170 results about "Actinidia chinensis" patented technology

The invention discloses a cultivation method for the root stock grafting of Actinidia chinensis Planch, comprising the grafting and cultivating steps of cultivating parental stock and selecting a shoot bud that is used for producing Actinidia chinensis Planch as an ear bud. The invention is characterized in that in the cultivation step of the parental stock, the selected root stock material is Actinidia polygama (alias: sky-high polygonum; plant name: silvervine; Latin name: Actinidia polygama). The Actinidia polygama is a wild plantlet which is collected from the investigation of a deep ravine everglade with the elevation of 1500 meters, has a highly-developed fiber root system, shows strong appetency to the grafting of Actinidia chinensis Planch and is a completely new parental stock breed. Compared with the prior art, the invention has the advantages of strong resistance abilities of cold, drought, saturation and canker, low soil requirements, good adaptability, convenient cultivation and management, simple soil displacement, low investment and low cost, etc.; furthermore, the invention can implement asexual propagation and is favorable for the sustainable and protective utilization of wide plant resources.

The present invention provides a preparation method of a health-care blueberry beverage, and includes the following raw materials: blueberries, herba dendrobii, ginger, schisandra chinensis, sugar stevia leaves, Chinese wolfberries, guavas, carrots, sapodilla, grapes, actinidia chinensis, haws, bitter apricot kernels, corn, oats, soybeans and green tea. The beverage contains a variety of effective ingredients and nutritional ingredients including polysaccharides, flavonoids, flavonoids, saponins, peptides, vitamins, etc. The beverage can enhance and regulate immune function of human body, protect liver and improve eyesight, regulate intestinal microflora, resist aging, protect skin and beautify appearance, eliminate fatigue, prevent cardiovascular and cerebrovascular diseases, etc.

The invention discloses a kiwi fruit InDel molecular marker and a screening method and application thereof. Two different cold-proof Actinidia arguta genome DNAs are used as research subjects, resequencing of four variety genes is carried out, InDel primers are designed according to resequencing data, and PCR (polymerase chain reaction) amplification, agarose electrophoresis and non-denaturing polyacrylamide gel electrophoresis are carried out; polymorphism detection is carried in Actinidia arguta to screen out 14 pairs of InDel primers, the 14 pairs of InDel primers are applicable to the genetic diversity analysis for Actinidia chinensis, Actinidia deliciosa and the like and are also applicable to the researches, such as hybrid offspring authenticity identification, genetic map construction, and molecule-assisted breeding. The kiwi fruit InDel primers according to the embodiment of the invention has good mutation stability and low detection difficulty, allow InDel insertion/loss of large fragments, and allows agarose analysis, with steps that may be simplified.

The invention discloses a tissue culture and rapid propagation method for actinidia chinensis, and relates to the tissue culture and rapid propagation method for actinidia chinensis seedlings. According to the method, young leaves of the current year serve as explants and are inoculated on a medium MS+0.3mg/LZT+0.05mg/LNAA subjected to callus induction and cluster bud differentiation. The invention also provides an improved MS; after cluster buds are differentiated, the cluster buds are sub-cultured in an improved MS+2mg/LZT proliferation culture medium, and are transferred into an improved MS+0.015mg/LTDZ thickening culture medium according to seedling height; small seedlings which are in accordance with rooting are transferred into 1/2 of improved MS+0.7mg/LIBA+0.1g/LAC rooting culture medium; the small seedlings are transplanted and are domesticated after roots grow. By the method, the callus induction rate reaches 96.4 percent; the height of cluster bud reaches 1.67cm; the proliferation coefficient of cluster buds reaches 3.67; the rooting rate reaches 98.12 percent; moreover, the culture medium is low in cost and short in propagation period, and industrial production can be realized.

