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A rice blast resistance gene rmg42 and its application

A rice blast resistance gene and rice blast resistance technology, applied in the field of genetic engineering, can solve the problems of time-consuming and cumbersome breeding process, and achieve the effect of overcoming the difficulty of distant hybridization and shortening the breeding cycle.

Active Publication Date: 2021-06-22
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only more than 20 blast resistance genes have been cloned so far
Although the genes that have been located can be utilized by molecular marker-assisted selection, the breeding process is cumbersome and time-consuming. After the new variety is bred, it may be susceptible to the newly generated mutant strain, cloned and directly transformed into a resistant strain. Disease gene may be the most direct and effective way to breed disease-resistant varieties

Method used

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  • A rice blast resistance gene rmg42 and its application
  • A rice blast resistance gene rmg42 and its application
  • A rice blast resistance gene rmg42 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Cloning, vector construction and identification of resistant plants of rice blast resistance gene RMg42

[0029] The test procedure of this embodiment is as follows figure 1 as shown in figure 1 A-1H is shown.

[0030] 1. Determination of the blast resistance candidate site RMg42: (1) There is one similar copy in each of Nipponbare and 93-11; (2) It has a higher Ka / Ks value in its LRR region, especially in the xxLxLxx region ;

[0031] 2. Isolation and cloning of the rice blast resistance gene RMg42: using public database sequencing varieties Nipponbare (Nipponbare) and 93-11 as reference sequences, design primers (both ends of the primers have restriction sites AscI). The primer sequence is listed in the sequence table, the forward primer sequence is shown in SEQ ID NO:4; the reverse primer sequence is shown in SEQ ID NO:5.

[0032] Using disease-resistant rice varieties Tetep, Gumei 2 and Q2436 as templates, long-segment PCR (Long-PCR) was used to amplify candidate ...

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Abstract

The invention belongs to the technical field of genetic engineering, and in particular relates to a rice blast resistance gene RMg42 and its application. The invention discloses the nucleotide sequence of the rice blast new resistance gene RMg42 and the encoded amino acid polypeptide sequence. This gene is a member of the NBS‑LRR type disease resistance gene family. The rice blast resistance gene in the present invention is cloned from a rice line showing high resistance to blast fungus, and transformed into a variety susceptible to blast fungus, and its disease resistance ability is utilized by the method of infection by blast fungus Evaluation was carried out to conclusively confirm that the gene confers resistance to rice blast.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a rice blast resistance gene RMg42 and its application. Background technique [0002] Plants in nature are attacked by a wide variety of pathogenic bacteria during their growth and development. Plant diseases bring huge losses to agriculture and forestry production every year, so reasonable and effective prevention and control of plant diseases is one of the key issues that must be solved to ensure the sustainable development of agriculture and forestry. A large number of practices have proved that the development and utilization of existing plant disease-resistant germplasm resources is one of the most effective methods to prevent and control plant diseases (Tanksley et al. 2007; Dong Yuchen, 2001). [0003] During the long-term interaction between plants and pathogenic bacteria, plant resistance genes (Plant Resistance Gene, R gene) play an extremely im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/11C12N15/82A01H5/00A01H5/10A01N47/44A01P3/00A01H6/46
CPCA01N47/44C07K14/415C12N15/8282
Inventor 田大成张玉涵杨四海张小辉
Owner NANJING UNIV
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