16 results about "Contamination rate" patented technology

The invention relates to the technical field of seedling raising, and discloses an efficient tissue culture and rapid propagation seedling raising method for xanthoceras sorbifolia bunge, which comprises the following steps: selecting and pretreating explants, selecting current-year robust branches of the xanthoceras sorbifolia bunge, cutting stem segments with axillary buds as the explants, cleaning, trimming and disinfecting the explants, performing surface disinfection on the pretreated explants, and performing tissue culture and rapid propagation seedling raising on the pretreated explants to obtain the xanthoceras sorbifolia bunge. When the Xanthoceras sorbifolia Bunge is subjected to efficient tissue culture and rapid propagation seedling culture, an operation standard considering thorough sterilization and tissue activity protection is established by standardizing an explant selection standard and executing a step-by-step surface disinfection procedure, so that the microbial contamination rate caused by incomplete disinfection of the explant can be reduced, meanwhile, tissue activity damage caused by excessive disinfection is reduced, and the survival rate of the Xanthoceras sorbifolia Bunge is improved. Therefore, sufficient and healthy sterile initial materials are guaranteed to be obtained, the problems of high primary culture pollution rate and induction starting failure are solved, and a reliable foundation is laid for establishment of a whole tissue culture and rapid propagation system.

The present application provides a primer and probe combination for detecting rcAAV5 and use thereof. When detection is performed by the primer and probe combination of the present application, the contamination rate of rcAAV5 in rAAV5 can be detected quickly, sensitively, accurately and at low cost.

The application discloses a high-quality and high-yield cultivation method of wasp pupa cordyceps, and belongs to the field of artificial cultivation technology of rare medicinal and edible fungi. The method selects frozen wasps at each day age of mature larva stage to metamorphic stage as main raw materials of culture medium, and the culture medium is comprehensive in nutrition and high in quality. The cordyceps cultivated by the method contains high medicinal and nutritional components such as cordycepin, amino acid and cordyceps polysaccharide, and the content of cordycepin is particularly outstanding. The application has high safety and is not affected by environmental factors. The application adopts a pipette injection method, and the culture medium is prepared through normal pressure sterilization or high pressure sterilization. The number of wasp pupae inoculated per hour is 20 times that of the prior art, and the bacterial contamination rate can reach 100%. The application solves the problems of labor-intensive, time-consuming and low bacterial contamination rate of the current manual injection method, shortens the time for the insect body to be fully covered with bacteria by more than one week, and increases the yield by more than 3 times. The application is convenient for large-scale production, and has novelty, creativity and wide application value.

The present application belongs to the technical field of truffle planting, and particularly relates to a truffle planting formula and a planting method for shortening the initial yield period of truffles. In view of the problems of poor growth and development of mycorrhizal roots, poor mycorrhizal root vitality, small number of mycorrhizal roots, high contamination rate of ectomycorrhizal miscellaneous bacteria, long initial yield period of truffles, and low yield, the present application provides a truffle planting formula and a planting method. The formula can effectively promote the growth of truffle seedlings, the development of truffle mycorrhizal roots, reduce the contamination rate of ectomycorrhizal miscellaneous bacteria, accelerate the formation of fire rings, shorten the initial yield period of truffles by 2-5 years, and improve the yield of truffle planting.

This application provides an open tissue culture method for *Gnaphalium affine*, comprising the following steps: S1: sterilizing *Gnaphalium affine* explants to obtain pretreated explants; S2: inserting the pretreated explants into a first modified MS medium for a first-stage culture to obtain first-stage explants; S3: transferring the first-stage explants to a second modified MS medium for a second-stage culture to obtain second-stage explants; S4: transferring the second-stage explants to a third modified MS medium for a third-stage culture to obtain primary seedlings. By sequentially adding cell-free fermentation filtrate and PPM to the three-stage modified MS medium, efficient culture of *Gnaphalium affine* explants in an open environment is achieved, reducing contamination rate and browning degree, while promoting explant healing, adventitious bud differentiation, and root primordia formation, thus improving the seedling survival rate of open tissue culture of *Gnaphalium affine*.

