Proliferation and rapid propagation method of green spheroids of ferns submerged intermittently

A technology for green spheroids and ferns, which is applied in the field of proliferation and regeneration of fern green spheroids, can solve the problem that the overall cleaning and sterilization of the aseptic manual inoculation operation device is not easy, and it is not easy to disassemble or move and control components. complex problems, to achieve the effect of high utilization rate of culture space, simple and compact structure, and saving culture space
CN105210867BActive Publication Date: 2017-11-28INST OF BOTANY CHINESE ACAD OF SCI
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF BOTANY CHINESE ACAD OF SCI
Publication Date
2017-11-28

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention discloses a method for multiplying and rapidly multiplying fern green spheroids by intermittent immersion, which comprises the following steps: 1) aseptically culturing to obtain fern green spheroids; 2) making the green spheroids obtained in step 1) The body is placed in a connecter-type intermittent plant tissue culture device for intermittent immersion culture. The present invention has the advantages of high utilization rate of cultivation space and favorable exchange of cultivation gas components, thereby improving culture quality and reproduction coefficient, suitable for large-scale production; cultivation device and accessories are easy to be sterilized as a whole, the cultivation process is completely closed, and the probability of pollution is small; The structure is simple and compact, with low energy consumption, which saves the culture space and reduces the cost of tissue culture; it reduces the transfer and subculture operation of tissue culture seedlings in the overall tissue culture process, and saves labor and production costs.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and relates to a method suitable for the proliferation and regeneration of fern green globular bodies (Green Globular Body, GGB). Background technique

[0002] Plant tissue culture technology has the advantages of large and fast reproduction, easy control of experimental conditions, continuous production, and high production efficiency. It is widely used in crop breeding, germplasm resource preservation, and virus-free seedlings of flowers, fruit trees, vegetables, forest trees, and Chinese herbal medicines. Production and rapid propagation and vegetative propagation, such as the vegetative rapid propagation of lily virus-free seed balls.

[0003] Traditional solid or semi-solid tissue culture methods consume a large amount of agar, and a large number of containers need to be handled during filling and cleaning, which consumes a lot of labor and leads to high production costs. [00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More