20 results about "Actinidia chinensis" patented technology

The invention provides a composition with a soothing effect, a preparation scheme of the composition and application of cosmetics. The composition comprises the following components in percentage by mass: 35-50% of actinidia chinensis fruit water, 2-10% of a radix gentianae extract, 1-9% of a citron fruit extract, 1-4% of a dendrobium stem extract, 1-5% of an oat extract and the balance of butanediol. All the components in the composition cooperate with one another, the multi-dimensional synergistic effect of resisting inflammation, resisting histamine, repairing a barrier, resisting oxidation and adjusting micro-ecology can be achieved, and the composition has the excellent relieving effect.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for early identification of the cold resistance of an actinidia chinensis tree body in the dormancy period, and the SNP molecular marker is located at the Chr69297479 position on the No.6 chromosome of the actinidia chinensis and is closely associated with the cold-resistant phenotype median lethal temperature (LT50) of the actinidia chinensis. The method can be used for identifying the tree body dormancy period cold resistance of natural population and hybrid filial generation of actinidia chinensis. The homozygous genotype (CC) of the SNP locus corresponds to a high-cold-resistance individual in the dormancy period of an actinidia chinensis tree body, and the heterozygous genotype (TC) of the SNP locus corresponds to a low-cold-resistance individual. The cold resistance prediction accuracy in the F1-generation hybrid population reaches 94.94%, and the prediction accuracy in the natural population reaches 96.15%. The molecular marker detection method disclosed by the invention is convenient and rapid, has good specificity and high accuracy, is not influenced by climate, environment and the like, can rapidly and effectively evaluate the cold resistance of natural resources and hybrid filial generations of the actinidia chinensis, and provides powerful technical support for breeding cold-resistant varieties of the actinidia chinensis.

The application discloses a kiwi bacterial canker disease susceptible gene AcADC1 and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the AcADC1 gene is shown in SEQ ID NO:1, and the arginine decarboxylase (ADC) synthesized by the gene is encoded, and the amino acid sequence is shown in SEQ ID NO:2, and the arginine (Arg) is responsible for catalyzing the formation of putrescine. The resistance of the kiwi plant with the silenced AcADC1 gene to the bacterial canker disease is improved, the resistance of the kiwi plant with the overexpressed AcADC1 gene to the bacterial canker disease is reduced, the AcADC1 gene plays a negative regulation role in the immune response of the kiwi to the bacterial canker disease, the gene editing technology can be used for editing the AcADC1 gene, and a theoretical basis and gene reserve are provided for kiwi disease-resistant breeding.

The application provides a method for efficiently breeding Actinidia chinensis, comprising the following steps: taking leaves or stem segments of Actinidia chinensis as explants, and performing culture treatment including induction culture on the explants to obtain Actinidia seedlings; wherein an induction culture medium used in the induction culture comprises an MS culture medium and an additive, the additive is selected from at least two of KT, 2,4-D, 6-BA, NAA, IBA and IAA; and in the culture medium, the content of the KT is 0.05-0.1 mg / L, the content of the 2,4-D is 0.5-1.0 mg / L, the content of the 6-BA is 0.1-0.5 mg / L, the content of the NAA is 0.5-1.0 mg / L, the content of the IBA is 0.01-0.05 mg / L, and the content of the IAA is 0.5-1.0 mg / L. The method provided by the application overcomes the influence of environmental factors on the breeding of Actinidia chinensis, and helps the protection of biodiversity.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with the germination rate of actinidia chinensis and a primer. The SNP molecular markers disclosed by the invention are Chr1518329400 and Chr1518648395 which are both positioned on the No.15 chromosome of the actinidia chinensis, are tightly associated with the spring branch germination rate (GR) character of the actinidia chinensis, and can be used for quickly and effectively evaluating the germination condition of natural populations and hybrid offspring of the actinidia chinensis after low-temperature freezing injury in winter. The cold resistance detection accuracy rates of the SNP molecular marker in an F1-generation hybrid population of actinidia chinensis are 69.23% and 89.01% respectively, and the cold resistance detection accuracy rates of the SNP molecular marker in a natural population are 80.77% and 92.31% respectively. The molecular marker detection method disclosed by the invention is convenient and rapid, good in specificity and high in accuracy, is not influenced by climate, environment and the like, can be used for rapidly and effectively predicting and evaluating the germination rate of natural resources and hybrid filial generations of the actinidia chinensis after freezing injury, provides powerful technical support for breeding cold-resistant varieties of the actinidia chinensis, and has a relatively high application value.

