Method for analyzing and determining the content of antioxidant pep-36 by high performance liquid chromatography

A high-performance liquid chromatography, PEP-36 technology, applied in the field of high-performance liquid chromatography analysis and determination of antioxidant PEP-36 content, can solve problems such as the limit and detection method of unloaded antioxidant PEP-36, and achieve Strong specificity, good separation, and ensure the effect of quality control

Active Publication Date: 2015-08-05
SHANDONG YONGJU MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the European Pharmacopoeia, the limits and detection methods of commonly used antioxidants such as Antioxidant 1010, Antioxidant 330, Antioxidant 1076, Antioxidant 168, etc. are clearly discussed, but the antioxidant PEP-36 is not included Other pharmacopoeias and authoritative organizations at home and abroad have not included the limits and detection methods of the antioxidant PEP-36

Method used

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  • Method for analyzing and determining the content of antioxidant pep-36 by high performance liquid chromatography
  • Method for analyzing and determining the content of antioxidant pep-36 by high performance liquid chromatography
  • Method for analyzing and determining the content of antioxidant pep-36 by high performance liquid chromatography

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Effect test

Embodiment 1

[0032] Instruments and Conditions

[0033] Shimadzu 20A high performance liquid chromatography; column: Shimadzu InertsilODS-3, 250×4.6mm, 5μm; UV detection wavelength: 200nm; column with octadecylsilane bonded silica gel; mobile phase: acetonitrile- Tetrahydrofuran-water, the volume ratio of acetonitrile, tetrahydrofuran and water is 70:10:20; column temperature: 25°C; mobile phase flow rate: 0.8ml / min; injection volume: 10μl.

[0034] The volume ratio of toluene and methanol in toluene-methanol solvent is 1:1.

[0035] Experimental procedure

[0036] (1) Take 5g of the polypropylene interface and place it in a borosilicate glass bottle with a ground glass stopper. Add 50ml of toluene and boil under reflux for 1 hour;

[0037] (2) After reflux finishes, keep stirring and cooling and add 50ml methanol;

[0038] (3) filter through No. 4 sand core funnel, and filter again with the toluene-methanol solution washing flask, all filtrates are transferred in the 500ml measuring b...

Embodiment 2

[0043] Instruments and Conditions

[0044] Shimadzu 20A high performance liquid chromatography; column: Shimadzu InertsilODS-3, 250×4.6mm, 5μm; UV detection wavelength: 240nm; column with octadecylsilane bonded silica gel; mobile phase: acetonitrile- Tetrahydrofuran-water, the volume ratio of acetonitrile, tetrahydrofuran and water is 70:15:15; column temperature: 30°C; flow rate of mobile phase: 0.9ml / min; injection volume: 15μl.

[0045] The volume ratio of toluene and methanol in toluene-methanol solvent is 1:1.

[0046] Experimental procedure

[0047] (1) Take 7g of polypropylene film and place it in a borosilicate glass bottle with a ground glass stopper. Add 70ml of toluene and boil under reflux for 1.5 hours;

[0048] (2) After reflux finishes, keep stirring and cooling and add 70ml methanol;

[0049] (3) filter through No. 4 sand core funnel, and filter again with the toluene-methanol solution washing flask, all filtrates are transferred in the 500ml measuring bott...

Embodiment 3

[0054] Instruments and Conditions

[0055] Shimadzu 20A high performance liquid chromatography; column: Shimadzu InertsilODS-3, 250×4.6mm, 5μm; UV detection wavelength: 270nm; column with octadecylsilane bonded silica gel; mobile phase: acetonitrile- Tetrahydrofuran-water, the volume ratio of acetonitrile, tetrahydrofuran and water is 70:15:15; column temperature: 35°C; mobile phase flow rate: 1.1ml / min; injection volume: 15μl.

[0056] The volume ratio of toluene and methanol in toluene-methanol solvent is 1:1.

[0057] Experimental procedure

[0058] (1) Take 8g of polypropylene combination cap and place it in a borosilicate glass bottle with a ground glass stopper. Add 80ml of toluene and boil under reflux for 2 hours;

[0059] (2) After reflux finishes, keep stirring and cooling and add 80ml methanol;

[0060] (3) filter through No. 4 sand core funnel, and filter again with the toluene-methanol solution washing flask, all filtrates are transferred in the 500ml measurin...

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PUM

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Abstract

The invention belongs to the field of the analysis of a compound, and relates to a method for analyzing and determining the content of an antioxidant PEP-36 through high performance liquid chromatography. The condition of the liquid chromatography is as follows: a chromatographic column adopts octadecyl silane bonded silica gel as a filler, and acetonitrile-tetrahydrofuran-water mixed solvent is used as a flow phase, wherein the volume ratio of acetonitrile to tetrahydrofuran to water is 70 to 10 to 20 or 70 to 20 to 10. The method is good in separation degree and strong in specificity; each peak can be completely separated from the adjacent impurity peak, the all peaks can be completely separated from each other, the content of the antioxidant PEP-36 can be accurately determined, and the quality control of polypropylene internal packing material for the injection with antioxidant PEP-36 can be guaranteed.

Description

technical field [0001] The invention relates to the field of compound analysis, in particular to a method for analyzing and measuring the content of antioxidant PEP-36 by high performance liquid chromatography. Background technique [0002] Polypropylene materials will age due to heat, light, electric field, radiation, metal ions or chemical media during the process of molding, storage and use, resulting in significant changes in physical properties such as impact strength, tensile strength and elongation. Therefore, antioxidants are usually added in the production process to improve, inhibit or delay its aging degradation process. [0003] Currently commonly used antioxidants include antioxidant 1010, antioxidant 330, antioxidant 1076, antioxidant 168 and antioxidant PEP-36, etc., which can improve, inhibit or delay the aging and degradation of polypropylene materials. , may migrate into injections. In order to control its content, the limits and detection methods of sever...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/36
Inventor 李兴春路杰张建勇宋俊梅李来玲耿会力李洁
Owner SHANDONG YONGJU MEDICAL TECH
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