5 results about "Actinidia chinensis" patented technology

This invention provides a Psa disease resistance gene and its application. Specifically, this invention demonstrates through genetic and molecular experiments that the Psa disease resistance gene is effective against the hardy kiwifruit (Actinidia arguta). Actinidia arguta Wild germplasm Aa11251 containing the G932 protein sequence triggered an immune response upon injection of the HopAO2 effector protein from *Actinidia chinensis*, while materials lacking the G932 protein did not. Transient co-expression experiments on the model plant *Nicotiana benthamiana* showed that co-expression of the resistance protein G932 and the effector protein triggered an HR immune response in the plant. Simultaneously, in *Actinidia chinensis* (Chinese kiwifruit...),... A. chinensis The stable expression of G932 in the transgenic 'Donghong' variety significantly enhances its resistance to pathogens. This indicates that the G932 protein and its encoding gene can be used in agricultural production for the breeding of resistant kiwifruit varieties to bacterial canker. This invention is of great significance for the breeding of disease-resistant kiwifruit varieties and can be used in agricultural production.

The application provides a method for efficiently breeding Actinidia chinensis, comprising the following steps: taking leaves or stem segments of Actinidia chinensis as explants, and performing culture treatment including induction culture on the explants to obtain Actinidia seedlings; wherein an induction culture medium used in the induction culture comprises an MS culture medium and an additive, the additive is selected from at least two of KT, 2,4-D, 6-BA, NAA, IBA and IAA; and in the culture medium, the content of the KT is 0.05-0.1 mg / L, the content of the 2,4-D is 0.5-1.0 mg / L, the content of the 6-BA is 0.1-0.5 mg / L, the content of the NAA is 0.5-1.0 mg / L, the content of the IBA is 0.01-0.05 mg / L, and the content of the IAA is 0.5-1.0 mg / L. The method provided by the application overcomes the influence of environmental factors on the breeding of Actinidia chinensis, and helps the protection of biodiversity.

The present application relates to the technical field of plant molecular biology, and proposes a kiwi drought-resistant gene AcNFXL1 and application thereof.A kiwi drought-resistant gene AcNFXL1 The nucleotide sequence of the kiwi drought-resistant gene AcNFXL1 is shown as SEQ ID NO.1.Through the above technical solution, the problem of poor drought resistance of kiwi in the prior art is solved.

This invention discloses a *Rummeliibacillus* sp. and its application, relating to the field of microbial technology. The *Rummeliibacillus* sp., named *Rummeliibacillus* JY-2-4R, was deposited on February 11, 2026, at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession number GDMCC No: 67853. This invention applies the strain to the preparation of preparations antagonistic to *Actinidia chinensis* black spot, *Actinidia chinensis* soft rot, *Actinidia arvensis* white rot, *Actinidia arvensis* soft rot, or *Actinidia arvensis* root rot. The *Rummeliibacillus* JY-2-4R of this invention is a potential biological agent possessing stress resistance, growth promotion, and broad-spectrum antibacterial activity, with broad development potential and good application prospects, addressing the problem of a lack of functional microorganisms for the biological control of kiwifruit and *Actinidia arvensis* diseases.

This invention belongs to the field of traditional Chinese medicine quality testing technology, specifically disclosing a method for constructing a characteristic chromatogram and determining the content of *Actinidia chinensis* root. The method for constructing the characteristic chromatogram includes the following steps: S1: Take *Actinidia chinensis* root as a reference material, add 70% methanol for ultrasonic extraction, filter, and obtain a reference solution; separately take catechin, epicatechin, proanthocyanidin B1, and proanthocyanidin B2 reference standards, add 70% methanol to prepare a mixed reference solution; S2: Take *Actinidia chinensis* root powder, add 70% methanol for ultrasonic extraction, add weight, filter, and obtain the final product; S3: Inject the samples separately, record the chromatograms, and construct the characteristic chromatogram. This invention establishes a characteristic chromatogram of *Actinidia chinensis* root using high-performance liquid chromatography (HPLC) and simultaneously determines the content of four components: proanthocyanidin B1, catechin, proanthocyanidin B2, and epicatechin, achieving comprehensive, accurate, and stable quality control of *Actinidia chinensis* root.