The invention relates to a method for inducing a chill-proof Aglaonema spp polyploidy, relating to an Aglaonema spp breeding technology, which comprises the following steps of: inducing cluster buds, colchicine treating, carrying out low-temperature treatment, propagating variation plants, identifying the polyploidy, rooting and transplanting, wherein after being subjected to five generations of propagation, the variation plants are subjected to polyploidy detection, thus, the proportion of a chimera can be effectively reduced; the stems of the polyploidy plants are thickened and stunted in comparison with common stems, thus, the shapes are more attractive; the polyploidy plants have the chill-proof property and can resist 5 DEG C without cold injury; the temperature increasing cost of a greenhouse in winter is reduced; and the economical benefit is greatly increased.