160 results about "Actinia" patented technology

The invention discloses a variety breeding and early-season high-yield cultivation technology for actinidia arguta. The variety breeding and early-season high-yield cultivation technology includes procedures of 1 variety selection, 2 seedling propagation, 3 field planting, 4 underground management, 5 pest control and 6 pruning. Seedling propagation carried out in a step 2 includes collecting and storing cuttings and performing root cutting on the cuttings in sunlight greenhouses, particularly, root cutting is performed on the cuttings in the nutrient pots, and greenwood cutting is performed on the cuttings. Compared with the prior art, the variety breeding and early-season high-yield cultivation technology has the advantages that seedlings are raised in the sunlight greenhouses by means of root cutting, so that the seedling growth time can be prolonged, the seedlings are healthy and are high in growth potential after being planted, and a foundation is laid for early and high yields; frame surfaces are replaced by small canopy frames, and accordingly operation and management can be facilitated.

The invention discloses a grafting method for a kiwi tree, comprising the following steps: the trunk of a kiwi tree with the tree age more than 5 years is sawn off in the period of the end of November and the middle of December of the first year, the kiwi tree grows to the middle of the second year, the trunk of the kiwi tree is sawn off to reach the height of 100-120 cm, scions from a robust kiwi tree are selected and grafted onto the kiwi tree with the sawn-off trunk, grafting incisions are bandaged, and the grafting is finished. Through the grafting method, the disadvantage that a main stem branch is poor in renewability existing in the grafting of kiwi trees with the tree age more than 5 years is solved, the replacement pace of tree spices is accelerated, the trees are quick in recovering growth vigor, the diseases are less, and the fruiting capacity of cultured branch groups is strong.

The invention provides a method of rapid breeding of kiwi fruit with large seed by tissue culture. The invention comprises steps of selecting and treating explant, inducing culture medium, root culturing and replanting, etc. The invention takes MS culture medium as basis and adds BA, NAA, GA, ZT and other external plant growth regulator to induce the growth of axillary bud of the said kiwi fruit and strengthen sprout and root. The root growth rate is as high as 100%, the average bud height is 6.28 cm and the average replanting survival rate is 91% after 35 days culture. The invention solves the technique problem of rapid breeding of said kiwi fruit and provides a method of short period, high breeding rate and low cost for the industrial production of kiwi fruit with large seed.

The invention discloses a secondary grafting method of hongyang kiwifruit. The secondary grafting method includes: cultivating first-grade grafting stock actinidia valvata; grafting actinidia valvata and a wild kiwifruit variety, and growing seedlings; grafting hongyang kiwifruit at a high position of the wild kiwifruit after growing for one year for cultivation. By adopting the method, the hongyang kiwifruit is high in adaptability and can normally grown in places different in altitude, terrain, soil and river valley and flat ground, so that kiwifruit cultivation area can be widened. The hongyang kiwifruit is high in yield, large and regular in fruit shape, orderly in size and good in taste, and the problem that actinidia valvata and the hongyang kiwifruit are low in affinity and grafting survival rate is solved.

The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for establishing a kiwifruit in vitro regeneration system, which comprises: the steps of selecting and sterilizing explants, the step of inducing adventitious buds, the step of multiplying adventitious buds, the step of inducing rooting, and Seedling hardening and transplanting steps. According to the method for establishing the kiwifruit in vitro regeneration system provided by the present invention, the axillary bud induction rate reaches 100%, the leaf callus induction rate reaches 92%, the germination rate is 40%, the adventitious bud proliferation coefficient is 4.5, and the rooting rate can reach 96%. Not only can the kiwifruit reproduce rapidly, in large quantities and with high quality, but also the leaf callus induction step can provide a basis for the study of kiwifruit genetic transformation.

The invention belongs to the fields of artificial cultivation and molecular biology of A. arguta, and particularly relates to a molecular marker for early identification of sex of A. arguta young seedlings and an application of the molecular marker. Most of A. arguta belongs to dioecious plants, the economic value of female plants is apparently higher than that of male plants, in the breeding course, the juvenile stage of tree seedlings is excessively long, and male plants and female plants can be distinguished out by naked eyes after 5-7 years, so that if the sex of the young seedlings can beidentified at the early stage, massive breeding cost can be saved. The invention provides the molecular marker for early sex identification of tree seedlings of the A. arguta, and based on the molecular marker, a reagent kit for auxiliary breeding for the sex of the A. arguta and an early identification method for the sex of the A. arguta are established. Through detection on 95 A. arguta singleplants of which the sex is known, the testing accuracy rate can reach 95.7%. The molecular marker and the method disclosed by the invention are good in generality, simple to operate, good in stabilityand good in application prospects.

