49results about How to "Reduce the cost of tissue culture" patented technology

The invention relates to an open type method for cultivating toxin-free seedlings of sugarcanes, comprising the following steps: processing the stems with hot water; preparing the anti-bacterial agent; disinfecting the ware; inoculating; executing the inducing cultivation, the propagation cultivation and the rooting cultivation; and transplanting from seedling bottles. The open type method for cultivating the toxin-free seedlings of the sugarcanes of the invention needs not to sterilize the culture medium at high temperature, inoculates in a natural and open environment, does not need the clean benches, radically simplifies the cultivation ring, and reduces the cultivation cost. Compared with the traditional cultivation, the open cultivation at least reduces over 20% of cost. Because of simple operation, the method can be implemented after preparing the different culture mediums according to prescriptions and adding the anti-bacterial agent. The investment is little, the practicability is strong, and the popularization is good, so the method can be popularized in sugarcane planters.

The invention relates to a simple tissue culture method for broussonetia papyrifera. The simple tissue culture method for the broussonetia papyrifera comprises the following steps: preparing a bacteriostatic agent, sterilizing a culture vessel, preparing a culture medium, performing induction culture, performing proliferation culture, performing rooting culture and transplanting a bottled plantlet. By the simple tissue culture method for the broussonetia papyrifera, the culture vessel and the culture medium are not required to be sterilized at high temperature and high pressure, so that the workload and the energy consumption are reduced, the tissue culture link of the broussonetia papyrifera is simplified, and tissue culture cost of the broussonetia papyrifera is lowered; the simple tissue culture method for the broussonetia papyrifera is easy to operate, high in practicality and good in popularization, and the culture medium is prepared according to different formulas and the bacteriostatic agent is added; and compared with the conventional tissue culture method for the broussonetia papyrifera, by the simple tissue culture method for the broussonetia papyrifera, the cost can be reduced by more than 10%.

The invention provides a special culture medium for improving the tissue culture propagation speed of haworthia succulent plants and a tissue culture method. The special culture medium is characterized in that the ingredients of the special culture medium include a basic culture medium and low-dosage 2,4-D. The tissue culture method comprises the following steps of 1 explant selection and sterilization, 2 inoculation and aseptic strain establishment and 3 transfer and expanding propagation. The problem of low tissue culture propagation speed of the haworthia succulent plants can be solved, the propagation speed of the haworthia plants is remarkably improved, the cost of the whole tissue culture is reduced, and the marketing speed of products is improved.

The invention belongs to the technical field of plant tissue breeding, and in particular to a method for reducing the aerial root of hydrangea, aiming at solving the problem that the aerial root is generated in the tissue culture process of the hydrangea and improving the survival rate of tissue culture seedling of the hydrangea. The method is realized by the following steps: the tissue culture seedling of the hydrangea is taken as an experimental material and is inoculated in a liquid rooting medium (1/2B5+NAA or IBA 0.1-1.0mg/L+sugar 10g/L pH 4-7) of perlite (40-60ml)+25-35ml; after 30 days, no aerial root is generated in the tissue culture seedling, the rooting rate in a culture flask reaches 100%, and the transplanting survival rate reaches 100%. The method not only improves the transplant survival rate and simplifies the operating procedures of transplantation, but also saves the cost of tissue culture by using the perlite to replace agar and recycling the perlite.

The invention relates to the field of plant tissue culture and discloses a tissue culture method for increasing the survival rate of psidium guajava L.. The tissue culture method comprises the following first step of disinfection of a culture room, the second step of selection of explants, the third step of sterilization of the explants, the fourth step of initial induction culture, the fifth step of subculture, and the sixth step of seedling hardening. According to the method, the explants are disinfected with plant treating liquid, and thus the survival rate of the explants in the tissue culture process is increased; meanwhile, a commonly-used MS culture medium is replaced with cassava alcohol waste liquid, and thus the tissue culture cost can be effectively reduced. The tissue culture method can be applied to various explants of psidium guajava L., the surfaces of the explants can be effectively disinfected without disoperation to the explants, and meanwhile the production cost can be effectively reduced.

