353 results about "Contamination rate" patented technology

The invention discloses a method for cultivating phellinus igniarius. The method includes the steps that firstly, short-cut wood is directly placed in a material bag or placed in the material bag after being bundled, the two end faces of the short-cut wood or the two ends of a short-cut wood bundle are paved with cultivation media, an opening of the bag is tightened, and then a material package is acquired; secondly, the material package is sterilized, cooled and then inoculated, and then phellinus igniarius is acquired through cultivation, wherein the cultivation media are radix puerariae residues. The radix puerariae residues are used as the cultivation media, so that growth and reproduction of phellinus igniarius mycelia can be stimulated, the mycelia are stronger, germination and field planting of the phellinus igniarius mycelia are accelerated, and eventually the spawn running time can be shortened by about 15 d; in addition, invasion and infection of contaminating microorganisms can be reduced, and statistics show that the contamination rate of the material package can be reduced by 11.7% to 17.9% and the yield of phellinus igniarius can be increased by more than 15%.

The invention relates to a preparation method by using Chinese medicine residue as a cultivation material of edible mushroom pleurotus ostreatus, which uses the Chinese medicine residue, banana peels, bran, sawdust and lime as raw materials, and is prepared by crushing, insolating, proportioning the materials and sterilizing; specific steps are as follows: respectively crushing the banana peels, the bran and the sawdust into granules; placing the bran, the sawdust and the Chinese medicine residue outdoors to expose to the sun for sterilizing and dewatering; mixing the insolated bran, sawdust and Chinese medicine residue with the crushed banana peels, and then adding water and mixing with the lime sufficiently and uniformly, sterilizing at high temperature to obtain the cultivation material. The preparation method has advantages as follows: the waste Chinese medicine residue from medicine factories and the banana peels are used for culturing high-quality edible mushrooms, the waste is changed into things of value, the production cost is saved, the cultivation period is shortened, the yield is increased, the mushroom contamination rate is reduced with no poison or harm, the eating is safe, the waste treatment difficulty is resolved without causing pollution, both the environmental purpose and the economic purpose are achieved, the operation is simple, and the industrial production is easy to achieve.

The invention relates to a method for improving yield of straw mushroom, including: (1) raw materials are soaked; (2) the raw materials after being soaked are taken out and piled up, film is used for covering, heat preservation and moisturizing are carried out, and natural piling is carried out for fermentation; (3) the fermented culture material is moved onto a bedstead and covered by the film; (4) culture material is subject to secondary fermentation, and ignited honeycomb briquette is rapidly moved into indoor space, so as to carry out pasteurization; (5) microorganisms are uniformly scattered onto the upper surface of the culture material under open environment; (6) the temperature of material is controlled to be 35-38 DEG C, and air temperature is controlled to be 30-32 DEG C; (7) 'fruiting water' is sprayed five days after planting, and sodium acetate solution is sprayed while 'fruiting water' is sprayed; (8) harvesting is carried out. The invention has the advantages that method is simple, cost is low, formation of straw mushroom primordium can be promoted, the quantity of straw mushroom primodium is increased, yield of unit area is improved by about 17.9% at least, contamination rate is reduced, and biological efficiency is high, thus being beneficial to practical popularization and application.

A roller washing machine with a detachable inner barrel comprises a tank body; a tank body door is arranged on the tank body; an outer barrel door is arranged on an outer barrel; a magnetizer is fixed on a bottom flange of the inner barrel; a spline groove is formed in the magnetizer; the bottom of an outer barrel casing is provided with a carbon brush which supplies power for an electromagnet; a transmission mechanism which is arranged at the bottom of the outer barrel is mainly formed by a transmission shaft and the electromagnet; a sliding channel is arranged on the transmission shaft; the electromagnet is fixed at the front end of the transmission shaft; a spline is fixed on the electromagnet; the spline is connected with the spline groove. When the roller washing machine works, the electromagnet generates magnetism after being electrified, the inner barrel is adsorbed in the transmission mechanism, mutual mechanical transmission between the electromagnet and the magnetizer is correspondingly matched with axial positioning, or the transmission shaft and a groove of an inner barrel flange plate are connected in a matching mode to form into an axial positioning device, when the washing machine is required to be washed, power supply of the electromagnet is interrupted, the inner barrel is conveniently taken out to clean and sterilize the inner barrel and the outer barrel, the operation is convenient, the contamination rate is reduced, and cloth washing is clean and environment friendly.

