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Tissue-culture seedling raising method for kadsura coccinea tender stems with buds

A technology of cold rice balls with buds, applied in gardening methods, botanical equipment and methods, gardening, etc., can solve the problems of no tissue culture, etc., and achieve the effect of improving vitality and anti-oxidation

Inactive Publication Date: 2018-06-22
贵州省山地资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the research on tissue culture technology, and there is no application for tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Trial in April 2016

[0018] (1) Disinfection treatment of explants: collect 50 spring shoots of cold onigiri that have just germinated for 5 days, rinse them with tap water and distilled water 3 times, remove the leaves, and keep the tender stems with buds as explants. Use 3500mg.L -1 Soak in vitamin C distilled water for 50 minutes, rinse with distilled water 3 times. On the ultra-clean bench, use 3000mg.L -1 Soak in potassium permanganate distilled water for 30 minutes, rinse with distilled water 3 times. Cut the explants into 1.5cm long bud stem segments, and then use 1000mg.L -1 Mercury chloride aqueous solution disinfection for 10min, distilled water rinse 5 times.

[0019] (2) Primary induction culture of axillary buds: inoculate the sterilized explants upright according to the growth polarity, medium formula: basic medium + 3.0g.L -1 Perlite+2.0g.L -1 AC+1.5g.L -1 PVP+1.0mg.L -1 TDZ+0.01mg.L -1 NAA. A total of 50 buds were inoculated, and af...

Embodiment 2

[0024] Example 2 Test in September, 2016

[0025] (1) Disinfection treatment of explants: Collect 50 autumn shoots of cold rice balls that have just germinated for 10 days, rinse them with tap water and distilled water 3 times, remove the leaves, and leave the tender stems with buds as explants. Use 3500mg.L -1 Soak in vitamin C distilled water for 50 minutes, rinse with distilled water 3 times. On the ultra-clean bench, use 3000mg.L -1 Soak in potassium permanganate distilled water for 30 minutes, rinse with distilled water 3 times. Cut the explants into 1.5cm long bud stem segments, and then use 1000mg.L -1 Mercury chloride aqueous solution disinfection for 10min, distilled water rinse 5 times.

[0026] (2) Primary induction culture of axillary buds: inoculate the sterilized explants upright according to the growth polarity, medium formula: basic medium + 3.0g.L -1 Perlite+2.0g.L -1 AC+1.5g.L -1 PVP+1.0mg.L -1 TDZ+0.01mg.L -1 NAA. A total of 50 buds were inoculate...

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PUM

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Abstract

The invention relates to a tissue-culture seedling raising method for kadsura coccinea tender stems with buds. The method comprises the technical procedures of explant anti-browning treatment, disinfection treatment, axillary bud induction culture, callus induction culture, cluster bud induction differentiation and rooting culture. The method adopts the freshly germinated tender stems with buds asexplants, and the explants are soaked in antioxidant Vc distilled water solution and then soaked in potassium permanganate distilled water solution; finally perlite and activated carbon with large porosity are added in a three-stage culture medium of initial induction, callus induction and subculture proliferation culture to absorb the white mucus and harmful substances produced after splitting of the kadsura coccinea tender stems, and PVP is added to inhibit the production of browning substances such as phenols and the like. By adopting the method, the initial induction contamination rate and the browning rate can be controlled less than 15%, the value-added coefficient is 4.0 or above, and the induced survival rate and the rooting rate are both 85% or above. The tissue-culture seedlingraising method is systematically reported for the first time, and a reference is provided for system optimization and integration of a kadsura coccinea improved variety factory-like seedling raising technique.

Description

technical field [0001] The invention relates to crop cultivation techniques, in particular to a method for group seedling cultivation of cold rice balls with buds and tender stems. Background technique [0002] Schisandra plants have high medicinal value. The lagging seedling breeding technology is a technical bottleneck that urgently needs to be solved in the industrial development and utilization of plants of this family. At present, only a few species have carried out research on group breeding technology. Wu Lingna et al. (2007) conducted a study on the influencing factors of the callus induction of the young leaves of Schisandra chinensis, and the results showed that the combination of soaking the young leaves in 75% alcohol for 10 seconds and sterilizing with 0.1% mercury liter for 4 minutes had a better sterilization effect; The base is: MS+2,4-D0.5mg.L -1 +NAA0.1mg.L -1 +6-BA1.0mg.L -1 , The callus induction rate is as high as 96.7%. Hua Zhirui (2015) carried o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丽敏王济红席培宇蔡国俊彭熙李安定张建利龙秀琴
Owner 贵州省山地资源研究所
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