344results about How to "Reduce vitality" patented technology

Topical use of teprenone in a cosmetic or dermopharmaceutical composition as an active preventing and / or limiting the skin cells sénescence and / or protecting the general state of the skin.

The invention relates to a seedling growing method of apocarya and belongs to the technical field of fruit trees and forest cultivation. The method includes the steps of sprouting breeding, grafting and post-grafting management. By means of the method, a large amount of scions are obtained each year and are grafted on the stock and the seedling with strong affinity in due time, so that stock grafting survival rate of the apocarya is improved. Grafted seedling preserving rate after large-amount grafting is over 90%. Further, the method achieves tree body stuntedness, shortens the juvenile phase and enables tree bodies to fruit earlier in the fourth year after grafting. The fruiting time is 6-8 years earlier than before. Simultaneously, the method provides a growing method for apocarya stock grafting seedlings and with strong operability for batch and variety seedling growing of the apocarya and greatly increases economical value of the apocarya.

The invention relates to the field of culture of tumor organoids, and in particular relates to a special medium used for in-vitro culture of esophagus cancer tumor organoid based on human esophagus cancer tissue and a culture method. The special medium comprises a receptor tyrosine kinase ligand, an Rho-associated coiled-coil protein kinase inhibitor, vitamin and hormone, an antioxidant, and an agonist for inhibiting cell differentiation pathway. According to the method, through the step-by-step culture technology adopting conditional dedifferentiation, tumor cells rapidly amplify within a short time to form the organoid. Through the step-by-step culture technology, primary cells rapidly form the organoid, meanwhile, adequate cells are formed within the effective time, so that the follow-up experiment operation can be conveniently carried out, and therefore, the culture speed and success rate of the organoid are improved.

A health care drink is prepared by combining 22 medicinal herbs and supplementing vitamin, amino acid, food raw materials and a food additive. The health care drink can apply 'Jian' permit, and has the functions of alleviating physical fatigue and asthenopia and strengthening human immunity. The health care drink has wide applicable population, broad market development space and fills up a blank of the health care products.

The invention relates to a method for alive preservation and transportation of sea cucumber, which belongs to the technical field of aquatic products. The method comprises the following steps: holding the transported sea cucumber (naturally fished and caught or artificially cultured) for at least two days, transferring the sea cucumber with strong vitality after holding into a cooling barrel which is disinfected in advance and contains filtered sea water for performing cooling treatment, then placing into a plastic bag (a small sponge after being soaked in the sea water at the temperature of 20 DEG C is arranged in the bag), exhausting the air in the bag, enabling the bag to be rapidly filled with oxygen, binding up a bag mouth, and boxing; loading two layers or three layers or four layers in each heat insulation box, determining according to the size of the sea cucumber and the size of space in each box, separating the layers by hardboards, using foam boards to support at the middle parts, preventing the plastic bag from being damaged by extrusion and further avoiding death caused by hypoxia of the sea cucumber or oppression on the sea cucumber; placing the upper layer of the hardboard into a bag which loads ice blocks in advance or a heat insulation bottle for heat insulation; and sealing with an adhesive tape after well boxing, marking and starting the transportation as soon as possible. By adopting the method, the survival rate of the sea cucumber during the transportation is effectively improved, and the transportation cost is reduced.

The invention discloses a method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal, and relates to the technical field of farm products bioprocess. A stain of high-yield protease bacillus subtilis which is sorted from conventional Jiangxi fermented food is used for being matched with brewing yeast and lactobacillus plantarum to carry out the mixed solid fermentation on high-temperature soybean meal, slant activation and liquid enlarge cultivation are carried out, aerobic fermentation is carried out for single inoculation, anaerobic fermentation is carried out for the mixed inoculation, then the high-temperature soybean meal is dried at the temperature of 60 DEG C to obtain a fermented soybean meal feed product in which with protein nutrients are rich, antinutritional factors are passivated almost, the palatability is improved and the bioactivity is high. The method has the advantages that the constitution of the protein and the antinutritional factors of the high-temperature soybean meal fermented product is further improved, the palatability of the fermented product is improved, and the fermented product has the characteristics of rich nutrients, low resistance and palatability.

The present invention relates to face-beautifying preparation with the functions of removing facial chloasma, whitening facial skin and removing facial wrinkles. Said face-beautifying preparation contains 6 ingredients of rose extract, Chinese angelica root extract, carthamus flower extract, grape seed extract, vitamin C and vitamin E. Besides, said invention also provides its preparation method.