A vehicle contains a sensor which in turn contains a receiver, the sensor being configured to generate an output. A controller is communicatively coupled to the sensor. The controller contains memory and a processor. The memory stores instructions for determining an object blocking rate of the output and for detecting a contamination rate of the receiver, at least partially based on the blocking rate of the output.

The invention discloses a method for detecting the Ag-110m contamination resistance of a coating. The method comprises the following steps: detecting radiation data when the surface of the coating of a base material is not contaminated; detecting radiation data when the surface of the coating of the base material is stained with Ag-110m; flushing the contamination on the surface of the coating of the substrate; detecting radiation data of the surface of the coating of the flushed and contaminated base material; and calculating the anti-contamination rate of the surface of the coating of the base material according to the radiation data when the surface of the coating is not contaminated, the radiation data when the surface of the coating is contaminated by Ag-110m and the radiation data after the surface of the coating is washed and contaminated. According to the detection method for the anti-Ag-110m contamination performance of the coating, the anti-Ag-110m contamination performance of the coating can be conveniently obtained.

The invention discloses a production process of an alcohol tricholoma matsutake fungus bag, which realizes standardized production of the fungus bag by optimizing the raw material ratio (70-80 parts of sawdust, 15-25 parts of wheat bran and the like), innovating a four-stage sterilization method (preheating at the temperature of 55 DEG C, sterilizing at the temperature of 125 DEG C and cooling in a gradient manner) and a closed liquid inoculation technology. According to the process, the biotransformation rate of the fungus bags is increased to 1: 1.8, the single-stick productivity is 0.8-1.2 kg, the pollution rate is reduced to 0.3%, the technical problems of insufficient raw material standardization, high sterilization energy consumption, high pollution rate and the like in the traditional process are solved, and reliable guarantee is provided for industrial cultivation of the mellow tricholoma matsutake.

The application discloses a kind of detection methods of carry pollution rate of cleaving instrument, it is related to cleaving instrument carrying pollution detection technical field.This method first takes 3 test tubes and is filled into marker solution, 3 test tubes are filled into equal amount of purified water;The test tube is sequentially placed on the sample holder of cleaving instrument, places 6 empty flow tubes on sampling tray, completes sampling;Liquid in 6 the flow tubes is directly (or after dilution) added to counting plate, and the counting under microscope is respectively i1, i2, i3;Take the liquid of proper amount in the flow tube filled with purified water and add to counting plate, and the counting under microscope is respectively j1, j2, j3;The above sampling and counting process is repeated several times;According to formula [(j3-j1) / (i3-j3)]×100%, the carry pollution rate of cleaving instrument is calculated in each repeated time, take maximum value, obtain the carry pollution rate of cleaving instrument.The test result of this method is little different from existing detection method, and the reliability is very high.

This invention relates to the field of boiler technology and discloses a method and system for controlling soot blowing on the heating surface of a thermal power plant boiler. The method includes: acquiring an infrared temperature image of the boiler heating surface and dividing the image into several temperature regions; acquiring current boiler operating parameters and determining the ideal temperature threshold for each temperature region based on these parameters; acquiring the actual temperature change trend of each temperature region and selecting regions to be soot blown based on the actual temperature change trend and the ideal temperature threshold; determining the contamination rate of each region based on its actual temperature change trend; and determining the soot blowing time based on the contamination rate of each region. This invention can accurately identify the heating surface regions requiring soot blowing, preventing both over-blowing and under-blowing, thus improving the soot blowing efficiency of the boiler heating surface.

The invention relates to a track bed smudginess rate detection method and device, equipment, a storage medium and a program product. The method relates to the technical field of rail transit, and comprises the following steps: based on point cloud data of a plurality of ballast beds, respectively generating three-dimensional point cloud models of the ballast beds; the point cloud data is acquired based on laser radar equipment in advance; on the basis of the three-dimensional point cloud models, feature parameter sets of the multiple ballast beds are obtained; the characteristic parameter set comprises the surface height distribution, the gravel gap density and the gravel particle size distribution condition of each ballast bed; training a preset machine learning model to obtain a smudginess rate detection model based on the feature parameter set and pre-acquired smudginess rate label data of a plurality of ballast beds; and inputting the characteristic parameters of the to-be-detected ballast bed into the smudginess rate detection model to obtain the smudginess rate of the to-be-detected ballast bed. By adopting the method, the accuracy of a track bed smudginess rate detection result can be improved.

