Thymus mongolicus Ronn. tissue culture rapid propagation method

A technology of incense group and culture temperature, applied in the field of plant tissue culture, can solve the problems of wild resources depletion, wild resources can not meet the needs, yield and quality decline, etc., and achieve the effect of improving reproductive efficiency, speeding up breeding and promotion

Inactive Publication Date: 2015-07-22
冯文杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Generally, thyme mainly comes from wild resources. People’s demand for thyme essential oil is increasing day by day. Wild resources cannot meet the needs, and will soon lead to the depletion of wild resources, so artificial cultivation is required.
At present, the artificial cultivation of thyme is mainly seed breeding, but the seedlings are susceptible to virus infection, resulting in the degradation of the species, the decline of yield and quality, and cannot meet the needs of large-scale and standardized production. Therefore, it is necessary to establish a tissue culture technology system for thyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Induction culture: cut the sterilized stem segments into about 0.5 cm, and inoculate them into the induction medium in a flat inoculation method. After inoculation, culture it in the dark, and after the callus appears, move it to the light for 12 hours a day, the light intensity is 1000lx, the culture temperature is 25°C, and the relative air humidity is 75% after 30 days of culture. The induction rate Reached 96.2%. The induction medium is: MS+1.5mg / L KT+0.8mg / L NAA+25g / L sucrose+3.5g / L agar, with a pH of 5.4.

[0019] (2) Proliferation culture: the callus induced by step (1) is transferred to the proliferation medium for propagation. After inoculation, place under the conditions of 12 hours of light per day, light intensity of 2000lx, culture temperature of 25°C, and relative air humidity of 75% for 30 days, and the proliferation coefficient reaches 4.8. The proliferation medium is: MS+0.6mg / L 6-BA+1.5mg / LNAA+25g / L sucrose+3.5g / L agar, with a pH of 5.4.

[0020...

Embodiment 2

[0024] (1) Induction culture: cut the sterilized stem segments into about 0.5 cm, and inoculate them into the induction medium in a flat inoculation method. After inoculation, culture in dark conditions, and then move to place in the light for 14 hours a day when the callus appears, the light intensity is 2000lx, the culture temperature is 27°C, and the relative air humidity is 75% after 30 days of culture and the induction rate Reached 92.8%. The induction medium is: MS+3.5mg / L KT+1.0mg / L NAA+30g / L sucrose+3.5g / L agar, with a pH of 5.4.

[0025] (2) Proliferation culture: the callus induced by step (1) is transferred to the proliferation medium for propagation. After inoculation, place under the conditions of 14 hours of light per day, light intensity of 2000lx, culture temperature of 27°C, and relative air humidity of 75% for 30 days, and the proliferation coefficient reaches 5.1. The proliferation medium is: MS+1.0mg / L 6-BA+2.0mg / LNAA+25g / L sucrose+3.5g / L agar, wi...

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PUM

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Abstract

The invention discloses a Thymus mongolicus Ronn. tissue culture rapid propagation method. Thymus mongolicus Ronn. is a labiatae thymus subshrub plant; the stem and leaves of the Thymus mongolicus Ronn. are aromatic and rich in thymus essential oil; the thymus essential oil mainly contains thymol and carvacrol, and has important application value in the fields of pharmacy, food and cosmetics. The artificial cultivation of the Thymus mongolicus Ronn. mainly refers to culture of seedlings by use of seeds, however, in the culture of seedlings by use of seeds, the seeds are prone to be infected by viruses, and consequently, the properties of the seeds can be degenerated, the yield and the quality are reduced, and the requirements of large-scale and standard production cannot be met. According to the Thymus mongolicus Ronn. tissue culture rapid propagation method, the stem of the Thymus mongolicus Ronn. is taken as an explant, a Thymus mongolicus Ronn. in-vitro regenerated plant is obtained by virtue of processes such as callus induction, multiplication culture, differentiation culture, rooting culture, and acclimatization and transplanting, and an effective tissue culture rapid propagation technical system of the Thymus mongolicus Ronn. is established; as a result, a large quantity of seedlings can be produced in short time, the multiplication efficiency can be improved, and then the breeding and popularization of the Thymus mongolicus Ronn. can be accelerated favorably.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of thyme. Background technique [0002] Thyme ( Thymus mongolicus Ronn. ) Lamiaceae Thyme is a semi-shrub plant, which is widely distributed in northern Africa, Europe and temperate Asia. There are about 300-400 species of Thyme in the world, and there are about 12 species in my country, distributed in Gansu, Shaanxi, Shanxi, Shandong, Hebei, Liaoning and Inner Mongolia and other regions. Thyme stems and leaves have fragrance, rich in thyme essential oil, its main thymol and carvacrol, etc. Studies have confirmed that thyme essential oil and thymol can effectively inhibit the growth of mold and microorganisms in food, and can also relieve nervous tension, and are effective against mosquitoes. Also has the effect of expelling. In addition, thyme also has antioxidant effects. Therefore, thyme ess...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 冯文杰
Owner 冯文杰
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