The invention discloses a method for producing hypsizigus marmoreus in an industrializing way by applying liquid spawn, comprising used cultivating material formula, cultivating material water capacity and pH value, bottling weight and material level height, punching mode and bottle cap type, liquid spawn inoculation concentration, cultivating environment control parameters, cultivating period and post-ripening parameters, as well as mycelium stimulation and generation. The method is characterized in that the weight percent of the used cultivating material formula is as follows: 35-45 percent of corn cob, 20-35 percent of cotton seed hull, 5-15 percent of rice bran, 5-15 percent of wheat bran, 3-8 percent of corn flour, 3-8 percent of soy hulls, and 1-2 percent of calcium carbonate or lime or a mixture of the calcium carbonate and the lime; and the calcium carbonate and the lime are mixed by the weight ratio of 1:1 in the mixture of the calcium carbonate and the lime. The method shortens the production period of the hypsizigus marmoreus, controls contamination rate within 1 percent, improves quality and trait and saves cost by over 20 percent.

The invention provides a preparation method for an edible fungus culture medium. The preparation method comprises the following steps: 1) adding water mulberry branch powder to perform enzymolysis; 2) adding a compound enzyme preparation and uniformly mixing, fermenting and performing enzymolysis at the room temperature; and 3) adding 28-42 parts by weight of cotton seed hulls, 14-20 parts by weight of cassava vinasse, 12-18 parts by weight of bran, 2-5 parts by weight of lime, 1-5 parts by weight of gypsum and 1-5 parts by weight of calcium superphosphate into 42-78 parts by weight of mulberry branch powder obtained in the step 2) in sequence. An edible fungus cultural method is characterized by comprising the following steps: 1) adding a nutrient solution; 2) sterilizing; and 3) inoculating and culturing funguses. The edible fungus culture medium is low in contamination rate, high in fungus growing speed, good in mushroom-stick quality, thick in hypha growth, strong in growth power, good in receiving luminosity and good in disease resistance.

The invention relates to an industrialized production method of pleurotus eryngii. The industrialized production method of the pleurotus eryngii comprises the steps of strain cultivation, compost preparation and fungus bag manufacturing, inoculating, fungus germination management, bud trimming management, harvesting and packaging. The industrialized production method of the pleurotus eryngii has the advantages that bagasse is used as one of the main materials for the cultivation of the pleurotus eryngii, the range of cultivation materials of the pleurotus eryngii is widened, using capacity of agriculture waste is enlarged, environment pollution is reduced, extension of the industrial chain of sugarcane production is promoted, and due to the fact that the industrialized production method of the pleurotus eryngii can not be influenced by natural factors easily, the pleurotus eryngii can be produced all year round, efficiency is increased by more than 60% compared with the efficiency by adopting a traditional cultivation method, biological efficiency can reach to 60-80%, fungus bag inoculating efficiency is improved due to the fact that battens coated with parent fungi are adopted for the inoculating of the fungus bag, the success rate of the fungus bag inoculating reaches to more than 99.9%, contamination rate of the fungus bag inoculating is reduced, fungus germination is fast, time for the fungus germination of the fungus bag is shortened, and production efficiency is improved.

The invention provides a recombinant DNA sequence, hansenula polymorpha, a preparation method for hepatitis B surface antigen, and a hepatitis B vaccine. The recombinant DNA sequence is obtained by codon optimization of coding genes of the hepatitis B virus surface antigen according to codon usage frequency of the hansenula polymorpha. The invention also provides the hansenula polymorpha comprising the recombinant DNA sequence, a method for preparing adr sub-type hepatitis B surface antigen by using the recombinant DNA sequence, and the hepatitis B vaccine. The adr sub-type hepatitis B surface antigen has high expression level of the recombinant DNA sequence. The recombinant hansenula polymorpha is fast in growth speed, has high HBsAg yield, can be fermented in high cell density by using a cheap chemically synthetic medium, has low fermentation contamination rate and is beneficial to large-scale production; and HBsAg adr vaccine provided by the invention has high trend of Th1 and Th2 type cellullar immunologic response.

