38 results about "Gallocatechin" patented technology

The invention belongs to the technical field of medicine manufacturing, and particularly relates to a compound preparation containing faropenem sodium as well as a preparation method and application of the compound preparation. According to the invention, the production prescription is optimized, the stability of the medicine can be effectively improved, the unpleasant smell of the medicine is covered, and the medication compliance of children is obviously improved. The epigallocatechin-aluminum complex can serve as a free radical scavenger in a preparation system, oxygen free radicals are effectively captured and neutralized, and the influence of oxidative stress on the medicine is reduced; moreover, the existence of aluminum ions not only enhances the stability of EGCG, but also reduces the drug degradation reaction under the catalysis of the ions through chelation with trace metal ions (such as iron, copper and the like) in the preparation. In addition, aluminum ions can also directly form a stable complex with faropenem sodium, so that the chemical stability of the faropenem sodium is further improved.

The present invention discloses a method for improving the quality of Millettia reticulata, belonging to the technical field of Millettia reticulata cultivation. It applies a growth regulator in combination with spectral lighting. The growth regulator is methyl jasmonate, with a concentration of 100 - 400 μmol / L. The LED light quality of the spectral lighting is red light and blue light, and the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the light quality ratio of red light to blue light is 2 - 5:1. The method of the present invention can effectively improve the growth of Millettia reticulata seedlings, significantly increase the contents of the active ingredients total flavonoids, catechins, epicatechins and gallocatechins, especially the improvement effect of catechins is the best. In an embodiment, compared with the blank group, the contents of total flavonoids, catechins, epicatechins and gallocatechins are significantly increased by 83.05%, 314.72%, 81.03% and 179.62% respectively, and the effect is remarkable.

The invention provides a detection method of flavan-3-alcohol sulfonate and application of the detection method. The method comprises the following specific steps: preparing and separating flavane-3-alcohol sulfonate, analyzing and screening molecular characteristic correlation and mass spectrum signal response, identifying the structure of flavane-3-alcohol sulfonate, drawing a standard curve of a flavane-3-alcohol sulfonate standard product, and detecting and analyzing flavane-3-alcohol sulfonate in wine. According to the invention, the epicatechin-(4beta)-sulfonate and the epigallocatechin-(4beta)-sulfonate are obtained by inserting sulfonyl into catechin compounds for preparation and separation, and the epicatechin-(4beta)-sulfonate and the epigallocatechin-(4beta)-sulfonate are used as standard substances for detecting the flavan-3-alcohol sulfonate in the grape wine and are applied to detection and analysis of the flavan-3-alcohol sulfonate in the grape wine. The method provided by the invention has the characteristics of simple and rapid compound synthesis route, easy control of operation conditions, high identification accuracy and sensitivity and strong specificity, provides a good method reference for detection of flavan-3-alcohol sulfonate in the wine aging process, and ensures the fragrant and mellow flavor and taste of wine.

The invention discloses an intestinal tract hemostatic composition as well as a preparation method and application thereof, and relates to the field of hemostatic drugs. Wherein the catechin, the hyperoside, the isoquercitrin, the quercetin-3-O-glucuronide, the gallocatechin, the myricetin-3-O-galactoside, the proanthocyanidin B1, the proanthocyanidin B3, the syringe-3-O-glucoside and the astragalus smicus glycoside are flavonoid compounds; nuciferine, higenamine, N-methyl-higenamine and N-trans-feruloyl tyramine are taken as alkaloid compounds, and the compound is selected from the group consisting of higenamine, nuciferine, higenamine The composition has the advantages of simple preparation process, good hemostatic effect and the like, and provides a new raw material choice for the development of intestinal bleeding treatment medicines.

