21 results about "Corn pollen" patented technology

The application discloses application of ZmGOLS2 genes and ZmRAFS genes in improving pollen vitality of corn under heat shock and drought conditions, and belongs to the technical field of genetic engineering. The application improves the pollen vitality of corn, promotes the germination of pollen under heat shock and drought stress conditions, and reduces the empty stalk rate of corn under drought stress conditions by simultaneously improving the expression levels of a corn myo-inositol galactoside synthetase coding gene and a corn raffinose synthetase coding gene.

The invention discloses a corn kernel linoleic acid content negative regulation gene ZmRPL31 as well as a mutant and application thereof, and a candidate gene ZmRPL31 closely related to linoleic acid anabolism in a high-linoleic acid corn selfing line YM-2 is successfully screened based on BSA (Bovine Serum Albumin) population construction and RNA-seq transcriptome analysis. Corn B73 pollen is treated through an ethyl methanesulfonate mutagenesis technology, and a rpl31 homozygous mutant strain is successfully constructed. GC-MS detection is carried out on the linoleic acid content of a homozygous mutant strain, and it is confirmed for the first time that the ribosomal protein gene ZmRPL31 has a negative regulation effect on synthesis of corn linoleic acid. Wherein the linoleic acid content of the rpl31-22 homozygous mutant strain in grain tissues is remarkably increased by 43.7% compared with that of wild type corn B73, and meanwhile, the linoleic acid content of the rpl31-22 homozygous mutant strain in leaves in the seedling stage is remarkably increased by 23.5% compared with that of the wild type corn B73.

The application provides a corn breeding powder taking device, which comprises cover body one and cover body two, the top of the cover body one and the cover body two is respectively provided with top plate one and top plate two, the top plate one is provided with a semicircular notch, the top plate two comprises a semicircular plate corresponding to the semicircular notch, and the bottom of the cover body one and the cover body two is a semicircular opening; the top of the top plate one and the top plate two is provided with an elastic telescopic rod, the two ends of the elastic telescopic rod are respectively fixedly connected with the top plate one and the top plate two; the top plate two is provided with a pneumatic assembly, the pneumatic assembly comprises a cylinder, the side of the cylinder is provided with an air inlet and an air outlet, the cylinder is slidably provided with a piston and an elastic rod corresponding to the piston, the piston is provided with a guide rod, and the guide rod is provided with a support rod; the top plate one and the support rod are provided with a pull rope; the top plate two is provided with a powder beating assembly; the bottom of the cover body two is provided with a discharge port, the discharge port is connected with the inlet of an air blower, and the outlet of the air blower is connected with a collecting device through an air exhaust pipe. The application can efficiently collect corn pollen, and the device is ingenious in structure and easy to operate.

The application belongs to the field of genetic engineering, and discloses a maize pollen fertility regulation gene ZmPUB33 and an encoded protein. The gene regulates maize pollen aperture development and further mediates pollen fertility ability. After mutation, the gene cannot normally blossom, anther husks cannot basically crack, anthers are smaller than wild types, and almost not exposed, pollen grains are not active, and can cause complete male sterility of plants. The sterile line can be used for sterile seed production and production of hybrid crops, and has great application and economic value.

The invention discloses a method for improving the transformation efficiency of nano magnetic bead mediated maize pollen, which comprises the following steps: constructing Ac / Ds transposon elements of maize into plasmids, constructing complete expression cassettes of a herbicide selection marker gene and a target gene between 3 'Ds and 5' Ds of maize transposon, then cutting down fragments from 3 'Ds to 5' Ds by utilizing transposase AcTpase, and screening the fragments from 3 'Ds to 5' Ds to obtain the nano magnetic bead mediated maize pollen. The exogenous gene is integrated into the corn genome by using the transposon characteristics. According to the method, the average stable genetic efficiency reaches 9.6% through herbicide screening and identification, and compared with the original 1.41%, the transformation efficiency of the method is greatly improved.

The application provides a mobile corn pollen collecting device and relates to the technical field of corn pollen collection. The device comprises a base, a moving mechanism arranged at the bottom of the base, a lifting mechanism arranged at the upper surface of the base, a connecting column arranged at the lifting end of the lifting mechanism, a collecting cylinder for collecting corn pollen fixedly connected to the right end of the connecting column, a shaking mechanism arranged at the bottom of the outer surface of the collecting cylinder, a shaking end of the shaking mechanism penetrating into the interior of the collecting cylinder and fixedly connected to a contact plate for contacting with a corn cob, a plurality of inclined guide plates arranged on the inner wall of the collecting cylinder, a plurality of communication openings arranged on the upper surface of each guide plate, and a collecting mechanism connected to all the communication openings through connecting hoses. The contact plate is driven to reciprocally shake the corn cob, so that the corn pollen is accelerated to fall off and the efficiency of corn pollen collection is improved.

