24 results about "Gallocatechin" patented technology

The invention relates to preparation and application of a malignant tumor targeting gambogic acid preparation. An inner core-middle layer-shell three-layer structure is adopted, wherein the inner core is a gambogic acid-black phosphorus quantum dot compound and has chemical treatment and photo-thermal treatment functions; the middle layer is nano vesicles derived from frozen NK92 MI cells and provides natural immune targeting and killing ability; a shell is a hyaluronic acid chelate, and active targeting and pH / photo-thermal response type drug controlled release are achieved. The core of the preparation method is that a coaxial electrojet technology is adopted, three layers of materials are formed in one step, the process is efficient, and the preparation stability is good. The preparation can realize photo-thermal-chemical-immune triple synergistic treatment, has near-infrared fluorescence and photo-acoustic multimode imaging functions, effectively solves the problems of poor targeting property, large toxic and side effects and lack of real-time monitoring of traditional chemotherapy, and shows significant advantages and application potential in precise treatment and diagnosis of solid tumors.

The invention discloses composite hydrogel based on hyaluronic acid as well as a preparation method and application of the composite hydrogel, and belongs to the technical field of hydrogel. In order to deal with ROS (reactive oxygen species) amplified at a radiation injury part and regulate and control an inflammatory environment at a wound part, natural polyphenol epigallocatechin gallic acid (EGCG) with outstanding oxidation resistance is introduced into a system, and the composite hydrogel based on hyaluronic acid is synthesized.

The invention relates to the technical field of taste substance marker combinations, in particular to a taste substance marker combination for identifying flavor and an application method of the taste substance marker combination. The content of quercetin, epigallocatechin, 3-p-coumaroylquinic acid (3-O-p-coumaroylquinic acid) and 4-p-coumaroylquinic acid (4-p-coumaroylquinic acid) in the areca-flavor Liupao tea is remarkably higher than that of aged aroma type Liupao tea, and the content of 3-hydroxybenzoic acid (3-hydroxybenzoic acid) in the areca-flavor Liupao tea is remarkably lower than that of the aged aroma type Liupao tea. According to the present invention, the taste substances of different aroma type tea leaf samples of the Liupao tea are analyzed by using the liquid chromatography-mass spectrometry instrument analysis method, the key taste difference compounds capable of distinguishing the aged aroma flavor and the areca-nut aroma flavor of the Liupao tea are screened out, and the taste difference of the Liupao tea can be distinguished through the discrimination model (such as principal component analysis PCA, partial least squares discriminant analysis PLS-DA and the like); and determining the flavor type according to an output judgment result.

Methods for selectively preparing monoglucosides of epigallocatechin and epigallocatechin gallate are provided, and the use of these products in cosmetics.

Stabilized oils including an edible oil and an antioxidant composition comprising α-lipoic acid and least one of ascorbic acid, ascorbyl palmitate, green tea extract, lecithin, and rosemary extract or at least one of 1,2,4-benzenetriol, carnosic acid, dihydromyricetin, dihydrorobinetin, epigallocatechin, gallic acid, 3-hydroxytyrosol, myricetin, and nepodin, and methods of preparing such stabilized edible oils. The stabilized oils may have an Oxidative Stability Index (“OSI”) at 110° C. of at least 30 hours.

This invention relates to the field of fruit preservation technology and discloses a method for preserving mangoes containing chitosan, a eutectic solvent, and EGC. The method includes the following steps: adding chitosan to an acetic acid solution and heating and stirring in a water bath to obtain a chitosan film solution; pre-dispersing and wetting epigallocatechin in a eutectic solvent, then adding it to the aforementioned chitosan film solution and stirring at room temperature to obtain a modified chitosan composite film solution; selecting undamaged mangoes, washing and air-drying their surface moisture, immersing them in the modified chitosan composite film solution to evenly coat the surface with the film solution, removing them and air-drying them naturally, repeating the aforementioned immersion and air-drying process, and finally storing the dried mangoes in an indoor environment for preservation. This invention utilizes a eutectic solvent to solve the problem of easy aggregation of polyphenolic active substances, forming a uniform and continuous semi-permeable membrane on the mango peel, limiting moisture loss, achieving slow release of antioxidant components, and significantly extending the mango's shelf life.