The invention relates to a biologically-aided totally-chlorine-free bleaching technology of flax cloth or fiber. An oxygen-contained bleaching agent generally functions on lignin far less than a chloride-contained bleaching agent, so developing a novel pollution-free method for removing lignin in cloth fibers is necessary to realize chlorine-free bleaching; the removal of the lignin is the key of effective bleaching especially for fibers such as the flax fibers containing more lignin. The biologically-aided totally-chlorine-free bleaching technology of flax cloth or fiber comprises the following steps: flax raw cloth is subjected to the alkali boiling off and acid pickling, then is subjected to the biological pretreatment and bleached by hydrogen peroxide; and the hydrogen peroxide re-bleaching process is carried out at the following conditions: 4-15g / L hydrogen peroxide, the pH value of 7-11, the bleaching temperature of 40-80 DEG C, the bath ratio of 1:5-20, and the bleaching time of 0.5-2 hours; and the whiteness of products after bleaching can reach up to more than 80 percent, the tensile strength can reach up to 500-900*800-1200N, the capillary effect is more than 8 cm, and the prior requirements of the bleaching technical indications of flax cloth are met. The invention is used for the treatment of flax cloth or fibers under the chlorine-free condition.

The invention discloses a method for increasing yield of hermetia illucens by using kitchen waste and chicken manure. The method comprises the following steps: (1) collecting fresh kitchen waste, andpicking up inorganic impurities in the kitchen waste, then, filtering out extra water by a filter net, and preparing kitchen waste pulp with moisture content of 70%-85% by a pulping machine; (2) collecting fresh kitchen manure, uniformly mixing the kitchen waste pulp and the collected fresh chicken manure in a certain proportion to obtain a mixture, so that the dry weight content of carbohydrate in the mixture is 28%-40%, the dry weight content of protein is 15%-25% and the moisture content is 65%-80%; (3) laying the mixture in a hermetia illucens culture tank, then, putting hermetia illucenslarvae at 2-3 day age into the hermetia illucens culture tank, controlling the temperature at 29-37 DEG C, and carrying out treatment for 7-13 days; and (4) before the hermetia illucens larvae enter aprepupa stage, separating the hermetia illucens from hermetia illucens dung. The method can greatly reduce the feed ratio of the mixture obtained by mixing the kitchen waste pulp and the fresh chicken manure, and greatly increase the larva producing rate of the hermetia illucens.

The invention discloses a production method of a low-salt oyster polypeptide and oligosaccharide nutrition powder. According to the production method, oyster obtained via shell removing is taken as a raw material, is repeatedly immersed in ice water at 4 to 10 DEG C for 2 to 4h; the oyster obtained via immersion desalting is subjected to slurry homogenizing, and is subjected to high temperature treatment for disinfection and protein denaturation; obtained oyster slurry is cooled with normal temperature water; pH value is adjusted to be 7.0 to 9.0, and 0.5 to 1.0wt% of protease is added for protein enzymatic hydrolysis; after high pH protease enzymatic hydrolysis, the temperature is adjusted, and 0.5 to 1% of a weakly acidic protease is added for enzymatic hydrolysis; amylase is added for degradation of glycogen; an obtained product is subjected to high temperature treatment, filtering, and spray drying so as to obtain the oyster polypeptide and oligosaccharide nutrition powder. The production method is simple; cost is low; and the obtained oyster polypeptide and oligosaccharide nutrition powder can be absorbed by human body easily.

A soybean protein in the form of gel is prepared from the low-temp deoiled soybean dregs through heating for deactivating lipoxidase to obtain a suspension, regulating temp and pH value, alkali dissolving, acid deposition and separating gel from liquid.

The invention discloses a haze care mask which is formed by adding four magnetic sheets in the conventional mask consisting of multiple fabrics (3), wherein the two magnetic sheets (4) are arranged on the two sides of a nose wing and correspond to Yingxiang acupunctures, and the other two magnetic sheets (2) are arranged on the outer sides of the lower parts of the two magnetic sheets (4) and correspond to Xiaguan acupunctures. As the mask is provided with the magnetic sheets to generate a magnetic field, primary and secondary particles and metal particles with negative and positive charges in haze deflect to be far away from an inhalation channel under the action of Lorentz force; and the mask also has the filtering function of the conventional mask. Therefore, under the two actions, inhaled harmful substances are greatly reduced, and the damage on the health of people is reduced when the people go out and work in conditions with serious pollution. As the mask is provided with the magnetic sheets corresponding to the acupunctures of a human body, the mask plays a role of health care when being worn for a long time.