The invention relates to preparation and application of a malignant tumor targeting gambogic acid preparation. An inner core-middle layer-shell three-layer structure is adopted, wherein the inner core is a gambogic acid-black phosphorus quantum dot compound and has chemical treatment and photo-thermal treatment functions; the middle layer is nano vesicles derived from frozen NK92 MI cells and provides natural immune targeting and killing ability; a shell is a hyaluronic acid chelate, and active targeting and pH / photo-thermal response type drug controlled release are achieved. The core of the preparation method is that a coaxial electrojet technology is adopted, three layers of materials are formed in one step, the process is efficient, and the preparation stability is good. The preparation can realize photo-thermal-chemical-immune triple synergistic treatment, has near-infrared fluorescence and photo-acoustic multimode imaging functions, effectively solves the problems of poor targeting property, large toxic and side effects and lack of real-time monitoring of traditional chemotherapy, and shows significant advantages and application potential in precise treatment and diagnosis of solid tumors.

The invention discloses composite hydrogel based on hyaluronic acid as well as a preparation method and application of the composite hydrogel, and belongs to the technical field of hydrogel. In order to deal with ROS (reactive oxygen species) amplified at a radiation injury part and regulate and control an inflammatory environment at a wound part, natural polyphenol epigallocatechin gallic acid (EGCG) with outstanding oxidation resistance is introduced into a system, and the composite hydrogel based on hyaluronic acid is synthesized.

Methods for selectively preparing the monoglucosides of epigallocatechin and epigallocatechin gallate, and the use of these products in cosmetics, are provided.

The invention relates to the technical field of taste substance marker combinations, in particular to a taste substance marker combination for identifying flavor and an application method of the taste substance marker combination. The content of quercetin, epigallocatechin, 3-p-coumaroylquinic acid (3-O-p-coumaroylquinic acid) and 4-p-coumaroylquinic acid (4-p-coumaroylquinic acid) in the areca-flavor Liupao tea is remarkably higher than that of aged aroma type Liupao tea, and the content of 3-hydroxybenzoic acid (3-hydroxybenzoic acid) in the areca-flavor Liupao tea is remarkably lower than that of the aged aroma type Liupao tea. According to the present invention, the taste substances of different aroma type tea leaf samples of the Liupao tea are analyzed by using the liquid chromatography-mass spectrometry instrument analysis method, the key taste difference compounds capable of distinguishing the aged aroma flavor and the areca-nut aroma flavor of the Liupao tea are screened out, and the taste difference of the Liupao tea can be distinguished through the discrimination model (such as principal component analysis PCA, partial least squares discriminant analysis PLS-DA and the like); and determining the flavor type according to an output judgment result.

The invention discloses application of epigallocatechin gallate in relieving formation of a hard core of salted egg yolk and improving the quality of the salted egg yolk, belongs to the field of food agriculture, and particularly discloses pickling liquid and pickling mud added with the epigallocatechin gallate and application of the pickling liquid and the pickling mud in poultry egg pickling. The epigallocatechin gallate is used as a food-borne antioxidant, and the epigallocatechin gallate is added into the pickling material to relieve the formation of the hard core of the salted egg yolk so as to improve the quality of the salted egg yolk. The salted egg pickling material is simple and easy to obtain, the hard heart rate and the hard heart mass ratio of the salted egg yolk are reduced and the hardness and the sandy property of the salted egg yolk are improved by adding the epigallocatechin gallate into the salty water or salty mud pickling material, and the invention aims to disclose a new application of the epigallocatechin gallate in salting the salted egg.

Methods for selectively preparing monoglucosides of epigallocatechin and epigallocatechin gallate are provided, and the use of these products in cosmetics.

Stabilized oils including an edible oil and an antioxidant composition comprising α-lipoic acid and least one of ascorbic acid, ascorbyl palmitate, green tea extract, lecithin, and rosemary extract or at least one of 1,2,4-benzenetriol, carnosic acid, dihydromyricetin, dihydrorobinetin, epigallocatechin, gallic acid, 3-hydroxytyrosol, myricetin, and nepodin, and methods of preparing such stabilized edible oils. The stabilized oils may have an Oxidative Stability Index (“OSI”) at 110° C. of at least 30 hours.

The application discloses a kind of double ester type catechins compound and preparation method, steps are as follows: tea is mixed with methanol aqueous solution, and tea extract is obtained by ultrasonic extraction;Tea extract is continuously carried out reverse phase liquid chromatography separation, and finally double ester type catechins compound is obtained by collecting specific eluent and the distillate of specific time period, which is obtained epigallocatechin-3,4'-di- O -gallate and gallocatechin-3,4'-di- O -gallate is first purified by separation, and double ester type catechins compound has high purity.

