Multi-component content determination method of Miao medicine Laportea bulbifera

A determination method, the technology of red hemp, which is applied in the field of medicine, can solve the problems of lack of quality standards, achieve the effects of low specific detection limit and quantification limit, effective analysis and simple method

Inactive Publication Date: 2019-01-25
GUIZHOU MEDICAL UNIV
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Problems solved by technology

[0003] This standard only describes its properties, properties, taste, functions and indications, usage, dosage and storage conditions, but there are few reports on the content determination of its components, and there is a lack of perfect quality standards

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  • Multi-component content determination method of Miao medicine Laportea bulbifera

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[0018] In order to make the objectives, technical solutions, and advantages of the present invention clearer, the following further describes the present invention in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, but not to limit the present invention.

[0019] In the description of the present invention, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientation or positional relationship indicated by "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description. It does not indicate or imply that the pointed device or element must have a specific orientation, be constructed and operated in a speci...

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Abstract

The invention discloses a multi-component content determination method of Miao medicine Laportea bulbifera. The method comprises the following steps: S100, preparation of mixed reference solution, respectively and accurately weighing right amount of reference substances of Gallocatechin, neochlorogenic acid, Epigallocatechin, catechinic acid, chlorogenic acid, Cyclohexanecarboxylic acid, epicatechin, rutin, isoquercitrin, kaempferol-3-0-rutinoside and quercitrin; and simultaneously determining contents of eleven components including the Gallocatechin, the neochlorogenic acid, the Epigallocatechin, the catechinic acid, the chlorogenic acid, the Cyclohexanecarboxylic acid, the epicatechin, the rutin, the isoquercitrin, the kaempferol-3-0-rutinoside and the quercitrin. The method is simple, efficient, highly sensitive and excellent in specificity, and can provide baisis for quality control of Laportea bulbifera medicinal materials. Compared with the ordinary liquid phase, the method has the advantages of being accurate, reliable and highly sensitive, and having lower specificity detection limit and quantification limit, and is more effective in medicinal material component content analysis through a liquid chromatography-mass spectrometry analysis technology.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for measuring the content of multiple components of Miao medicine red hemp. Background technique [0002] Miao medicine red grass is the fresh or dried whole plant of urtica plant wormwood wormwood, also known as wild green hemp, which is a common medicinal material of Guizhou Miao medicine. It has the effects of dispelling wind and dampness, promoting blood circulation and removing blood stasis. It is used for the treatment of rheumatic numbness, falls, and fractures. The medicinal materials are included in the "Quality Standards for Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province" (2003 edition). [0003] The standard only describes its properties, tastes, functions and indications, usage and dosage, and storage conditions, while the content determination of its components is rarely reported, and there is a lack of complete qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 陈思颖吴耽唐娟巩仔鹏王爱民李维维王永林兰燕宇李勇军李靖李莹刘亭孙佳李月婷杨畅陆苑潘洁
Owner GUIZHOU MEDICAL UNIV
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