The invention discloses a nutrient solution for arranged flowers. The nutrient solution comprises the raw materials in percentage by weight: 0.8-1.2% of saccharose, 0.7-1.4% of glucose, 0.08-0.105% of edible salt, 0.025-0.035% of aspirin, 0.1-0.14% of vitamin C, and the balance of water. Compared with the prior art, the nutrient solution has the following advantages that nutrient components required by arranged flowers are supplied to guarantee normal metabolism of the arranged flowers; the breath speed of the arranged flowers is slowed down, and the aging and withering of the arranged flowers is reduced; and because the activity of microorganism is blocked, the phenomenon of untimely rotting and decaying of the arranged flowers is avoided. By using the nutrient solution for the arranged flowers, the freshness retaining time of the arranged flowers can keep for 20-30 days, and the color is bright and the luster is lovely.

The present invention discloses the use of a protease inhibitor in the preparation of a medicament for the treatment of microbial infections. The invention further discloses the use of a compound binding to a cross-β structure or an antibody specific for a cross-β structure in the preparation of a medicament for the treatment of microbial infections. The invention further discloses methods for attenuating microbial pathogens by deleting at least part of a gene encoding a cross-β structure forming protein. The invention also discloses an antimicrobial composition, and a kit for detecting microbial contamination in a solution or a substance.

A novel squash inbred line, designated KAZA121, is disclosed. The invention relates to the seeds of squash inbred line KAZA121, to the plants of squash inbred line KAZA121 and to methods for producing a squash plant by crossing the squash inbred line KAZA121 with itself or another squash line. The invention further relates to methods for producing a squash plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other squash lines derived from the squash inbred line KAZA121.

The invention relates to a Chinese medicinal herb feed additive for regulating the fat metabolism of a Chinese softshell turtle, promoting growth and enhancing immunity and a feed containing the same. The Chinese medicinal herb feed additive is prepared from cassia seeds, fructus hordei germinatus, hawthorns, lotus leaves, medicated leaven and radix paeoniae alba by the steps of drying, crushing and uniformly mixing. After being added to the common feed of the Chinese softshell turtle, the Chinese medicinal herb feed additive disclosed by the invention can outstandingly increase the growth speed of the Chinese softshell turtle, promote the fat metabolism of an organism and enhance the nonspecific immunity capacity of the Chinese softshell turtle, outstandingly enhance the T-SOD (Total Superoxide Dismutase) and LSZ (Lysozyme) activity of serums and outstandingly reduce the LDH (Lactate Dehydrogenase) activity and the content of MDA (Methane Dicarboxylic Aldehyde), TG (Triglyceride), TC (Total Cholesterol) and LDL-C (Low-Density Lipoprotein Cholesterol). The Chinese medicinal herb feed additive is environment-friendly, natural, does not generate drug fast or residues, has multiple functions as well as characteristics such as growth consideration and fatty liver resistance, can greatly improve the survival rate of the Chinese softshell turtle and reduce the culture cost of the Chinese softshell turtle and is suitable for industrialized production.

The invention relates to separation, purification and primary culture methods of intestinal macrophages in Ctenopharyngodon idella. The separation method comprises: taking out the rear middle segment of an intestine by aseptic operation, removing intestinal outer fat and mesentery, longitudinally cutting the intestine to clear excrement, and using aseptic bent-tip tweezers to scrape off the intestinal mucus and epithelial cell layer for 15 min; cutting the segment with the mucus and epithelial cell layer removed to obtain fragments, and digesting the fragments in collagenase IV digestive juice to obtain lamina propria single-cell suspension; using a separation kit of fish organ monocytes to separate the intestinal macrophages. The purification method comprises: using a differential wall attachment method to purify the intestinal macrophages. The primary culture method comprises: using RPMI (Roswell Park Memorial Institute medium) 1640 complete culture solution containing autoserum of Ctenopharyngodon idella to culture the intestinal macrophages, changing the solution once with lipopolysaccharide-containing RPMI 1640 complete culture solution after cells attach to the wall, and changing the solution once every other two days so that cells may survive for at least 20 days. The separation, purification and primary culture methods established herein have good operability and repeatability.

The invention relates to the field of tea preparation, in particular to a preparation method of selenium-rich white tea and the selenium-rich white tea prepared with the method. The method comprises steps as follows: the growth temperature of a tea tree is controlled in the range of 25-35 DEG C about 18-22 days earlier than tea picking, and an organic selenium solution is sprayed on tea buds for each ten days; the tea is fermented for 3-4 hours under the condition of relative humidity of 70%-80%, and fermented tea is obtained; and the fermented tea is dried under the condition of relative humidity of 70%-80% at the temperature of 24 DEG C-28 DEG C until the water content is 6%-10%, and the selenium-rich white tea is obtained. The selenium-rich white tea prepared with the preparation method can clear excessive oxygen radical in a human body, lipid peroxidation damage of a cell membrane is prevented, and the anti-free radical action of the selenium-rich white tea is 300-500 times of vitamin E and vitamin C.