The present application relates to the technical field of plant molecular biology, and proposes a kiwi drought-resistant gene AcNFXL1 and application thereof.A kiwi drought-resistant gene AcNFXL1 The nucleotide sequence of the kiwi drought-resistant gene AcNFXL1 is shown as SEQ ID NO.1.Through the above technical solution, the problem of poor drought resistance of kiwi in the prior art is solved.

The invention relates to the technical field of traditional Chinese medicine pharmacy, and particularly discloses a cough and asthma patch and a preparation method thereof. The cough and asthma patch is prepared from active ingredients and a matrix, the active ingredients are prepared from folium eriobotryae, almonds, elaeagnus pungens, actinidia chinensis roots, thyme, polygala japonica, elephantopus scaber, gynura bicolor, veronica didyma, borneol and mentholum, and the matrix is bionic polymeric gel. The preparation method comprises the following steps: S1, preparing the extract; s2, colloid melting: melting the bionic polymer gel; s3, mixing to prepare paste: adding the extract obtained in S1, borneol and mentholum into the molten colloid obtained in S2, and stirring and mixing; and S4, forming. The cough and asthma patch can be used for relieving cough and asthma symptoms caused by asthma and chronic bronchitis, and has the advantages of being rapid in effect taking and lasting in medicine effect; in addition, the preparation method of the cough and asthma patch has the advantages of improving the transfer rate of the active ingredients, the homogeneity of the paste and the low-temperature stability.

The invention provides a gene AcANR1 for regulating and controlling the accumulation of proanthocyanidins in kiwi fruits. The nucleotide sequence of the gene AcANR1 is shown as SEQ. ID. NO. 1. The invention also provides a recombinant expression vector containing the gene, a recombinant agrobacterium and application of the gene in improvement of plant procyanidine accumulation and cultivation of high-quality kiwi fruit. The proanthocyanidins anabolism regulation gene AcANR1 is screened from kiwi fruits by combining kiwi fruit genome and transcriptome database expression analysis, and the accumulation amount of proanthocyanidins in plants can be remarkably increased through overexpression of the gene, so that the proanthocyanidins anabolism regulation gene AcANR1 is obtained. The method has important theoretical significance and practical application value on analysis of a kiwi fruit procyanidine synthesis regulation molecular mechanism, breeding of high-quality kiwi fruit varieties and kiwi fruit quality improvement.

The invention discloses a plant growth regulator for improving crop stress resistance and a preparation method and application thereof, and belongs to the technical field of plant growth regulators. The plant growth regulator disclosed by the invention is prepared from the following raw materials in parts by weight: 10 to 20 parts of betaine, 15 to 25 parts of fulvic acid, 80 to 100 parts of monopotassium phosphate, 3 to 8 parts of amino-oligosaccharin, 50 to 70 parts of compound microorganism fermentation liquor, 5 to 15 parts of radix actinidiae chinensis extract and 160 to 200 parts of water, wherein the compound microorganism fermentation liquor comprises bacillus cereus and brevibacterium paragasteroides. According to the plant growth regulator disclosed by the invention, the Chinese actinidia root extract and the compound microorganism fermentation liquor are innovatively added, so that the stress resistance of the economic crop pepper is remarkably enhanced, the antioxidant enzyme activity of pepper seedlings can be improved, osmotic regulation substances can be increased, and MDA accumulation in bodies is reduced, so that the tolerance of plants to high temperature and other adverse situations is greatly improved; the stability of plants in a severe environment is higher, and the influence of high temperature stress in summer on pepper seedling culture in summer is relieved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an AcBBX19 gene and application thereof in regulation and control of fruit ester aroma. The invention provides a kiwi fruit AcBBX19 gene related to ester aroma formation and a protein coded by the kiwi fruit AcBBX19 gene, and constructs a corresponding overexpression and silence vector to be transferred into kiwi fruits. Results show that the content of ester aroma in kiwi fruits can be reduced and increased by respectively overexpressing and silencing the AcBBX19 gene. The actinidia chinensis AcBBX19 gene disclosed by the invention can be used for regulating and controlling the formation of ester aroma. The method disclosed by the invention has very important application value in the direction of accurately creating the new germplasm of the storage-resistant kiwi fruit through a genetic improvement mode.