The invention discloses a tissue culturing method for regenerating somatic cells of an aquatic iris, belonging to the technical field of plant tissue culturing. The tissue culturing method comprises the steps of: (1) preparing a culturing medium; (2) culturing detoxified tissue culture seedlings of the aquatic iris; (3) transplanting and culturing the tissue culture seedlings; and the like. A tissue culture rapid propagation technical system established by using aseptic seedling leaves as embryoid inducing explants is characterized by high propagation coefficient, high speed, complete structure, high regeneration rate, low production cost and the like; compared with the general organ tissue culturing method, the tissue culturing method for regenerating somatic cells of the aquatic iris has the advantages of shortening the whole culturing period to 75 days, increasing the seedling proliferation coefficient by 2.4 times, reaching a culture seedling rooting rate of 100 percent and a transplanting survival rate of 100 percent, being applicable to industrial seedling culture and realizing commercialized production. The method can be popularized and applied to enterprise production of the aquatic iris.

The invention discloses an anoectochilus formosanus sound seedling culture method. According to the anoectochilus formosanus sound seedling culture method, anoectochilus formosanus seedlings obtainedby tissue culture are transferred into a sound seedling culture medium and are subjected to sound seedling culture for 90 to 100 days; the sound seedling culture medium contains potatoes and waste anoectochilus formosanu leaves and/or stems. By adopting the anoectochilus formosanus sound seedling culture method, the effect of invigorating anoectochilus formosanus seedlings soundings can be remarkably improved and the medical property of anoectochilus formosanus also can be improved; circulating tissue culture is realized and the cultivation cost is reduced.

The invention relates to a sealwort sterile rooting breeding method in a test tube. The method comprises the following steps: (1) sealwort seed bud selection and sterilization; (2) callus induction; (3) subculture proliferation culture; (4) seedling hardening and rooting; (5) sand bed provisional planting. The sealwort sterile rooting breeding method has the beneficial effects that the sealwort seedling culture period is short; a great number of sealwort seedlings can be formed in five months; the tissue culture cost can be greatly reduced by using multiple shoot proliferation and differentiation; the culture period is shortened; the tissue culture sealwort seedling growth condition is regular and uniform; the medicine effect quality is unified; the breeding speed is high; the influence byexternal environmental factors is avoided; the production can be performed in four seasons in one year; the sealwort tissue culture seedlings have uniform properties; the excellent properties of parents and the growth period consistency can be maintained; the purity of the sealwort tissue culture seedlings is higher; the variation is little; the sealwort field standardized production is facilitated.

The invention discloses a culture medium for tissue culture of valeriana jatamansi and a culture method. The culture medium for the tissue culture has the advantages of simple components, low cost andeasiness for obtaining; the induction rate, multiplication coefficient and rooting rate of the valeriana jatamansi can be remarkably improved; the tissue culture efficiency can be effectively improved and the production method is reduced; the culture medium has market-oriented application value.

The invention relates to an antibacterial culture method for genetically-transformed tobacco. The antibacterial culture method comprises the steps of: bacteriostat preparation; culture vessel sterilization; agrobacterium tumefacien transformation; culture medium preparation; co-culture; screening culture; rooting culture; resistive seedling detection. Due to the adoption of the antibacterial culture method for the genetically-transformed tobacco, provided by the invention, a culture vessel and a culture medium are both not needed to be sterilized at high temperature and high pressure, so that the workload and energy consumption are reduced, the culture link of the genetically-transformed tobacco is simplified, and the culture cost of the genetically-transformed tobacco is reduced; the antibacterial culture method for the genetically-transformed tobacco, provided by the invention, is simple in operation, strong in practicability and good in popularization, and only a bacteriostat is added after culture mediums are prepared according to different formulas; in addition, compared with the conventional method, the antibacterial culture method for the genetically-transformed tobacco can be used for reducing the cost by more than 10%.