The invention belongs to the field of utilization of organic matters, and in particular relates to an edible straw rotting fungus culture medium and a preparation method thereof. The edible straw rotting fungus culture medium comprises the following components in percentage by weight: 40 to 50 percent of primary fungus chaff, 30 to 50 percent of xylitol residue, 5 to 20 percent of bran, 0.2 to 2 percent of calcium superphosphate, 0.2 to 1 percent of corn flour and 0.5 to 1 percent of gypsum. Lime milk and water are added into the medium, the pH is adjusted to be 8 to 10, and the water content is controlled to be 55 to 70 percent. The edible fungus culture medium is high in hypha running speed, hypha exuberantly grows, and the sundry fungus contamination rate can be greatly lowered; produced edible fungi have various nutritional characteristics of edible fungi produced by an ordinary culture material, and have a high edibleness and medicinal value.

The invention relates to pseudomonas aeruginosa and an application thereof. The pseudomonas aeruginosa is named as Pseudomonas aeruginosa YM4 according to bacterial strain classification, and has a preservation number of CCTCC NO:M 2017494. The bacterial strain can produce rhamnolipid in a high yield from hydrophilic carbon sources, hydrophilic carbon sources and composite carbon sources, and therhamnolipid has a substrate conversion rate reach 65-80%. In a rhamnolipid product of the YM4 bacterial strain, di-rhamnolipid and single rhamnolipid are main products, and the amount of di-rhamnolipid and single rhamnolipid takes 80-90% of the total amount of glycolipid. According to the carbon source metabolic characteristic of the bacterial strain, a foam-free seed medium formula suitable for industrial application is developed, and the contamination rate of a seed tank is greatly reduced. The bacterial strain is high in rhamnolipid yield, conversion rate and ratio of a diglycolipid component, and has high industrial application value.

The invention discloses a method for tissue culture and rapid propagation of Huzhou lily. The method comprises the following steps of: putting cleaned Huzhou lily scales into thiophanate methyl of which the concentration is 200-300mg / L, oscillating for 0.3 to 1 hour, soaking in 70-75% alcohol for 20 to 30 seconds, soaking in corrosive sublimate of which the mass volume concentration is 0.1-0.2% for 15 to 25 minutes, and rinsing out the scales; inoculating the rinsed-out scales to an induced medium to perform induced culture; after induced spouting, migrating sprouts into an enrichment medium to perform enrichment culture so as to obtain cluster buds; and inoculating the cluster buds to a strong-ball rooting medium to obtain aseptic seedlings. By adopting the method, the problem that explants of the Huzhou lily are easily subjected to fungal contamination and bacteria contamination during primary culture is solved, the contamination rate is controlled to be below 25%, and the death rate is low; and moreover, a wholes set of complete processes of primary culture, secondary culture, strong-ball culture and rooting culture is established and has high induction rate and multiplication multiple.

The invention discloses a rotary type nucleic acid extraction device and a control method thereof. The device comprises a multiflux extraction device, a plurality of extraction consumable components, a vertical device which is connected with the multiflux extraction device and a horizontal device which is connected with the extraction consumable components, wherein the multiflux extraction device adopts a rotary mode to mix samples and required extraction reagents uniformly, and the device can provide external force such as magnetic drawing, mixing and heating which is required by nucleic acid extraction and coordinate with a motion in a vertical and / or horizontal direction at the same time, so that the whole process of the nucleic acid extraction is achieved. According to the device, the blending efficiency of the samples and the required extraction reagents is improved, the cross contamination rate between extraction holes is lowered, and the length and the integrity degree of nucleic acid fragments in extraction product are guaranteed.

The invention provides a kiwi fruit tissue culture method and an application thereof. According to the method, axillary buds are germinated under an aseptic condition to form a leaf stalk serving as an explant, formation of callus tissues is induced, and then the callus tissues are cultured into kiwi fruit seedlings. According to the culture method, the contamination rate of a tissue culture process can be reduced, and the induction rate, the germination rate and the rooting rate of the callus tissues are increased. In addition, the kiwi fruit seedlings cultured by the method have the advantages of strong growth, developed root systems and high transplantation survival rate.