The present disclosure relates essentially to a supplement, the function of which is to enhance immunity, prevent various diseases and assist in therapeutic treatments of various diseases, said supplement includes: 100 mg of lipoic acid, 1 mg of astaxanthin, 10 mg of glutathione, 5 mg of resveratrol, 5 mg of lycopene, 50 mg of epigallocatechin, 300 mg of curcumin, 400 mg of quercetin, 10 mg of coenzyme Q1 and 100 mg of dry açaí extract; formulated with the intention of combining substances that serve functions of assisting in the treatment of various diseases, such as: lipoic acid, which helps in the prevention of liver damage from intoxication, and can be used as a coadjuvant for the treatment of liver cirrhosis due to alcoholism and a coadjuvant for the treatment of diabetes and cataracts.

The disclosure relates to crystalline Form X of epigallocatetchin-3-gallate, compositions containing Form X, and methods of using Form X. The disclosure also relates to methods of making crystalline Form X of epigallocatetchin-3-gallate.

The invention discloses an aleurites montana anthocyanin reductase VmANR gene and application thereof. The amino acid sequence of the aleurites montana anthocyanin reductase VmANR gene is shown as SEQ ID NO. 2. The content of compounds such as catechin, (-)-epicatechin, (-)-epigallocatechin, phlorizin and dihydromyricetin in transgenic hairy root plants over-expressed by the VmANR obtained by a transgenic means is remarkably increased, so that more flavonoid compounds can be extracted, separated and purified, and greater economic benefits are created; the method provides an important theoretical basis for increasing the content of flavonoid compounds in plants by using a biotechnological means in the future, and has a relatively high application value.

The invention relates to self-degradable environment-friendly pet deodorization particles as well as a preparation method and application thereof, and relates to the technical field of pet deodorization. The deodorization particles for the pets are prepared from the following raw materials in parts by weight: 3 to 10 parts of chitosan-starch based modified materials, 1 to 3.5 parts of yucca plant extracts, 0.3 to 1.2 parts of gallocatechin, 0.45 to 2.4 parts of ellagic acid, 0.02 to 0.08 part of imine type COFs (Covalent Organic Frameworks) TpPa-1 and 0.02 to 0.05 part of gamma-terpineol. The self-degradable environment-friendly pet deodorization particle has good degradability and excellent deodorization and bacteriostasis effects, and the performance is improved through the synergistic effect of all the raw materials.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly provides a construction method and a content determination method of specific chromatograms of a Guangxi kadsura pepper stem aqueous extract and a preparation thereof, and the method comprises the following steps: (1) preparing a reference substance solution; (2) preparing a test solution; (3) detecting by adopting an ultra-high performance liquid chromatography to obtain seven or more common characteristic peaks, and realizing effective separation of the characteristic peaks containing gallic acid, protocatechuic acid, gallocatechin, protocatechualdehyde, catechin and epicatechin, so that the peak shape is good, and the base line is stable; and (4) taking catechin as an internal reference substance, establishing relative correction factors of protocatechuic acid, epicatechin and catechin, and establishing a content determination method of quantitative analysis of multi-components by single marker of three components. According to the method constructed by the invention, the content of the characteristic chromatogram and the content of the characteristic components can be simultaneously detected, the measured components can be completely separated, the detection time is short, and a basis is provided for quality detection and control of the Guangxi kadsura pepper stem aqueous extract and preparations thereof.

There has been need for tea beverages that maintain characteristics thereof while maintaining drinkability even with a low pH. One aspect of the present invention provides a beverage containing diacetyl and catechins, wherein the diacetyl content is at least 20 ppb and less than 10,000 ppb, the contained amount one or more types of catechins selected from epicatechin, catechin, epigallocatechin, gallocatechin, epicatechin gallate, catechin gallate, epigallocatechin gallate, and gallocatechin gallate is at least 1 ppm and less than 500 ppm, and the pH is 5 or lower.

The invention discloses a preparation method and application of a plant extract fermentation product for promoting anti-aging of cats, and belongs to the technical field of animal health care product preparation. Comprising a metabolite (LA20FM) of a brown algae extract which has an anti-aging function and is fermented by bifidobacterium adolescentis LifeAge20 and other raw materials, including one or more of D-sphingosine, luteolin, epigallocatechin, syringic acid, leucine, fucosterol, resveratrol, nicotinamide and glutathione. The metabolite of the brown algae extract fermented by the bifidobacterium adolescentis LifeAge20 disclosed by the invention has a remarkable effect in the anti-aging aspect, and can improve the content of nicotinamide adenine dinucleotide in vascular endothelial cells of cats, the angiogenesis capability and the antioxidant capability; the activity of beta-galactosidase in cells and the content of senescence phenotype markers are reduced, and a multi-channel anti-aging effect is realized. The LA20FM and additives are added to prepare the health-care agent, so that the health-care agent is more beneficial to anti-aging of pets and has a wide application prospect.