The invention discloses lotus young-keeping noodles, which are prepared from the following raw materials in part by weight: 100 to 150 parts of flour, 5 to 8 parts of buckwheat, 4 to 8 parts of konjac flour, 3 to 5 parts of lotus pollen, 5 to 8 parts of needle mushroom powder, 3 to 5 parts of acerola cherry freeze-dried powder, 5 to 8 parts of kiwi fruit freeze-dried powder, 1 to 2 parts of roselle calyx, 3 to 5 parts of cassia seed, 1 to 2 parts of osmanthus fragrans, 3 to 5 parts of Lecai, 2 to 4 parts of Chinese wolfberry, 2 to 4 parts of Chinese angelica, 5 to 7 parts of common lophatherum herb, 3 to 5 parts of lotus leaf, 2 to 4 parts of lotus, 1 to 3 parts of salt and 0.1 to 0.3 part of grape seed oil. The lotus young-keeping noodles are good in mouthfeel, delicious and rich in nutrition and simultaneously have dietetic and health-care effects of maintaining beauty and keeping young.

The invention discloses a tissue culture and rapid propagation method for actinidia chinensis, and relates to the tissue culture and rapid propagation method for actinidia chinensis seedlings. According to the method, young leaves of the current year serve as explants and are inoculated on a medium MS+0.3mg/LZT+0.05mg/LNAA subjected to callus induction and cluster bud differentiation. The invention also provides an improved MS; after cluster buds are differentiated, the cluster buds are sub-cultured in an improved MS+2mg/LZT proliferation culture medium, and are transferred into an improved MS+0.015mg/LTDZ thickening culture medium according to seedling height; small seedlings which are in accordance with rooting are transferred into 1/2 of improved MS+0.7mg/LIBA+0.1g/LAC rooting culture medium; the small seedlings are transplanted and are domesticated after roots grow. By the method, the callus induction rate reaches 96.4 percent; the height of cluster bud reaches 1.67cm; the proliferation coefficient of cluster buds reaches 3.67; the rooting rate reaches 98.12 percent; moreover, the culture medium is low in cost and short in propagation period, and industrial production can be realized.

The invention relates to the field of plant pollen preservation and in-vitro culture vitality detection and particularly relates to a preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta. The method comprises the steps: picking flower buds of the Actinidia arguta at a big bud stage and taking out anthers; drying the anthers, and carrying out screening, so as to collect pollen; sealing the collected pollen, and carrying out cryopreservation at the temperature of -85 DEG C to -75 DEG C; taking out the cryo-preserved pollen, and rewarming the cryo-preserved pollen at room temperature; uniformly spreading the rewarmed pollen onto a solid culture medium, carrying out culturing, and then, carrying out microscopy under a microscope, and counting the germinating rate of the pollen. According to the preservation method, the vitality of the pollen is still preserved good after the pollen is preserved for a long time, so that the pollen in-vitro culture detection method can be applied to the vitality detection on the pollen of the Actinidia arguta and is simple, rapid and accurate.

The invention discloses a bacteria strain of antagonistic plant pathogenic bacteria and application of the bacteria strain. The antagonistic bacteria are Paenibacillus profundus XZQ-20, and the microbial preservation number of the Paenibacillus profundus XZQ-20 is CGMCC No. 12556. The Paenibacillus profundus XZQ-20 is separated from rhizosphere soil of poplar, can produce protease and inhibit the growth of plant pathogenic bacteria such as Lonsdalea quercina, Pseudomonas syringae, Erwinia carotovora and Xanthomonas juglandis, and can be used for preventing and treating plant diseases caused by the plant pathogenic bacteria above. The antagonistic bacteria with biological preventing and treating potential are high in preventing and treating efficiency, wide in preventing and treating range, good in environment safety and promising in development and application prospect.