The invention provides a liquid fermentation medium for culturing edible mushroom liquid microbial strains. The liquid fermentation medium for culturing the edible mushroom liquid microbial strains comprises, by weight, 1.5% of glucose, 3% of corn flour, 0.2% of peptone, 2% of soybean meal, 0.1% of MgSO4 7H2O, 40.1% of KH2PO, 10.01% of VB, 0.03% of soybean oil and the balance water. The invention further provides an edible mushroom liquid microbial strain preparation method. The preparation method includes activation of inclining microbial strains, cultivation of first-level liquid microbial strains and liquid fermentation. According to the edible mushroom liquid microbial strain production method, through the schemes such as liquid medium formulation optimization and fermentation condition optimization, the yield of cultivation of the liquid microbial strains can be increased by 32.8% compared with that of bagging cultivation of solid microbial strains, the contamination rate is reduced by 4%, the seed production time is shortened, and the labor cost is reduced.

The invention relates to placenta preserving fluid with pH value of 7.33-7.83; the placenta preserving fluid per 1,000ml contains 10-15mmol of potassium dihydrogen phosphate, 30-40mmol of sodium chloride, 40-50mmol of potassium chloride, 8-10mmol of magnesium sulfate, 70-100mmol of histidine, 1.5-2.0mmol of allopurinol, 3mmol of reduced glutathione, 1-8ml of 2-mercaptoethanol, 8-10mmol of adenosine, 1,000,00-1,000,000U of penicillin, 60-100mg of streptomycin and 50-70mg of gentamicin. The placenta preserving fluid can store the delivered placenta for a long time, prevent from reduction of cell activity and control the bacteria contamination rate of the placenta to be in a low level.

The present invention relates to a strawberry detoxification tissue culture rapid propagation technology, and belongs to the technical field of agricultural bioengineering. The technology comprises: taking stolon tip of strawberry as explant, and carrying out inducing proliferation, secondary culture, rooting culture, transplanting acclimation and other steps so as to rapidly propagate detoxified tissue culture seedlings. According to the present invention, the formula of the culture medium is adjusted, and the stolon tip growth point is adopted to carry out inducing culture so as to achieve direct inducting budding without the callus growth stage, prevent and control varietal character variation and easily perform inoculation operation; characteristics of scientific sampling time, low inoculation contamination rate, high survival rate, simple, rapid and complete detoxification, rapidness, low cost, strong practicality and the like are provided, wherein the propagation coefficient achieves 5-8 times; and the technology is suitable for industrialized seedling breeding, and can be adopted for commercialized production.

The invention provides a method for improving the yield of saponin in panax notoginseng tissue culture seedlings by applying jasmonic acid methyl ester. The method comprises the following steps: establishing a fast breeding system of panax notoginseng tissue culture seedlings, applying jasmonic acid methyl ester to pseudo-ginseng tissue culture seedlings, cultivating pseudo-ginseng tissue culture seedlings and applying and spraying jasmonic acid methyl ester to the leaf surfaces of pseudo-ginseng tissue culture seedlings. The method is simple, convenient and easy to implement, can enable a great number of good pseudo-ginseng fast breeding seedlings to be formed in a short time, and has the advantages of high breeding speed and short period and the like; the externally applied jasmonic acid methyl ester can effectively improve the yield of total panax notoginseng saponins by 15 times within 5-10 days, and can improve the yield of main monomer ginsenoside by 2-5 times. According to the method for improving yield of saponin in panax notoginseng tissue culture seedlings, the yield of notoginsenoside can be effectively improved, the panax notoginseng tissue culture seedlings are bred by adopting a solid cultivation method, expensive fermentation equipment is not needed, the investment is reduced, the culture method is simple to operate, the breeding coefficient is high, the breeding speed is high, the contamination rate is low, seasonal limitation is avoided, the production cost of notoginsenoside is effectively reduced, and a practical guiding significance is provided for mass production of notoginsenoside.

The invention provides a selenium-rich culture medium for hericium erinaceus. The selenium-rich culture medium for hericium erinaceus is mainly prepared from wood flour, bran, selenium-rich fungus grass and gypsum. The invention further provides a method for producing hericium erinaceus with the selenium-rich culture medium for hericium erinaceus. A traditional production method of a hericium erinaceus strain is changed, a liquid strain technology is utilized in hericium erinaceus strain production, and a selenium-rich liquid strain is prepared; meanwhile, the solid selenium-rich culture medium and a secondary selenium enrichment method are adopted, and produced hericium erinaceus is high in selenium content and high in nutritive value. Production efficiency is improved, and production cost is greatly reduced; besides, the production method is simple in process, and the produced strain is high in utilization rate and low in contamination rate.

