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Method for extracting antioxidant components from rosmarinus officinalis

An antioxidant ingredient, rosemary technology, applied in the separation/purification of carboxylic acid compounds, organic chemistry, steroids, etc., can solve the problems of large organic solvent consumption, complicated operation, low solvent recovery rate, etc. The effect of stable quality, improving production efficiency and reducing production costs

Active Publication Date: 2019-09-27
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Rosemary extraction methods include solvent extraction method, extraction method, macroporous resin, etc. Solvent extraction and extraction treatment need to consume a lot of organic solvents, high solvent loss rate, low solvent recovery rate, common cumbersome operation, poor process control, etc. The problem is that the yield of the obtained product is low and the product quality is not good
[0004] In the prior art, it is usually aimed at the extraction of one to four components in rosemary. Chinese patent (publication number CN104447334A) discloses a composite extraction process of rosmarinic acid, ursolic acid, oleanolic acid, and carnosic acid. On the one hand, the yield of the extraction process is low, the loss is large, and the product quality is not good
On the other hand, the active ingredients of rosemary cannot be fully extracted, resulting in a waste of rosemary resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 10 kg of rosemary raw materials are pulverized through a 40-mesh sieve with a pulverizer, and then crushed once with a high-pressure homogenizer and added with water, each time for 10 minutes. The product obtained by crushing the homogenizer is placed in the reactor, and when the temperature of the reactor is adjusted to 45° C., 10 grams of cellulase is added (the amount of cellulase is 1 / 1000 of the weight of rosemary), and at 45° C. Enzyme hydrolysis for 1 hour. Put the material obtained by enzymolysis into a flash extractor, use 80% volume fraction ethanol solution as the extraction solvent, the amount of ethanol solution is 18 times the weight of rosemary, the motor speed is 10000R / min, extract at normal temperature for 3 minutes, extract 3 times, One minute at a time. Rest for 2 minutes between each extraction. The filtrate was separated, and the filtrates of the three extractions were combined to obtain the total extract.

[0032] The total extract was concentr...

Embodiment 2

[0037] 10 kilograms of rosemary raw materials are pulverized through a 50-mesh sieve with a pulverizer, and then crushed twice by adding water with a high-pressure homogenizer, each time for 20 minutes. Put the product obtained by crushing the homogenizer into the reaction kettle, and when the temperature of the reaction kettle is adjusted to 46°C, add the plant wall-breaking enzyme (the dosage of the enzyme is 3 / 1000 of the weight of rosemary), and enzymolyze it at 48°C 2 hours. Put the material obtained by enzymolysis into a flash extractor, use 82% volume fraction methanol solution as the extraction solvent, the amount of methanol solution is 20 times the weight of rosemary, the motor speed is 13000R / min, extract at normal temperature for 3 minutes, extract 3 times, One minute at a time. Rest for 2 minutes between each extraction. The filtrate was separated, and the filtrates of the three extractions were combined to obtain the total extract.

[0038] The total extract w...

Embodiment 3

[0043] 10 kilograms of rosemary raw materials are pulverized through a 100-mesh sieve with a pulverizer, and then crushed 3 times with a high-pressure homogenizer, each time for 30 minutes. The homogenizer smashes the gained product and is placed in the reactor, and when the temperature of the reactor is adjusted to 48° C., add cellulose and plant wall-breaking enzymes (the total consumption of the two enzymes is 5 / 1000 of the rosemary weight). Enzyme hydrolysis at 60°C for 3 hours. Enzymolysis gained material is put into flash type extractor, uses 86% volume fraction acetone solution as extraction solvent, and the consumption of acetone solution is 22 times of rosemary weight, motor speed 15000R / min, extract 3 minutes under normal temperature, extract 3 times, One minute at a time. Rest for 2 minutes between each extraction. The filtrate was separated, and the filtrates of the three extractions were combined to obtain the total extract.

[0044] The total extract was conce...

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Abstract

The invention belongs to the field of rosmarinus officinalis extraction, in particular to a method for extracting antioxidant components from rosmarinus officinalis. The method for extracting the antioxidant components from the rosmarinus officinalis comprises the following steps: using a solvent to extract the rosmarinus officinalis, concentrating an extracting solution, centrifuging and collecting a centrifuged supernatant and centrifuged precipitate respectively, and performing macroporous resin purification, concentration and drying on the centrifuged supernatant to separate out rosmarinic acid; dissolving the centrifuged precipitate, using trapped fluid obtained by organic nanofiltration to separate out ursolic acid and oleanolic acid, and using membrane passing liquid obtained by the organic nanofiltration to separate out carnosic acid and carnosol. According to the method provided by the invention, water-soluble and fat-soluble antioxidant components such as the rosmarinic acid, the ursolic acid, the oleanolic acid, the carnosic acid, and the carnosol can be extracted simultaneously, so that the utilization rate of the rosmarinus officinalis is improved.

Description

technical field [0001] The invention belongs to the field of rosemary extraction, in particular to a method for extracting antioxidant components from rosemary. Background technique [0002] Rosemary, Latin scientific name (Rosmarinus officinalis) Lamiaceae shrub. Rosemary is a multi-purpose economic crop, from which antioxidants, rosemary essential oil and other pharmaceutical intermediates can be extracted. Antioxidants are powdery substances extracted from rosemary plants. The main components are antioxidant Functional phenols, acids, flavonoids, etc. Rosemary antioxidant has the characteristics of safety, high efficiency, heat resistance, and good anti-oxidation effect, and can be widely used in daily chemical industries such as tobacco, spices, grain, oil, food, and aquatic products. [0003] Rosemary extraction methods include solvent extraction method, extraction method, macroporous resin, etc. Solvent extraction and extraction treatment need to consume a lot of org...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07D493/08C07C67/48C07C51/42C07C69/732C07C62/32
CPCC07C51/42C07C67/48C07D493/08C07J63/008C07C2603/26C07C69/732C07C62/32
Inventor 赵军杨文国谢永富宋云飞
Owner GUILIN NATURAL INGREDIENTS CORP
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