33 results about "Matus ovatus" patented technology

The invention relates to a method for breeding a novel hybrid scallop variety of Argopecten purpuratus and Argopecten irradians irradians. The method comprises the following steps of: establishing a plurality of Argopecten purpuratus and Argopecten irradians irradians hybrid first filial generation families by utilizing the fertilization of eggs of the Argopecten purpuratus and sperms of the Argopecten irradians irradians, selecting excellent hybrid families according to phenotypes of the hybrid families, selecting excellent androgynous individuals from the excellent hybrid families, accelerating maturity, and self-fertilizing respectively to establish a plurality of self-fertilization families; selecting excellent families from the self-fertilization families in the next year, further selecting excellent androgynous individuals from the excellent families and self-fertilizing, and selecting; and continuously self-fertilizing more than three generations by the same method so as to obtain the novel hybrid scallop variety of the Argopecten purpuratus and the Argopecten irradians irradians which has stable inheritance, is large in individuals and wide in a temperature tolerance rangeand can grow quickly. The novel variety can also be realized by a population breeding method. The novel hybrid scallop variety of the Argopecten purpuratus and the Argopecten irradians irradians synchronously has excellent characteristics of the Argopecten irradians irradians and the Argopecten purpuratus, so the novel variety can be cultivated in northern sea areas such as Tsingtao, Dalian and the like; and the yield is improved by over 30 percent compared with that of the Argopecten irradians irradians.

The invention discloses a cultivation method of a gynogenesis megalobrama amblycephala, which comprises the following steps of: firstly selecting and breeding a female megalobrama amblycephala and a male erythroculter ilishaeformis bleeker as parent fishes; then carrying out artificial induced spawning to obtain the sperms of the erythroculter ilishaeformis bleeker and the ripe ovums of the megalobrama amblycephala; mixing the erythroculter ilishaeformis bleeker sperms inactivated by ultraviolet rays with the ripe ovums of the megalobrama amblycephala; 3-4 minutes later, placing the ovums activated at 0-4 DEG C for cold shock treatment for 20-30 minutes; and after incubating embryos treated by cold shock at 23-24 DEG C, obtaining diploid gynogenesis megalobrama amblycephalas through a flow type cell DNA content determination method and a chromosome ploidy detection method of peripheral blood cell culturing. The cultivation method of the gynogenesis megalobrama amblycephala has the advantages of short cultivation time, fast growth speed, high incubation rate, excellent offspring property, and the like, and has great importance on aspects of fish inheritance breeding and biological evolution researches.

The invention provides a cultivation method for hybridized all-female golden trout fries. An androgen is utilized to induce golden trout parent fish in a mature period so as to obtain golden trout pseudo-milter, steelhead parent fish eggs are fertilized with golden trout pseudo-milter sperms, hatching is performed, and the hybridized all-female golden trout fries are obtained. The eyed rate of the fertilized eggs during a hatching period is over 81%, the fry hatching rate is over 92%, the female rate of the obtained fries is 100% by detection, the rate of golden trout (with golden yellow body color) of the fries is over 90%, the weight gaining rate is over 122% after the fries are cultivated for 30 days, and the survival rate is over 98%. Compared with fries generated by purebred golden trout mating, the cultivation method for the hybridized all-female golden trout fries maintains the golden trout rate to be unchanged, enables the golden trout fries having double advantages of being hybridized and all-female to be obtained, and has good economic benefit and market value.

The invention discloses a method for artificially inducing sexual glands of Anguilla marmorata to develop maturely. The method comprises the following steps of: accelerating the maturation of male Anguilla marmorata by intramuscularly injecting human chorionic gonadotropin (HCG), wherein the dosage is 500U / kg based on the body weight of the male Anguilla marmorata, the HCG is injected once every week, and the injection time is 6 weeks; and accelerating the maturation of female Anguilla marmorata by intramuscularly injecting luteinizing hormone releasing hormone A2 (LHRH-A2) and carp pituitaryextract (CPE) homogenate, the dosage of the LHRH-A2 is 250mu g / kg based on the body weight of the female Anguilla marmorata, the dosage of CPE is 4 grains / kg based on the body weight of the female Anguilla marmorata, the LHRH-A2 and the CPE are injected once every 7 to 9 days, and the injection times are 15 to 18. By the scheme, sexual glands of parent fish are developed maturely, abdomens are remarkably protuberant, and the sexual glands are remarkably in a spongy state when convex parts of the abdomens are touched by hands; the female Anguilla marmorata can release mature eggs; and milky white semens can be collected by extruding the abdomens of the male Anguilla marmorata. The method provides a preliminary technical assurance for further researching the completely artificial propagation of the Anguilla marmorata.

The invention belongs to the field of aquaculture, and particularly relates to an artificial control method for inducing the synchronous maturing of the gonads of male paralichthys dentatus parent and female paralichthys olivaceus parent. In the method, the male paralichthys dentatus parent is subjected to full-illumination environmental control in autumn and winter to suppress the gonad development of the male paralichthys dentatus parent; and in the spring of the next year, the full-illumination environment is changed into short-illumination environment, and the male paralichthys dentatus parent and the female paralichthys olivaceus parent are subjected to gonad development control at the same time to realize the gonad maturing of the male paralichthys dentatus parent. The method provided by the invention can obtain mature sperms and ova at the same time, prolongs the germ production period of the male paralichthys dentatus parent, increases the sperm quantity, and lays a technological base for obtaining batch high-quality sperms and ova in the large-scale offspring seed production of hybrid flounder.

An artificial breeding and culturing method for horseshoe crabs includes: mixing eggs of horseshoe crabs with bodily fluid containing sperms, and allowing the mixture to stand until fertilization; putting the fertilized eggs into filtered seawater to be incubated, and filling oxygen until the eggs are incubated into one-year-old horseshoe crabs; breeding the one-year-old horseshoe crabs at the temperature of from 28 DEG C to 30 DEG C until the crabs slough and develop into two-year-old horseshoe crabs; breeding the two-year-old horseshoe crabs at the temperature of from 20 DEG C to 28 DEG C, and using an air pump to fill oxygen; taking out the two-year-old horseshoe crabs and placing into another container containing bait regularly each day, changing the filtered seawater once a day until the crabs are bred into five-year-old crabs; breeding the horseshoe crabs which are five years old or of different ages at the temperature of from 20 DEG C to 28 DEG C, and filling oxygen; taking out the crabs and breeding in another container containing bait regularly each day, and changing the filtered seawater once a day; and taking out the crabs in breeding and breeding in a container or cement pit with oyster fragments until the crabs are bred into adult horseshoe crabs.

The invention relates to the rearing of economic brown algae, in particular to a preservation method of fertilized eggs and embryos of sargassum fusiforme, which specifically comprises the following steps: culturing male and female parents of sargassum fusiforme under the conditions that the light intensity is 10000-50000 lux, the photoperiod is 12-16 hours, and the temperature is 18-24 DEG C, replacing water within 24 hours once, and culturing for 7-10 days, so that the parents synchronously discharge eggs and sperms; and collecting the fertilized eggs within 12 to 24 hours after the parents synchronously discharge the eggs and the sperms, completing the collection of the fertilized eggs and the embryos at a temperature of 0 to 8 DEG C within 8-16 hours after fertilization, and then preserving. The invention utilizes the low-temperature method to culture the eggs in an appropriate culture medium within 8-16 hours after the sargassum fusiforme eggs are fertilized, and then preserves the fertilized eggs and the embryos, thereby substantially delaying growth process, so that the cross-regional transport is completed before rhizoids are not formed so as to carry out seed rearing.