49 results about "Carnosic acid" patented technology

The invention relates to the technical field of plastic packaging preparation, in particular to an anti-aging plastic packaging material and a preparation method thereof.The anti-aging plastic packaging material is prepared from, by mass, 100-140 parts of polylactic acid, 60-80 parts of PBAT, 2-5 parts of a composite anti-aging agent, 1-2 parts of a lubricant, 2-4 parts of a flame retardant and 3-6 parts of a compatilizer; the composite anti-aging agent comprises a modified anti-aging agent obtained by compounding functionalized montmorillonite and modified silicon dioxide, the functionalized montmorillonite is carnosic acid grafted rare earth ion modified montmorillonite, and the modified silicon dioxide is epoxy methyl oleate grafted silicon dioxide. According to the anti-aging plastic packaging material provided by the invention, a plastic material system with multiple protection mechanisms is constructed through the synergistic effect of all the components, and the prepared plastic packaging material not only keeps good biodegradability, but also has excellent ultraviolet light resistance and oxidation resistance.

The invention relates to a natural antioxidant for prefabricated vegetables and a preparation method of the natural antioxidant, and belongs to the technical field of food additives. The antioxidant is prepared from rosmarinic acid, carnosic acid, drug-loaded cellulose and double-phase chelating microspheres; the drug-loaded cellulose is prepared by modifying cellulose with quercetin, loading anthocyanin and then wrapping with a trehalose-gamma-polyglutamic acid composite film; the biphase chelating microspheres are phytic acid-citric acid biphase sustained-release microspheres formed by wrapping phytic acid and citric acid with a chitosan and sodium alginate composite film. Through multi-component functional complementation, multi-stage slow release design and physical-chemical synergistic barrier, comprehensive inhibition of the oxidation problem of the prefabricated vegetable is realized, a'free radical scavenging-metal chelating-phase interface stabilization 'triple protection system is constructed, accurate oxidation resistance in different stages of processing, storage and transportation is realized, and the prepared vegetable has good market prospects. The bottlenecks of short action period and single protection dimension of a natural antioxidant are broken through.

The present invention discloses an anti-aging composition, an anti-aging cosmetic and its preparation method and application. The anti-aging composition includes torreya grandis oil, hydrolyzed Brazil nut protein, angoloside C, carnosic acid and nanovesicle excipients. The present invention uses the above four natural plant components as anti-aging active ingredients, and from multiple perspectives such as promoting collagen synthesis, inhibiting the expression of inflammatory factors, scavenging free radicals, and repairing DNA damage, it exerts a significant anti-aging effect. The present invention encapsulates the above anti-aging active ingredients in the form of nanovesicles, which not only improves the stability of the active ingredients, but also improves the skin absorption effect of the active ingredients. The liposome nanovesicles have good compatibility with the matrix of the cosmetic, effectively solving the compatibility problem between the active ingredients and the cosmetic matrix.

The invention belongs to the technical field of lubricating oil manufacturing, and relates to composite lubricating oil used in an aluminum rolling process, which comprises the following components in parts by mass: 94-96 parts of base oil, 0.5-1.5 parts of an oiliness agent, 0.5-0.8 part of an extreme pressure anti-wear agent, 0.5-1.5 parts of a wear-resistant antioxidant, 0.5-2 parts of oleic acid diethanolamide and 0.5-1 part of an antirust agent. On the basis of the prior art, nano-sheet molybdenum disulfide is introduced and added into 2-aminoterephthalic acid and nickel nitrate to react to prepare a modified metal organic framework, then carnosic acid is grafted through an amide reaction to prepare the wear-resistant antioxidant, and a protective film can be formed on a friction interface through a sheet structure of molybdenum disulfide, so that the friction coefficient is reduced; the wear resistance is improved; a compact passive film is formed on the surface of aluminum metal through the synergistic effect of the extreme pressure anti-wear agent and the wear-resistant antioxidant, and the corrosion resistance and wear resistance of the composite lubricating oil used in aluminum plate rolling are improved.