The invention provides a cultivation method for actinidia chinensis. The method includes the following steps of (1) orchard selection, wherein a region which has good ventilation and light transmitting, is located on the lee side, is exposed to the sun, gets close to a water source and has high humidity is selected as an orchard; (2) soil amelioration and preparation, wherein ditching is conducted twice through a deep harrowing machine first, and farmyard manure is applied; (3) planting, wherein planting points are fixed with lime according to row spacing of 6 m and line spacing of 2 m, small planting pits which are 20 cm deep and 30 cm wide are dug in the planting points, seedling roots are naturally arranged during planting, the roots are buried with damp fine soil, the fine soil is treaded down with feet, root fixing water is sprayed sufficiently, and moisture maintenance is conducted through mulching film covering or weed covering; (4) cultivation and management, wherein fertilization and hilling are carried out according to growth situations of seedlings of actinidia chinensis, and management of flowers and fruits is conducted. Cultivation standardization of actinidia chinensis is achieved through the cultivation method, the yield of actinidia chinensis cultivated through the cultivation method is high, no pesticide or chemical residue is generated, and the mineral content is high.

The invention discloses a real-time fluorescence PCR method for quantitatively detecting Pseudomonas syringae pv. Actinidiae. The method comprises the following steps: extracting an Actinidia chinensis sample genome DNA; electrophoretically detecting the band size and purity of the Actinidia chinensis sample genome DNA; carrying out fluorescent quantitative detection on a PSA pathogen; carrying out specific primer-based fluorescent quantitative amplification, and preliminarily determining whether the PSA pathogen is contained or not according to an amplification curve; purifying a PCR product with an obvious amplification peak, and sequencing the purified PCR product; and comparing a sequence obtained after the sequencing by Internet to further determine the PSA pathogen. The method allows a fluorescent quantitative PCR-based rapid detection method of the pathogen to be established, realizes rapid identification of the Pseudomonas syringae pv. Actinidiae infected sample, has the characteristics of simplicity in operation, high sensitivity, high specificity, and accurate and objective detection result, and is of great significance to improve the detection efficiency and the detection sensitivity of the Pseudomonas syringae pv. Actinidiae infected sample.

The invention discloses an actinidia chinensis planch-sandwiched fruit sugar and a preparation method thereof, belonging to the technical field of food processing. The preparation method comprises the following steps: 1) removing the peel of the actinidia chinensis planch, and cutting the actinidia chinensis planch into fruit slices; 2) carrying out ultra high pressure treatment on the actinidia chinensis planch fruit slices, and then, soaking the fruit slices into a penetrating fluid for osmotic treatment; 3) washing the actinidia chinensis planch fruit slices subjected to osmotic treatment, then, carrying out vacuum low-temperature drying, and thus obtaining an actinidia chinensis planch core material; 4) immersing the actinidia chinensis planch core material in sugar liquid, taking out the actinidia chinensis planch core material and spreading the sugar liquid evenly while being hot, then, immersing the actinidia chinensis planch core material in sugar liquid again, taking out, and spreading the sugar liquid evenly, repeating the immersing and spreading operations until forming a shell sugar body in needed specifications, cooling and drying, and thereby obtaining the Actinidia chinensis planch-sandwiched fruit sugar. The method is simple to operate and suitable for large-scale production; the actinidia chinensis planch-sandwiched fruit sugar prepared through the method keeps the appearance color and nutrition quality of the actinidia chinensis planch better, and is fresh and delicious.

The invention relates to an anti-blue-light cushion CC (color correcting) cream and a preparation method thereof. The anti-blue-light cushion CC cream comprises, by weight, 1-5 parts of an emulsifier,15-35 parts of a skin moisturizer, 20-35 parts of an ultraviolet absorbent, 5-15 parts of a moisturizing component, 0.1-2 parts of a film-forming agent, 2.0-10.0 parts of Durvillea antartica extract,and 1.0-5.0 parts of Actinidia chinensis extract; preferably, the weight ratio of the Durvillea antartica extract to the Actinidia chinensis extract is (0.1-10):1. The anti-blue-light cushion CC cream can resolve skin damage due to ultraviolet, can resist skin damage due to blue light, and has a multi-effect restoring function. Furthermore, the anti-blue-light cushion CC cream also has excellentmoisturizing effect and can provide natural nude makeup.

