3 results about "Cefalexin" patented technology

This invention discloses a rapid detection method for cephalexin and lead using a diatomic nanozyme. The method involves synthesizing Fe and Cu coordinated teratophorphene (4-carboxyphenyl)-1, which is then pyrolyzed at high temperature to form a diatomic nanozyme (Cu,Fe-N-C). The coexistence of iron and copper active sites in Cu,Fe-N-C exhibits high peroxidase-mimicking enzyme activity due to its synergistic effect. Cephalexin significantly inhibits the Cu,Fe-N-C + TMB + H₂O₂ system. The complexation of cephalexin with metallic Cu reduces the affinity of Cu,Fe-N-C for the substrate and the number of active sites, leading to a decrease in the absorbance of the system. Meanwhile, Pb... 2+ The addition of Pb restored the activity of the peroxidase nanozyme. 2+ Competitively forming a complex with cephalexin, it releases the iron and copper active sites in the Cu,Fe-N-C nanozyme, leading to increased affinity of Cu,Fe-N-C for the substrate and a corresponding increase in absorbance. This establishes a novel, highly sensitive, and selective rapid detection method for cephalexin and lead, with limits of quantitation of 0.010 and 0.012 mg / kg, respectively, meeting relevant national food safety requirements. This method is characterized by its simplicity, high sensitivity, and rapid operation.

The invention relates to a hybridoma cell strain secreting a ceftazidime monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. A hybridoma cell strain capable of accurately detecting low-concentration ceftazidime is separated, specifically, a monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to the ceftazidime, the IC50 value of the monoclonal antibody to the ceftazidime is 0.40 ng / mL, the IC50 value of the monoclonal antibody to the ceftazidime is 0.40 ng / mL, and the IC50 value of the monoclonal antibody to a structural analogue of the ceftazidime is 0.40 ng / mL. According to the present invention, ceftazidime with low concentration can be accurately detected, such as cefazolin, cefradine, cefalexin, cefquinoline and cefalotin, no cross reaction exists, and the specificity is good, such that the low-concentration ceftazidime can be accurately detected.

The application discloses a kind of adhering arrow bacteria DLW198 and application.It belongs to the technical field of antibiotic degradation.The adhering arrow bacteria DLW198 of the application can tolerate the concentration of cefoperazone is 50-250mg / L, the inoculation amount of adhering arrow bacteria is 5%-25%, the degradation temperature is 20-40 DEG C, and pH is 5-9, under the above conditions, adhering arrow bacteria can effectively degrade cefoperazone.Under the conditions of temperature 30 DEG C, pH 7, inoculation amount 20%, and rotation speed 200r / min, the degradation rate of strain DLW198 to 200mg / L cefoperazone reaches 92.98% in 16h.In addition, strain DLW198 can also degrade cephalexin, ceftriaxone sodium, cefixime and amoxicillin.The strain has higher biological safety, and will not endanger human health or pollute the environment in the application process, and has important application value.