The application belongs to the technical field of natural fruit active substance detection, and discloses a method for simultaneously detecting the contents of multiple active components in green fruits based on HPLC, wherein the active components include seven main active components in green fruits, i.e., pyrogallol, gallic acid, epicatechin, ethyl gallate, p-coumaric acid, epigallocatechin and ellagic acid. The detection method established by the application is good and the instrument is precise, and the method has the technical advantages of good selectivity, high sensitivity and accuracy and reliability for detecting the contents of the main active components in green fruits, thereby providing a new analysis method and reliable basis for quality analysis and quality control of green fruits.

This invention relates to the field of fruit preservation technology and discloses a method for preserving mangoes containing chitosan, a eutectic solvent, and EGC. The method includes the following steps: adding chitosan to an acetic acid solution and heating and stirring in a water bath to obtain a chitosan film solution; pre-dispersing and wetting epigallocatechin in a eutectic solvent, then adding it to the aforementioned chitosan film solution and stirring at room temperature to obtain a modified chitosan composite film solution; selecting undamaged mangoes, washing and air-drying their surface moisture, immersing them in the modified chitosan composite film solution to evenly coat the surface with the film solution, removing them and air-drying them naturally, repeating the aforementioned immersion and air-drying process, and finally storing the dried mangoes in an indoor environment for preservation. This invention utilizes a eutectic solvent to solve the problem of easy aggregation of polyphenolic active substances, forming a uniform and continuous semi-permeable membrane on the mango peel, limiting moisture loss, achieving slow release of antioxidant components, and significantly extending the mango's shelf life.

The present disclosure relates essentially to a supplement, the function of which is to enhance immunity, prevent various diseases and assist in therapeutic treatments of various diseases, said supplement includes: 100 mg of lipoic acid, 1 mg of astaxanthin, 10 mg of glutathione, 5 mg of resveratrol, 5 mg of lycopene, 50 mg of epigallocatechin, 300 mg of curcumin, 400 mg of quercetin, 10 mg of coenzyme Q1 and 100 mg of dry açaí extract; formulated with the intention of combining substances that serve functions of assisting in the treatment of various diseases, such as: lipoic acid, which helps in the prevention of liver damage from intoxication, and can be used as a coadjuvant for the treatment of liver cirrhosis due to alcoholism and a coadjuvant for the treatment of diabetes and cataracts.

The disclosure relates to crystalline Form X of epigallocatetchin-3-gallate, compositions containing Form X, and methods of using Form X. The disclosure also relates to methods of making crystalline Form X of epigallocatetchin-3-gallate.

To provide a heat-sterilized milk-containing beverage with reduced deterioration smell.SOLUTION: In a heat-sterilized milk-containing beverage, epigallocatechin and an inorganic salt including magnesium salt are formulated. The epigallocatechin content in the beverage is 0.1 to 20.0 mg / 100 ml. The magnesium content in the beverage is 0.1 to 20.0 mg / 100 ml.SELECTED DRAWING: None

The invention discloses an aleurites montana anthocyanin reductase VmANR gene and application thereof. The amino acid sequence of the aleurites montana anthocyanin reductase VmANR gene is shown as SEQ ID NO. 2. The content of compounds such as catechin, (-)-epicatechin, (-)-epigallocatechin, phlorizin and dihydromyricetin in transgenic hairy root plants over-expressed by the VmANR obtained by a transgenic means is remarkably increased, so that more flavonoid compounds can be extracted, separated and purified, and greater economic benefits are created; the method provides an important theoretical basis for increasing the content of flavonoid compounds in plants by using a biotechnological means in the future, and has a relatively high application value.

