Method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal

A production method and soybean meal technology, applied in animal feed, animal feed, application and other directions, can solve the problems of low crude protein content, low palatability and high cost, and achieve the improvement of animal growth performance, improvement of nutritional characteristics, Significant economic effect

Active Publication Date: 2014-01-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many improvements have been made to the process of fermenting soybean meal. For example, the Chinese patent application number 200810219198.7 uses three strains of Bacillus subtilis, Saccharomyces cerevisiae and lactic acid bacteria to ferment soybean meal, which degrades the protein in soybean meal to a certain extent and improves its nutrition. value, but the increase in crude protein content is not high
Liu Xiaoyan and others used Bacillus subtilis, Aspergillus oryzae and Saccharomyces cerevisiae to ferment high-temperature soybean meal, so that the polypeptide yield in the final fermentation product reached 54.89%, and the polypeptide content in the fermentation product was 21.47% (dry basis), which was 4.1 times higher than that of the raw material. Better nutritional value, but the inoculation amount of this method is 25%, the fermentation raw materials need to add bran and molasses to adjust the carbon-nitrogen ratio of the medium, the cost is relatively high, although the flavor of the fermented soybean meal product has improved, it does not have a sour flavor , acidification treatment is still required before feeding, and there is still room for improvement in palatability (Liu Xiaoyan, Yang Guoli, Guo Lidong. Research on the production of soybean polypeptide feed by mixed bacteria solid-state fermentation [J]. Feed Industry, 2012.33(6): 51- 56)
In general, the current high-temperature soybean meal fermentation process still has problems such as high cost, low nutritional value of the product, and low palatability.

Method used

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  • Method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal

Examples

Experimental program
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Effect test

Embodiment 1

[0011] Example 1: The following technical scheme is adopted in the example of this specific embodiment: a method for making a peptide-rich prebiotic feed produced by fermenting high-temperature soybean meal. Take one ring from Lactobacillus plantarum and put them on beef extract peptone slant medium, YPD slant medium and MRS slant medium, and cultivate them at 30°C-37°C for 18h-24h until obvious colonies grow on the slant. kind of activation;

[0012] (2) Pick a ring from the activated strains of Bacillus subtilis and Saccharomyces cerevisiae, inoculate them into beef extract peptone liquid medium and YPD liquid medium respectively, and culture them on a shaking table at a temperature of 30°C-37°C for 18h-48h , until the concentration of microorganisms in the culture solution reaches 106CFU / mL-108CFU / mL, the culture is stopped, and the seed solution is successfully prepared. Pick a ring from the activated strain Lactobacillus plantarum, inoculate it into MRS liquid medium, an...

Embodiment 2

[0019] The difference from Example 1 is that the aerobic fermentation is 24 hours, the sealed anaerobic fermentation is 72 hours, the inoculum amount of the Bacillus subtilis seed liquid is 5.4%, and the inoculum amount of the seed liquid of Saccharomyces cerevisiae and Lactobacillus plantarum is 1.8%. The nutritional components of soybean meal before and after fermentation were analyzed as follows:

[0020] Test result 2

[0021]

[0022] Remarks: The contents of the above substances are calculated as dry matter.

Embodiment 3

[0024] The difference from Example 2 is that the initial pH of the soybean meal is 6.5, the inoculum amount of the Bacillus subtilis seed solution is 6%, and the inoculum amount of the Saccharomyces cerevisiae and Lactobacillus plantarum seed solution is 3%. The nutritional components of soybean meal before and after fermentation were analyzed as follows:

[0025] Test result 3

[0026]

[0027]

[0028] Remarks: The contents of the above substances are calculated as dry matter.

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Abstract

The invention discloses a method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal, and relates to the technical field of farm products bioprocess. A stain of high-yield protease bacillus subtilis which is sorted from conventional Jiangxi fermented food is used for being matched with brewing yeast and lactobacillus plantarum to carry out the mixed solid fermentation on high-temperature soybean meal, slant activation and liquid enlarge cultivation are carried out, aerobic fermentation is carried out for single inoculation, anaerobic fermentation is carried out for the mixed inoculation, then the high-temperature soybean meal is dried at the temperature of 60 DEG C to obtain a fermented soybean meal feed product in which with protein nutrients are rich, antinutritional factors are passivated almost, the palatability is improved and the bioactivity is high. The method has the advantages that the constitution of the protein and the antinutritional factors of the high-temperature soybean meal fermented product is further improved, the palatability of the fermented product is improved, and the fermented product has the characteristics of rich nutrients, low resistance and palatability.

Description

technical field [0001] The invention relates to the technical field of biological processing of agricultural products, in particular to a method for preparing a peptide-rich prebiotic feed produced by fermenting high-temperature soybean meal. Background technique [0002] At present, soybean meal for feed is mainly high-temperature soybean meal. Because high-temperature soybean meal undergoes high-temperature desolvation treatment during processing, the protein in it has a large degree of denaturation and low solubility. It also contains some anti-nutritional factors, which are difficult for animals to digest. Absorption rate is low. Fermented soybean meal is a green and efficient soybean meal processing technology. In recent years, many improvements have been made to the process of fermenting soybean meal. For example, the Chinese patent application number 200810219198.7 uses three strains of Bacillus subtilis, Saccharomyces cerevisiae and lactic acid bacteria to ferment s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00
Inventor 杨瑞金李舒宇徐张贤金妙仁张燕鹏郑婷婷赵伟
Owner JIANGNAN UNIV
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