The invention discloses a nutrient solution for grafting actinidia chinensis. The nutrient solution is prepared from the following components in parts by weight: 2 to 3 parts of juice calcium, 2 to 4 parts of auxin, 0.02 to 0.03 part of active polypeptide, 0.5 to 0.6 part of compound amino acid, 10 to 12 parts of nitrogen, phosphorus and potassium fertilizer, 30 to 40 parts of an organic fertilizer fermented solution, 0.10 to 0.12 part of calcium superphosphate, 0.1 to 0.2 part of a urea-Ni morphological compound, 0.10 to 0.15 part of potassium chloride, 0.15 to 0.18 part of ferrous sulfate, 0.05 to 0.08 part of copper sulfate, 0.05 to 0.08 part of zinc sulfate, 0.08 to 0.1 part of magnesium borate, 5 to 8 parts of plant ash and 900 to 950 parts of distilled water. The nutrient solution disclosed by the invention has the beneficial effects that the nutrient solution can be used for promoting the growth of cells, promoting sprouting and growth and nutrient absorption of plants and increasing the grafting survival rate of the actinidia chinensis, so that the nutrient solution for grafting the actinidia chinensis has abundant nutrients and is efficient and stable.

The invention relates to a polyploid induction method for Chinese gooseberry Hort 16A offspring fine strain tissue culture seedlings. The polyploid induction method comprises the following steps: soaking leaves of the Hort 16A offspring strain tissue culture seedlings in a colchicine water solution to induce polyploidy, washing the leaves, and inoculating in callus differentiation enrichment culture medium and subculture culture medium for light culture according to the steps. The polyploid induction method is simple in operation, good in induction directionality, high in induction success rate, low in cost, shorter in culture period and higher in induction rate, an efficient and feasible new method is provided for polyploidy breeding of Chinese gooseberry, the polyploid induction method has important application values, through the popularization of the polyploid breeding method, economic benefits can be brought for fruit farmers, and demands of more consumers are met.

The invention provides a tissue-culture seedling culture method of semi-lignified stems with buds of Hongyang actinidia chinensis. The method comprises four technical links including explant disinfection treatment, axillary bud primary induction culture, cluster bud subculture multiplication culture and rooting culture; the method takes the semi-lignified stems with the buds, which are sprouted for 15 to 20 days, as explants; controlling the browning pollution rate of the explants to be 10 percent or lower through a method of immersing and disinfecting through an antioxidant Vc and citric acidmixed distilled water solution and a potassium permanganate distilled water solution in sequence; finally, adsorbing harmful substances and inhibiting decomposition of phenol substances through adding activated carbon and polyvinylpyrrolidone in culture media of two phases including the primary induction culture and the subculture multiplication culture, so as to ensure that the induction differentiation rate of cluster buds is 95 percent or more and the multiplication coefficient is 9.0 or more. Peptone is added into a rooting culture medium, the rooting rate reaches 100 percent and 5 to 8 roots are differentiated from a root system of each plant. The method provided by the invention is simple and feasible, and provides evidences for integration of a industrialized cultivation technologysystem of improved-variety germchits of the actinidia chinensis.

The present disclosure encompasses compositions prepared from kiwifruit. In particular, the invention encompasses compositions prepared from gold varieties of Actinidia chinensis. Also encompassed are methods of preparing these compositions. Further encompassed are methods of using these compositions, in particular, for treating or preventing disorders of the gastrointestinal system, including amongst others: inflammation, constipation, bowel irregularity, microbiota imbalances, irritable bowel syndrome, and inflammatory bowel disease.

The invention provides a method for relieving drought stress of Actinidia chinensis fruit trees. The method comprises the following step of irrigating melatonin at the concentration of 50 to 200 umol/L in seedlings of the Actinidia chinensis for one time every other 2 days, totally for four times, wherein the irrigation amount is 200 ml. By the method, the relative water content of leaves of the seedlings of wild Actinidia chinensis in the same year under the drought stress can be obviously increased, damage of malonaldehyde to cytomembrane is reduced, the relative membrane permeability is reduced, proline concentration and soluble sugar concentration are reduced, degradation of soluble protein is inhibited, high activity of protective enzyme is maintained, the plants can adapt to the drought environment better and the drought resistance of the plants is enhanced.