The invention relates to self-degradable environment-friendly pet deodorization particles as well as a preparation method and application thereof, and relates to the technical field of pet deodorization. The deodorization particles for the pets are prepared from the following raw materials in parts by weight: 3 to 10 parts of chitosan-starch based modified materials, 1 to 3.5 parts of yucca plant extracts, 0.3 to 1.2 parts of gallocatechin, 0.45 to 2.4 parts of ellagic acid, 0.02 to 0.08 part of imine type COFs (Covalent Organic Frameworks) TpPa-1 and 0.02 to 0.05 part of gamma-terpineol. The self-degradable environment-friendly pet deodorization particle has good degradability and excellent deodorization and bacteriostasis effects, and the performance is improved through the synergistic effect of all the raw materials.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly provides a construction method and a content determination method of specific chromatograms of a Guangxi kadsura pepper stem aqueous extract and a preparation thereof, and the method comprises the following steps: (1) preparing a reference substance solution; (2) preparing a test solution; (3) detecting by adopting an ultra-high performance liquid chromatography to obtain seven or more common characteristic peaks, and realizing effective separation of the characteristic peaks containing gallic acid, protocatechuic acid, gallocatechin, protocatechualdehyde, catechin and epicatechin, so that the peak shape is good, and the base line is stable; and (4) taking catechin as an internal reference substance, establishing relative correction factors of protocatechuic acid, epicatechin and catechin, and establishing a content determination method of quantitative analysis of multi-components by single marker of three components. According to the method constructed by the invention, the content of the characteristic chromatogram and the content of the characteristic components can be simultaneously detected, the measured components can be completely separated, the detection time is short, and a basis is provided for quality detection and control of the Guangxi kadsura pepper stem aqueous extract and preparations thereof.

The invention provides an application of epigallocatechin-3-gallate in preparation of an acute respiratory distress syndrome product. The epigallocatechin-3-gallate can be used for treating acute respiratory distress syndrome. According to the application disclosed by the invention, the ARDS induced by lipopolysaccharide is treated by utilizing the epigallocatechin-3-gallate and improving intestinal microorganisms and metabolite short-chain fatty acid, and a new way is opened up for the treatment of the ARDS.

There has been need for tea beverages that maintain characteristics thereof while maintaining drinkability even with a low pH. One aspect of the present invention provides a beverage containing diacetyl and catechins, wherein the diacetyl content is at least 20 ppb and less than 10,000 ppb, the contained amount one or more types of catechins selected from epicatechin, catechin, epigallocatechin, gallocatechin, epicatechin gallate, catechin gallate, epigallocatechin gallate, and gallocatechin gallate is at least 1 ppm and less than 500 ppm, and the pH is 5 or lower.

The invention discloses a preparation method and application of a plant extract fermentation product for promoting anti-aging of cats, and belongs to the technical field of animal health care product preparation. Comprising a metabolite (LA20FM) of a brown algae extract which has an anti-aging function and is fermented by bifidobacterium adolescentis LifeAge20 and other raw materials, including one or more of D-sphingosine, luteolin, epigallocatechin, syringic acid, leucine, fucosterol, resveratrol, nicotinamide and glutathione. The metabolite of the brown algae extract fermented by the bifidobacterium adolescentis LifeAge20 disclosed by the invention has a remarkable effect in the anti-aging aspect, and can improve the content of nicotinamide adenine dinucleotide in vascular endothelial cells of cats, the angiogenesis capability and the antioxidant capability; the activity of beta-galactosidase in cells and the content of senescence phenotype markers are reduced, and a multi-channel anti-aging effect is realized. The LA20FM and additives are added to prepare the health-care agent, so that the health-care agent is more beneficial to anti-aging of pets and has a wide application prospect.

The invention discloses a method for selectively producing EGCG-4 '-O-alpha-D glucopyranoside by using sucrose phosphorylase, and belongs to the technical field of biological catalytic synthesis. An engineered mutant derived from leuconostoc mesenteroides sucrose phosphorylase (LmSPase) is used as a catalyst. The mutant is constructed through single-point or combined mutation on the basis of a wild type with the sequence of SEQ ID NO.1, and the enzyme activity, the stability and the substrate specificity of the mutant are all remarkably improved. By utilizing the high-performance mutant, a method for efficiently synthesizing (-)-epigallocatechin gallate 4 '-O-alpha-D glucopyranoside is established. The method is mild in reaction condition, simple and convenient to operate, short in reaction time and high in catalytic efficiency, and shows good application potential.