The invention discloses a bacteriostatic agent for an open type bacteriostatic culture medium, the open type bacteriostatic culture medium and a preparation method of the open type bacteriostatic culture medium. The bacteriostatic agent for the open type bacteriostatic culture medium comprises 0.1-0.3 g/L of cefixime, 0.1-0.3 g/L of streptomycin, 0.1-0.3 g/L of carbendazim and 0.1-0.3 g/L of mancozeb, wherein the concentration refers to the final concentration during use. The open type bacteriostatic culture medium comprises the bacteriostatic agent and also comprises a basic culture medium and a curing agent. The bacteriostatic agent has a good bacteriostatic effect, the whole operation process is simpler, the tissue culture cost is reduced, indoor operation can be allowed, and the accessthreshold is low. The operation is extensive, promotion in rural areas is facilitated, and mass production is facilitated.

The invention discloses a tissue culture inoculating method for dendrobium officinale. The tissue culture inoculating method for the dendrobium officinale comprises the following steps: selection of dendrobium officinale pods, sowing of seed embryos, preparation of sterile seed embryo mixed liquid, preparation of a germinating and rooting culture medium, and inoculation and culture of the seed embryos. The method disclosed by the invention is a simple and effective tissue culture inoculating method. According to the method, the germinating and rooting culture medium is inoculated to the cultured seed embryos through once induction, so that repeated culture rotation is not required; moreover, the germinating and rooting culture medium prepared in the method disclosed by the invention is mainly based on a natural antibacterial additive, and does not have harmful chemical component additive, so that the problems that the debugging time is long, the workload is long, the pollution rate is high, and the stability of produced seed seedlings is poor in conventional artificial one-by-one inoculation can be effectively solved; the pollution rate in the tissue culture process of the dendrobium officinale can be greatly reduced, and the tissue culture cost of the dendrobium officinale can be reduced; the tissue culture inoculating efficiency and the transplanting survival rate of the dendrobium officinale are greatly improved; the production efficiency is 3 to 4 times that of the conventional method; the transplanting survival rate can reach more than or equal to 92 to 93 percent.

The invention discloses a culture method for prolonging the storage period of kiwi fruit tissue culture seedlings. A certain amount of sucrose is added on the basis of the conventional subculture medium of kiwi fruit tissue culture seedlings, so that the storage time of the tissue culture seedlings can be effectively prolonged. The problem of short storage period of the existing kiwi fruit tissueculture seedlings is solved. Through the storage of the kiwi fruit tissue culture seedlings performed according to the method provided by the invention, the growth of the tissue culture seedlings canbe generally delayed for 4 to 5 months; the survival rate of the tissue culture seedlings can reach 80 percent or higher.

The invention relates to a method for quickly obtaining transgenic eggplant plants and belongs to the technical field of a transgenic eggplant plant obtaining method. In order to solve the problem of low eggplant genetic transformation efficiency, the invention provides the method for quickly obtaining the transgenic eggplant plants, which comprises the following steps: firstly, pre-culturing eggplant explants on a regenerative culture medium, activating agrobacterium tumefaciens carrying cryIIIA genes, infecting the eggplant explants with the agrobacterium tumefaciens and culturing the infected eggplant explants on the regenerative culture medium to transform explant cells with the agrobacterium tumefaciens; then transferring the explants onto a selective culture medium for further culture so as to form adventitious buds and further form the complete plants. The method provided by the invention has the benefits that both the regenerative culture medium and the selective medium contain thiabendron and N6-[isoamene]-adenine, so that the induction efficiency is high, the quantity of the adventitious buds formed by the explants can be increased, and the regeneration efficiency growth rate can reach 163 percent; according to the method provided by the invention, the transformation efficiency of transgenic eggplant breeding is improved, and the transformation period is shortened.