol and purifying the yeast sterol are achieved. The co-production of the yeast sterol is realized from the yeast for producing the ergosterol, and the economic value is improved while the tailing discharge is reduced. Compared with an existing technology for extracting yeast sterol from yeast, the technology does not need multi-step chromatographic separation, the process is greatly simplified, the selectivity is high, and the purity of yeast sterol is high.

Disclosed herein is an improved and efficient process for synthesis of vitamin D3 and its analogue Calcifediol from Ergosterol. Particularly, the present invention discloses the synthesis of key intermediate 3β-tert-Butyldimethylsilyloxy-22-hydroxy-23,24-bisnorchola-5,7-diene (5), and novel intermediate β-tert-Butyldimethylsilyloxy-22-iodo-23,24-bisnorchola-5,7-diene (9) by a simple and cost effective process. The industrially viable processes for preparation of said intermediate(s) results in providing provitamins with various side chains and the desired products in high yield.

This invention relates to the field of pharmaceutical testing technology and provides a method for sterility testing of amphotericin B liposomes for injection, comprising the following steps: mixing tryptic soy peptone liquid culture medium with Tween, ergosterol, and ethanol to obtain a culture medium containing a neutralizing agent; mixing 0.1% peptone buffer with Tween, ergosterol, and ethanol to obtain a buffer solution containing a neutralizing agent; reconstituteing the amphotericin B liposomes for injection with sterile water to obtain a liposome reconstituted solution, and diluting and mixing it evenly; filtering the diluted amphotericin B liposomes for injection using a membrane filtration method, rinsing repeatedly with the buffer solution containing the neutralizing agent, and then adding the culture medium containing the neutralizing agent; adding a standard test strain for sterility testing to the culture medium and culturing. This invention uses ergosterol as a neutralizing agent, Tween as a dispersant, and ethanol as a solvent for ergosterol, ensuring the effectiveness of the sterility testing method for amphotericin B liposomes for injection.

The present application relates to the technical field of functional food preparation and development of resources of medicinal and edible origin, and specifically relates to a compound lipid-lowering functional tablet candy of coix seed and red koji and a preparation method thereof. The present application realizes the synergistic promotion of active ingredient enrichment, stable processing characteristics and efficient lipid-lowering effect through precise control of the solid-state fermentation process of coix seed by Monascus purpureus and formula optimization based on the AHP-CRITIC mixed weighting method. The content of lovastatin in the tablet candy of the present application is 1.35 times that of the same type of commercially available red koji product, the content of phytosterols (especially ergosterol) is more than 4 times that of commercially available products, and the product retains the coixol component that is not detected in commercially available products. The raw materials of the present application are all resources of medicinal and edible origin or food-grade auxiliary materials, and have high long-term safety. The tablet candy has high standardization, is easy to take and store stably, and meets the convenience needs of consumers for daily health management.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing ergosterol and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the method, sterol synthesis route genes are screened, the speed limiting step is determined, high-yield ergosterol is preliminarily achieved through combined expression, the flow direction of carbon metabolic flow is further pulled through lipid droplet engineering and cell area chamber engineering, and the yield of ergosterol is increased. On the basis of enzyme modification engineering, the catalytic efficiency of the ERG11 on the lanosterol is improved through substrate channel engineering and proton-dependent catalytic path construction. Through multi-copy integration of the genes in the speed limiting step, the yield of ergosterol in a shake flask of the constructed engineering strain reaches 433.1 mg / L, and the yield of ergosterol in a 5L fermentation tank system reaches 4.58 g / L.

The present application belongs to the field of bioengineering, and particularly relates to a kind of recombinant yeast and its construction method, the supernatant protein factor derived from southern Peromyscus, Homo sapiens and / or Gallus gallus is expressed in the recombinant yeast, squalene monooxygenase, phospholipid synthesis transcription factor, and / or product synthase (especially beta-amyrin synthase, ergosterol synthase and / or lanosterol synthase), the recombinant yeast can efficiently synthesize triterpenoid compounds (especially squalene, beta-amyrin) and / or sterol compounds (especially ergosterol and / or lanosterol).

Providing antioxidant compositions. [Solution] The present invention provides an antioxidant composition comprising an ergosterol analog or a koji mold fermentation product containing an ergosterol analog. The ergosterol analog is preferably a compound in an oxidized form of ergosterol. The ergosterol analog is preferably at least one selected from the group consisting of dehydroergosterol, 14-dehydroergosterol, 14,15-dehydroergosterol, and 24(28)-dehydroergosterol.

The present invention relates to genetically-modified oleaginous yeasts for producing non-native sterols at commercially useful levels, especially for example in providing sterols individually or as a mixture in an artificial dietary composition for honeybees or other insects or animals. For this purpose, an oleaginous yeast, e.g. Yarrowia lipolytica, may be employed wherein the yeast has reduced production of ergosterol compared with a wild-type oleaginous yeast or is incapable of producing ergosterol and is provided with a sterol surrogate to aid growth. From such yeast, however, other yeast may be engineered which retain useful sterol production without need for a sterol surrogate, e.g. production of sterol mixtures in which 24-methylenecholesterol or campesterol is the dominant sterol.

The application discloses a synthesis method of a renal fibrosis inhibitor from marine fungi and application of the renal fibrosis inhibitor in preparation of an anti-renal fibrosis drug, wherein the renal fibrosis inhibitor from marine fungi is a ergosterol-pyrone hybrid steroid compound with a structure as shown in formula I, and the method comprises the following steps: in a non-proton organic solvent, taking ergosterol and a pyrone derivative as substrates, performing one-step non-enzymatic coupling reaction in the presence of an acid promoter, and after the reaction is completed, performing separation and purification to obtain the renal fibrosis inhibitor from marine fungi, wherein the synthesis route is extremely simple, the yield is high, a production bottleneck is broken, and the obtained striasteroid B has significant inhibitory renal fibrosis activity and drug-making potential: I, wherein R is -CH3 or -CH2OH.