The present invention discloses a preparation method and application of an indicator displacement sensor array for tea polyphenol identification. The array is prepared by using two arylboronic acids, 1-naphthaleneboronic acid (NapB) and 1-pyreneboronic acid (PyrB), as probes, and catechol-containing Alizarin Red S (ARS) and catechol violet (PV) as indicators. Based on the indicator displacement method (IDA) mechanism, a 2×2 colorimetric sensor array is designed and constructed for detecting and identifying different types of tea polyphenols, namely gallic acid, methyl gallate, epicatechin, epigallocatechin, epigallocatechin gallate, and epicatechin gallate. The binding force between tea polyphenols and the boronic acid probe is stronger than that of the indicator. Different tea polyphenols have different binding abilities to the probe and different abilities to displace the indicator, resulting in different colorimetric responses of the IDA sensor array to tea polyphenols. In addition, the colorimetric sensing array was combined with principal component analysis (PCA) to successfully distinguish different types of tea polyphenols.

The application discloses a tea characteristic component composition for reducing acrylamide in Maillard reaction, and the composition is prepared by compounding at least three of five substances, i.e., epicatechin (EC), gallocatechin (GC), catechin gallate (CG), theabrine and theaflavine, and the compounded composition has the effect of reducing acrylamide, a by-product of Maillard reaction in food. In the composition of the application, the composition composed of epicatechin (EC), gallocatechin (GC), catechin gallate (CG) and theabrine has the best effect of reducing acrylamide, a by-product of Maillard reaction, and the reduction rate of acrylamide in a simulation system can be up to 75%. The composition of the application can be applied to baked food or fried food, especially baked food such as bread, cookie, potato chip and the like.

The invention discloses a macromolecular antioxidant as well as a preparation method and application thereof, and belongs to the technical field of plant active ingredient extraction. According to the method, kiwi fruit peel is used as a raw material, a pectin macromolecular antioxidant rich in combined polyphenol is extracted through a dilute alkali heating method, the molecular weight of the obtained extract is 56.51 kDa, 17 phenolic acid compounds such as gallic acid, ferulic acid, caffeic acid and salicylic acid are identified, and the content of the phenolic acid compounds is more than or equal to 1%. 18 flavonoid compounds such as catechin, epigallocatechin and quercitrin, 4 coumarin compounds and 3 other phenolic substances have high DPPH free radical scavenging capacity, ABTS free radical scavenging capacity and Fe < 3 + > reducing capacity, and high-value utilization of waste plant resources in the kiwi fruit processing industry is achieved.

This invention provides a method for synthesizing novel natural pigments using phenotypic catechins. Various epicatechins, epigallocatechins, epicatechin gallate esters, and epigallocatechin gallate esters are used as substrates. These are mixed and subjected to a non-enzymatic oxidation reaction under humid and hot conditions to generate a new class of tea pigment compounds. The non-enzymatic oxidation products of the phenotypic catechins are then concentrated under reduced pressure, freeze-dried, extracted, and purified by column chromatography to obtain dimeric catechin pigments. The six novel dimeric catechin pigments obtained by this invention are synthesized using a simple method. The six novel dimeric catechin pigments obtained according to the method of this invention are orange-yellow or orange-red amorphous powders with good color-adjusting capabilities, and can be applied to coloring tea beverages and new-style tea drinks. The discovery of this type of natural pigment contributes to the research on coloring substances during the thermal processing of tea, the improvement of tea liquor color quality, and the development and application in the field of food coloring.

The invention discloses a sucrose phosphorylase mutant and application of the sucrose phosphorylase mutant in synthesis of epigallocatechin gallate glucoside, and belongs to the technical field of enzyme engineering and biological catalysis. The sucrose phosphorylase mutant is obtained by mutating a plurality of amino acid sites on the basis of an amino acid sequence of SEQ ID NO.1. The mutant not only remarkably improves the enzyme activity, but also more importantly changes the regioselectivity of the mutant; by utilizing the mutant, efficient catalytic synthesis of (-)-epigallocatechin gallate 4 ''-O-alpha-D glucopyranoside is realized, and a key foundation is laid for stable industrial production and large-scale application.