The invention provides high-naringenin-content modified shaddock peel fiber gel and a preparation method thereof.The preparation method comprises the following steps that after shaddock peel white pulp is subjected to quick freezing and smashing, pretreatment is conducted through an electrochemistry-assisted gradient enzymolysis technology, then customized immobilized naringinase is adopted for treatment, and high-naringenin-content shaddock peel fibers are obtained; and finally, uniformly mixing the fiber with carnosic acid and soybean saponin, and adding magnesium chloride for cross-linking and curing to obtain the modified shaddock peel fiber gel. According to the preparation method, electrochemical assisted gradient enzymolysis is adopted, so that a compact network structure of fibers can be destroyed to increase active sites, the immobilized naringinase not only can efficiently convert naringin and accurately enrich naringenin, but also can effectively improve bitter taste, and magnesium ions regulate and control a multiple cross-linked network formed by the components, so that the gel structure tends to be more stable; meanwhile, naringenin is stably loaded so as to avoid migration and aggregation of the naringenin, and the bioavailability and active efficacy of the naringenin are remarkably improved.

The invention discloses application of carnosic acid as an agricultural bactericide and a synergist in plant diseases and a composition of the carnosic acid, and the carnosic acid has a remarkable inhibition effect on colletotrichum sorghum and is superior to a positive control drug azoxystrobin. In addition, carnosic acid is used as a synergist and is compounded with a QoI bactericide capable of inhibiting mitochondrial respiration and / or an SDHI succinate dehydrogenase inhibitor, so that the bactericidal activity can be remarkably improved. The antibacterial effect of the bactericidal composition is greatly improved by compounding the bactericidal composition with azoxystrobin, and the anti-colletotrichum sorghum result shows that when the compounding ratio of azoxystrobin to carnosic acid is 1: 4, the synergistic coefficient SR value is 1.637, and the bactericidal composition has a remarkable synergistic effect.

The present invention belongs to the technical field of biological medicine, and discloses a ceramide compound shown in Chemical Formula I: #imgabs0# wherein, R 1 is selected from the residues after condensation of sialic acid, chelidamic acid monohydrate, 2,5-furandicarboxylic acid, quinic acid, chlorogenic acid, ectoine, nicotinic acid, shikimic acid, glucuronic acid, salicylic acid, carnosic acid, dihydrooat acylanthranilic acid, abietic acid, ursolic acid, tranexamic acid, syringic acid, gentisic acid, vanillic acid, 5-hydroxyisovanillic acid, orotic acid, 5-aminoorotic acid, 3,5-dihydroxy-4-methylbenzoic acid, glycyrrhizic acid or gallic acid, R 2 is selected from one of the following structures: -C 15 H 29 、-C 15 H 31 、-C 15 H 27 、-CHOHC 14 H 27 、-CHOHC 14 H 29 . The present invention also discloses a preparation method and application of the ceramide compound. The present invention introduces a molecular fragment with physiological activity of a functional carboxylic acid into ceramide, which will enhance the original efficacy of this type of molecule and also improve the physicochemical properties of the ceramide compound.

There is provided a composition comprising carnosic acid and tocopherols as acrylamide reducing agent, as well as methods and uses of such composition and products obtained when using such composition.

A high-solubility pet nutritional composition, characterized in that it comprises an oil-soluble component, an emulsifier, a thickener, and an acidity regulator, wherein the oil-soluble component is selected from EPA, DHA, lauric acid, carnosic acid, linoleic acid, or one or more thereof; the emulsifier is selected from casein, whey protein, phospholipids, polysaccharides, octenylsuccinic acid, sodium starch, or one or more thereof; the thickener is selected from cellulose, locust bean gum, xanthan gum, sodium alginate, sodium carboxymethylcellulose, chitosan, or one or more thereof; the acidity regulator is selected from citric acid, sodium citrate, potassium citrate, malic acid, or lactic acid, or one or more thereof. the weight ratio of the oil-soluble component, the emulsifier, the thickener and the acidity regulator is (20-50) : (1-20) : (0.1-10) : (0.1-10).