The invention discloses a real-time fluorescence PCR method for quantitatively detecting Pseudomonas syringae pv. Actinidiae. The method comprises the following steps: extracting an Actinidia chinensis sample genome DNA; electrophoretically detecting the band size and purity of the Actinidia chinensis sample genome DNA; carrying out fluorescent quantitative detection on a PSA pathogen; carrying out specific primer-based fluorescent quantitative amplification, and preliminarily determining whether the PSA pathogen is contained or not according to an amplification curve; purifying a PCR product with an obvious amplification peak, and sequencing the purified PCR product; and comparing a sequence obtained after the sequencing by Internet to further determine the PSA pathogen. The method allows a fluorescent quantitative PCR-based rapid detection method of the pathogen to be established, realizes rapid identification of the Pseudomonas syringae pv. Actinidiae infected sample, has the characteristics of simplicity in operation, high sensitivity, high specificity, and accurate and objective detection result, and is of great significance to improve the detection efficiency and the detection sensitivity of the Pseudomonas syringae pv. Actinidiae infected sample.

The invention discloses a method of cultivating Actinidia arguta, comprising the steps of selecting a variety, constructing a yard, improving soil, pruning, managing fertilizer and moisture, controlling disease and pest damage and the like; the method has the advantages that seedling cost is decreased, management cost is decreased, fertilizer utilization rate is increased, and the yield and quality of Actinidia arguta are significantly increased.

The invention discloses a method for recognizing the contribution proportion of kiwi fruit hybrid patients on a filial generation genome. The method comprises the following steps of performing genome low-depth sequence testing on hybrid parents and filial generations and distant hybrid outgroup; performing sequence testing data reference genome comparison, and obtaining single basic group variation information; performing single basic group variation-based genome window division and log probability estimation; performing sub window maximum possible gene tree building and confidence interval estimation; performing gene tree level and filial generation genetic relationship statistics and genome contribution ratio prediction on the hybrid parents. The whole genome variation information is used for analyzing the evolution genetic relationship of the hybrid patients and the hybrid filial generation, so that the prediction of the hybrid filial generation characteristic properties realizes high accuracy; meanwhile, by using the method, the magnitude and the direction of the possibly existing phenotypic characteristic variation are predicted in the baby period of the hybrid filial generation formation; the early stage screening of the hybrid strains can be greatly promoted; the labor and material cost can be reduced; the resource mining and utilization efficiency can be greatly improved.

The invention discloses a culture medium for subculture multiplication of tissue cultured seedlings of red-flesh kiwifruits. On the basis of MS culture medium, through adjusting the types and concentrations of major elements and optimizing the concentration of hormone regulation agar and the like, balanced and reasonable nutritional requirement is provided to the subculture multiplication of the tissue cultured seedlings of the red-flesh kiwifruits, the rapid, efficient and strong growth of the red-flesh kiwifruits is ensured, the inter-node growth is effectively promoted and the multiplication coefficient is improved.

The invention relates to the technical field of microorganisms and particularly relates to a pseudomonas strain for preventing and treating kiwifruit canker and application of the pseudomonas strain. The Pseudomonas (pseudomonas sp.) strain provided by the invention has a preservation number of CCTCC NO: M2016556. The pseudomonas is obtained by separating and purifying collected kiwifruit twigs, a separation and screening method is simple, and the strain has very strong inhibition effect to pathogenic bacteria of kiwifruit bacterial canker and is capable of obviously inhibiting the growth of the pathogenic bacteria of the kiwifruit bacterial canker. The strain is separated from healthy plant tissues and is inoculated to the kiwifruit twigs, leaves are not abnormal, and a generated active metabolite has no obvious toxic effect to the plant tissues; the strain has stable property and remarkable effects on treating and preventing the kiwifruit canker and is easily wetted through spraying and beneficial to the environmental protection, and an environment-friendly, simple and effectively way is provided for the control and treatment of the kiwifruit canker.

By means of constituting Anthopleura sp tentacle cDNA library and DNA sequencing and designing degenerate primer based on the end conservative amino acid sequences of actinia neurotoxin protein, one new kind of actinia polypeptide neurotoxin gene, named su, is separated via RT-PCR process. The new gene has length of 141 bp and encodes 47 amino acid mature peptides. The expressed protein has ioelectric point of 7.9 and molecular weight of 4800 dalton. Cloning su in the trxA gene 3' end of thioredoxin gene fusion expression vector pETTRX, fusion gene is constituted and the fusion gene exists incolibacillus in intracellar soluble form with expression amount up to 100-120 mg/L. Through Ni-Chelating affinity chromatographic analysis, proteinase 3C cutting and gel filtering, recombinant actinia neurotoxin protein with purity over 95% is obtained with the yield of 10-50 mg/L.

