Tissue culture seedling method for top shoots of passiflora caerulea

Abstract

The invention provides a tissue culture seedling method for top shoots of passiflora caerulea. The method comprises technical links as follows: anti-browning and disinfection treatment of explants, initial induced culture of axillary buds, induced culture of callus, multiplication of subculture, induced culture of rooting and bottle seedling transplanting; tender top shoots are taken as the explants, soaked with an antioxidant Vc and citric acid mixed solution and subjected to soaking sterilization with a potassium permanganate solution, and anti-oxidative and anti-browning capacity and activity of the tender explants are improved; activated carbon and polyvinylpyrrolidone are added to culture media of the stages of initial induction of the axillary buds, induction of the callus and multiplication of subculture and used for adsorbing secreted harmful substances and inhibiting production of browning substances. With adoption of the method, contamination rate and browning rate of initialinduction of the top shoots of passiflora caerulea can be controlled to be 10% or lower; initial induction survival rate of the top shoots reaches 90% or higher, subculture multiplication coefficientof cluster buds is 5.0 or larger, and rooting rate is 90% or higher; survival rate of bottle seedling transplanting reaches 95.0% or higher. The method can meet the demand of industrialized seedlingproduction.

Description

technical field [0001] The invention relates to crop cultivation techniques, in particular to a method for group seedling cultivation of purple passion fruit top shoots. Background technique [0002] Passiflora (scientific name: Passiflora caerulea L.), a perennial evergreen climbing woody vine, is a fragrant and delicious fruit and has the reputation of "king of juice". Its fruit is rich in calcium, iron, phosphorus and other trace elements and 17 kinds of amino acids and other substances. In addition to eating, it also has good health care and medicinal effects. According to the determination, passion fruit fruit contains more than 135 kinds of aromatic substances, which are most suitable for processing into fruit juice. But the planting passion fruit disease is more serious, mainly contains root rot, phytophthora, anthracnose and mosaic disease etc., especially in the perennial orchard, the disease is more serious, causes dropped fruit and defective fruit to increase, an...

AI Technical Summary

Problems solved by technology

Existing detoxification methods for passionflower seedlings all have complex operating procedures, explants are all derived from mature organs or tissues that may carry viruses and bacteria, and the detoxification effect of single shoot tip heat treatment is unstable, etc.

The newly germinated top shoots of passionflower belong to young tissues with strong differentiation ability, almost no viruses and germs, and are easy to brown after disinfection treatment. At present, the young top shoots of passionflower are used as explants, and cell division is adopted. The combination of TDZ (thiadizuron) and auxin NAA to induce axillary bud differentiation, callus differentiation and clustered bud differentiation has not been reported.