The invention discloses an application of ARN2966 in culture of renal cells. The kidney-derived cells comprise renal tubular epithelial cells and urine-derived cells from kidneys. According to the present invention, it is found that the Corydaline, the ARN2966, the PKG drg G1, the Succinobcol, the Meptyldinocap, the Rifamycin sodium salt, the Carnosic acid, the TBHQ, the C188-9 and the Buparvaquone can maintain the long-term culture of the primary human renal tubular epithelial cells, and the cell activity can be improved; the application also finds that the Corydaline, the ARN2966 and the PKG drg G1 have an obvious cell viability improving effect and even a proliferation promoting effect on the urine-derived cells from the kidney, and can ensure that more urine-derived cells can be obtained at an early generation.

The invention discloses a method for improving antioxidant capacity of a rosemary extract by regulating and controlling synthesis of carnosic acid, and belongs to the field of molecular biology. According to the invention, the SrMYB177 gene positioned in a cell nucleus is found, and overexpression of the SrMYB177 gene in rosemary significantly increases the carnosic acid content and increases the antioxidant ability of rosemary; through separation of SrMYB177 and identification and analysis of gene functions, it is determined that SrMYB177 positively regulates and controls synthesis of carnosic acid in rosemary so as to increase the oxidation resistance of plants, the SrMYB177 gene can precisely regulate and control synthesis of carnosic acid in plants and increase the content of carnosic acid, the SrMYB177 gene can be used for cultivating transgenic plants, plants with the high carnosic acid content are obtained, and the SrMYB177 gene can be applied to the field of cultivation of plants with the high carnosic acid content. Wide application prospects are realized in the fields of foods, medicines, cosmetics and the like.

The invention relates to a method for extracting water-soluble components from rosemary, a method for preparing rosemary emulsion, the rosemary emulsion and application, and belongs to the technical field of rosemary extraction. The method for extracting the water-soluble components comprises the following steps: sequentially extracting rosemary essential oil and carnosic acid in rosemary dry leaves by adopting a supercritical CO2 fluid extraction technology to obtain rosemary dry leaves I; the method comprises the following steps: sealing and soaking rosemary dry leaves I in 30-60wt% ethanol for 1-3 hours, then putting the rosemary dry leaves I into an extraction kettle, introducing CO2, keeping the pressure at 20-35MPa, the extraction temperature at 30-60 DEG C and the extraction time at 4-8 hours, and collecting extract liquor; the mass ratio of the rosemary dry leaves I to the ethanol is 1: (2-15); and carrying out solid-liquid separation on the extract, and drying to obtain the water-soluble rosemary extract. The activity of the water-soluble component extracted by the method is higher, and meanwhile, the rosemary emulsion with a finer and more stable wrapping effect is obtained by matching with the method for preparing the rosemary emulsion.

The invention relates to the technical field of traditional Chinese medicines, in particular to a microcapsule for promoting sleep and a preparation method thereof. The hevea brasiliensis seed oil, the panax notoginseng extract, the semen cassiae extract, the lotus leaf extract and the gastrodia elata extract are reasonably combined for the first time, the effects are mutually matched, the gastrointestinal tract can be effectively regulated, absorption and biological utilization of active substances are promoted, and the effect of improving sleep for a long time is achieved; a natural raw material double-layer microcapsule embedding process is adopted, carnosic acid is selected to be introduced into a capsule core system, the stability of the capsule core system is improved, inulin is selected to be introduced into a capsule material system, intestinal tract movement is promoted, meanwhile, the carnosic acid is preferentially utilized by probiotics such as bifidobacterium and lactobacillus, and the actual effect and bioavailability of the microcapsule are improved to a large extent; according to the preparation method, the vitamin E membrane is formed outside the condensation nucleus, so that the performance of the composite wall material is integrally improved, and the prepared microcapsule has strong oxidation resistance, so that the microcapsule can endure the test of gastric acid to be released and proliferated at a fixed point in the intestinal tract, and the bioavailability is improved.