The present invention belongs to the technical fields of molecular biology and genetic breeding, and discloses 21 molecular marker primer combinations for the identification of Actinidia deliciosa and their applications. Using the 21 pairs of primers provided by the present invention to amplify the DNA of 16 common Actinidia deliciosa, bands of different sizes can be specifically amplified; according to the sizes and numbers of different bands, Actinidia deliciosa such as 'Jade', 'Morning Light', 'Cuixiang', 'Guichang', 'Huamei', 'Hayward', 'Jinfu', 'Jinkui', 'Miliang', 'Qinmei', 'Qiuming', 'Ruiyu', 'Tianyu', 'Xuxiang', 'Yate', 'Yuxiang', etc. can be distinguished.

This invention discloses a *Rummeliibacillus* sp. and its application, relating to the field of microbial technology. The *Rummeliibacillus* sp., named *Rummeliibacillus* JY-2-4R, was deposited on February 11, 2026, at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession number GDMCC No: 67853. This invention applies the strain to the preparation of preparations antagonistic to *Actinidia chinensis* black spot, *Actinidia chinensis* soft rot, *Actinidia arvensis* white rot, *Actinidia arvensis* soft rot, or *Actinidia arvensis* root rot. The *Rummeliibacillus* JY-2-4R of this invention is a potential biological agent possessing stress resistance, growth promotion, and broad-spectrum antibacterial activity, with broad development potential and good application prospects, addressing the problem of a lack of functional microorganisms for the biological control of kiwifruit and *Actinidia arvensis* diseases.

The present application relates to a tobacco product, a plant spice and the use of the plant spice in the preparation of the tobacco product. The tobacco product comprises the plant spice, wherein the plant spice is prepared by means of mixing a plant raw material with a deep eutectic solvent for extraction. The plant raw material comprises one or more of Rosa roxburghii, Ficus carica, Tamarindus indica, Hippophae rhamnoides, Malus domestica, Glycyrrhiza uralensis, Siraitia grosvenorii, Fructus mume, Passiflora edulis, Actinidia chinensis and Crataegus pinnatifida.

The invention relates to the field of plant germplasm resource preservation, in particular to a kiwi fruit stem tip vitrification ultralow-temperature preservation method which comprises the following steps: S1, pretreatment: taking stem tips at terminal buds after kiwi fruit seedling culture, and placing the stem tips in a pre-culture solution for shake culture; s2, loading a cryoprotectant: transferring the stem tip into a sterile cryopreservation tube pretreated at 4 DEG C, placing the stem tip and the sterile cryopreservation tube in an environment of 0 DEG C, and adding the cryoprotectant into the tube; s3, performing dehydration treatment and cryopreservation; the stem tips are transferred into a PVS2 solution and placed in an environment at the temperature of 0 DEG C to be subjected to dehydration treatment for 40 min; dropping a drop of PVS2 solution on the sterilized aluminum foil strip, and wrapping the dehydrated stem tip in the dropping liquid of the PVS2 solution of the sterilized aluminum foil strip; transferring the aluminum foil strip to be subjected to stem tip into a freezing pipe pre-frozen by liquid nitrogen, putting the freezing pipe into a basket of a storage box, and then putting the basket into a liquid nitrogen storage tank for ultralow-temperature freezing storage. The method has the beneficial effect that the preservation effect of the Chinese kiwi fruit 'Hongyang' stem tips is improved.

