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Method for quickly in-vitro actinidia kolomikta propagating

A kind of fast technology of kiwifruit, applied in the field of in vitro rapid propagation of kiwifruit, can solve the problems of difficulty in producing a large number of seedlings, difficult inoculation and survival of kiwifruit, and the excellent asexual characters of the seedlings cannot be completely preserved, so as to improve the transplanting effect. The effect of survival rate, saving seedling cost and reducing seedling cost

Inactive Publication Date: 2013-01-02
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a rapid in vitro propagation technology of kiwifruit to solve the technical problems that kiwifruit inoculation is difficult to survive, it is difficult to produce a large number of seedlings in a short period of time, and the excellent asexual properties of seedlings cannot be completely preserved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of culture medium:

[0026] 1) Basic medium: prepared by conventional MS medium formula, in which the concentration of agar is 7g / L, white sugar is used to replace sucrose, the concentration is 28 g / L, and the pH value of the basic medium is adjusted to 5.8;

[0027] 2) Induction medium: add 6-BA or 6-benzylaminopurine to the basic medium to make the concentration 1.0mg / L;

[0028] 3) Subculture medium: add 6-BA, 6-benzylaminopurine, to the basic medium to make its concentration 1.8mg / L, NAA, or naphthaleneacetic acid, to make its concentration 0.8 mg / L and IBA, or indole Butyric acid, so that its concentration is 1.25mg / L;

[0029] (2) Selection and disinfection of explants:

[0030] 1) Selection of explants: select the detached leaves of mature plants of Actinidia chinensis as explants;

[0031] 2) Disinfection of explants: Rinse the selected leaves with clean water for 1.5 hours, disinfect them with 70% alcohol on an ultra-clean workbench for 30 s...

Embodiment 2

[0037] (1) Preparation of culture medium:

[0038] 1) Basic medium: prepared by conventional MS medium formula, in which the agar concentration is 7.5g / L, white sugar is used to replace sucrose, the concentration is 25 g / L, and the pH value of the basic medium is adjusted to 5.5;

[0039] 2) Induction medium: add 6-BA or 6-benzylaminopurine to the basic medium to make the concentration 1.5mg / L;

[0040] 3) Subculture medium: Add 6-BA, 6-benzylaminopurine, to the basic medium to make the concentration 2.0mg / L, NAA, naphthaleneacetic acid, to make the concentration 1.0mg / L and IBA, indole Butyric acid, so that its concentration is 1.0mg / L.

[0041] (2) Selection and disinfection of explants:

[0042] 1) Selection of explants: select the detached leaves of mature plants of Actinidia chinensis as explants;

[0043] 2) Disinfection of explants: Rinse the selected leaves with water for 1 hour, sterilize with 70% alcohol for 30 seconds on an ultra-clean workbench, then rinse 3 ti...

Embodiment 3

[0049] (1) Preparation of culture medium:

[0050] 1) Basic medium: prepared by conventional MS medium formula, in which the agar concentration is 6.5g / L, sucrose is replaced with white sugar, the concentration is 30g / L, and the pH value of the basic medium is adjusted to 5.8;

[0051] 2) Induction medium: add 6-BA or 6-benzylaminopurine to the basic medium to make the concentration 0.5mg / L;

[0052] 3) Subculture medium: Add 6-BA, 6-benzylaminopurine, to 1.5mg / L, NAA, naphthaleneacetic acid, to 0.5mg / L, and IBA, indolebutyric acid, to the basic medium , so that the concentration is 1.5mg / L.

[0053] (2) Selection and disinfection of explants:

[0054] 1) Selection of explants: select the detached leaves of mature plants of Actinidia chinensis as explants;

[0055] 2) Disinfection of explants: Rinse the selected leaves with water for 2 hours, disinfect them with 70% alcohol for 30 seconds on an ultra-clean workbench, then rinse them three times with sterile water, and disi...

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Abstract

The invention belongs to the technical field of plant tissue culture and the field of forest propagation, and in particular relates to a method for quickly in-vitro actinidia kolomikta propagating. The method is mainly and technically characterized by comprising the following steps of: medium preparation; explant selection and disinfection; induction culture; subculture and rooting culture; and tissue culture seedling transplantation and acclimatization. The method has the beneficial effects that the adopted leaf blade is an explant which is convenient and easy to obtain, and the industrialized production throughout the year can be realized through only using a small amount of test materials; the production period is short, 53-65 days are only spent from inoculation to tissue culture seedling, the blade inductivity can reach 93%, the multiplication times can reach 12-15, and the transplanting rooting ratio can reach 92%; the provided method does not need to root in a bottle but adopts root dipping outside the bottle, not only can the time for rooting in the bottle be saved, but also the seedling cost is lowered; and by utilizing the provided method, the rooting ratio is increased during seedling culture in winter, the transplanting survival rate is increased and can reach 95%, and simultaneously the seedling culture cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and the field of forest breeding, in particular to a rapid in vitro propagation method of kiwi fruit. Background technique [0002] Dog date kiwi fruit ( Actinidia kolomikta (Maxim. et Rupr.) Maxim. ) belongs to Actinidiaceae, Actinidia genus. Jujube kiwi fruit is soft and juicy, sweet and sour, fragrant, and has high nutritional value. It is rich in vitamin C (Vc), organic acids, protein, fat, various sugars, pectin, triterpenoids, flavonoids, Sugar, volatile oil and a large amount of free amino acids and other components; its chemical composition is of great value in medicine, scientific research, food, greening, industry and other industries, and is a natural resource with great development and utilization prospects. In particular, it has anti-mutation, anti-distortion and anti-tumor effects, which have attracted great attention from scholars at home and abroad. Vigorously expl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘长江孙晓荣谭昌华潘松杨玉红
Owner SHENYANG AGRI UNIV
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