The application relates to the field of biotechnology, and particularly relates to the application of carnosic acid as an ERAP1 inhibitor in the preparation of a drug for treating ankylosing spondylitis of an autoimmune disease. The application first discovers that a monomer compound carnosic acid from a natural product source can be used as an ERAP1 direct targeting inhibitor in the preparation of a drug for regulating an endogenous antigen processing and presentation pathway and ankylosing spondylitis of a related autoimmune disease. The carnosic acid can be specifically combined in an ERAP1 active site through direct high-activity targeting, and the carnosic acid inhibits the activity of the ERAP1, is the highest in activity in the world, and has high selectivity in targeting the ERAP1 active site. The carnosic acid reverses the ankylosing spondylitis molecular pathological phenotypes caused by abnormal ERAP1 activity, including endoplasmic reticulum stress, reducing an extra amount of HLA-B27 molecules displayed on a cell surface, reducing the proportion of the HLA-B27 molecules incorrectly assembled on the cell surface, and maintaining normal antigen presentation functions.

The invention discloses a method for synthesizing carnosic acid in vitro and application of the carnosic acid in improvement of oxidation resistance, and belongs to the field of molecular biology. According to the application disclosed by the invention, SrCYP716A514 and SrCYP76AK7 genes positioned in endoplasmic reticulum are found, the SrCYP716A514 and SrCYP76AK7 genes are found to be capable of synthesizing the carnosic acid in vitro, and the content of the carnosic acid is obviously increased when the SrCYP716A514 and SrCYP76AK7 genes are overexpressed in rosemary, and the oxidation resistance of a rosemary extract can be enhanced. Through separation and functional identification analysis of the SrCYP716A514 gene and the SrCYP76AK7 gene, it is determined that the SrCYP716A514 gene and the SrCYP76AK7 gene can synthesize carnosic acid in vitro, the content of the carnosic acid is remarkably increased through overexpression in rosemary, and the oxidation resistance of a rosemary extract can be enhanced. According to the invention, synthesis of carnosic acid in plants can be accurately regulated and controlled, the carnosic acid content can be increased, the method can be used for cultivating transgenic plants, plants with high carnosic acid content can be obtained, and the method has a wide application prospect in the fields of food, medicines, cosmetics and the like.

The breeding method for the postpartum blue crabs is characterized by comprising a microcirculation preparation for the postpartum blue crabs, and the microcirculation preparation for the postpartum blue crabs at least contains one of the following components in content: rosmarinic acid with the content of 300-400 mg / L, carnosic acid with the content of 250-350 mg / L, oleuropein with the content of 250-350 mg / L and terpene alcohol with the content of 200-300 mg / L. The microcirculation preparation for the postpartum blue crabs can remarkably improve the growth level, the nutrition transportation capacity, the waste metabolism capacity, the tissue repair capacity and the fat and energy storage capacity of the postpartum female crabs, and promote the postpartum cream increase and rejuvenation of the blue crabs.

Stabilized oils including an edible oil and an antioxidant composition comprising α-lipoic acid and least one of ascorbic acid, ascorbyl palmitate, green tea extract, lecithin, and rosemary extract or at least one of 1,2,4-benzenetriol, carnosic acid, dihydromyricetin, dihydrorobinetin, epigallocatechin, gallic acid, 3-hydroxytyrosol, myricetin, and nepodin, and methods of preparing such stabilized edible oils. The stabilized oils may have an Oxidative Stability Index (“OSI”) at 110° C. of at least 30 hours.