The invention discloses citrus steamed fish sauce and a preparation method thereof, and belongs to the technical field of food fermentation engineering. According to the citrus steamed fish sauce disclosed by the invention, the technology that soybeans, flour, bran, citrus peels and purple perilla are used as starter propagation raw materials and aspergillus oryzae 3.042 and aspergillus niger 3.350 cooperate for starter propagation is adopted, at the middle stage of fermenting soy sauce mash, saccharomyces rouxii is added, and at the later stage, torulopsis bombicola is added. The aspergillus niger has higher cellulose activity, lipase activity and pectinase activity, and can achieve the enzyme system complementary effect with the aspergillus oryzae 3.042. The activity of the cellulase, the activity of the lipase and the activity of the pectinase obtained at the stage of starter propagation are higher, the cellulase, the lipase and the pectinase are used for decomposing the citrus peels and the purple perilla and promoting the release of various active substances in the citrus skins and the purple perilla. The addition of the two kinds of salt resistant fragrance-producing yeasts at the middle stage and the later stage of fermentation can promote the formation of flavor substances of the steamed fish sauce, improve the flavor of the steamed fish sauce and increase the kinds of the flavor substances.

A dust-removing disinfecting unit for centralized air conditioner system is connected in the ventilation channel, and is composed of (80-150)-mesh dust-filtering net, several disinfecting nets, ozone-removing net, and ultraviolet lamp. Its advantage is high effect.

The invention provides a method for grafting Ilex sp. by taking Ilex dabieshanensis as a stock. The method comprises the following steps: performing ingraftment scion on the Ilex dabieshanensis serving as the stock and an Ilex sp. new variety serving as a scion by means of a cut grafting method; and performing layered alignment on the Ilex dabieshanensis and the Ilex sp. to graft successfully. Through adoption of the method, the adaptability of the Ilex sp. new variety is improved, the stock is readily-available, the cost is low, the propagation coefficient is increased, the yielding capacity is increased, and scale production of the Ilex sp. new variety becomes possible.

The invention relates to an application of Trichoderma pseudokoningii extracellular polysaccharides having anticancer activities. The Trichoderma pseudokoningii extracellular polysaccharides having the anticancer activities can reduce the vitality of K562 cells in vitro when they are applied to tumor growth inhibition (auxiliary) medicines, so intracellular active oxygen and free calcium ion concentrations are improved, the expression abundance of mRNA of p53 and Bax genes is improved, the expression of the Bc1-2 transcription level is reduced, the ectropion of cell membrane phosphatidylserine of the human chronic granulocyte leukemia cells K562 is induced, and the nuclear polycondensation and the fragmentation of K562 are induced to generate apoptotic bodies and apoptosis; and in-vivo administration can increase weights of tumor bearing mice and alleviate growth speeds and weights of tumors of the tumor bearing mice, thereby tumor cell killing and tumor growth inhibiting purposes are reached.

The invention provides a cucumber CsGL2-LIKE gene and application thereof in regulating the partial abortion of male flowers. The nucleotide sequence of the cucumber CsGL2-LIKE gene is shown as SEQ IDNO. 2, and the amino acid sequence of an encoded protein of the gene is shown as SEQ ID NO. 1. An interference vector of the CsGL2-LIKE gene is constructed and then transformed into cucumber by an agrobacterium-mediated transformation technology; it is proved that the down-regulation of the CsGL2-LIKE gene causes the change of the shape and pollen viability of cucumber stamens, resulting in the partial abortion of the male flowers of transgenic plants, the decline of pollen viability and the weakening of the elongation ability of pollen tubes; however, female flowers are not affected, it is shown that the function of the CsGL2-LIKE gene is related to the partial abortion of the cucumber male flowers, and it is indicated that the gene can have an important improvement potential in cucumberbreeding and quality improvement.

An effect adding method, includes: applying different gains to a positive side waveform portion and a negative side waveform portion of an audio signal respectively when absolute values of input levels of the positive side waveform portion and the negative side waveform portion are smaller than a predetermined value; producing a higher range component of the audio signal based on a high range component of the audio signal to which the gain is applied, the higher range component being higher in frequency than the high range component; producing a lower range component of the audio signal based on a low range component of the audio signal to which the gain is applied, the lower range component being lower in the frequency than the low range component; and synthesizing an audio signal having an effect sound by adding the audio signal to which the different gains are applied, the higher range component, and the lower range component with each other.

