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Artificial breeding and culturing method for horseshoe crabs

A breeding method and artificial technology, applied in the field of artificial seedling and breeding, can solve problems such as the inability to apply artificial seedlings, and achieve the effect of huge economic and social benefits

Inactive Publication Date: 2013-09-04
洪水根
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] Relevant artificial insemination of horseshoe crab has been reported, and the applicant (Hong Honggen. Chinese horseshoe crab embryo development research [J]. Xiamen University Journal (Natural Science Edition), 2002, 41 (2)) has reported the relevant Chinese horseshoe crab embryo development research, these The research is mainly suitable for laboratory research on the characteristics and laws of the horseshoe crab embryo process, and cannot be applied to large-scale artificial breeding to solve the problem of seedlings
[0020] Up to now, there is no report of artificial breeding of horseshoe crab at home and abroad

Method used

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  • Artificial breeding and culturing method for horseshoe crabs
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  • Artificial breeding and culturing method for horseshoe crabs

Examples

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Embodiment Construction

[0049] The following examples further illustrate the present invention.

[0050] 1. Artificial breeding

[0051] 1. The choice of pro-Limulus

[0052] The key to the success of artificial insemination is to choose a good horseshoe crab with good maturity. Scrub the male and female horseshoe crabs with sea water.

[0053] Open the reproductive cover of the abdomen of the female horseshoe crab, and a pair of reproductive holes can be seen. Squeeze the reproductive holes with hands. If mature eggs are discharged, the female horseshoe crab can be retained for insemination.

[0054] Open the genital cover of the male Limulus abdomen, use a syringe to extract hemolymph (containing semen) from the genital opening, drop it on the glass slide, add the same amount of seawater, cover the glass slide, and observe under a high-power microscope to confirm that there are a large amount of blood. The swimming sperm, the male horseshoe crab can be retained. If no motile sperm can be observ...

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Abstract

An artificial breeding and culturing method for horseshoe crabs includes: mixing eggs of horseshoe crabs with bodily fluid containing sperms, and allowing the mixture to stand until fertilization; putting the fertilized eggs into filtered seawater to be incubated, and filling oxygen until the eggs are incubated into one-year-old horseshoe crabs; breeding the one-year-old horseshoe crabs at the temperature of from 28 DEG C to 30 DEG C until the crabs slough and develop into two-year-old horseshoe crabs; breeding the two-year-old horseshoe crabs at the temperature of from 20 DEG C to 28 DEG C, and using an air pump to fill oxygen; taking out the two-year-old horseshoe crabs and placing into another container containing bait regularly each day, changing the filtered seawater once a day until the crabs are bred into five-year-old crabs; breeding the horseshoe crabs which are five years old or of different ages at the temperature of from 20 DEG C to 28 DEG C, and filling oxygen; taking out the crabs and breeding in another container containing bait regularly each day, and changing the filtered seawater once a day; and taking out the crabs in breeding and breeding in a container or cement pit with oyster fragments until the crabs are bred into adult horseshoe crabs.

Description

technical field [0001] The invention relates to an artificial seedling raising and cultivating method, in particular to an artificial seedling raising and cultivating method of horseshoe crab. Background technique [0002] Limulus, also known as horseshoe crab, belongs to Arthropoda, Merostomata, Xiphosurida, and Limuroidea. According to its shape and distribution characteristics, the existing Limulus in the world can be divided into two subfamilies, three genera and four types. Among them, the Limulinae subfamily has only one species, Limulus and Limulus polyphemus, which are distributed in eastern North America. Coastal waters; and Tachypleinae subfamily has two genera, distributed in the southeastern coast of Asia and the coastal waters of the east coast, of which there are two species of Tachypleus: Tachypleus tridentatus (also known as three-sting horseshoe crab, Chinese horseshoe crab or Japanese horseshoe crab) Limulus) and Tachypleus gigas (also called Giant Limulus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 洪水根廖水明孙水火薛文艺
Owner 洪水根
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