The invention discloses a strong seedling rooting culture medium for kiwi fruits. The strong seedling rooting culture medium is characterized by comprising 1/2 improved MS, 0.5-1.0mg/L of BA, 0.3-0.7mg/L of NAA, 3-5g/L of agar and 20g/L of sucrose; the pH value is 5.8. According to the strong seedling rooting culture medium for the kiwi fruits, components and contents are improved and culture conditions are adjusted so that the transplanting survival rate of the kiwi fruits can be more than 90%; sprouts grow healthily; the output and the quality of the kiwi fruits can be effectively improved and the economic values are enhanced.

The invention provides a kiwi fruit tree grafting method. The method comprises the following steps that a trunk of a red-centred kiwi fruit tree with the tree age larger than four is sawn off, when the tree grows to the middle of the second year, the trunk is sawn to reach the height of 100-120 cm, a scion is selected from a robust wild actinidia eriantha tree for grafting, and a cut is bound finally; the cut of the red-centred kiwi fruit tree serving as a stock is treated with repairing liquid, and a scion branch is soaked in a plant growth regulator. According to the method, the grafting survival rate is high, grafted kiwi fruits are enlarged, and the character is stable.

The present invention discloses a method for grafting kiwi, comprising the steps of: S1, rootstock selection year old seedlings, root, no pests, in diameter is 0.4-0.8, stems smooth body, upright growth robust plants selected drained pots, charcoal and stones placed in the pelvic floor, then put sand in the bowl, then the rootstock planted in pots, choose varieties of pure, robust growth, bud full full, no pests annual spring shoots of branches, for the spring scion generally combined collection winter pruning, trimming down the selected branches, hang tab, 50-100 branches bunch, wet sand storage, summer and autumn for budding of scions; S2, January grafted spring mid to late February, summer grafting in mid-June to early July.The present invention is novel, unique measures, the process is simple, easy to implement, does not require investment in infrastructure, low investment, the effect is significant, it can be worth promoting the application.

The invention relates to a preparation method of fruit vinegar fermented from Lucid Ganoderma. The preparation method comprises the following steps: 1, selecting 70% of Lucid Ganoderma (wall broken Lucid Ganoderma) and 30% of millet, fermenting by a traditional fermentation process, extracting a fermentation broth; 2, mixing 20% of a Pyrus serotina juice, 20% of an apple juice, 20% of a sea buckthorn juice, 20% of an Actinidia chinensis juice and 20% of a tomato juice to obtain a mixed juice; 3, taking 500kg of purified water, adding 4% of the fermentation broth, 4% of the mixed juice and uniformly stirring according to a preparation proportion of 92%; and 4, adding relevant accessories, uniformly mixing, sterilizing, canning, sealing, cooling, and packaging. So the fruit vinegar is obtained.

The invention discloses a banana actinidia japonica rice jam. The jam is prepared from the following raw materials in parts by weight: 190-220 parts of bananas, 60-70 parts of actinidia, 70-80 parts of japonica rice, 14-18 parts of apple flowers, 8-10 parts of endothelium corneum, 2-3 parts of alpinia officinarum hance, 4-6 parts of mesona blume, 5-8 parts of mosla chinensis maxim, 3-4 parts of chaenomeles pip, 4-5 parts of Chinese chestnut leaves, 52-56 parts of white granulated sugar, 22-26 parts of konjaku flour, 2-4 parts of green tea powders, 10-13 parts of sesame oil, 26-30 parts of Chinese date honey and appropriate amount of purified water. The japonica rice with a health care effect is added in the preparation process of the jam disclosed by the invention; the mesona blume can stop bleeding by astringency, stop dysentery, and kill worm; the endothelium corneum can disperse accumulation and stagnation, and strengthen the spleen and the stomach; the alpinia officinarum hance can warm the stomach for dispelling cold, help digestion and kill pain; the chaenomeles pip can get rid of pathogenic fire; the mosla chinensis maxim can induce perspiration, relieve summer heat, promote water circulation, disperse moisture, warm the stomach and regulate middle energizer; and the jam is bright and beautiful in color, moderate in sweet and sour, delicate and soft in taste, and high in nutrition and health care value, and has unique fruit aroma and japonica rice aroma and wide market prospect.