The invention discloses a method for selectively producing EGCG-4 '-O-alpha-D glucopyranoside by using sucrose phosphorylase, and belongs to the technical field of biological catalytic synthesis. An engineered mutant derived from leuconostoc mesenteroides sucrose phosphorylase (LmSPase) is used as a catalyst. The mutant is constructed through single-point or combined mutation on the basis of a wild type with the sequence of SEQ ID NO.1, and the enzyme activity, the stability and the substrate specificity of the mutant are all remarkably improved. By utilizing the high-performance mutant, a method for efficiently synthesizing (-)-epigallocatechin gallate 4 '-O-alpha-D glucopyranoside is established. The method is mild in reaction condition, simple and convenient to operate, short in reaction time and high in catalytic efficiency, and shows good application potential.

The application discloses a tea characteristic component composition for reducing acrylamide in Maillard reaction, and the composition is prepared by compounding at least three of five substances, i.e., epicatechin (EC), gallocatechin (GC), catechin gallate (CG), theabrine and theaflavine, and the compounded composition has the effect of reducing acrylamide, a by-product of Maillard reaction in food. In the composition of the application, the composition composed of epicatechin (EC), gallocatechin (GC), catechin gallate (CG) and theabrine has the best effect of reducing acrylamide, a by-product of Maillard reaction, and the reduction rate of acrylamide in a simulation system can be up to 75%. The composition of the application can be applied to baked food or fried food, especially baked food such as bread, cookie, potato chip and the like.

This invention provides a method for synthesizing novel natural pigments using phenotypic catechins. Various epicatechins, epigallocatechins, epicatechin gallate esters, and epigallocatechin gallate esters are used as substrates. These are mixed and subjected to a non-enzymatic oxidation reaction under humid and hot conditions to generate a new class of tea pigment compounds. The non-enzymatic oxidation products of the phenotypic catechins are then concentrated under reduced pressure, freeze-dried, extracted, and purified by column chromatography to obtain dimeric catechin pigments. The six novel dimeric catechin pigments obtained by this invention are synthesized using a simple method. The six novel dimeric catechin pigments obtained according to the method of this invention are orange-yellow or orange-red amorphous powders with good color-adjusting capabilities, and can be applied to coloring tea beverages and new-style tea drinks. The discovery of this type of natural pigment contributes to the research on coloring substances during the thermal processing of tea, the improvement of tea liquor color quality, and the development and application in the field of food coloring.

The invention discloses a sucrose phosphorylase mutant and application of the sucrose phosphorylase mutant in synthesis of epigallocatechin gallate glucoside, and belongs to the technical field of enzyme engineering and biological catalysis. The sucrose phosphorylase mutant is obtained by mutating a plurality of amino acid sites on the basis of an amino acid sequence of SEQ ID NO.1. The mutant not only remarkably improves the enzyme activity, but also more importantly changes the regioselectivity of the mutant; by utilizing the mutant, efficient catalytic synthesis of (-)-epigallocatechin gallate 4 ''-O-alpha-D glucopyranoside is realized, and a key foundation is laid for stable industrial production and large-scale application.

The invention provides a metal polyphenol network modified probiotic viable microneedle patch as well as a preparation method and application thereof, and the preparation method comprises the following steps: self-assembling doxorubicin DOX, ferric ion Fe < 3 + > and epigallocatechin gallate EGCG through a one-pot coordination reaction to form an antibiotic loaded metal-polyphenol network DM; modifying the surface of the DM by using polylysine PLL to obtain DMP with positive charges; dMP is evenly attached to the surface of lactobacillus reuteri Lr through electrostatic interaction, and DMP-coated Lr is obtained; and loading the DMP-coated Lr into a soluble microneedle matrix which takes polyvinyl alcohol (PVA) as a main body, so as to prepare the DMP-coated Lr microneedle patch. According to the invention, the problems of insufficient competitiveness and limited biological membrane penetration of the existing probiotics in infectious wound treatment are solved, and a treatment strategy with barrier penetration, early-stage powerful sterilization, long-term micro-ecological regulation and wound repair functions is provided.

