6 results about "Cefalexin" patented technology

This invention discloses a rapid detection method for cephalexin and lead using a diatomic nanozyme. The method involves synthesizing Fe and Cu coordinated teratophorphene (4-carboxyphenyl)-1, which is then pyrolyzed at high temperature to form a diatomic nanozyme (Cu,Fe-N-C). The coexistence of iron and copper active sites in Cu,Fe-N-C exhibits high peroxidase-mimicking enzyme activity due to its synergistic effect. Cephalexin significantly inhibits the Cu,Fe-N-C + TMB + H₂O₂ system. The complexation of cephalexin with metallic Cu reduces the affinity of Cu,Fe-N-C for the substrate and the number of active sites, leading to a decrease in the absorbance of the system. Meanwhile, Pb... 2+ The addition of Pb restored the activity of the peroxidase nanozyme. 2+ Competitively forming a complex with cephalexin, it releases the iron and copper active sites in the Cu,Fe-N-C nanozyme, leading to increased affinity of Cu,Fe-N-C for the substrate and a corresponding increase in absorbance. This establishes a novel, highly sensitive, and selective rapid detection method for cephalexin and lead, with limits of quantitation of 0.010 and 0.012 mg / kg, respectively, meeting relevant national food safety requirements. This method is characterized by its simplicity, high sensitivity, and rapid operation.

The invention discloses an archaia adheris DLW198 and application of the archaia adheris DLW198. Belongs to the technical field of antibiotic degradation. According to the present invention, the cefoperazone concentration tolerable by the concorchus chinensis DLW198 is 50-250 mg / L, the inoculation amount of the concorchus chinensis is 5-25%, the degradation temperature is 20-40 DEG C, the pH value is 5-9, and the concorchus chinensis can effectively degrade the cefoperazone under the conditions of the concorchus chinensis DLW198, under the conditions that the temperature is 30 DEG C, the pH value is 7, the inoculum size is 20% and the rotating speed is 200 r / min, the degradation rate of the strain DLW198 to 200 mg / L cefoperazone reaches 92.98% within 16 h. In addition, the strain DLW198 also can be used for degrading cefalexin, ceftriaxone sodium, cefixime and amoxicillin. The strain is relatively high in biological safety, does not harm human health or pollute the environment in the application process, and has important application value.

The invention provides a preparation method of L-phenylglycine methyl ester hydrochloride, and belongs to the technical field of pharmaceutical chemicals. The preparation method comprises the following steps: deoxidizing a reactor, adding methanol and L-phenylglycine, adding triphosgene in batches at the temperature of-5 to 10 DEG C, and heating to 60 to 70 DEG C for esterification reaction after the triphosgene is completely fed; when it is detected that the purity content of the L-phenylglycine methyl ester hydrochloride is 95% or above, the obtained L-phenylglycine methyl ester hydrochloride mother liquor is concentrated under reduced pressure to be dry; and adding pure water for dissolving, adjusting the pH value to 1.0-3.0, adjusting the temperature to below 20 DEG C, decolorizing, degassing and filtering to obtain an aqueous solution of the L-phenylglycine methyl ester hydrochloride. Compared with a thionyl chloride method for preparation, the method has remarkable advantages in the aspects of production storage, waste gas treatment and the like, the production cycle can be greatly shortened compared with a sulfuric acid method, and the method can be directly used for side chain enzymatic synthesis of beta-lactam antibiotics such as ampicillin, cefalexin and cefaclor.

The invention belongs to the technical field of biosensing, and relates to a preparation method of a cefalexin rapid detection chip for detecting antibiotics in food. Polypyrrole-chitosan quaternary ammonium salt (PANI-QCS) hydrogel with excellent conductivity and biocompatibility is prepared through emulsion polymerization and coupling reaction, and is fixed on a silk-screen printing carbon electrode to construct a cefalexin electrochemical sensing chip with high selectivity and quick response. The sensing microchip has excellent conductivity and biocompatibility, and due to the special structure, the sensing microchip has a high specific surface area. By fixing the cefalexin antibody, the biosensing microchip can realize rapid detection of cefalexin in milk within 20 minutes so as to help consumers to identify safe products and help supervision organizations to carry out large-scale and high-frequency screening, so that food compliance is ensured, and drug resistance diffusion is suppressed.

The invention relates to a hybridoma cell strain secreting a ceftazidime monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. A hybridoma cell strain capable of accurately detecting low-concentration ceftazidime is separated, specifically, a monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to the ceftazidime, the IC50 value of the monoclonal antibody to the ceftazidime is 0.40 ng / mL, the IC50 value of the monoclonal antibody to the ceftazidime is 0.40 ng / mL, and the IC50 value of the monoclonal antibody to a structural analogue of the ceftazidime is 0.40 ng / mL. According to the present invention, ceftazidime with low concentration can be accurately detected, such as cefazolin, cefradine, cefalexin, cefquinoline and cefalotin, no cross reaction exists, and the specificity is good, such that the low-concentration ceftazidime can be accurately detected.

The application discloses a kind of adhering arrow bacteria DLW198 and application.It belongs to the technical field of antibiotic degradation.The adhering arrow bacteria DLW198 of the application can tolerate the concentration of cefoperazone is 50-250mg / L, the inoculation amount of adhering arrow bacteria is 5%-25%, the degradation temperature is 20-40 DEG C, and pH is 5-9, under the above conditions, adhering arrow bacteria can effectively degrade cefoperazone.Under the conditions of temperature 30 DEG C, pH 7, inoculation amount 20%, and rotation speed 200r / min, the degradation rate of strain DLW198 to 200mg / L cefoperazone reaches 92.98% in 16h.In addition, strain DLW198 can also degrade cephalexin, ceftriaxone sodium, cefixime and amoxicillin.The strain has higher biological safety, and will not endanger human health or pollute the environment in the application process, and has important application value.