A kind of spider incense tissue culture medium and culture method

A spider incense, culture medium technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of limiting the rapid scale of artificial cultivation, low reproduction rate, long reproduction cycle, etc., and achieve high industrial promotion value. , Improve the efficiency of tissue culture and reduce the cost of tissue culture

Active Publication Date: 2022-01-25
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the continuous development and application of the pharmacological effects of spider incense and the development and application of daily chemical products and food, the market demand for spider incense is expanding day by day. However, spider incense is basically supplied to the market by mining wild resources, and limited wild resources have been over-harvested. Digging and the destruction of the ecological environment lead to the shortage of spider incense resources, which limits the application of the spider incense function. At present, only a small amount of cultivation has begun in Yunnan, Guizhou and other places, and the current main propagation method is to dig wild resources for division propagation
On the one hand, the total biomass of wild resources is limited. On the other hand, the reproductive coefficient of ramets is 2-5, and the reproductive rate is low. Generally, the reproductive cycle is 2-3 years. The long reproductive cycle limits the rapid scale of artificial cultivation in a short period of time. change

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Establishment of a sterile system: Take the underground rhizomes of Cinnamomum chinensis, rinse the soil with tap water, rinse with detergent solution for 30 minutes, rinse with tap water 3 times, scrape off the epidermis, rinse with tap water for 80 minutes, transfer to an ultra-clean bench, and rinse with 75 Disinfect with % alcohol for 20 seconds, rinse with sterile water twice, disinfect with 100 mg / L chlorine dioxide solution for 30 minutes, rinse with sterile water for 3 times, disinfect with 0.1% mercury solution + 2 drops of Tween-80 for 10 minutes, rinse with sterile water Wash 7 times.

[0027] 2. Cluster bud induction culture: inoculate the sterilized underground rhizomes with buds in the modified MS+KT 5.0mg / L+IBA 0.01mg / L+activated carbon 0.5g / L+sugar 30g / L+agar 3.8g / L In the induction proliferation medium, the pH was adjusted to 5.8, and the temperature was 25±1°C, the light intensity was 2500lx, and the light intensity was 12h per day, and the culture ...

Embodiment 2

[0033] 1. Establishment of a sterile system: Take the underground rhizomes of Cinnamomum chinensis, rinse the soil with tap water, rinse with detergent solution for 30 minutes, rinse with tap water 3 times, scrape off the epidermis, rinse with tap water for 60 minutes, transfer to an ultra-clean bench, wash with 75 Disinfect with % alcohol for 30 seconds, rinse with sterile water for 3 times, disinfect with 100 mg / L chlorine dioxide solution for 30 minutes, rinse with sterile water for 4 times, disinfect with 0.1% mercuric solution + 2 drops of Tween-80 for 15 minutes, rinse with sterile water Wash 6-8 times.

[0034] 2. Cluster bud induction culture: Inoculate the sterilized underground rhizomes with buds in the improved MS+KT10.0mg / L+IBA 0.2mg / L+activated carbon 0.5g / L+white sugar 30g / L+agar 3.8g / L In the medium for inducing proliferation, the pH was adjusted to 5.8, and the temperature was 25±2°C, the light intensity was 2000 lx, and the light duration was 12 hours per day ...

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PUM

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Abstract

The invention discloses a spider incense tissue culture medium and a culture method, wherein the tissue culture medium has the advantages of simple components, low cost and easy acquisition, can significantly improve the induction rate, proliferation coefficient, and rooting rate of spider incense, and can Effectively improve tissue culture efficiency, reduce production costs, and have market application value.

Description

technical field [0001] The invention relates to the field of tissue culture of traditional Chinese medicines, in particular to a tissue culture medium and a culture method of spider incense. Background technique [0002] Spider incense (Valeriana jatamansi Jones), also known as horseshoe incense, is a perennial herb of the genus Valeriana in the Patrinaceae family, and its rhizomes are used as medicine. It has the effects of regulating qi and relieving pain, eliminating food and diarrhea, dispelling wind and dampness, and calming the nerves. It is mainly used for abdominal distension and pain, food accumulation, diarrhea, dysentery, rheumatic arthralgia, soreness of the waist and knees, and insomnia. It has important medicinal value. resource plants. The volatile oil contained in spider incense has a special aroma, and its main components are isovaleric acid, 3-methylvaleric acid, camphor, borneol, chlorophyllene, oliveene, etc. It is used as an additive for cigarettes, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 张金渝蒋元省赵露琴杨维泽杨绍兵曾祥飞邓清李纪潮余正强沈孝明苏杰
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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