The invention discloses a juvenile form treating method of a mature citrus explant material. The juvenile form treating method comprises the following steps of: (1) repeatedly cross-grafting mature citrus scions on infantile stocks; (2) obtaining the explant material; and (3) regenerating adventitious buds. The juvenile form treating method through which the mature citrus scions are repeatedly cross-grafted on the infantile stocks is applicable to citrus varieties; the contamination rate of the treated mature citrus material is low, or even is 0; and the regeneration rate of the adventitious buds is high, and the induction rate of the adventitious buds is as high as 67.7%, so that the juvenile form treating method is particularly applicable to juvenile form of the mature citrus explant material of citrus varieties such as candy orange and sugar orange.

The invention discloses a morchella esculenta bacterial strain and a culture method thereof. The culture method of the morchella esculenta M-02# bacterial strain with a preservation number of CGMCC (China General Microbiological Culture Collection Center ) No.7058 is as follows: inoculating a mycelium of the morchella esculenta bacterial strain into a test tube culture medium inclined surface which contains an improved PDA (Potato Dextrose Agar) culture medium for culturing for 5 days-7 days under 18 DEG C-24 DEG C to obtain a test tube strain; inoculating the test tube strain into a triangular flask which contains a liquid culture medium for culturing on a shaking bed at 20 DEG C-26 DEG C, and culturing for 5 days-7days at rotation speed of 120 r / minute-160r / minute to obtain a primary liquid strain; and inoculating the primary liquid strain into a fermentation tank, ventilating for culturing for 72 hours-96 hours to obtain a liquid strain of the bacterial strain. The bacterial strain disclosed by the invention has mycelium biomass live-weight as high as 25g / kg, strong sclerotium generating capacity, pollution resistance, a low culturing contamination rate not greater than 2%, and short liquid fermentation time of 72 hours-96 hours, so that an excellent strain is provided for morchella esculenta wild resource protection and reproduction promotion.

The invention discloses a method for inversing railway base track bed contamination rate by using a ground penetrating radar. The method includes the following steps that: original data acquired by a vehicle-mounted ground penetrating radar are preprocessed; sectional imaging is performed on the pre-processed data according to track mileage intervals, so that two-dimensional imaging results of each section interval can be obtained; imaging results of a certain set depth interval at mileage points in each section interval are extracted; the energy values of the imaging results of the depth interval are calculated, and are adopted as railway base track bed contamination rates corresponding to the mileage points; and the railway base track bed contamination rates corresponding to the mileage points in each section interval are averaged, and an obtained result is adopted as the estimated value of the railway base track bed contamination rate of the corresponding section interval. The method of the invention has the advantages of high calculation speed and low relative error.

The invention relates to a method for genetically transforming citrus mature internode stems, which comprises following steps: firstly, disinfecting mature materials, selecting semi lignified new shoots in a greenhouse, using sodium hypochlorite solution 5wt% to wipe the shoots once every two days for a week before the shoots are sampled, wiping for 3-4 times together, then, cutting the shoots which are wiped with the sodium hypochlorite solution, cutting the shoots into stems of 4-5cm under the aseptic condition, using the sodium hypochlorite solution 10wt% which is added with tween 2-3 drops to dip for 20-35min, washing with sterilized water for 3-4 times, cutting the shoots into the internode stems of 1-1.5cm to preserve for use, secondly, transforming, thirdly, regenerating resistant buds, fifthly, grafting for the second time in the greenhouse, sixthly, identifying the resistant buds and resistant plants, and adopting a public-known PCR analysis method to identify. The citrus mature materials of the invention have good disinfection effect and low contamination rate, the adventitious bud induction frequency reaches 48%, the grafting survival rate of the resistant buds reaches more than 80%, and regenerated transformed plants do not have chimerism. The method is in particular suitable for genetically transforming mature internode stems of main cultivars of citrus such as crystal sugar oranges, navel orange, Ponkan and the like.

