Method for cultivating aneuploid perennial forage grass variety by using corn heterologous polyploidy

A technology of allopolyploidy and aneuploidy, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problem of low seed setting rate of finger-shaped friction grass, poor palatability of grass quality, and low germination rate of seeds and other problems, to achieve the effects of strong winter resistance, good stress resistance, and expanded application range

Active Publication Date: 2014-02-05
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] It can be seen from the above that whether it is corn, tetraploid perennial teosinte or finger-shaped friction grass, they all have their own shortcomings when used as high-quality forage grass. For example, corn is an annual; The stems are aggressive, and the seeds are easy to fall off; the finger-shaped friction grass has low seed setting rate, dormancy, low seed germination rate, and poor palatability of grass quality; it seriously restricts the promotion and use of corn, teosinte and friction grass directly as forage grass

Method used

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  • Method for cultivating aneuploid perennial forage grass variety by using corn heterologous polyploidy
  • Method for cultivating aneuploid perennial forage grass variety by using corn heterologous polyploidy
  • Method for cultivating aneuploid perennial forage grass variety by using corn heterologous polyploidy

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Embodiment 1

[0039] Example 1: Using corn allopolyploid to cultivate aneuploid perennial forage varieties

[0040] Follow the steps below:

[0041] (1) In the spring of 2007, MTF-1 (TripsazeacreammaizeT.2n=76; Su Yuegui, Sichuan Agricultural University master's thesis, 2009 when the ground temperature rises above 10℃ in Duoying Farm on Ya'an Campus of Sichuan Agricultural University. The public can obtain it from Sichuan Agricultural University) (see figure 1 ) Propagating separately, and planting the obtained seedlings to the field. At the same time, plant tetraploid perennis 9475 (Zea perennis 9475, 2n=40, referred to as 9475 for short) within 2-4 meters of MTF-1. Available for free from the Mexican Wheat and Corn Improvement Center),

[0042] (2) During the spinning and pollination period, any tetraploid perennial corn pollen will openly pollinate MTF-1 female ears (without bagging), harvest the hybrid ears after maturity, and store them in a refrigerator at -20℃.

[0043] (3) In the spring of...

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Abstract

The invention discloses a method for cultivating an aneuploid perennial forage grass variety by using a corn heterologous polyploidy and belongs to the field of corn distant hybridization. According to the method, MTF-1 (Tripsazea creammaize T.2n=76) cultivated by Sichuan Agricultural University is used as a female parent and teosinte is used as a male parent to be hybridized; a filial generation is selected from perennials, a wintering habit, a tiller number, a chromosome number and chromosome constitution to select the perennial heterologous aneuploid forage grass variety. According to the method, firstly, a heterologous aneuploid is used as the forage grass variety to be applied so as to creatively expand an application range of corn affinis species and explore a novel way for utilizing corn affinis specie materials; secondly, a material cultivated by the method is used as a bridge material and has a very great application value; furthermore, a vegetative propagation manner can be used for fixing a hybrid vigor and the variety is perennial, so that the production cost is very low, the yield and the nutritive value are high, and the economic benefit is great.

Description

Technical field [0001] The invention belongs to the field of maize distant hybrid breeding, and specifically relates to a method for cultivating aneuploid perennial forage grass varieties by using maize allopolyploidy. Background technique [0002] Dawei grass and cultivated corn are different subgenus in the same genus or different subspecies in the same species. In the genus Gemus (Gemus Zea), in addition to the cultivated subspecies of corn (ssp.mays), the remaining seven species or subspecies are collectively called "Teosintes" (Teosintes). Among the maize relatives, the relationship between Daminaria and maize is the closest, and crosses with maize can obtain fertile offspring. Different types of wild grasses live in different ecological environments and have unique characteristics. They are the most direct outer gene pool for broadening maize germplasm resources. Among the big stalks, tetraploid perennial stalks (also known as tetraploid perennial corn) (Zea Perennis, 2n=...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01G1/00
Inventor 唐祈林荣廷昭李华雄郑名敏程明军苏月贵周树峰吴元奇曹墨菊蒋伟
Owner SICHUAN AGRI UNIV
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