33 results about "Matus ovatus" patented technology

The invention relates to cultivation gynogenetic fish, in particular to a method for cultivating gynogenetic carps. The method comprises the following steps of: performing artificial induced spawning on male megalobrama amblycephala and female ordinary carps which serve as male and female parent fish, mixing megalobrama amblycephala sperms inactivated by ultraviolet rays and mature ova of the ordinary carps, activating for 1 to 2 minutes, performing cold shock on the activated ova at the temperature of between 0 and 4 DEG C for 30 to 40 minutes, and incubating embryos obtained by the cold shock at the water temperature of between 20 and 21 DEG C to obtain diploid gynogenetic carps. The gynogenetic carps cultivated by the method have excellent descendant properties, high survival rate and stable inheritance, and have the great significance for the study on biological genetic breeding.

The invention discloses a breeding method for amphiprotic fertile autotetraploid crucian carps and an establishment method for strains of the crucian carps. The breeding method includes the following steps that allotetraploid crucian carps are selected from crucian carp hybridization F1 offspring with red crucian carps as female parents and megalobrama amblycephala as male parents, female individuals capable of generating ova of different sizes and male individuals capable of generating watery semen are selected from the allotetraploid crucian carps for hasten parturition, insemination is carried out manually through a dry method, running water incubation is conducted on fertilized ova, then the fertilized ova are bred, and the amphiprotic fertile autotetraploid crucian carps are selected from selfing offspring. Selfing is carried out on the male individuals and the female individuals in the obtained autotetraploid crucian carps, and the strains of the amphiprotic fertile autotetraploid crucian carps can be established and obtained through multi-offspring breeding. Ploid hybridization is conducted on the autotetraploid crucian carp colonies obtained through the breeding method and diploid crucian carps, so allotriploid fishes can be bred on a large scale. The strains of the crucian carps obtained through the breeding method are excellent in character, stable in heredity and high in fertilization rate and hatchability, and the breeding method has a great significance in genetic research of fishes.

The invention discloses a method for artificially inducing sexual glands of Anguilla marmorata to develop maturely. The method comprises the following steps of: accelerating the maturation of male Anguilla marmorata by intramuscularly injecting human chorionic gonadotropin (HCG), wherein the dosage is 500U/kg based on the body weight of the male Anguilla marmorata, the HCG is injected once every week, and the injection time is 6 weeks; and accelerating the maturation of female Anguilla marmorata by intramuscularly injecting luteinizing hormone releasing hormone A2 (LHRH-A2) and carp pituitaryextract (CPE) homogenate, the dosage of the LHRH-A2 is 250mu g/kg based on the body weight of the female Anguilla marmorata, the dosage of CPE is 4 grains/kg based on the body weight of the female Anguilla marmorata, the LHRH-A2 and the CPE are injected once every 7 to 9 days, and the injection times are 15 to 18. By the scheme, sexual glands of parent fish are developed maturely, abdomens are remarkably protuberant, and the sexual glands are remarkably in a spongy state when convex parts of the abdomens are touched by hands; the female Anguilla marmorata can release mature eggs; and milky white semens can be collected by extruding the abdomens of the male Anguilla marmorata. The method provides a preliminary technical assurance for further researching the completely artificial propagation of the Anguilla marmorata.

The invention discloses an artificial spawning induction method for rhinogobio ventralis. According to the method, the male and female proportion of mature parent fishes is 1:1.5; spawning induction medicine is lutropin releasing hormone analogies, and the dosage of the medicine is 6 micrograms per kilogram of fish weight; according to an artificial fertilization method, female fishes awaiting spawning are caught, moisture of fish bodies are wiped to be dry, ova are extruded into a dry container, then male fishes awaiting spawning are caught, genital openings are wiped to be dry, seminal fluid is sucked up through an injector, placed in a sperm dilution and then mixed with the ova, activation is performed by clear water, standing is performed for two minutes, and then fertilized ova are poured into an incubator for incubation; the flow speed of water bodies in the incubator before stripping of the fertilized ova is 0.4 m/s, the flow speed after stripping is 0.2 m/s, after stripping, the fertilized ova grow in the incubator until fish fry swim bladders are inflated, and then fish fry cultivation can be performed. The spawning induction rate is larger than 65%, the fertilization rate is larger than 85%, the incubation rate is larger than 80%, a large number of fish fries can be provided for batch production of the fish fries of the specification of rhinogobio ventralis, and therefore proliferation liberation of the rhinogobio ventralis is achieved.

