Proliferation and rapid propagation method of green spheroids of ferns submerged intermittently

A technology for green spheroids and ferns, which is applied in the field of proliferation and regeneration of fern green spheroids, can solve the problem that the overall cleaning and sterilization of the aseptic manual inoculation operation device is not easy, and it is not easy to disassemble or move and control components. complex problems, to achieve the effect of high utilization rate of culture space, simple and compact structure, and saving culture space

Active Publication Date: 2017-11-28
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such devices are usually bulky, difficult to disassemble or move, and the control elements are complicated, and the maintenance cost is high, and they are not easy to operate aseptic manual inoculation and efficient overall cleaning and sterilization of the device
[0005] At present, there are only reports on the GGB cultivation of a few ferns such as kidney fern, nest fern, and staghorn fern in China, and the cultivation methods used are all solid cultivation, and there is no report on intermittent immersion cultivation of GGB

Method used

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  • Proliferation and rapid propagation method of green spheroids of ferns submerged intermittently
  • Proliferation and rapid propagation method of green spheroids of ferns submerged intermittently

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1 Pteridophyta GGB proliferates and propagates rapidly and cultivates

[0037] 1. Take fern leaves and rhizomes of mature sporophytes, wash them with running water for 2 hours, soak them in 75% alcohol for 10-20 seconds, sterilize them with HgCl2 for 6 minutes, and wash them with sterile water 4 times to obtain sterile GGB induction materials.

[0038] 2. Put leaves, rhizomes and other materials into the induction medium, about 3-4 weeks, GGB appears on the explants.

[0039] 3. First stack a plurality of empty sieve trays in the fixed frame, then put the empty object component into the main body of the culture container, and then engage the screw thread to seal the cover and the main body of the culture. In addition, the outer opening of the main body of the culture container is sealed with cotton balls, and the whole device is to be sterilized under high temperature and high pressure;

[0040] 4. The silicone hose can be sealed with newspaper and sterilize...

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PUM

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Abstract

The invention discloses a method for multiplying and rapidly multiplying fern green spheroids by intermittent immersion, which comprises the following steps: 1) aseptically culturing to obtain fern green spheroids; 2) making the green spheroids obtained in step 1) The body is placed in a connecter-type intermittent plant tissue culture device for intermittent immersion culture. The present invention has the advantages of high utilization rate of cultivation space and favorable exchange of cultivation gas components, thereby improving culture quality and reproduction coefficient, suitable for large-scale production; cultivation device and accessories are easy to be sterilized as a whole, the cultivation process is completely closed, and the probability of pollution is small; The structure is simple and compact, with low energy consumption, which saves the culture space and reduces the cost of tissue culture; it reduces the transfer and subculture operation of tissue culture seedlings in the overall tissue culture process, and saves labor and production costs.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method suitable for the proliferation and regeneration of fern green globular bodies (Green Globular Body, GGB). Background technique [0002] Plant tissue culture technology has the advantages of large and fast reproduction, easy control of experimental conditions, continuous production, and high production efficiency. It is widely used in crop breeding, germplasm resource preservation, and virus-free seedlings of flowers, fruit trees, vegetables, forest trees, and Chinese herbal medicines. Production and rapid propagation and vegetative propagation, such as the vegetative rapid propagation of lily virus-free seed balls. [0003] Traditional solid or semi-solid tissue culture methods consume a large amount of agar, and a large number of containers need to be handled during filling and cleaning, which consumes a lot of labor and leads to high production costs. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李杨石雷柴敏李东
Owner INST OF BOTANY CHINESE ACAD OF SCI
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