The invention discloses an old wine pretreatment device for online quality inspection, and belongs to the technical field of wine production. The device comprises a barrel body, a fermented grain beating module, a material conveying device, a wine aging tank and a quality inspection system. The fermented grain beating module is arranged in the barrel body and comprises a transmission device and an auxiliary cleaning device, stirring paddles which are alternately mounted in a forward and inverted manner are arranged on the transmission device, and the rotating directions of the adjacent stirring paddles are opposite to form strong shearing and rubbing effects; a scraper of the auxiliary cleaning device is tightly attached to the barrel wall and rotates along with the power shaft to scrape attachments on the barrel wall, and the problems that fermented grains are not fully crushed and the machine wall is attached are solved. The material conveying device is provided with a cooling pipe and a flow guiding device, and steam cooling and liquid material conveying are achieved. The quality inspection system comprises a monitoring module and a microbiological detection module, parameters such as temperature, humidity, pH value, flora proportion and the like are monitored in real time in the fermented grain beating process, comparison and judgment are performed through a data processing and analysis module, a control and feedback module generates a control instruction for real-time regulation and control, closed-loop control is formed, and the quality inspection efficiency is improved. Compared with a traditional mode, the crushing uniformity is improved by 17%, the infectious microbe contamination rate is reduced by 80% or above, and the stability and the production efficiency of fermented grain products are remarkably improved.

A vehicle includes a sensor including a receiver, the sensor configured to generate an output. The controller is communicatively coupled with the sensor. The controller includes a memory and a processor. The memory stores instructions for determining an output object occlusion rate and detecting a contamination rate of the receiver based at least in part on the output occlusion rate.

A vehicle includes a sensor including a receiver, the sensor being configured to generate an output. A controller is communicatively coupled with the sensor. The controller includes a memory and a processor. The memory stores instructions for determining an object obstruction rate of the output and detecting a contamination rate of the receiver based at least in part on obstruction rate of the output.

The invention provides an induction method and application of tea tree leaf calluses. A method for inducing calluses of tea tree leaves comprises the following steps: selecting mature leaves of a tea tree, reserving petiole parts, cleaning for later use without disinfection, inoculating an obtained explant to an induction culture medium for induction culture, the induction culture medium being selected from any one of an MS culture medium, 2.5 mg / L of 6-BA and 0.1 mg / L of NAA; the MS culture medium is prepared from 1.5 mg / L of 6-BA, 0.5 mg / L of zeatin and 0.1 mg / L of IBA; the MS culture medium is prepared from 2.0 mg / L of zeatin and 0.1 mg / L of IBA. According to the method, it is found for the first time that cane sugar is a key cause of infectious microbe breeding in tea tree tissue culture, by removing cane sugar in the culture medium, the culture medium can be kept sterile for a long time even in an open environment (no bacteria grow for five months), the industrial problem of tea tree endophyte and infectious microbe pollution is successfully solved, and the pollution rate is almost zero.

This invention relates to the field of silkworm production technology, specifically to a strain of *Beauveria bassiana* for producing silkworms, its preparation method, and its application. The strain is named *Beauveria bassiana* strain SJ922, with accession number CCTCC NO: M 20252207, and its ITS sequence is shown in SEQ ID NO: 1. This strain exhibits specificity and high-efficiency infection ability against silkworms, significantly shortening the molting cycle and increasing the cumulative mortality rate. The wet and dry weights of silkworms prepared using this strain are significantly better than those of the control strain, and the product has high purity and low contamination rate, effectively ensuring the uniformity and safety of the silkworms. This invention also provides a method for preparing this strain and its application in silkworm production, providing an excellent strain resource for the standardized and large-scale production of silkworms.