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Enzymolysis method of pollen

A technology of enzymatic hydrolysis and pollen, applied in the field of enzymatic hydrolysis, can solve the problems of insufficient absorption and utilization, difficult absorption and utilization, low yield of extract, etc., and achieve the effect of being suitable for popularization, complete enzymolysis, and increasing yield of extract

Active Publication Date: 2019-03-08
HUNAN DEHAI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Pollen (pine pollen, corn pollen, rape pollen) is a very nutritious natural resource, but due to the dense pollen wall, its nutrients are contained in the pollen wall , not easy to dissociate for human body absorption and utilization, direct use or insufficient wall breaking, such as mechanical wall breaking, will make its absorption and utilization insufficient, and the direct non-enzymatic technology (direct water extraction) yield is 5%, and its extract The yield is extremely low, so we designed a pollen enzymatic hydrolysis method to solve the above problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for enzymatic hydrolysis of pollen, comprising the following steps:

[0024] S1. Enzymolysis: Take 5Kg of pine pollen, 10Kg of corn pollen, 5kg of rapeseed pollen, add 100L reaction kettle after breaking the wall, add 80Kg of purified water, 100g of cellulase, 100g of α-amylase, heat up to 40°C±5°C, React for 5h±0.5h; heat to 80°C, then add 100g of protease, and react for 12h;

[0025] S2, filtration: after the reaction is completed, filter while it is hot, and get the filtrate;

[0026] S3. Concentration: Concentrate under reduced pressure to a density of about 1.1, and release the extract;

[0027] S4. Spray drying: open the steam valve to make the steam pressure to 0.1MPa, and keep the temperature in the spray drying tank at 110°C-120°C; open the compressed air valve and turn on the peristaltic pump at the same time to spray the liquid medicine into the tank in the form of mist. Collect dry powder;

[0028] S5. Mixing and granulation: Mix the extract powd...

Embodiment 2

[0032] S1. Enzymolysis: Take 5Kg of pine pollen, 10Kg of corn pollen, 5kg of rapeseed pollen, add 100L reaction kettle after breaking the wall, add 80Kg of purified water, 100g of α-amylase, heat up to 40℃±5℃, react for 5h±0.5h ;

[0033] S2, filtration: after the reaction is completed, filter while it is hot, and get the filtrate;

[0034] S3. Concentration: Concentrate under reduced pressure to a density of about 1.1, and release the extract;

[0035] S4. Spray drying: open the steam valve to make the steam pressure to 0.1MPa, and keep the temperature in the spray drying tank at 110°C-120°C; open the compressed air valve and turn on the peristaltic pump at the same time to spray the liquid medicine into the tank in the form of mist. Collect dry powder;

[0036] S5. Mixing and granulation: Mix the extract powder: β-cyclodextrin: purified water at a ratio of 1:1:0.1 for 20 minutes, mix evenly, perform oscillating granulation in the oscillating granulator, and pass through a ...

Embodiment 3

[0040] S1. Enzymolysis: Take 5Kg of pine pollen, 10Kg of corn pollen, 5kg of rapeseed pollen, add 100L reaction kettle after breaking the wall, add 80Kg of purified water, 100g of α-amylase, 100g of cellulase, heat up to 40°C±5°C, Reaction 5h±0.5h;

[0041] S2, filtration: after the reaction is completed, filter while it is hot, and get the filtrate;

[0042] S3. Concentration: Concentrate under reduced pressure to a density of about 1.1, and release the extract;

[0043] S4. Spray drying: open the steam valve to make the steam pressure to 0.1MPa, and keep the temperature in the spray drying tank at 110°C-120°C; open the compressed air valve and turn on the peristaltic pump at the same time to spray the liquid medicine into the tank in the form of mist. Collect dry powder;

[0044] S5. Mixing and granulation: Mix the extract powder: β-cyclodextrin: purified water at a ratio of 1:1:0.1 for 20 minutes, mix evenly, perform oscillating granulation in the oscillating granulator, ...

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Abstract

The invention discloses an enzymolysis method of pollen. The method comprises the following steps: S1, enzymolysis: taking 5Kg of pine pollen, 10Kg of corn pollen and 5Kg of rape pollen, performing wall breaking, adding the obtained pollen into a 100L reaction kettle, adding 80Kg of purified water, 100g of cellulase and 100g of alpha-cellulose into the reaction kettle, heating the mixture in the kettle to 40+ / -5 DEG C, performing reaction for 5+ / -0.5 hours, heating the obtained material to 80 DEG C, then adding 100g of protease, and performing reaction for 12 hours; S2, filtration: performingfiltration while the obtained mixture is hot after the reaction is finished, and taking filtrate; and S3, concentration: performing concentration under reduced pressure until the density is about 1.1,and discharging extractum. The method is simple and safe in process, and does not use other organic reagent compared with traditional enzymolysis water extraction. Besides, the enzymolysis of the invention is full, and meanwhile, the content of micromolecular peptides after enzymolysis is 30% or above. The method greatly improves the absorption effect for the human body, and is suitable for popularization and use.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis method, in particular to an enzymatic hydrolysis method of pollen. Background technique [0002] Pollen (pine pollen, corn pollen, rape pollen) is a very nutritious natural resource, but due to the dense pollen wall, its nutrients are contained in the pollen wall, which is not easy to be free for human body to absorb and utilize, and it is not enough to use directly or break the wall , such as mechanical breaking, will make its absorption and utilization insufficient, and the yield of non-enzymatic hydrolysis technology (direct water extraction) is 5%, and the yield of its extract is extremely low. Therefore, we have designed a pollen enzyme solution to solve the above problems. Contents of the invention [0003] The purpose of the present invention is to solve the shortcomings in the prior art, and propose a pollen enzymatic hydrolysis method. [0004] In order to achieve the above object, the prese...

Claims

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Application Information

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IPC IPC(8): A23L21/20
CPCA23L21/20
Inventor 杨银芝王刚黄广菊田清华
Owner HUNAN DEHAI PHARM CO LTD
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