The invention discloses an HPLC analysis method of key intermediate impurities for dipyridamole synthesis. In the HPLC analysis method, a chromatographic column uses phenylsilane-bonded silica gel asa filler, an organic phase containing an acidic additive is used as a mobile phase A, an aqueous solution containing the acidic additive is used as a mobile phase B, a gradient elution method is adopted, the detection wavelength is 210-230 nm, and a key intermediate of the dipyridamole, namely 2,6-dichloro-4, 8-di(piperidin-1-yl)pyrimido[5,4-d] pyrimidine, and related impurities thereof are separated and determinated. The HPLC analysis method has the characteristics of a good degree of separation, simpleness, rapidity, high specificity and high sensitivity; by the HPLC analysis method, the quality of the key intermediate dichloride during the dipyridamole synthesis can be controlled, so that the post-treatment is simplified and the quality of a final product is guaranteed.