Analytical method of gonyautoxins detected by combination of molecular imprinting solid phase extraction-hygroplasm

Abstract

The invention discloses an analytical method of gonyautoxins detected by combination of molecular imprinting solid phase extraction-hygroplasm, and belongs to the field of front treatment and chemical analysis and detection of a biological sample. The method applies a molecular imprinting polymer to structure a three-dimensional cavity and a specific recognition site which are highly similar as the imprinting molecule, so as to realize the selective recognition of the imprinting molecule, and improve efficiency of gathering target objects and removing interferent; moreover, an analytical method of hygroplasm combination detected target taking cyan column as a chromatograph column is developed; the cyan column has strong polarity in use of reverse phase, thus polarity target objects and impurities in the sample can be effectively separated; then better separating effect and mass spectrometric detection effect of gonyautoxins on the chromatographic column can be obtained according to the optimized hygroplasm condition. The method is featured by small sample demand, high recycle rate and precision, and low detection limit; the matrix effects in detection are reduced; the analytical method is applicable to the extraction and detection of multiple shellfish samples, and has very good universality.

Description

technical field [0001] The invention belongs to the field of biological sample pretreatment and chemical analysis and detection, and relates to a molecular imprinted solid-phase extraction-liquid-mass spectrometry analysis method for detecting gonyellin. Background technique [0002] GTX2&3 is a neurotoxin, which belongs to the paralytic shellfishpoisoning toxins (PSP), and is mainly secreted by marine phytoplankton (Alexandrium, Gymnodinium). In recent years, due to marine environmental pollution, toxic red tides have frequently broken out in the waters near my country. The algae toxins secreted by them can be passed through the food chain and enriched by shellfish to cause human poisoning, which seriously endangers the development of marine fisheries and human health. [0003] Aiming at the hazards of paralytic shellfish poisoning, it is imperative to establish and improve accurate detection methods and improve detection and prevention capabilities. Now the widely recogniz...

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Problems solved by technology

[0005] In order to solve the problem of accurate qualitative and quantitative analysis of algae toxins by biological analysis methods (mouse biological method, enzyme-linked immunoassay) and liquid chromatography-fluorescence detection methods in the prior art, and the solid-phase extraction-liquid chromatography-mass spectrometry detection method Problems with high detection limits and significant matrix effects

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