Tissue-culture seedling culture method of semi-lignified stems with buds of Hongyang actinidia chinensis

A semi-lignified, kiwi fruit technology, applied in the field of "Hongyang" kiwi fruit with semi-lignified buds and stems in the field of group cultivation, to achieve the effect of simple and easy method, and improve the ability of anti-browning

Active Publication Date: 2018-09-14
贵州省山地资源研究所
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  • Abstract
  • Description
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  • Application Information

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Embodiment 1

[0020] (1) Disinfection treatment of explants: In April 2017, 30 semi-lignified shoots of "Hongyang" kiwi fruit that had just germinated for 15 days were collected, rinsed with tap water and distilled water 5 times, and left with 2 to 3 bud stems after removing the leaves section; use 3000mg.L -1 Vitamin C and 2000mg.L -1 Soak citric acid mixed with distilled aqueous solution for 50min, rinse with distilled water 5 times; use 4500mg.L -1 Soak in potassium permanganate distilled water solution for 40min, rinse with distilled water 5 times; cut the bud stem section into 1.5cm single bud stem section, and then use 1000 mg.L -1 Mercury chloride aqueous solution disinfection 15min, distilled water rinse 5 times;

[0021] (2) The basic medium for each stage of plant cell tissue culture is: 1 / 2MS+12 g.L -1 Agar+35 g.L -1 Sucrose, pH 6.0; culture conditions at each stage: temperature 24°C-26°C, light 1500-2000Lux, light time 12-14h / d;

[0022] (3) Primary induction culture of axi...

Embodiment 2

[0026] (1) Disinfection treatment of explants: In April 2017, 30 semi-lignified shoots of "Hongyang" kiwi fruit that had just germinated for 20 days were collected, rinsed with tap water and distilled water 5 times, and left with 2 to 3 buds after removing the leaves Stem section; use 3000mg.L -1 Vitamin C and 2000mg.L -1 Soak citric acid mixed with distilled aqueous solution for 50min, rinse with distilled water 5 times; use 4500mg.L -1 Soak in potassium permanganate distilled aqueous solution for 40 minutes, rinse with distilled water 5 times; cut the stem with buds into single buds with a length of 1.5cm, and then use 1000 mg.L -1 Mercury chloride aqueous solution disinfection 15min, distilled water rinse 5 times;

[0027] (2) The basic medium for each stage of plant cell tissue culture is: 1 / 2MS+12 g.L -1 Agar+35 g.L -1 Sucrose, pH 6.0; culture conditions at each stage: temperature 24°C-26°C, light 1500-2000Lux, light time 12-14h / d.

[0028] (3) Primary induction cult...

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Abstract

The invention provides a tissue-culture seedling culture method of semi-lignified stems with buds of Hongyang actinidia chinensis. The method comprises four technical links including explant disinfection treatment, axillary bud primary induction culture, cluster bud subculture multiplication culture and rooting culture; the method takes the semi-lignified stems with the buds, which are sprouted for 15 to 20 days, as explants; controlling the browning pollution rate of the explants to be 10 percent or lower through a method of immersing and disinfecting through an antioxidant Vc and citric acidmixed distilled water solution and a potassium permanganate distilled water solution in sequence; finally, adsorbing harmful substances and inhibiting decomposition of phenol substances through adding activated carbon and polyvinylpyrrolidone in culture media of two phases including the primary induction culture and the subculture multiplication culture, so as to ensure that the induction differentiation rate of cluster buds is 95 percent or more and the multiplication coefficient is 9.0 or more. Peptone is added into a rooting culture medium, the rooting rate reaches 100 percent and 5 to 8 roots are differentiated from a root system of each plant. The method provided by the invention is simple and feasible, and provides evidences for integration of a industrialized cultivation technologysystem of improved-variety germchits of the actinidia chinensis.

Description

technical field [0001] The invention relates to plant regeneration through tissue culture technology, and furthermore, relates to a method for group seedling cultivation of "Hongyang" kiwifruit semi-lignified stem segments with buds. Background technique [0002] Kiwifruit is a deciduous vine fruit tree of the Actinidiaceae Actinidia genus. Its fruit is rich in nutrients, and its vitamin content ranks first among all kinds of fruits. "Hongyang" kiwifruit is an excellent line selected from the seedlings of Chinese kiwifruit by the Agricultural Bureau of Cangxi County, Sichuan Province in 1989. Because of its fresh fruit cross section, there are purple-red lines along the fruit center, which are distributed radially, like the sun shining everywhere. The fruit of this variety is short and cylindrical, with fresh and tender flesh, a small core, strong fruit flavor, and excellent quality. The total sugar content is 13.45%, which is almost higher than that of the world's popular v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 张丽敏席培宇彭熙李安定蔡国俊张建利龙秀琴王济红
Owner 贵州省山地资源研究所
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