The application provides a portable corn field pollen collecting and pollinating device, which comprises cover body one and cover body two, the top of the cover body one and the cover body two is respectively provided with top plate one and top plate two, and the bottom of the cover body one and the cover body two is a semicircular opening; the top of the top plate one and the top plate two is provided with elastic telescopic rods, the two ends of the elastic telescopic rods are respectively fixedly connected with the top plate one and the top plate two; the top plate two is provided with a pneumatic assembly, the pneumatic assembly comprises a cylinder body, the side of the cylinder body is provided with an air inlet and an air outlet, a piston and an elastic rod for the piston are slidably arranged in the cylinder body, a guide rod is arranged on the piston, and a support rod is arranged on the guide rod; a pull rope is arranged between the top plate one and the support rod; the top plate two is provided with a pollen beating assembly; the bottom of the cover body two is provided with a discharge port, the discharge port is connected with the inlet of an air blower, the outlet of the air blower is connected with a pipeline of a collecting device, and the collecting device is connected with a pipeline of a pollinator. The application can efficiently collect corn pollen and perform pollination operation, and the device structure is ingenious and easy to operate.

The application belongs to the technical field of genetic engineering, and particularly relates to application of ZmVAMP726 gene in regulation of pollen stress resistance of plants, wherein the nucleotide sequence of the ZmVAMP726 gene is shown as SEQ ID NO:1. Research finds that deletion of the gene leads to sensitivity of Arabidopsis pollen to high temperature and ultraviolet radiation, and overexpression of the gene improves the resistance of plants to high temperature and ultraviolet radiation. The application provides a new strategy for subsequent identification and cloning of more corn pollen stress resistance related genes, and breeding of corn.

Disclosed is a method of preserving pollen and storing pollen in a diluent that contains between 10% and 50% starch granules by weight or by volume. The preservation technique maintains pollen viability such that the pollen can be used to pollinate plants and create seeds. The method results in stored pollen with improved viability as compared to pollen that is left in ambient conditions or that is mixed with other diluents that do not contain the starch component. The starch can be derived from any source. Also encompassed by the invention are compositions of pollen in diluent mixtures wherein the diluent contains between 10% and 50% starch granules by weight or by volume. The compositions include the separation of dead pollen contents from live pollen grains by using starch mixed with other solid particulates.

The invention belongs to the technical field of plant genetic transformation, and discloses a liposome-mediated maize pollen transfection method. The method comprises the following steps: preparing a plasmid DNA solution and a mixed lipid vesicle SMV solution composed of lecithin and cationic lipid DOTMA, and mixing and incubating the plasmid DNA solution and the mixed lipid vesicle SMV solution according to a mass ratio to form an SMV-DNA compound transfection solution; collecting and pretreating fresh corn pollen, and co-incubating with the transfection solution at low temperature; after drying treatment, the pollen is directly used for pollinating female ears of receptor corn, and transgenic offspring seeds are obtained. According to the method, efficient delivery of exogenous DNA is realized by utilizing affinity and electrostatic interaction of liposome and pollen cell membranes, tissue culture is not needed, the method is independent of genotypes, the method is simple and convenient to operate and low in cost, the problem of somatic cell variation is effectively avoided, the transformation efficiency can reach about 1.4%, and an efficient and practical new technical approach is provided for corn genetic improvement.

The utility model relates to corn pollen collection technical field, concretely is corn breeding pollen collection device with automatic cleaning function, including back box and collection cylinder, the back surface fixed connection of back box has two symmetrical braces, the right side surface fixed mounting of back box has the fan, the suction end of fan extends to the inside of back box and is equipped with filter bag. The device in the use, the corn ear is put in between two transmission rods, at this moment, the operation of micro motor drives the rotation of reciprocating screw rod, and the threaded block drives two transmission rods to carry out the back and forth reciprocating movement through the rotation of reciprocating screw rod, and the pollen is shaken off, and at the same time, the operation of fan makes the negative pressure in the inside of back box, and the pollen in the collection cylinder is sucked into the inside of back box through the hose, during, through the setting of screen board, can carry out the filtration to petal, broken leaf, to improve the purity of pollen collection in the inside of back box, provide the convenience for the collection work of pollen, alleviate the work burden of staff, and the collection effect is good.

