Method for carrying out interspecies cross artificial insemination on frozen semen of paralichthys dentatus and paralichthys olivaceus

Abstract

The invention belongs to the technical field of marine organisms, particularly a method for carrying out interspecies cross artificial insemination on an egg of paralichthys olivaceus and a semen of paralichthys dentatus, which is frozen for preservation. The semen of paralichthys dentatus, which is frozen for preservation at an ultralow temperature for a long time, is dissolved in water by two steps; after the semen of paralichthys dentatus is dissolved, diluent of which the volume is three times of that of the frozen semen of paralichthys dentatus is added into the frozen semen of paralichthys dentatus; after the frozen semen of paralichthys dentatus and the diluent are mixed, centrifugation is carried out for 5min; then supernate is removed to obtain frozen semen suspension with the same volume with the frozen semen of paralichthys dentatus before the frozen semen of paralichthys dentatus is diluted; the frozen semen and natural seawater are uniformly mixed according to the proportion of a volume ratio of 1:40 and the mixture is activated; after the mixture is activated, the egg of paralichthys olivaceus is injected and the mixture and the egg of paralichthys olivaceus are slightly and uniformly mixed to enable the egg of paralichthys olivaceus and the frozen semen of paralichthys dentatus to be sufficiently in contact; standing is carried out for 5 to 10min and egg washing is carried out to obtain a fertilized egg to carry out incubation. The invention establishes a cryopreservation technology for semen of paralichthys dentatus and a method for interspecies cross artificial insemination on the egg of paralichthys olivaceus, overcomes the difficulty of asynchrony of development of parental fishes of paralichthys dentatus and paralichthys olivaceus and provides a novel method and a novel idea for seawater fish cross breeding.

Description

technical field

[0001] The invention belongs to the technical field of marine organisms, and specifically relates to an artificial insemination method for interspecies hybridization of flounder ova and cryopreserved flounder sperm. Background technique

[0002] Cross-breeding is a breeding method that is relatively easy to implement and effective. It uses existing varieties or species to carry out crossbreeding, and selects a gene combination that meets people's needs from its offspring. Selecting geographically distant species as hybrid parents can increase the polymorphism of hybrid progeny, and it is easy to obtain a variety of types from their hybrid progeny, and it is relatively easy to obtain new types that meet the requirements. Flounder (Paralichthys olivaceus) and summer flounder (Paralichthys dentatus) are important marine fish species, which are distributed in the Pacific and Atlantic coasts respectively. Although they belong to the same genus, their geographical ...