A kind of group cultivation method of purple fruit passionflower top shoot

Abstract

A method for group seedling cultivation of the apex of Passiflora violet, the method comprising anti-browning of explants, disinfection treatment, primary induction culture of axillary buds, induction culture of callus, subculture proliferation culture, rooting induction culture and bottle seedlings Transplanting technology link: take the young top shoots as explants, soak them in a mixed solution of antioxidant Vc and citric acid, and then sterilize them with potassium permanganate solution to improve the antioxidant and anti-browning effects of young explants. Ability and vitality; add activated charcoal and polyvinylpyrrolidone to the 3-stage medium of axillary bud induction, callus induction and subculture to absorb and secrete harmful substances and inhibit the production of browning substances. By adopting this method, the polluting rate and the browning rate of the initial induction of passionflower top shoots can be controlled below 10%; the initial induction survival rate of top shoots reaches more than 90%, the subculture multiplication coefficient of clustered buds is more than 5.0, and the rooting rate is more than 90%. ; The survival rate of transplanted bottle seedlings is over 95.0%. The invention can meet the needs of industrialized seedling cultivation.

Description

technical field [0001] The invention relates to crop cultivation techniques, in particular to a method for group seedling cultivation of purple passion fruit top shoots. Background technique [0002] Passiflora (scientific name: Passiflora caerulea L.), a perennial evergreen climbing woody vine, is a fragrant and delicious fruit and has the reputation of "king of juice". Its fruit is rich in calcium, iron, phosphorus and other trace elements and 17 kinds of amino acids and other substances. In addition to eating, it also has good health care and medicinal effects. According to the determination, passion fruit fruit contains more than 135 kinds of aromatic substances, which are most suitable for processing into fruit juice. But the planting passion fruit disease is more serious, mainly contains root rot, phytophthora, anthracnose and mosaic disease etc., especially in the perennial orchard, the disease is more serious, causes dropped fruit and defective fruit to increase, an...

AI Technical Summary

Problems solved by technology

Existing detoxification methods for passionflower seedlings all have complex operating procedures, explants are all derived from mature organs or tissues that may carry viruses and bacteria, and the detoxification effect of single shoot tip heat treatment is unstable, etc.

The newly germinated top shoots of passionflower belong to young tissues with strong differentiation ability, almost no viruses and germs, and are easy to brown after disinfection treatment. At present, the young top shoots of passionflower are used as explants, and cell division is adopted. The combination of TDZ (thiadizuron) and auxin NAA to induce axillary bud differentiation, callus differentiation and clustered bud differentiation has not been reported.