The invention discloses a preparation method of young mollusks of Hongkong tetraploid oysters. The method comprises the following technical steps of selectively choosing triploid eggs, carefully choosing diploid sperms, inhibiting second polar bodies of oosperms, obtaining tetraploid young mollusks, and raising tetraploid progenies. The feature of normal reduction mitosis of some triploid eggs is adopted so as to create a raising method for preparing tetraploid oysters by inhibiting second polar bodies of oosperms (triploid eggs +diploid sperms). The method differs from a method for obtaining tetraploid oysters by inhibiting first polar bodies of oosperms. The preparation method makes a great difference in a traditional preparation mode and helps break the monopolies of the US, France, Korea and other foreign countries on raisins technology of tetraploid oysters. It is the first time to obtain livable young mollusks of Hongkong tetraploid oysters. The method makes the biological method of producing 100% Hongkong triploid oysters feasible, promote the steady development of the industry of Hongkong oysters in the coastal region of southern China and offers the possibility of achieving improved varieties of the industry. The preparation method of young mollusks of Hongkong tetraploid oysters is being advantaged by being implementable, reusable, and applicable.

The invention relates to a haliotis discus hannnai and haliotis iris interspecific hybrid production method with a higher fertility rate and a higher hatching rate. According to the interspecific hybrid production method, Chinese haliotis discus hannnai is taken as female parent abalone, and Zelanian haliotis iris is taken as father parent abalone; the female parent abalone and the father parent abalone are put into different parent abalone cultivation ponds for cultivation; the female haliotis discus hannnai with the gonad fully developed is subjected to spawning induction with a method combining shady drying and ultraviolet irradiation seawater stimulation until the female haliotis discus hannnai lays eggs, and the male Zelanian haliotis iris with the gonad fully developed is subjected to spawning induction with a method combining shady drying and hydrogen peroxide seawater soaking until the male Zelanian haliotis iris releases sperms; fertilized eggs of haliotis discus hannnai and haliotis iris hybrids are obtained in the following steps: the eggs of the haliotis discus hannnai and the sperms of the haliotis iris are taken respectively and mixed, the temperature of seawater where fertilization is performed is adjusted, and the eggs are washed after the fertilized eggs are split to two cell stages; the haliotis discus hannnai and haliotis iris hybrid fries can be obtained with conventional hatching and later cultivation methods.

The invention discloses a method for breeding crucian. The method comprises the following steps of establishment of a breeding system; parent fish breeding and nutrient strengthening; arrangement of spawning nests; spawning and collection of fertilized eggs; hatching of the fertilized eggs; larva fish breeding; fry breeding. By means of the method for breeding the crucians, the fry develop well and are robust in physical constitution, ova or parent fishes cannot be damaged during spawning, the fertilization rate of the ova is high, and the survival rate of larva fishes is greatly increased.

The invention discloses a megalobrama amblycephala breeding method. The breeding method comprises the following steps of a, inactivation of heterogeneous male fish sperms, wherein the heterogeneous male fish sperms which are obviously weak in activity, not dead and inactivated are prepared; b, activation of megalobrama amblycephala eggs, wherein the inactivated heterogeneous male fish sperms and the megalobrama amblycephala eggs are subjected to fertilization so as to obtain activated megalobrama amblycephala eggs; c, diploid induced activation of the megalobrama amblycephala eggs, wherein (i)or (ii) is selected; according to (i) cold shock induction, the activated megalobrama amblycephala eggs start to be subjected to cold shock treatment for 10-30 min in the environment at the temperature of 0-4 DEG C 0-6 min after activation; according to (ii) hydrostatic pressure induction, the activated megalobrama amblycephala eggs start to be subjected to hydrostatic pressure shock treatment for 1.5-5 min at the pressure of 40-50 MPa 0-6 min after activation; d, the diploid induced megalobrama amblycephala eggs obtained in step c are incubated so as to obtain megalobrama amblycephala with gynogenesis.

The invention discloses an inducing method of a Nibea albiflora gynogenesis diploid. The technical scheme of the invention is that the inducing method comprises the steps of genetic inactivation of sperms of Nibea albiflora and diploidzation of ova of the Nibea albiflora; the inducing method is mainly characterized by firstly enabling the sperms of the Nibea albiflora to be subjected to the genetic inactivation by using ultraviolet irradiation, then activating the sperms and the ova by using seawater after the sperms are mixed with the ova of the Nibea albiflora, starting gynogenesis of the ova of the Nibea albiflora, restraining the discharge of a second polar body by using cold shock, doubling genomes of the ova, and inducing the Nibea albiflora gynogenesis diploid. The inducing method disclosed by the invention has the advantages that the operation is simple, the needed cost is lower, the practicability is stronger, and the like.