The invention discloses a method for preparing anti-tumor combined immune cells DC (dendritic cell)-CIKs (cytokine induced killers) and NKs (natural killers) simultaneously and the prepared combined immune cells. Ficoll density gradient centrifugation is used for efficient separation, a mononuclear cell is obtained, sufficient quantities of DCs, CIKs and NKs are obtained through cell culture bags and an immune cell induction culture system, and finally, the induced cells are cultured in a combined manner and applied to clinical treatment, so that a tumor killing effect is realized. The DCs, the CIKs and the NKs are subjected to induction culture respectively with adoption of TexMACS immune cell culture media produced by Miltenyi Biotec, autoserum, various cytokines and a combined culture technology, the cells are mixed for culture and application at certain point in time, application of fetal calf serum is avoided, the pollution rate of an exogenous pyrogen and an exogenous allergen is reduced, and the tumor killing activity of the finally mixed cells is enhanced simultaneously; with adoption of a cell culture bag technology, the cell contamination rate is reduced, and the method is suitable for clinical treatment and application.

The invention relates to a tissue culture propagating method of Tilia miqueliana Maxim, which pertains to the technical field of ligneous plant plantlet vegetative propagation. The invention aims at solving the problem that both seed seedling raising (low seed germination rate and long dormancy stage) and cottage propagation of the Tilia miqueliana Maxim are difficult. The method comprises the following steps: the stem sections with axillary buds at the different ages are taken as explants which are respectively inoculated on inducing culture medium, the best explant is selected based on the bud inducing result; and the best disinfection method is selected by comparing the contamination rates of different disinfection ways; an optimal bud inducing culture medium and an optimal successive propagation culture medium are respectively screened by adopting an L9(3<3>) orthogonal experimental design; based on the successive culture medium, 12 culture media are prepared by adjusting the hormone levels; the best rooting sound plantlet hardening culture medium is screened according to rooting situation. By adopting the experiment, the best tissue culture propagating method of the Tilia miqueliana Maxim is obtained by optimization.

The invention provides a sexual propagation method of bletilla striata. The sexual propagation method of bletilla striata particularly comprises the steps of processing of capsules, preparation of nutrients, inoculation and cultivation, and bottle out domestication, and is characterized in that in the step of processing of capsules, an alcohol lamp drying mode replaces a filter paper sucking dry mode in the prior art; in the step of preparation of nutrients, combination of banana slurry and humus is adopted; in the step of inoculation and cultivation, only inoculation, germination cultivation and strong seedling cultivation steps are included, the conventional enlargement cultivation process in an existing tissue culturing technology is omitted, and the process is simplified and the cost is lowered while the purposes are achieved; in the step of bottle out domestication, cultivation is performed through humus in a greenhouse, domestication is finished under the condition of controlling the relative humidity, temperature and shading rate, and qualified bletilla striata seedlings are obtained. Bletilla striata seeds are used for scale seedling culturing, the survival rate is high, manual scale propagation of bletilla striata is achieved, and the obtained bletilla striata seedlings are low in contamination rate, high in differentiation rate and stable in inheritable character.

The invention relates to a tissue culture method for cultivating amaryllis vittata by utilizing bulbs. The tissue culture method comprises the following steps: selecting and disinfecting explants; performing induction culture; performing subculture multiplication culture; rooting; transplanting. According to the invention, small bulbs of amaryllis vittata are taken as explants, so that the reduction of contamination rate is benefited and the induction rate is increased; cluster buds can be induced without callus stage; the cluster buds can be directly induced so as to acquire bulbs; the induction time is shortened; the buds can be added for multiplication culture; the multiplied test bulbs are rooted; one-step bulb forming of amaryllis vittata bulbs can be realized according to the invention; the time of tissue culture is saved; the operation steps are reduced; the excellent natures of original bulbs are kept; an effective method is supplied for industrial efficient cultivation of amaryllis vittata; a technical support is supplied for the industrial development of amaryllis vittata.