The invention discloses a pesticide preparation containing fluopyram and abamectin as well as a preparation method and application of the pesticide preparation, and belongs to the technical field of pesticide preparations. The active ingredients of the pesticide preparation provided by the invention comprise fluopyram, abamectin, caryophyllene and caryophyllene, the pesticide preparation is prepared by adopting a complex coacervation technology of'acid first and alkali second ', and the pesticide preparation has pH and enzyme dual stimulation response characteristics, and can realize targeted release of infected parts of diseases and insect pests. According to the invention, plant source active substances including caryophyllene and caryophyllene and efficient chemical pesticides including fluopyram and abamectin are scientifically matched, so that synergistic interaction is realized, and the co-toxicity coefficient of the prepared pesticide preparation is greater than 130; the obtained product is good in stability and long in lasting period, the dosage of chemical pesticides can be effectively reduced, and the risk of environmental pollution is reduced.

The present invention relates to the field of plastic packaging preparation technology, and in particular to a kind of anti-aging plastic packaging material and preparation method thereof, comprising the following components by mass: 100-140 parts of polylactic acid, 60-80 parts of PBAT, 2-5 parts of composite anti-aging agent, 1-2 parts of lubricant, 2-4 parts of flame retardant and 3-6 parts of compatibilizer, wherein the composite anti-aging agent includes a modified anti-aging agent obtained by compounding functionalized montmorillonite and modified silica, wherein the functionalized montmorillonite is carnosic acid grafted rare earth ion modified montmorillonite, and the modified silica is epoxy methyl oleate grafted silica. The anti-aging plastic packaging material provided by the present invention constructs a plastic material system with multiple protection mechanisms through the synergistic effect of each component, and the prepared plastic packaging material not only maintains good biodegradability, but also has excellent anti-ultraviolet light and antioxidant properties.

The present invention relates to a standardized polyphenol substance formulation, which has an anti-cancer effect; due to oxidation resistance, inflammation resistance, liver protection, heart protection, nerve protection and systemic effects, cytotoxicity can be selectively generated on tumor cells, so that the effectiveness of standard treatment is supported; and when the medicine is used according to the recommended dosage, compared with medicines with the characteristics of cancer resistance, oxidation resistance, inflammation resistance and the like in the prior art, the medicine has the minimum side effect. The formulation mentioned consists of a combination comprising at least one of the following natural substances: a plant derived curcumin, oleuropein, epigallocatechin gallate (EGCG), resveratrol, rosmarinic acid, carnosic acid and phenethyl caffeate (CAPE), apigenin, galangin, chrysin, luteolin, quercetin and pinocembrin, and a combination comprising at least one of the following natural substances: a plant derived curcumin, oleuropein, epigallocatechin gallate (EGCG), resveratrol, rosmarinic acid, carnosic acid and phenethyl caffeate (CAPE), the natural substances exist in various plants and propolis. The active polyphenol substance content in the formulations as the subject matter of the invention varies depending on the type and stage of cancer the formulations will be used to treat. Formulations as the subject matter of the invention are prepared in the form of suspensions, syrups, pouches, tablets or capsules, containing in addition to the active ingredients an appropriate proportion of excipients such as dextrose, maltose, maltodextrin or beta-cyclodextrin.

The invention relates to the technical field of cosmetic anticorrosion, and particularly discloses a mild anticorrosion composition and a preparation method and application thereof.The composition is prepared from, by mass, 13.0%-16.7% of oil-soluble alcohol, 2.0%-5.0% of fatty acid salt, 1.0%-3.0% of plant extract, 1%-3% of polyol ester and the balance antibacterial grease; the fatty acid salt is zinc octoate and sodium lauroyl sarcosinate; the plant extract is a moutan bark extract and carnosic acid; the components take effect synergistically to achieve long-acting antibacterial and antiseptic effects, the components are mild and non-irritant, synergistic bacteriostasis is achieved through multiple mechanisms of pH adjustment, microbial cell membrane damage, metabolic enzyme activity inhibition and the like, the oil solubility is excellent, and the composition is suitable for pure oil agents, water-in-oil type cream and oil-in-water type cream. The problems that a traditional preservative is strong in irritation, poor in oil phase dispersion and difficult to pass the international standard test are solved, and the preservative has the characteristics of mildness, high efficiency and low addition amount.