The invention discloses a method for inducing the gynogenesis of megalobrama amblycephala by koi sperm, which roughly comprises the following steps: obtaining a mature ovum and the koi semen of the megalobrama amblycephala by artificial spawning, inactivating the koi semen with a 18-20W ultraviolet lamp for 26 to 30 min, then mixing with the ovum of the megalobrama amblycephala, activating the eggin normal temperature water for 2 to 3 min, then performing cold treatment in cold water at 4 to 6 DEG C for 20 to 22 min, performing running water incubation in normal temperature water, and continuing cultivation to obtain the megalobrama amblycephala in gynogenesis. The koi sperm is adopted to induce the gynogenesis of the megalobrama amblycephala, a megalobrama amblycephala offspring in gynogenesis is obtained by successful breeding, and the growth rate, the meat quality, the stress resistance and the hypoxia tolerance are obviously stronger than those of common megalobrama amblycephala during a breeding process. The method has obvious advantages in breeding, which not only provides a good foundation for improving germplasm resources of the megalobrama amblycephala, but also has greatsignificance in genetic breeding and biological evolution.

A method for breeding first-filial generation commodity spats by hybridizing female Alaska scallops and male Japanese scallops is characterized in that firstly, multiple half-sibling hybridized colonies of female Alaska scallops and male Japanese scallops and respective self-reproducing colonies are respectively built; optimal hybridized colonies are selected, and their corresponding parent self-reproducing colonies respectively become female Alaska scallop breed production colonies and male Japanese scallop breed production colonies for commercial reproduction; after sexual maturity of the scallops is reached, ova of the female Alaska scallop breed production colonies and sperms of the male Japanese scallop breed production colonies are hybridized to reproduce first-filial generation commodity spats. The obtained first-filial generation commodity spats have the advantages of the two scallops, are quick to grow, wide in adaptation and good in stress resistance, are suitable for breeding in sea areas in the in northern China, the yield of the spats is improved by more than 20% compared with Japanese scallops, the survival rate is improved by about 10%, and it is hoped that the method is widely applied in the future.

The application relates to a hybrid seedlings-cultivating method of the stichopus japonicus. The method comprises the steps of: firstly taking the stichopus japonicus in the China sea area and the black stichopus japonicus in the Japan northern sea area as the bred stichopus japonicus, and synchronously fertilizing the bred stichopus japonicus of the China stichopus japonicus and the bred stichopus japonicus of the Japan stichopus japonicus by controlling the cultivating water temperature and the bait feeding quantity of the bred stichopus japonicus in the process of artificial fertilizing and cultivating; artificially spawning and discharging sperm under the oxytocic condition of drying in the shade and warming, and respectively collecting the ovum from the Chinese stichopus japonicus and the sperm from the Japan black stichopus japonicus or the sperm from the Chinese stichopus japonicus and the ovum from the Japan black stichopus japonicus for the aritificial exomixis; and performing the incubation and the seedlings cultivation to the oosperm which is generated by hybridizing the two different geographical colonies which are reproductively isolated from each other according to the normal mode for cultivating the seedlings of the stichopus japonicus so as to cultivate the hybrid stichopus japonicus seedlings.

The invention relates to cultivation of economic brown seaweed and in particular relates to a method for preserving sargassum horneri fertilized eggs at low temperature. The method comprises the following steps: collecting eggs and sperms, which are synchronously discharged by economic seaweed of sargassum horneri, within 2-3 days after fertilization, and preserving the collected fertilized eggs in seawater at the temperature of 4-8 DEG C under 2000 lux. With the adoption of the method, the development process of the fertilized eggs can be greatly delayed, and trans-regional transport of the fertilized eggs in different places is finished before rhizoid elongation, so that seed production can be carried out.

