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A rice blast resistance gene rmg38 and its application

A rice blast resistance gene and rice technology, applied in the field of genetic engineering, can solve the problems of tedious and time-consuming breeding process, and achieve the effects of broadening the resistance spectrum, enhancing resistance and shortening the breeding cycle

Active Publication Date: 2017-05-17
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, only 23 blast resistance genes have been cloned so far
Although the genes that have been located can be utilized by molecular marker-assisted selection, the breeding process is cumbersome and time-consuming. After the new variety is bred, it may be susceptible to the newly generated mutant strain, cloned and directly transformed into a resistant strain. Disease gene may be the most direct and effective way to breed disease-resistant varieties

Method used

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  • A rice blast resistance gene rmg38 and its application
  • A rice blast resistance gene rmg38 and its application
  • A rice blast resistance gene rmg38 and its application

Examples

Experimental program
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Embodiment 1

[0022] Example 1 Cloning, vector construction and identification of resistant plants of rice blast resistance gene RMg38

[0023] The test procedure of this embodiment is as figure 1 Shown.

[0024] 1. Determination of the candidate site RMg38 for resistance to rice blast: (1) Most of them exist in the form of gene families and gene clusters. There are 4 and 2 similar copies in each of Nipponbare and 93-11; (2) in their LRR Areas, especially LxxLxLxx areas, have a higher Ka / Ks value;

[0025] 2. Isolation and cloning of rice blast resistance gene RMg38 (12g36730-Q2436): Use public databases to sequence the varieties Nipponbare and 93-11 as reference sequences, and design primers (both ends of the primers have restriction sites AscI) . The primer sequence is shown in the sequence table, the forward primer sequence is shown in SEQ ID NO: 4; the reverse primer sequence is shown in SEQ ID NO: 5.

[0026] Using disease-resistant rice varieties Tetep, Gumei No. 2 and Q2436 as templates, ...

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Abstract

The invention belongs to the field of a gene engineering technology and specifically relates to a rice blast resistance gene RMg38 and its application. The invention discloses a nucleotide sequence and its coding amino polypeptide sequence of the rice blast new resistance gene RMg38. The gene belongs to a member of NBS-LRR type resistance gene family. The rice blast resistance gene provided by the invention is cloned from the rice line which shows highly resistance to rice blast, and the rice blast resistance gene is transformed into rice varieties which are sensitive to rice blast pathogen. The disease resistance of the transformed rice is evaluated by infection of rice blast pathogen so as to finally determine that the gene has resistance to rice blast.

Description

Technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a rice blast resistance gene RMg38 and its application. Background technique [0002] Plant disease resistance genes are the result of long-term interaction between plants and pathogens, and they play a very important role in the evolution of plants. Therefore, the analysis and research of plant disease resistance gene structure is the basis for understanding the mechanism of plant disease resistance and has important guiding significance for the prevention and control of plant diseases. Since the first plant disease resistance gene Hm1 was isolated from maize by Johal and Briggs in 1992 (Tanksley et al. 2007; Dong Yuchen, 2001), there have been more than 80 plant disease resistance genes from different plants. It was cloned and isolated in the slab, and the main type of disease resistance gene is the NBS-LRR structure type (Nucleotide-binding site and leucine-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/113C12N15/82C12N1/21C07K14/415A01H5/00A01N47/44A01P3/00
Inventor 张小辉谢正清田大成杨四海
Owner NANJING UNIV
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