The invention discloses a Thymus mongolicus Ronn. tissue culture rapid propagation method. Thymus mongolicus Ronn. is a labiatae thymus subshrub plant; the stem and leaves of the Thymus mongolicus Ronn. are aromatic and rich in thymus essential oil; the thymus essential oil mainly contains thymol and carvacrol, and has important application value in the fields of pharmacy, food and cosmetics. The artificial cultivation of the Thymus mongolicus Ronn. mainly refers to culture of seedlings by use of seeds, however, in the culture of seedlings by use of seeds, the seeds are prone to be infected by viruses, and consequently, the properties of the seeds can be degenerated, the yield and the quality are reduced, and the requirements of large-scale and standard production cannot be met. According to the Thymus mongolicus Ronn. tissue culture rapid propagation method, the stem of the Thymus mongolicus Ronn. is taken as an explant, a Thymus mongolicus Ronn. in-vitro regenerated plant is obtained by virtue of processes such as callus induction, multiplication culture, differentiation culture, rooting culture, and acclimatization and transplanting, and an effective tissue culture rapid propagation technical system of the Thymus mongolicus Ronn. is established; as a result, a large quantity of seedlings can be produced in short time, the multiplication efficiency can be improved, and then the breeding and popularization of the Thymus mongolicus Ronn. can be accelerated favorably.