The invention relates to the field of emulsifying agents for food, provides an instant meat product emulsifying agent and a preparation method thereof, and solves the defect that the emulsifying effect of an emulsifying agent in the prior art is relatively common. Comprising the following raw materials in parts by weight: 30-40 parts of modified chickpea protein isolate, 20-30 parts of enzymolysis modified pea protein, 20-30 parts of inulin, 7-10 parts of kappa-carrageenan, 3-5 parts of konjac glucomannan, 4-6 parts of sunflower lecithin, 3-8 parts of glycerol monolaurate, 2-4 parts of trehalose and 0.4-0.6 part of a rosemary extract. The hydrolysis degree of the enzymolysis modified pea protein is 4-8%, the average polymerization degree of the inulin is greater than or equal to 10, and the content of carnosic acid in the rosemary extract is greater than or equal to 22%.

A composition comprising natural pyrethrin, one or more phenolic antioxidants selected from Group A, and a solid carrier;　wherein Group A is a group consisting of 31 specific compounds having a phenolic structure, such as 2,2'-methylenebis(6-tert-butyl-4-methylphenol), 3-hydroxytyrosol, or carnosic acid.

The invention discloses a health-care soft capsule for resisting oxidation and a preparation method of the health-care soft capsule. The soft capsule is prepared from the following components in parts by weight: 1 to 5 parts of natural vegetable oil, 0.5 to 1 part of carnosic acid, 1 to 3 parts of suspending aid, 10 to 20 parts of matrix oil, 8 to 20 parts of gelatin, 0.5 to 2 parts of medical stone water extract, 0.5 to 2 parts of fucoidin, 10 to 25 parts of water, 2 to 8 parts of glycerol, 0.1 to 0.5 part of titanium dioxide and 2 to 5 parts of polyethylene glycol. The natural vegetable oil is selected from at least one of black cumin seed oil, hemp seed oil and yeast cell wall loaded black cumin seed oil. The soft capsule provided by the invention has relatively good oxidation resistance, has the effects of regulating blood fat and resisting inflammation at the same time, and also has relatively good storage stability and disintegration effect.

The invention discloses hair-strengthening and hair loss-preventing shampoo as well as a preparation method and application thereof, and belongs to the technical field of hair-strengthening and hair loss-preventing products, and the hair-strengthening and hair loss-preventing shampoo comprises the following raw materials in parts by weight: 33-45 parts of water; 22 to 41 parts of C14-16 sodium olefin sulfonate; 12 to 25 parts of cocamidopropyl betaine; 18 to 30 parts of sodium lauroyl sarcosinate; 12 to 17 parts of rhus chinensis bark-silk co-extract; 1.5 to 3 parts of carnosic acid; 1 to 6 parts of sodium methyl cocoyl taurate; 1-6 parts of glycerol; 1 to 2 parts of phenoxyethanol; 1 to 2 parts of piroctone olamine salt; 1 to 2 parts of polyquaternium-10; and 1 to 2 parts of disodium EDTA. The components such as the rhus chinensis bark-silk co-extract are added, the rhus chinensis bark contains polyphenol and flavonoid substances, and through experimental detection, the hair-fixing and anti-alopecia shampoo has excellent hair-fixing and anti-alopecia effects.

The invention discloses a Chinese herbal medicine eyesight-improving health-care patch containing a nitric oxide precursor and a preparation process of the Chinese herbal medicine eyesight-improving health-care patch. The Chinese herbal medicine eyesight-improving health-care patch is prepared from the following raw materials in parts by weight: 3 to 6 parts of nitric oxide precursor, 10 to 15 parts of magnetic powder, 15 to 25 parts of porous far infrared ceramic powder, 0.5 to 2 parts of taurine, 0.2 to 0.5 part of musk, 0.1 to 0.3 part of menthol, 1 to 3 parts of frankincense, 0.1 to 0.5 part of wild chrysanthemum flower extract, 0.1 to 0.2 part of carnosic acid and 0.2 to 0.5 part of cassia seed extract. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 0.1 to 0.3 part of flos carthami extract, 0.2 to 0.4 part of semen plantaginis extract, 0.1 to 0.2 part of glutamine, 0.1 to 0.2 part of arginine, 0.1 to 0.3 part of astaxanthin, 0.2 to 0.3 part of taurine, 0.5 to 1 part of borneol and 3 to 5 parts of glycerol. Through the synergistic effect of the magnetic powder, the far-infrared ceramic powder and various raw material medicines, the treatment and relieving effects on eyestrain are remarkably improved, and the preparation is suitable for large-scale popularization and application.

