33 results about "Matus ovatus" patented technology

The invention relates to a method for breeding a novel hybrid scallop variety of Argopecten purpuratus and Argopecten irradians irradians. The method comprises the following steps of: establishing a plurality of Argopecten purpuratus and Argopecten irradians irradians hybrid first filial generation families by utilizing the fertilization of eggs of the Argopecten purpuratus and sperms of the Argopecten irradians irradians, selecting excellent hybrid families according to phenotypes of the hybrid families, selecting excellent androgynous individuals from the excellent hybrid families, accelerating maturity, and self-fertilizing respectively to establish a plurality of self-fertilization families; selecting excellent families from the self-fertilization families in the next year, further selecting excellent androgynous individuals from the excellent families and self-fertilizing, and selecting; and continuously self-fertilizing more than three generations by the same method so as to obtain the novel hybrid scallop variety of the Argopecten purpuratus and the Argopecten irradians irradians which has stable inheritance, is large in individuals and wide in a temperature tolerance rangeand can grow quickly. The novel variety can also be realized by a population breeding method. The novel hybrid scallop variety of the Argopecten purpuratus and the Argopecten irradians irradians synchronously has excellent characteristics of the Argopecten irradians irradians and the Argopecten purpuratus, so the novel variety can be cultivated in northern sea areas such as Tsingtao, Dalian and the like; and the yield is improved by over 30 percent compared with that of the Argopecten irradians irradians.

The invention discloses a cultivation method of a gynogenesis megalobrama amblycephala, which comprises the following steps of: firstly selecting and breeding a female megalobrama amblycephala and a male erythroculter ilishaeformis bleeker as parent fishes; then carrying out artificial induced spawning to obtain the sperms of the erythroculter ilishaeformis bleeker and the ripe ovums of the megalobrama amblycephala; mixing the erythroculter ilishaeformis bleeker sperms inactivated by ultraviolet rays with the ripe ovums of the megalobrama amblycephala; 3-4 minutes later, placing the ovums activated at 0-4 DEG C for cold shock treatment for 20-30 minutes; and after incubating embryos treated by cold shock at 23-24 DEG C, obtaining diploid gynogenesis megalobrama amblycephalas through a flow type cell DNA content determination method and a chromosome ploidy detection method of peripheral blood cell culturing. The cultivation method of the gynogenesis megalobrama amblycephala has the advantages of short cultivation time, fast growth speed, high incubation rate, excellent offspring property, and the like, and has great importance on aspects of fish inheritance breeding and biological evolution researches.

An artificial breeding and culturing method for horseshoe crabs includes: mixing eggs of horseshoe crabs with bodily fluid containing sperms, and allowing the mixture to stand until fertilization; putting the fertilized eggs into filtered seawater to be incubated, and filling oxygen until the eggs are incubated into one-year-old horseshoe crabs; breeding the one-year-old horseshoe crabs at the temperature of from 28 DEG C to 30 DEG C until the crabs slough and develop into two-year-old horseshoe crabs; breeding the two-year-old horseshoe crabs at the temperature of from 20 DEG C to 28 DEG C, and using an air pump to fill oxygen; taking out the two-year-old horseshoe crabs and placing into another container containing bait regularly each day, changing the filtered seawater once a day until the crabs are bred into five-year-old crabs; breeding the horseshoe crabs which are five years old or of different ages at the temperature of from 20 DEG C to 28 DEG C, and filling oxygen; taking out the crabs and breeding in another container containing bait regularly each day, and changing the filtered seawater once a day; and taking out the crabs in breeding and breeding in a container or cement pit with oyster fragments until the crabs are bred into adult horseshoe crabs.