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A method for group-cultivating seedlings of "Hongyang" kiwifruit with semi-lignified bud stems
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A semi-lignified and kiwifruit technology, which is applied in the field of group cultivation of "Hongyang" kiwifruit semi-lignified and bud stem sections, achieves the effect of being simple and easy to implement and improving the ability of anti-browning
Active Publication Date: 2021-08-27
贵州省山地资源研究所
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Embodiment 1
[0018] (1) Owalgot disinfection treatment: April 2017, "Hongyang" kiwi semi-wood, 30, collected, was collected, and then rinsed with tap water, and the distilled water was flushed 5 times, and the leaves left to leave 2 ~ 3 spark stalks. Segment; use 3000 mg · L -1 Vitamin C and 2000mg · L -1 Citric acid mixed distilled aqueous solution for 50 min, the distilled water was rinsed 5 times; on the ultra-net workbench, 4500 mg · L -1 The potassium permanganate is soaked for 40 min, and the distilled water is rinsed 5 times; the strand stem segment is cut into length 1.5 cm single sprout stem segment, and then use 1000 mg · L -1 The mercury chloride is disinfected for 15 min, and the distilled water is flushed 5 times;
[0019] (2) The basic medium of plant cell tissue culture is: 1 / 2 ms + 12g · L -1 Joose +35 g · L -1 Sucrose, pH 6.0; each stage culture conditions are: temperature 24 ° C ~ 26 ° C, lighting is 1500 ~ 2000lux, the light time is 12 to 14 h / d;
[0020] (3) A primary ind...
Embodiment 2
[0024] (1) In April 2017, the "Hongyang" kiwi semi-woodcaoned new ships were collected for 20 days. After rinsing, the distilled water was flushed 5 times, and the leaves left the leaves 2 ~ 3 buds. Stem section; 3000 mg · L -1 Vitamin C and 2000 mg · L -1 Citric acid mixed distilled aqueous solution for 50 min, distilled water flush 5 times; on the ultra-net workbench, use 4500
[0025] Mg · L -1 The potassium permanganate is soaked for 40 min, and the distilled water is flushed 5 times; after cutting the buds of the sprouts into a single sprout segment of the length of 1.5 cm, use 1000 mg · l -1 The mercury chloride is disinfected for 15 min, and the distilled water is flushed 5 times;
[0026] (2) The basic medium of plant cell tissue culture is: 1 / 2 ms + 12g · L -1 Joose +35 g · L -1 Sucrose, pH 6.0; each stage culture conditions are: temperature 24 ° C ~ 26 ° C, lighting is 1500 ~ 2000lux, the light time is 12 to 14 h / d;
[0027] (3) A primary induction culture of the mutua...
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Abstract
A method for the group cultivation of "Hongyang" kiwi fruit with semi-lignified buds and stems. The method includes four technical links: explant disinfection treatment, initial axillary bud induction culture, clustered bud subculture proliferation culture and rooting culture. The method is as follows: The semi-lignified bud stem section that has just germinated for 15 to 20 days is an explant, and the browning pollution rate of the explant is sequentially soaked in distilled aqueous solution mixed with antioxidant Vc and citric acid, distilled aqueous solution of potassium permanganate, and disinfected. Control it below 10%; Finally, add activated carbon and polyvinylpyrrolidone to the medium of the initial induction and the second stage of subculture to absorb harmful substances and inhibit the decomposition of phenolic substances, so as to ensure that the differentiation rate of clustered buds is above 95%. The value-added coefficient is above 9.0. Add peptone to the rooting medium, and the rooting rate reaches 100%, with an average of 5-8 differentiated roots per plant. The method is simple and easy to implement, and provides a basis for the technical system integration of the industrial cultivation technology of the fine seedlings of kiwifruit.
Description
Technical field [0001] The present invention relates to plant regeneration through tissue culture techniques, and further, involving "red yang" kiwi semi-woodcained strains cultivation method. Background technique [0002] Kiwi is a kiwifruit, kiwi, a vitrified fruit tree, and its fruit is rich in nutrients. Its vitamin content is the first of a variety of fruits; the kiwifruit can be used in medicine, the roots, fruit and other parts of the extract have anti-tumor and oxidation. "Hongyang" kiwifruit is a superiority of the Sichuan Cangxi County Agricultural Bureau from the China Kiwi County Agricultural Bureau, due to its fresh fruit cross section, there is a purple red line, which is radialized, like the sun. Shoot, so "Red Yang Kiwi", the variety of fruit is short, the meat is tender, the fruit is small, the fruit is strong, the quality is excellent, the total sugar content is 13.45%, the total glue variety "Hayward" 5%, has become a variety of varieties in domestic planting i...
Claims
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