The invention discloses a high yield cultivation method for Actinidia chinensis. The method is characterized in that: a special nutrient solution for Actinidia chinensis or a fruit tree nutrient solution is selected, the basal part of the trunk of a diseased tree is drilled to the medulla of the diseased tree, a stock solution of the nutrient solution is used according to a case that 1ml of the stock solution corresponds to the trunk with the diameter of 1cm, the nutrient solution is conveyed to the medulla, the stock solution is diluted 30 times when the stock solution is used after leaf falling and before dormancy, and the stock solution is diluted 50 times when the stock solution is used in the growth season. By adopting the special nutrient solution for Actinidia chinensis or the fruit tree nutrient solution and directly conveying the nutrient solution to the medulla of the Actinidia chinensis tree through carrying out an infusion process in the invention, the nutritional deficiency yellows of Actinidia chinensis can be rapidly, directly and effectively controlled, so the method has practical popularization and application values.

The invention relates to a preparation method of fruit vinegar fermented from Lucid Ganoderma. The preparation method comprises the following steps: 1, selecting 70% of Lucid Ganoderma (wall broken Lucid Ganoderma) and 30% of millet, fermenting by a traditional fermentation process, extracting a fermentation broth; 2, mixing 20% of a Pyrus serotina juice, 20% of an apple juice, 20% of a sea buckthorn juice, 20% of an Actinidia chinensis juice and 20% of a tomato juice to obtain a mixed juice; 3, taking 500kg of purified water, adding 4% of the fermentation broth, 4% of the mixed juice and uniformly stirring according to a preparation proportion of 92%; and 4, adding relevant accessories, uniformly mixing, sterilizing, canning, sealing, cooling, and packaging. So the fruit vinegar is obtained.

The invention discloses a stereoscopic planting method of actinidia chinensis planch-colocasia esculenta-zizania latifolia. The method comprises the steps that 1) ditching and banking are carried outon the periphery of a planting base, and a surrounding ditch in a dam is connected with a ditch system in an actinidia chinensis planch orchard to form a closed circulation environment capable of naturally collecting water; 2) after mechanical operation is carried out to centralize the surface soil in the planting base, an actinidia chinensis planch planting ridge is formed each 8 meters, two lines of actinidia chinensis planches are planted on one ridge, and 90-100 plants of actinidia chinensis planches are planted per mu; 3) a gutter with 1-1.2 meters of width is dug between actinidia chinensis planch planting ridges, the dug soil heightens the soil at the two sides, and the colocasia esculenta is planted at the two sides; and the zizania latifolia is planted in the gutter; 4) geese arestocked in a plantation, ecological species of misgurnus anguillicaudatus and fogs are bred in the gutter, and the natural purification of water quality is ensured. The stereoscopic planting method ofthe actinidia chinensis planch-colocasia esculenta-zizania latifolia has the advantages that the high-quality production of the actinidia chinensis planch, colocasia esculenta, zizania latifolia is promoted, and the planting method is a new technical mode of protecting the environment and promoting sustained and efficient development of argriculture.

The invention discloses an efficient and comprehensive actinidia chinensis transplantation treatment agent. Each gram of the treatment agent comprises: 0.005g-0.01g of a bactericide; 0.1g-1g of an insecticide; 0.2g-0.5g of ethanol; 1*10<-4>g-2*10<-4>g of gibberellin; 3*10<-7>g-5*10<-7>g of indoleacetic acid; and 1*10<-7>g-4*10<-7>g of brassinolide. The treatment agent provided by the invention contains gibberellins, brassinolide and indoleacetic acid, which can break the period of dormancy of actinidia chinensis roots to accelerate germination and improve the survival rate of transplantation. The treatment agent contains ethanol, which can promote the efficacy of gibberellins, brassinolide and indoleacetic acid in breaking the period of dormancy of actinidia chinensis, thus having a strengthening effect.