The application discloses a corn receptor kinase-encoding gene ZmER2, wherein the CDS sequence of the gene is shown as SEQ ID No.1.The application also discloses corn mutants containing the gene ZmER2, which are named mutant Zmer2-D17 and mutant Zmer2-I1 respectively, wherein the CDS nucleotide sequence of the mutant Zmer2-D17 is shown as SEQ ID No.3, and the amino acid sequence of the gene is shown as SEQ ID No.4; the CDS nucleotide sequence of the mutant Zmer2-I1 is shown as SEQ ID No.5, and the amino acid sequence of the gene is shown as SEQ ID No.6.The application also discloses that after the gene ZmER2 is mutated, a male spike non-powdering phenotype appears without affecting plant height, ear position height and ear row number and other yield characters, the phenotype is valuable material for studying pollen development, cytoplasmic inheritance and nucleo-cytoplasmic interaction, and indicates that the ZmER2 gene has a good application prospect in hybrid seed production.

The utility model relates to the technical field of corn breeding assistance, in particular to a corn seed reproduction breeding pollination device which comprises a bearing plate, a screening hopper and a bottom shell, four supporting legs are arranged at the bottom of the bearing plate, four guide rods are arranged in the screening hopper, two screening plates are arranged on the four guide rods in a sliding mode, a shaking mechanism is arranged in the screening hopper, and the bottom shell is arranged on the bottom shell. Discharging windows are formed in the two sides of the screening hopper respectively, sealing pieces are arranged in the discharging windows, a powder discharging pipe is arranged at the bottom of the screening hopper, an adjusting mechanism is arranged on the powder discharging pipe, an externally-lapped protrusion is arranged on the outer wall of the screening hopper, the bottom shell is arranged at the top of the bearing plate, and the externally-lapped protrusion is fixedly connected to the top of the bottom shell. According to the corn pollen screening device, the discharging windows are formed in the two sides of the screening hopper, corn pollen remaining on the screening plates can be cleaned by opening the discharging windows through the sealing pieces, the two screening plates can be shaken through the shaking mechanism, screening holes of the screening plates are prevented from being blocked by the corn pollen, and the screening quality of the corn pollen is improved.

The invention belongs to the field of gene engineering, and discloses a maize pollen fertility regulation gene ZmPUB33 and an encoding protein thereof. The gene mediates the fertility of pollen by regulating and controlling the development of corn pollen pores, the gene cannot bloom normally after mutation, anther glumes cannot be cracked basically, anther is smaller than a wild type and is almost not exposed, pollen grains are inactive, complete male sterility of plants can be caused, the sterile line can be used for sterile seed production and production of crop hybrids, and the application prospect is wide. And the method has huge application and economic values.

The invention relates to the technical field of corn breeding, and discloses a seed production device suitable for corn seed production, the seed production device comprises a lifting frame, a sleeve is fixedly connected in the lifting frame, an exhaust fan is fixedly arranged on the upper surface of the sleeve, and a collecting cylinder is mounted on the side, away from the sleeve, of the exhaust fan; the outer wall of the collecting barrel is installed in the lifting frame, a plurality of supporting rods are fixedly connected to the outer wall of the lower side of the sleeve, a plurality of bottom plates are slidably connected to the outer walls of the supporting rods, the upper surfaces of the bottom plates are attached to the lower surface of the sleeve, and electric push rods are installed in the bottom plates. Through mutual cooperation between the first air bag and the expansion block, when male parent corn pollen is automatically collected, different corn rhizomes are automatically clamped, meanwhile, the interior of the sleeve can be sealed, and the effects that the pollen utilization rate is reduced and the body health of operators is affected due to pollen overflowing are avoided.