The present invention provides a composite organic fertilizer and its preparation method and application. The composite organic fertilizer includes the following raw materials according to weight: 40-50 parts of basic fertilizer, 5-10 parts of alkaloid compound preparation, 1-3 parts of Dendrobium nobile stem extract, 2-4 parts of carnosic acid nutrient solution, 5-8 parts of composite fermentation liquid and 0.8-1.2 parts of antimicrobial peptide; the alkaloid compound preparation is nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:1-2:0.8-1.2:0.5-0.8:1.2-1.5; the composite fermentation liquid is earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:3-5. The composite organic fertilizer of the present invention is mixed with basic fertilizer by alkaloid compound preparation, Dendrobium nobile stem extract, carnosic acid nutrient solution, composite fermentation liquid and antimicrobial peptide, and has a preventive and therapeutic effect on Phomopsis areca leaf spot disease and banana wilt disease, wherein the banana yield-increasing effect is remarkable, and has good promotion and application value.

A preparation method of a luteolin nano slow-release dosage form belongs to the field of food and medicine, and the specific scheme comprises the following steps: step 1, dissolving luteolin and carnosic acid in a mixed solvent of dichloromethane and methanol to obtain a solution A; step 2, adding the solution A into a polyvinyl alcohol aqueous solution I, and performing ultrasonic treatment to form an emulsion B; step 3, dropwise adding the emulsion B into a polyvinyl alcohol aqueous solution II, and stirring at room temperature to obtain a suspension C; and 4, carrying out centrifugal separation on the suspension C, and washing the precipitate to obtain the luteolin nano stable slow-release dosage form. The luteolin nano stable slow-release dosage form prepared by the invention has the advantages of good water solubility, high loading rate and the like, and can effectively improve the water solubility and slow-release performance of natural micromolecular luteolin in practical application.

The present invention discloses the application of small molecule compounds in the culture of kidney-derived cells. The kidney-derived cells include renal tubular epithelial cells and urine-derived cells from the kidney. The present invention discovers that Corydaline, ARN2966, PKG drug G1, Succinobucol, Meptyldinocap, Rifamycin sodium salt, Carnosic acid, TBHQ, C188-9, Buparvaquone can maintain the long-term culture of primary human renal tubular epithelial cells and improve cell viability. It is also found that Corydaline, ARN2966, and PKG drug G1 have an obvious effect of improving cell viability on urine-derived cells from the kidney, and even a proliferation-promoting effect, which can ensure that a larger number of urine-derived cells can be obtained at an earlier passage.

The invention discloses a pesticide-residue-free antioxidant for inhibiting the activity of apple oxidase and a use process, and belongs to the technical field of fruit postharvest preservation, the pesticide-residue-free antioxidant comprises the following components by mass: 10%-20% of tea polyphenol, 15%-25% of vitamin C, 5%-10% of citric acid, 3%-8% of sodium alginate, 2%-5% of a rosemary extract, and the balance deionized water; the rosemary extract and the sodium alginate are compounded, a wax layer barrier is broken through by means of fat solubility of carnosic acid, a permeation-retention dual mechanism is formed in combination with an ionic cross-linked membrane of the sodium alginate, compared with single tea polyphenol, the skin adsorption amount of active ingredients is increased, thermosensitive ingredients are protected by adopting a low-temperature ultrasonic dissolution technology, and the skin-care effect is improved. Meanwhile, the coordination environment of copper ions in the active center of oxidase is destroyed through accurate pH adjustment.