The invention belongs to the technical field of plant tissue culture and rapid propagation, and particularly provides a method for tissue culture and rapid propagation of actinidia chinensis, which comprises the following steps: obtaining and pretreating an explant; performing ultra-clean inoculation; performing primary culture; carrying out subculture multiplication culture; and acclimatizing and transplanting. According to the method disclosed by the invention, the stem section of the chiliott is used for performing tissue culture to obtain a sterile seedling which grows well, and the explant is successfully induced to root during subculture multiplication culture, so that the propagation cycle of the chiliott tissue culture is greatly shortened. A tissue culture and rapid propagation system of the lizardtail fig is successfully constructed by adjusting stem tissue culture, a sterilization method, the variety and concentration of a plant growth regulator and seedling strengthening and rooting.

Disclosed are a traditional Chinese herbal plant composition for improving symptoms of liver cancer patients and a compatibility method thereof. The herbal plant composition consists of the following raw materials by weight: 20-28 parts of yangtao actinidia root (Actinidia chinensis Planch.), 17-22 parts of bird's-foot trefoil (Lotus corniculatus L.), 12-15 parts of shorthairy antenoron (Anoectochilus roxburghii (Wall.) Lindl.), 7-10 parts of giant knotweed rhizome (Polygoni Cuspidati Rhizoma et Radix), 1-5 parts of fruit of mountain spicy tree (Piper cubeba L.f.), 5-10 parts of astragalus milkvetch root (Astragali Radix), 1-5 parts of hispid fig root (Ficus simplicissima Lour.), and 1-3 parts of liquorice root (Glycyrrhizae Radix et Rhizoma). The herbal plant composition can inhibit the growth of liver cancer cells without side effects.

This invention discloses a traditional Chinese medicine composition for dissolving nodules and tumors, its preparation method, and its application. The composition comprises the following components in parts by weight: 4-8g of *Taxus wallichiana*, 20-40g of *Actinidia chinensis* root, 5-25g of *Lobelia chinensis*, 20-40g of *Scutellaria barbata*, 20-40g of *Hedyotis diffusa*, 1-5g of *Scolopendra subspinipes*, 5-15g of *Curcuma zedoaria* (processed with vinegar), 5-25g of *Solanum lyratum*, 20-40g of *Astragalus membranaceus*, 5-15g of *Angelica sinensis*, 5-25g of *Pseudostellaria heterophylla*, 5-25g of *Poria cocos*, 5-25g of *Atractylodes macrocephala* (stir-fried with wheat bran), 20-40g of *Coix lacryma-jobi*, 5-15g of *Pinellia ternata* (processed with ginger), 5-15g of *Citrus reticulata* peel, and 5-15g of *Glycyrrhiza uralensis*. This formula combines tonification and purgation, nourishing qi and blood, regulating the spleen and stomach, and dissolving nodules and tumors. This invention, through the addition and subtraction of prescription drugs, can dissolve nodules and eliminate tumors in the early stage of gastric and intestinal tumor treatment, while protecting the spleen and stomach's qi and blood; in the middle stage of gastric and intestinal tumor treatment, it can strengthen the spleen, resolve dampness, detoxify and disperse nodules, combining attack and tonification; in the terminal stage of gastric and intestinal tumor treatment, it can invigorate qi and support the body's resistance, while also having anti-cancer effects.

This invention belongs to the field of traditional Chinese medicine quality testing technology, specifically disclosing a method for constructing a characteristic chromatogram and determining the content of *Actinidia chinensis* root. The method for constructing the characteristic chromatogram includes the following steps: S1: Take *Actinidia chinensis* root as a reference material, add 70% methanol for ultrasonic extraction, filter, and obtain a reference solution; separately take catechin, epicatechin, proanthocyanidin B1, and proanthocyanidin B2 reference standards, add 70% methanol to prepare a mixed reference solution; S2: Take *Actinidia chinensis* root powder, add 70% methanol for ultrasonic extraction, add weight, filter, and obtain the final product; S3: Inject the samples separately, record the chromatograms, and construct the characteristic chromatogram. This invention establishes a characteristic chromatogram of *Actinidia chinensis* root using high-performance liquid chromatography (HPLC) and simultaneously determines the content of four components: proanthocyanidin B1, catechin, proanthocyanidin B2, and epicatechin, achieving comprehensive, accurate, and stable quality control of *Actinidia chinensis* root.