The invention relates to the technical field of medical materials, in particular to a composite material for promoting bone tissue regeneration and a preparation method thereof. The preparation method comprises the following steps: firstly, using methacrylated gelatin as a raw material, using the methacrylated gelatin as a bone tissue repair main material, introducing a modified carbon material into the methacrylated gelatin, and introducing modified chondroitin molecules and epigallocatechin with antibacterial performance to the surface of the methacrylated gelatin, so that the methacrylated gelatin has high hydrophilic and high antibacterial functions; and in order to further enhance the biological activity of the composite material and promote the bone tissue regeneration function, exosome is introduced into the composite material as a bioactive substance to promote healing, so that the prepared composite material can cope with most bone repair scenes.

The invention provides a directional preparation method of epigallocatechin palmitate based on hydroxylamine deprotection. The preparation method comprises the following steps: carrying out acylation protection on ortho-phenolic hydroxyl of epigallocatechin, directionally esterifying a protection product, adding ethanol to remove excessive acyl chloride, adding a hydroxylamine reagent to selectively remove propionyl protecting groups, washing with petroleum ether to remove small polar molecular impurities, and drying to obtain the epigallocatechin. And the high-purity target product epigallocatechin palmitate can be obtained. The method has the advantages of simplicity and convenience in operation, simple post-treatment (without column chromatography purification), high product purity and the like.

The application provides a lipid-lowering sugar-free white tea probiotic fermentation liquor and a preparation method and application thereof, and belongs to the technical field of functional food processing. The method comprises the following steps: (1) white tea is crushed and sieved, then sterile distilled water is added, water extraction is performed in a boiling water bath, and finally cooling is performed; (2) probiotic powder is inoculated into the white tea culture medium obtained in step (1) for fermentation. The fermentation method provided by the application can effectively enrich flavonoids and polyphenolic compounds in the white tea water extract without adding glucose, and improve the content of various functional active ingredients such as hesperetin, quercetin, epigallocatechin and cinnamic acid. The sugar-free fermented white tea prepared by the application has a significantly improved flavonoid content compared with the sugar-containing white tea. The fermented white tea significantly reduces the oil and TC content of high-fat HepG2 cells, significantly improves the antioxidant activity, and reduces the expression amount of inflammatory factors. The application can provide ideas for further development of lipid-lowering functional foods mainly using white tea as a raw material, and has good application prospect.

Provided is a heat-sterilized beverage in which off-flavor due to heating is reduced while containing vegetable oil or fat. This heat-sterilized beverage contains vegetable oil or fat, wherein: epigallocatechin and an inorganic salt including a magnesium salt and / or a potassium salt are blended; the epigallocatechin content in the beverage is 0.1-20.0 mg per 100 ml; the magnesium content in the beverage is 0.4-15.0 mg per 100 ml when the inorganic salt included in the beverage includes a magnesium salt; and the potassium content in the beverage is 5-160 mg per 100 ml when the inorganic salt included in the beverage includes a potassium salt.

The invention discloses a preparation method and application of a plant extract fermentation product for promoting aging resistance of dogs, and belongs to the technical field of animal health care product preparation. Comprising a metabolite (LA20k9) of a brown algae extract which has an anti-aging function and is fermented by bifidobacterium adolescentis LifeAge20-k9 and other raw materials, and the metabolite is prepared from one or more of avenine, fucosterol, 3 ', 4', 5, 7-tetrahydroxyflavone, ciprofibrate, nicotinamide, epigallocatechin, leucine, syringic acid and glutathione. The metabolite of the brown algae extract fermented by the bifidobacterium adolescentis LifeAge20-k9 disclosed by the invention has a remarkable effect in the anti-aging aspect, and can improve the content of nicotinamide adenine dinucleotide in vascular endothelial cells of dogs, the angiogenesis capability and the antioxidant capability; the activity of beta-galactosidase in cells and the content of senescence phenotype markers are reduced, and a multi-channel anti-aging effect is realized. LA20k9 and additives are added to prepare the health-care agent, so that the health-care agent is more beneficial to aging resistance of pets and has a wide application prospect.