A rice blast resistance gene rmg42 and its application
A rice blast resistance gene and rice blast resistance technology, applied in the field of genetic engineering, can solve the problems of time-consuming and cumbersome breeding process, and achieve the effect of overcoming the difficulty of distant hybridization and shortening the breeding cycle.
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[0028] Cloning, vector construction and identification of resistant plants of rice blast resistance gene RMg42
[0029] The test procedure of this embodiment is as follows figure 1 as shown in figure 1 A-1H is shown.
[0030] 1. Determination of the blast resistance candidate site RMg42: (1) There is one similar copy in each of Nipponbare and 93-11; (2) It has a higher Ka / Ks value in its LRR region, especially in the xxLxLxx region ;
[0031] 2. Isolation and cloning of the rice blast resistance gene RMg42: using public database sequencing varieties Nipponbare (Nipponbare) and 93-11 as reference sequences, design primers (both ends of the primers have restriction sites AscI). The primer sequence is listed in the sequence table, the forward primer sequence is shown in SEQ ID NO:4; the reverse primer sequence is shown in SEQ ID NO:5.
[0032] Using disease-resistant rice varieties Tetep, Gumei 2 and Q2436 as templates, long-segment PCR (Long-PCR) was used to amplify candidate ...
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