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Polyploid induction method for Chinese gooseberry Hort 16A offspring fine strain tissue culture seedling

A Chinese kiwifruit and polyploid technology, applied in the field of plant genetics and breeding, can solve problems such as long time, achieve the effects of low cost, meet consumer demand, and shorten the cultivation period

Inactive Publication Date: 2015-05-13
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the explants are leaf tissue cultures to produce callused petioles, and the culture medium needs to use expensive zeatin as a hormone, and the culture period of each step is 6 weeks, which takes a long time.

Method used

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  • Polyploid induction method for Chinese gooseberry Hort 16A offspring fine strain tissue culture seedling
  • Polyploid induction method for Chinese gooseberry Hort 16A offspring fine strain tissue culture seedling
  • Polyploid induction method for Chinese gooseberry Hort 16A offspring fine strain tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0037](1) Take the tissue-cultured seedlings of the Chinese kiwifruit variety ‘Hort 16A’ offspring, remove the leaves from the well-grown aseptic seedlings on an ultra-clean workbench, and cut them into 1cm 2 small pieces, soaked in the autoclaved 60mg / L colchicine aqueous solution (such as figure 1 ), treated for 30 hours, and washed once with sterile water after the treatment.

[0038] The tissue-cultured seedlings of the superior line of the Chinese kiwifruit variety 'Hort 16A' were cultivated by the Ministry of Education Key Laboratory of Southwest Mountain Forest Resources Conservation and Utilization of Southwest Forestry University.

[0039] (2) Transfer the soaked leaves to the proliferation medium without colchicine. The leaves should be flattened so that they are fully in contact with the medium, and the leaves should be exposed to the medium, and cultured in the light for 20 days After the callus grows, transfer the bottle to a new proliferation medium with the sa...

example 2

[0044] The strong polyploid seedlings after the subculture in step (3) were rooted and cultured, and the other steps were the same as in Example 1.

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Abstract

The invention relates to a polyploid induction method for Chinese gooseberry Hort 16A offspring fine strain tissue culture seedlings. The polyploid induction method comprises the following steps: soaking leaves of the Hort 16A offspring strain tissue culture seedlings in a colchicine water solution to induce polyploidy, washing the leaves, and inoculating in callus differentiation enrichment culture medium and subculture culture medium for light culture according to the steps. The polyploid induction method is simple in operation, good in induction directionality, high in induction success rate, low in cost, shorter in culture period and higher in induction rate, an efficient and feasible new method is provided for polyploidy breeding of Chinese gooseberry, the polyploid induction method has important application values, through the popularization of the polyploid breeding method, economic benefits can be brought for fruit farmers, and demands of more consumers are met.

Description

technical field [0001] The invention belongs to plant genetics and breeding, and relates to a method for inducing polyploidy of excellent strains of plants. Background technique [0002] Kiwifruit (kiwifruit) is a deciduous vine of Actinidiaceae (Actinidiaceae) and is an important economic forest and fruit tree. Kiwifruit is rich in Vc, minerals, anthocyanins and other nutritional and medicinal ingredients. These ingredients are at the forefront of other fruits, and their comprehensive economic value is usually 3 to 5 times higher than that of ordinary fruits. In recent years, as a new economic type of fruit, it is widely used in edible forms such as fresh fruit, beverages, and health care products, and its demand has increased. Accompanied by this development, the demand for seedlings of fine varieties of kiwifruit has increased sharply, and at the same time, the requirements have become higher and higher, including fruit flavor, novelty, size, flesh color, storage time, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张汉尧李升星张太奎刘小珍贺笑刘惠民陈湛陈家林段云霞
Owner SOUTHWEST FORESTRY UNIVERSITY
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