A preparation method of grass carp-fishing bait comprises spinach powder, celery powder, swamp cabbage powder, Chinese cabbage powder, carrot powder, pumpkin powder, white gourd powder, apple powder, banana powder, pineapple powder, muskmelon powder, actinidia chinensis powder, sweet potato powder, shrimp powder, and corn powder, wherein the percentage is weight percentage; the bait formula and the preparation method are provided; the preparation method is simple; the bait has low price, thick fragrance, sweet taste, and good fish-baiting effect, and overcomes the defects of absence of fragrance, small grass carp-fishing amount, slow fishhook swallowing by grass carp, and poor grass carp-fishing/catching effect of traditional grass carp bait.

The invention discloses a rootstock grafting cultivation method for actinidia chinensis planch. The method comprises the following steps: cultivating rootstocks, and selecting branch buds of production actinidia chinensis planch trees as preharvest sprouts for grafting cultivation. The method is characterized in that actinidia polygama (alias: Tianmuliao, plant name: Gezao Mihoutao, Latin name: actinidia polygama) is selected as a rootstock material in the step of cultivating the rootstocks. The actinidia polygama is a wild plant surveyed and collected in a remote mountains and wetlands, has a well-developed fiber root system, is high in affinity when being grafted with the actinidia chinensis planch, and is a novel rootstock variety. Compared with the prior art, the method has the advantages of high cold resistance, drought resistance, stain resistance and canker resistance, no requirements on soil, high adaptability, convenience for cultivation management, simplicity for soil improvement, low investment, low cost and the like. In addition, asexual reproduction can be implemented, and the method is favorable for sustainably protecting and utilizing wild plant resources.

The invention provides a bactericide for gray mold of actinidia chinensis. The bactericide is prepared from the following components by weight: 400-600g of deionized water, 4-6g of emulsifier, 15-25g of chloroisobromine cyanuric acid, 2-4g of cyrtomium rhizome extract, 2-4g of perilla extract, 2-4g of cypress bark extract, 2-4g of derris trifoliata extract, 4-6g of matrine, 4-6g of amoxicillin, 3-5g of clavulanic acid, 2-4g of tigecycline, 4-6g of alpha-cypermethrin, 3-5g of compound sodium nitrophenolate, 2-4g of benzoic acid, 2-4g of copper sulfate, 2-4g of tobramycin, 2-4g of anthocyanin from purple sweet potatoes and 2-4g of ethylparaben. The bactericide is prepared by the steps of heating the deionized water to 50-65 DEG C, putting the rest components into the deionized water, conducting continuous stirring for about 10 minutes, and conducting cooling to normal temperature to obtain the bactericide. By adopting the bactericide, gray mold of actinidia chinensis can be effectively prevented and controlled, and germs can be killed. The bactericide cannot be remained in a plant body under a normal application technical condition, and has small influence on the natural environment and other organisms.

The invention discloses foliar bacterial fertilizer for actinidia chinensis as well as a preparation method and application thereof. The foliar bacterial fertilizer is prepared from 20 to 40 parts ofbacillus cereus bacterium agent, 8 to 12 parts of chitosan, 8 to 12 parts of amino acid, 10 to 20 parts of micro-fertilizer and 20 to 40 parts of water. The foliar bacterial fertilizer is used for increasing the yield of the actinidia chinensis, improving the quality of the actinidia chinensis, improving the storage performance of the actinidia chinensis, inducing to enhance the disease resistanceof the actinidia chinensis, promoting the growth of low-age and forested actinidia chinensis plants and the like. The foliar bacterial fertilizer for the actinidia chinensis, disclosed by the invention has the advantages of remarkable effect, greenness and no pollution, high fertilizer utilization ratio, disease prevention and control, improvement of yield and quality, low cost, easiness for popularization and the like.