The invention belongs to the technical field of marine organisms, particularly a method for carrying out interspecies cross artificial insemination on an egg of paralichthys olivaceus and a semen of paralichthys dentatus, which is frozen for preservation. The semen of paralichthys dentatus, which is frozen for preservation at an ultralow temperature for a long time, is dissolved in water by two steps; after the semen of paralichthys dentatus is dissolved, diluent of which the volume is three times of that of the frozen semen of paralichthys dentatus is added into the frozen semen of paralichthys dentatus; after the frozen semen of paralichthys dentatus and the diluent are mixed, centrifugation is carried out for 5min; then supernate is removed to obtain frozen semen suspension with the same volume with the frozen semen of paralichthys dentatus before the frozen semen of paralichthys dentatus is diluted; the frozen semen and natural seawater are uniformly mixed according to the proportion of a volume ratio of 1:40 and the mixture is activated; after the mixture is activated, the egg of paralichthys olivaceus is injected and the mixture and the egg of paralichthys olivaceus are slightly and uniformly mixed to enable the egg of paralichthys olivaceus and the frozen semen of paralichthys dentatus to be sufficiently in contact; standing is carried out for 5 to 10min and egg washing is carried out to obtain a fertilized egg to carry out incubation. The invention establishes a cryopreservation technology for semen of paralichthys dentatus and a method for interspecies cross artificial insemination on the egg of paralichthys olivaceus, overcomes the difficulty of asynchrony of development of parental fishes of paralichthys dentatus and paralichthys olivaceus and provides a novel method and a novel idea for seawater fish cross breeding.

The invention belongs to the technical field of aquaculture, and particularly relates to a method for testing the fecundity of holothuria scabra. The method comprises the implementation steps of holothuria scabra treating, weighing and dissecting, sampling, wall breaking and counting; fecundity calculating, wherein the absolute fecundity and relative fecundity of holothuria scabra are calculated according to the following formulas respectively: absolute fecundity=1/2*We*(N1/W1+N2/W2), and relative fecundity=1/2*We/Wt*(N1/W1+N2/W2). The simple method is utilized, a small branch tube wrapping eggs is broken through a dissecting needle, most of the eggs are released out of a tube cavity, and therefore the eggs can be counted under a microscope, and the fecundity of holothuria scabra is tested. The method is easy to operate, simple and feasible, serves development of artificial reproduction and production of holothuria scabra and can provide reference for testing the fecundity of sea cucumbers of other types.

The invention discloses a penaeus vannamei boone half-sib family construction method. The method comprises the steps: selecting mature parent shrimps, marking an individual parent shrimp, setting a mating scheme, acquiring spermatophore, activating sperms, laying eggs by female shrimps, collecting eggs, performing artificial insemination, hatching germ cells, collecting nauplius, and the like. Compared with the traditional artificial spermatophore transplanting method, the method disclosed by the invention has the advantages that no harm on the female shrimp is produced, and a plurality of half-sib families can be simultaneously established; the sperms are artificially released into an egg laying water body, so that the weakness of no mating or separation of the mated female shrimp spermatophore caused by the natural mating can be avoided; on the premise of a set inbreeding coefficient, the mating scheme is artificially designed, so that the inbreeding depression of the constructed family can be effectively avoided, and the method is a high-efficient penaeus vannamei boone half-sib family construction method.

The invention discloses a method for improving the hybridization fertilization rate of female fugu obscurus and male fugu rubripes. According to the method, a fugu obscurus female parent and a fugu rubripe male parent, which are large in size, healthy and sexually mature, are mainly adopted as breeding parents, sperms and ova are thoroughly mixed by a dry method after once pre-ripening physiological regulation, and the sperms and the ova are combined by adjusting osmotic pressure, so that the hybridization fertilization rate of the fugu obscurus female parent and the fugu rubripe male parent is improved, and the method has important reference significance for improving the hybridization fertilization rate of freshwater fishes and marine fishes.

The invention provides an oreochromis niloticus and siniperca chuatsi gynogenesis method. According to the method, gynogenesis is carried out with oreochromis niloticus as female parents and sinipercachuatsi as male parents, in the process, no ocytocic needs to be injected to the oreochromis niloticus and the siniperca chuatsi, siniperca chuatsi sperms do not need to be inactivated, zygotes do not need to be subjected to diploidization, after oreochromis niloticus ova and the siniperca chuatsi sperms are fertilized, about 10% of individuals can open mouths to eat, and it is found through flowcytometry detection ploidy that about 2-3% of individuals are diploid oreochromis niloticus, and the rest are haploids. As the haploids have serious malformation, the haploids can naturally die in the following culture process, the diploid individuals do not need to be screened again, and oreochromis niloticus diploid individuals obtained after gynogenesis can be obtained.