Method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal
A production method and soybean meal technology, applied in animal feed, animal feed, application and other directions, can solve the problems of low crude protein content, low palatability and high cost, and achieve the improvement of animal growth performance, improvement of nutritional characteristics, Significant economic effect
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Embodiment 1
[0011] Example 1: The following technical scheme is adopted in the example of this specific embodiment: a method for making a peptide-rich prebiotic feed produced by fermenting high-temperature soybean meal. Take one ring from Lactobacillus plantarum and put them on beef extract peptone slant medium, YPD slant medium and MRS slant medium, and cultivate them at 30°C-37°C for 18h-24h until obvious colonies grow on the slant. kind of activation;
[0012] (2) Pick a ring from the activated strains of Bacillus subtilis and Saccharomyces cerevisiae, inoculate them into beef extract peptone liquid medium and YPD liquid medium respectively, and culture them on a shaking table at a temperature of 30°C-37°C for 18h-48h , until the concentration of microorganisms in the culture solution reaches 106CFU / mL-108CFU / mL, the culture is stopped, and the seed solution is successfully prepared. Pick a ring from the activated strain Lactobacillus plantarum, inoculate it into MRS liquid medium, an...
Embodiment 2
[0019] The difference from Example 1 is that the aerobic fermentation is 24 hours, the sealed anaerobic fermentation is 72 hours, the inoculum amount of the Bacillus subtilis seed liquid is 5.4%, and the inoculum amount of the seed liquid of Saccharomyces cerevisiae and Lactobacillus plantarum is 1.8%. The nutritional components of soybean meal before and after fermentation were analyzed as follows:
[0020] Test result 2
[0021]
[0022] Remarks: The contents of the above substances are calculated as dry matter.
Embodiment 3
[0024] The difference from Example 2 is that the initial pH of the soybean meal is 6.5, the inoculum amount of the Bacillus subtilis seed solution is 6%, and the inoculum amount of the Saccharomyces cerevisiae and Lactobacillus plantarum seed solution is 3%. The nutritional components of soybean meal before and after fermentation were analyzed as follows:
[0025] Test result 3
[0026]
[0027]
[0028] Remarks: The contents of the above substances are calculated as dry matter.
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