The invention provides a method for preparing decellularized cornea. Due to the selection of components and proportions, colloid osmotic pressure of a decellularized cornea preparation liquid can be maintained by using a prepared protection liquid, and compared with a conventional decellularized cornea preparation liquid, the decellularized cornea preparation liquid is capable of remarkably alleviating the situation that the cornea transparency is degraded as an extracellular matrix such as collagen is excessively swelled and lost in the later cell treatment process; in addition, due to the addition of antibiotics such as tobramycin, bacterium propagation of susceptible cornea can be inhibited, and the contamination rate in the treatment process can be reduced. By adopting the method provided by the invention, cells can be crushed, and the situation that the trenchancy is degraded as cornea matrix collagen is damaged by long-term high pressure can be avoided. Secondly, as a detergent and nuclease act up simultaneously, cell debris and nucleic acid components can be relatively rapidly and effectively removed, and the situation that the trenchancy of the cornea is degraded as protein components such as cornea collagen are damaged in long-term treatment of the detergent and other components can be avoided.

The invention relates to the technical field of industrialized fungus cultivation, in particular to a lentinula edodes strain suitable for industrialized cultivation and an industrialized cultivationmethod of the lentinula edodes strain. The lentinula edodes strain suitable for the industrialized cultivation is collected in CGMCC (China General Microbiological Culture Collection Center) with thecollection number of CGMCC 16919 on December 25th, 2018, and the name is Qihe 17. The industrialized cultivation method of the lentinula edodes strain suitable for industrialized cultivation comprisessteps as follows: preparation of strains, production of fungus sticks or tied fungus bags, inoculation, preculture, culture and fruiting management. The problems of high contamination rate, unstableyield and reduction of fruiting quality in traditional lentinula edodes stick production are solved, biotransformation rate of two crops of lentinula edodes is 70%-83%, and 98% or more of lentinula edodes sticks can realize normal budding and fruiting, fruiting is neat, the lentinula edodes is in middle-large size, and flower shape is round and normal.

The present invention discloses an anoectochilus roxburghii tissue culture seedling method, mainly comprises: A. culture medium preparing, wherein the step A comprises two steps of preparation and sterilization; B. inoculating, wherein a pre-treated explantation is defoliated under a sterile condition, a base stem segment with one knob is cut from each explantation and inoculated on the prepared culture medium; and C. culturing, comprising culturing in dark and culturing in light. According to the present invention, mashed sweet potato is coordinated with Hyponex 1, Hyponex 4 and Hyponex 5, the culture medium is prepared by a reasonable proportion to promote inducement and growth of anoectochilus roxburghii seedlings; the seedlings in a culture flask is free from transfer, thus operation steps are reduced, labour cost is lowered, a contamination rate is reduced, and a seedling rate is high, wherein a bud occurs within 15 days, rooting occurs after one month, and a common tissue culture seedling is produced after four months; the seedling rate is over 94%, and the survival rate of transplantation after two months is over 97%; and therefore a production period of the anoectochilus roxburghii seedling is shortened, and a rapid seedling culturing is prompted.

The invention discloses a method for producing stock seeds of enoki mushrooms. The method includes preparing culture media comprising 50-60% of sawdust, 34-45% of rice bran, 4-10% of cottonseed hulls and 0.5-2% of light calcium carbonate and enabling the moisture content of the culture media to be 62-64%; bottling, coring and covering the culture media in an environment with the room temperature lower than 30 DEG C, sterilizing the culture media at the temperature of 110+ / -2 DEG C under the pressure of 0.1-0.12Mpa for 60 minutes, keeping the culture media at the temperature of 121+ / -2 DEG C under the pressure of 0.12-0.13Mpa for 60-90 minutes, and feeding sterilized strain bottles into a cooling chamber to realize forced cooling for the strain bottles until the temperature of each strain bottle ranges from 16 DEG C to 20 DEG C; selecting second-level strains, inoculating the stock seeds of the second-level strains via a solid strain machine; culturing the stock seeds under conditions that the temperature ranges from 17 DEG C 20 DEG C, the humidity ranges from 65% to 70% and the concentration of carbon dioxide ranges from 1000ppm to 2000ppm after the stock seeds are inoculated; and selecting the stock seeds thrice in a culture process and culturing the stock seeds for 16-19 days before the stock seeds can be used. The inoculation amount of the stock seeds is proper so that a material surface can be covered. The method has the advantages that mycelia of the stock seeds are healthy, strong, white and vigorous, and are high in activity, and the strains are not easy to degenerate and are short in growth cycle; the enoki mushrooms can germinate quickly after inoculation and are low in contamination rate and high in yield; and the manufacturing cost is low, and technological conditions are easy to control.