Test-tube Tilapia nilotica breeding method

A technology of Nile tilapia and fish fry, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of unfavorable large-scale batch production, low reproductive efficiency, and low number of fertilized eggs, and achieve a huge role in promoting and positive meaning, high reproductive efficiency, and high survival rate

Inactive Publication Date: 2011-04-06
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this natural breeding method are: first, the requirements for the breeding pond are high, and it needs to be cleaned and disinfected before the broodstock is put in a suitable place to kill wild fish, harmful organisms and pathogens in the fish pond. Apply basal fertilizer; the second is that the number of fertilized eggs is small, the reproductive efficiency is low, the hatching rate and the survival rate of fry are very low; the third is unfavorable for large-scale batch production, and it is difficult to control artificially; the fourth is that it is difficult to obtain unfertilized eggs and early fertilized eggs , some breeding methods are difficult to apply in the breeding of Nile tilapia
The disadvantage of this method of reproduction is that the timing of ejaculation of tilapia males and the timing of ovulation of tilapia females are not necessarily synchronized
Therefore, the number of fertilized eggs is small and the reproductive efficiency is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Select mature Nile tilapia parents, that is, choose a male fish whose head, body, tail and fins are pink and obvious, weighing 0.4 kg, and have watery milky white semen when artificially squeezed, and select the genital opening The papillae are short and round, and the reddish female fish weighs 0.2 kg and squeezes several eggs manually. The eggs are uniform in size and light yellow in color.

[0032] (2) Inject male and female fish with the high-efficiency fish oxytocic agent for injection produced by Shanghai Tongyi Biotechnology Co., Ltd. The injection dose for female fish is 60IU, and the injection is completed in one injection; the injection dose for male fish is 60IU, and the injection is completed in one injection. The injection sites were all at the base of the pectoral fin.

[0033] (3) After injecting the female and male broodstock, put them into the oxytocin tank for water stimulation. The water temperature is 25°C. After the effect time reaches 25 hours...

Embodiment 2

[0035] (1) Select mature Nile tilapia parents, that is, choose a male fish whose head, body, tail and fins are pink and obvious, weighing 1 kg, and have watery milky white semen when artificially squeezed, and select genital pore milk The protuberance is short and round, and the reddish female fish weighs 0.6 kg and squeezes several eggs manually. The eggs are uniform in size and light yellow in color.

[0036] (2) Two injections with chorionic gonadotropin (HCG). The injection dose for female fish is 480IU, the first injection dose is 160IU, the second injection is 320IU 8 hours after the first injection, and the injection site is the base of the pectoral fin of the female fish; The second injection is a one-time injection; the injection site is the base of the pectoral fin of the male fish.

[0037] (3) After the female and male broodstock were injected with oxytocin drugs, they were put into the oxytocin pool for stimulation with running water. The temperature of the oxyto...

Embodiment 3

[0039] (1) Select mature Nile tilapia parents, that is, choose a male fish whose head, body, tail and fins are pink and obvious, weighing 1 kg, and have watery milky white semen when artificially squeezed, and select genital pore milk Short and round, reddish female weighs 0.6 kg and squeezes several eggs manually. The eggs are uniform in size and light gray in color.

[0040] (2) Carp pituitary gland (PG) + trophic release hormone analogue (LRH-A 2 ) mixed for one injection. The injection dose for female fish is PG 2.4 mg + LRH-A 2 3 micrograms, one injection, the injection site is the base of the pectoral fin of female fish; the injection volume of male fish is PG 2 mg + LRH-A 2 After 2.5 micrograms are mixed, one injection is completed, and the injection site is the base of the pectoral fin of male fish.

[0041](3) After the female and male broodstock are injected with oxytocin drugs, they are put into the oxytocin tank for water stimulation. The temperature of the ox...

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PUM

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Abstract

The invention discloses a test-tube Tilapia nilotica breeding method relating to an aquatic product fry technique and comprising the following steps of: respectively injecting oxytocic into milter and the raun of Tilapia nilotica and then making the milter lay sperms; collecting and then diluting the sperms with 0.8 percent normal saline preserving fluid into 4-5 times and then mixing the sperms with ova laid from the raun and stirring for 3-5 min to fertilize the ova, and then hatching fertilized ova into fries. The test-tube Tilapia nilotica breeding method can greatly improve the breeding efficiency and the hatching rate of the Tilapia nilotica and the survival rate of the fries, and provides the technical and method support for developing the gynogenesis, the polyploid breeding and the family establishment and breeding of the Tilapia nilotica.

Description

technical field [0001] The invention relates to aquatic fry technology, in particular to a method for artificially propagating Nile tilapia. Background technique [0002] Nile tilapia is a tropical fish with wide adaptability, miscellaneous food habits, strong disease resistance, high feed utilization rate, fast growth and tender meat, so it is welcomed by farmers. [0003] At present, the breeding method of Nile tilapia is: put the sexually mature female and male broodstock in the same breeding pond with a water temperature above 18°C, and when the water temperature rises to 22°C or above, the female fish will ovulate and the male fish will ovulate on their own. The fish ejaculate, and the sperm and eggs are combined for fertilization to reproduce naturally. The disadvantages of this natural breeding method are: first, the requirements for the breeding pond are high, and it needs to be cleaned and disinfected before the broodstock is put in a suitable place to kill wild fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 梁宏伟罗相忠李忠邹桂伟杨永铨
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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