The invention discloses the enzyme-reaction low-temperature current goods shelves life indicating device, which comprises the transparent container that has the plastic diaphragm in order to divide the container into non-communicated chamber one and two. The chamber one is filled with urea enzyme and the chamber two with the base material and the pH indicator.

The invention discloses alkaline thermal-stability SGNH family esterase EstD1. The amino acid sequence of the alkaline thermal-stability SGNH family esterase EstD1 is one of the amino acid sequence (1) shown in SEQ ID NO.1 and the amino acid sequence (2), wherein one or more pieces of amino acid are added or replaced or lacked or inserted, shown in SEQ ID NO.1. The invention further discloses a gene EstD1 of the alkaline thermal-stability SGNH family esterase EstD1, the nucleotide sequence of the gene EstD1 is one of the nucleotide sequence (1) shown in SEQ ID NO.2 and the nucleotide sequence (2), wherein one or more pieces of nucleotide are added, replaced, lacked or inserted, shown in SEQ ID NO.2, the nucleotide sequence (3) formed through hybridization with the nucleotide sequence (1) or nucleotide sequence (2) under strict conditions and the nucleotide sequence (4) which is different from the nucleotide sequence (1), the nucleotide sequence (2) and the nucleotide sequence (3) due to the degeneracy of a genetic codon. The alkaline thermal-stability SGNH family esterase EstD1 can be used for deacetylation of 7-ACA.

The invention discloses heat-resistant nitrile hydratase, engineering bacteria and application thereof in production of amide by catalyzing hydration reaction of nitrile compounds. The heat-resistantnitrile hydratase contains alpha-subunit coding genes with a base sequence shown as SEQ ID NO.4, beta-subunit coding genes with a base sequence shown as SEQ ID NO.5, and active element coding genes with a base sequence shown as SEQ ID NO.6. The nitrile hydratase genes are cloned from aurantimonas manganoxydans SI859A and successfully subjected to heterologous expression in E. coli BL21 (DE3), so as to obtain nitrile hydratase with high expression quantity and high activity and proteins with specific activities reaching 404.5U / mg. The nitrile hydratase has high catalytic activity on aromatic nitrile compounds and aliphatic nitrile compounds, and is a kind of nitrile hydratase with wide substrate spectrum, high thermal stability and excellent industrial application potential.

The invention provides a method for quickly breaking dormancy of birch-leaf pear seeds with hydroxylamine chloride, which relates to the breaking of seed dormancy and seed germination and belongs to the technical field of plant production. The method comprises the following steps: peeling off pericarp of a ripening fruit; rubbing the peeled fruit to eliminate pulp; soaking seeds in sodium hypochlorite (NaClO) of which the mass volume ratio is 20 percent to remove the pulp adhered to seed capsules; sterilizing the seeds by soaking the seeds in a mercuric chloride solution of which the mass volume ratio is 0.1 percent for 8 minutes; taking the cleaned birch-leaf pear seeds, and presoaking the seeds for 24 hours respectively in the hydroxylamine chloride of which the mass volume ratio is 0.8 percent at room temperature; and sowing the seeds after washing the seeds for 5 to 6 times. The main basis of the method is that the birch-leaf pear seeds have the characteristic of embryo dormancy, the dormancy of the birch-leaf pear seeds can be broken after the treatment with the hydroxylamine chloride, and the method can promote the seed germination, increase the seed germination rate, and has tidy germination.

The invention provides a hypothermic anesthesia transportation method for live Oncorhynchus mykiss, the method comprising following steps: 1) feed deprivation: food provisioning for Oncorhynchus mykiss is stopped for 2-3 days before transportation; 2) cooling and anesthesia treatment: water tanks are filled with cooled aquaculture water of 1-2 DEG C; the water temperature is maintained; anesthetic is added to the water and mixed well. The Oncorhynchus mykiss deprived of food is weighed and then put in the aquaculture water and quick cooling and anesthesia are performed. The Oncorhynchus mykiss is taken out of water surface when the activity ability of the fish is nearly exhausted. By this time, the fish bodies are in the state of hypothermic anesthesia and then they are to be encased. 3) the Oncorhynchus mykiss are transported by packing transportation or water track transportation. After arriving at a destination, The Oncorhynchus mykiss are brought back to life and the temperature of aquaculture water is gradually increased to normal level. The hypothermic anesthesia transportation method has following beneficial effects: no matter how much the transportation volume of live fish is, the transportation method can be performed with low cost, simple, flexible and easy operation; and the economic benefit can be guaranteed by high rate of survival of fish after transportation.