The pollen collecting device comprises a collecting cover with an opening in the lower end, a cavity of an inverted-U-shaped structure is formed in the inner wall of the collecting cover and composed of a vertical cavity I, a transverse cavity and a vertical cavity II, the vertical cavity I communicates with the vertical cavity II through the transverse cavity, and an air opening I communicating with the interior of the collecting cover is formed in the side wall of the vertical cavity I; a filter screen for intercepting pollen is arranged in the vertical cavity I, a collecting bottle is detachably connected to the lower end of the vertical cavity I through a vertical pipe, an air opening II communicated with the interior of the collecting cover is formed in the side wall of the vertical cavity II, and an air blowing structure is arranged at the air opening II. When corn pollen is collected, tassels do not need to be bent, the tassels can be directly sleeved with the collecting cover only by lifting the vertical pipe with a hand, pollen collecting work can be conducted, operation is convenient and fast, the pollen collecting speed is high, and the problem that the tassels are broken can be avoided. In addition, after the vertical pipe is lifted by hand, the tassels can be directly sleeved with the collecting cover conveniently.

The invention relates to the technical field of cosmetics, in particular to a moisturizing and barrier repairing pollen composition and a preparation method and application thereof. The raw materials of the pollen composition for moisturizing and repairing the barrier comprise the pine pollen, the corn pollen, the lotus pollen and the rape pollen, a synergistic effect is achieved through the specific pollen composition, the moisturizing and barrier repairing effects are remarkably improved, the sensitization is reduced, and the pollen composition is safer and more efficient.

The invention discloses a corn pollen grain identification and perimeter and area calculation method based on DianView software, and belongs to the technical field of crop germplasm resource identification and breeding. The method comprises the following steps: firstly collecting pollen in the middle of a corn tassel, storing at low temperature, imaging under a microscope after I2-KI dyeing, then importing an image into DianView software, utilizing a watershed algorithm and combining with artificial interaction correction, accurately segmenting adhered pollen and removing impurities, and finally measuring and exporting data such as long and short axes, perimeter and area of the pollen. According to the method, accurate quantification and digital tracing of micron-sized pollen characteristics are realized through standardized process and software assistance, and reliable technical support is provided for improvement of ideal plant types and sufficient pollen of corn and parent screening.

The invention discloses a novel Chinese yeast and a preparation method thereof. The Chinese yeast is prepared from rice flour, water and one or more of the following plants: green seedlings of grains, ears of grains in a milk-ripe stage, flowers of edible flowers, edible green-leaf plants, medicinal and edible plants taking leaves as medicines, medicinal plants which take leaves as medicines and can be used for health foods, pine pollen and bee pollen. Such as wheat seedlings, corn seedlings, wheat ears, alfalfa, kale, peanut leaves, grape leaves, rosemary, mint, motherwort, pine pollen, corn pollen, linden pollen, roses, sweet-scented osmanthus and the like; plants in the Chinese yeast are selected from food, medicinal and edible homologous and health-care food plants, so that the Chinese yeast has very reliable edible safety and health-care functions, and the plants are wide in source, easy to plant and convenient to harvest; the preparation process of the Chinese yeast is simple, short in period and low in labor intensity; the prepared Chinese yeast has good saccharification and fermentation capabilities, and can be used for brewing Chinese rice wine, rice wine, yellow wine, beer and white spirit.

The invention discloses a plant pollen specific expression promoter POsPTDb and application thereof. The nucleotide of the promoter POsPTDb is as shown in SEQ ID NO. 1; the pollen specific promoter POsPTDb is derived from a promoter of a rice endogenous gene, and can specifically drive a target gene or a DNA fragment to be expressed in pollen; the promoter is used for driving specific expression of a gene or a DNA (deoxyribonucleic acid) fragment capable of controlling pollen fertility in pollen, gametophyte male sterility can be prepared, and the promoter has important value in the aspects of preventing transgenosis from drifting along with pollen, breeding sporophyte nuclear sterility and the like.

The invention relates to the technical field of food preservation, in particular to a low-temperature vacuum dehydration and preservation process for fresh corn. Comprising the following steps: draining fresh corns, and soaking the drained fresh corns in a fresh corn preservative for 10-15 minutes; the fresh corn preservative is prepared from the following components in parts by weight: 5.5 to 8.2 parts of corn pollen polysaccharide, 2 to 5 parts of sodium diacetate, 1.5 to 2.5 parts of lysozyme, 4 to 12 parts of chitosan, 2 to 4 parts of tea polyphenol, 6 to 18 parts of sorbitol, 2 to 4 parts of potassium sorbate, 0.6 to 1.2 parts of span-80, 2.2 to 5.6 parts of tween-80 and 10 to 15 parts of edible soybean oil. And taking out, draining, and storing at 0-4 DEG C. The invention provides a biological fresh-keeping process for fresh corn, which is simple in method, low in cost and good in fresh-keeping effect.