Foam separation method for concentrating and separating rosmarinic acid in perilla leaves

A foam separation method and foam separation technology, applied in the field of rosmarinic acid, can solve the problems of concentration effect and complex operation process in easily polluted environment

Active Publication Date: 2021-09-07
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention overcomes the defects of other separation technologies such as complex operation process, easy pollution of the environment and poor concentration effect

Method used

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  • Foam separation method for concentrating and separating rosmarinic acid in perilla leaves
  • Foam separation method for concentrating and separating rosmarinic acid in perilla leaves
  • Foam separation method for concentrating and separating rosmarinic acid in perilla leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: the preparation of perilla rosmarinic acid concentrate

[0086] The first step, the preparation of perilla leaf extract

[0087] The dried perilla leaves were pulverized by a high-speed pulverizer and passed through an 80-mesh sieve to obtain perilla leaf powder; water was added to the perilla leaf powder according to a material-liquid ratio of 1:100 (g / mL), and the mixture was heated at room temperature and at a speed of 500 r / mL. Stir for 0.2h at a temperature of 90°C and a rotational speed of 60r / min for 1.0h with a rotary evaporator, cool to room temperature and centrifuge at 4000r / min for 15min, take the supernatant and put it in a conditioning tank to obtain perilla Leaf extract, wherein the perilla rosmarinic acid concentration is 246.9mg / L.

[0088] The concentration of rosmarinic acid in perilla is given in "Spectrophotometric Determination of Rosmarinic Acid Content in Perilla Leaves" published by Du Guicai and other researchers on "Journal of Q...

Embodiment 2

[0094] Embodiment 2: the preparation of perilla rosmarinic acid concentrate

[0095] The first step, the preparation of perilla leaf extract

[0096] The dried perilla leaves were pulverized by a high-speed pulverizer and passed through an 80-mesh sieve to obtain perilla leaf powder; water was added to the perilla leaf powder according to a material-liquid ratio of 1:100 (g / mL), and the mixture was heated at room temperature and at a speed of 500 r / mL. Stir for 0.2h at a temperature of 90°C and a rotational speed of 60r / min for 1.0h with a rotary evaporator, cool to room temperature and centrifuge at 4000r / min for 15min, take the supernatant and put it in a conditioning tank to obtain perilla Leaf extract, wherein the perilla rosmarinic acid concentration is 246.9mg / L.

[0097] The second step, the first stage foam separation process

[0098] Add 200 mg / L of cocamidopropyl betaine to the perilla leaf extract in the adjustment tank for the first time, adjust the pH value of t...

Embodiment 3

[0102] Embodiment 3: the preparation of perilla rosmarinic acid concentrate

[0103] The first step, the preparation of perilla leaf extract

[0104] The dried perilla leaves were pulverized by a high-speed pulverizer and passed through an 80-mesh sieve to obtain perilla leaf powder; water was added to the perilla leaf powder according to a material-liquid ratio of 1:100 (g / mL), and the mixture was heated at room temperature and at a speed of 500 r / mL. Stir for 0.2h at a temperature of 90°C and a rotational speed of 60r / min for 1.0h with a rotary evaporator, cool to room temperature and centrifuge at 4000r / min for 15min, take the supernatant and put it in a conditioning tank to obtain perilla Leaf extract, wherein the perilla rosmarinic acid concentration is 246.9mg / L.

[0105] The second step, the first stage foam separation process

[0106] Add 200mg / L of BS12 to the perilla leaf extract in the adjustment tank for the first time, adjust the pH value of this mixed solution ...

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Abstract

The invention provides a foam separation method for concentrating and separating rosmarinic acid in labiatae (preferably perilla leaves). The method comprises the following steps: 1, a perilla leaf extract is prepared; 2, in the first-stage foam separation, a betaine type surfactant is used as an auxiliary agent, rosmarinic acid is effectively collected to a gas-liquid interface, concentration and separation of perilla rosmarinic acid are facilitated, and an obtained concentrated solution can be directly used as a raw material for producing perilla rosmarinic acid powder; and step 3, second-stage foam separation is carried out by taking the first-stage residual liquid as a feed liquid, so that the recovery effect of the rosmarinic acid in the perilla frutescens is further improved. According to the two-stage foam separation, the concentration (enrichment) ratio of the rosmarinic acid can be greatly increased (7.5-14.0), and the recovery rate can be increased (86%-99%). The method has the advantages of greenness in process, no addition of organic reagents, low energy consumption, simplicity in operation and the like, effectively solves the problem of concentration and separation of rosmarinic acid in perilla frutescens, and has good economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of extraction, recovery and concentration of plant active ingredients in a biological separation process, in particular to the concentration and separation of rosmarinic acid in perilla leaves by a two-stage foam separation method. Background technique [0002] Basil (Perilla frutescens L.) is one of the first batch of medicinal and edible plants promulgated by the Ministry of Health of my country, and plays an important role in the construction of general health industry and functional agriculture. In recent years, the demand for perilla in domestic and foreign markets has been increasing year by year: experimental research, writing and writing have sprung up, and introduction, cultivation, and processing and production are in full swing. In particular, perilla leaves are rich in rosmarinic acid (molecular formula C 18 h 16 o 8 ), it is a water-soluble phenolic acid substance, which has strong anti-oxida...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D3/08B01D3/40B01D19/02C11D1/90C11D3/20C11D3/60C07C67/48C07C69/732
CPCB01D3/08B01D3/40B01D19/0413C11D1/90C11D3/2093C07C67/48C07C69/732
Inventor 胡楠张志军李昊哲李会珍李子燚思滢慧
Owner ZHONGBEI UNIV
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